Uppermost Layer Research Articles

SuNeRF: 3D Reconstruction of the Solar EUV Corona Using Neural Radiance Fields

To understand its evolution and the effects of its eruptive events, the Sun is permanently monitored by multiple satellite missions. The optically thin emission of the solar plasma and the limited number of viewpoints make it challenging to reconstruct the geometry and structure of the solar atmosphere; however, this information is the missing link to understand the Sun as it is: a 3D evolving star. We present a method that enables a complete 3D representation of the uppermost solar layer (corona) observed in extreme ultraviolet (EUV) light. We use a deep-learning approach for 3D scene representation that accounts for radiative transfer to map the entire solar atmosphere from three simultaneous observations. We demonstrate that our approach provides unprecedented reconstructions of the solar poles and directly enables height estimates of coronal structures, solar filaments, coronal hole profiles, and coronal mass ejections. We validate the approach using model-generated synthetic EUV images, finding that our method accurately captures the 3D geometry of the Sun even from a limited number of 32 ecliptic viewpoints (∣latitude∣ ≤ 7°). We quantify the uncertainties of our model using an ensemble approach that allows us to estimate the model performance in the absence of a ground truth. Our method enables a novel view of our closest star and is a breakthrough technology for the efficient use of multi-instrument data sets, which paves the way for future cluster missions.

Synergistic binding mechanisms of co-contaminants in soil profiles: Influence of iron-bearing minerals and microbial communities

In contaminated soil sites, the coexistence of inorganic and organic contaminants poses a significant threat to both the surrounding ecosystem and public health. However, the migration characteristics of these co-contaminants within the soil and their interactions with key components, including Fe-bearing minerals, organic matter, and microorganisms, remain unclear. This study involved the collection of a 4.3-m-depth co-contaminated soil profile to investigate the vertical distribution patterns of co-contaminants (namely, arsenic, cadmium, and polychlorinated biphenyls (PCBs)) and their binding mechanisms with environmental factors. The results indicated a notable downward accumulation of inorganic contaminants with increasing soil depth, whereas PCBs were predominantly concentrated in the uppermost layer. Chemical extraction and synchrotron radiation analysis highlighted a positive correlation between the abundance of reactive iron (FeCBD) and both co-contaminants and microbial communities in the contaminated site. Furthermore, Mantel tests and structural equation modeling (SEM) demonstrated the direct impacts of FeCBD and microbial communities on co-contaminants within the soil profile. Overall, these results provided valuable insights into the migration and transformation characteristics of co-contaminants and their binding mechanisms mediated by minerals, organic matter, and microorganisms.

Niche differentiation within bacterial key-taxa in stratified surface waters of the Southern Pacific Gyre.

One of the most hostile marine habitats on Earth is the surface of the South Pacific Gyre (SPG), characterized by high solar radiation, extreme nutrient depletion, and low productivity. During the SO-245 "UltraPac" cruise through the center of the ultra-oligotrophic SPG, the marine alphaproteobacterial group AEGEAN169 was detected by fluorescence in situ hybridization at relative abundances up to 6% of the total microbial community in the uppermost water layer, with two distinct populations (Candidatus Nemonibacter and Ca. Indicimonas). The high frequency of dividing cells combined with high transcript levels suggests that both clades may be highly metabolically active. Comparative metagenomic and metatranscriptomic analyses of AEGEAN169 revealed that they encoded subtle but distinct metabolic adaptions to this extreme environment in comparison to their competitors SAR11, SAR86, SAR116, and Prochlorococcus. Both AEGEAN169 clades had the highest percentage of transporters per predicted proteins (9.5% and 10.6%, respectively). In particular, the high expression of ABC transporters in combination with proteorhodopsins and the catabolic pathways detected suggest a potential scavenging lifestyle for both AEGEAN169 clades. Although both AEGEAN169 clades may share the genomic potential to utilize phosphonates as a phosphorus source, they differ in their metabolic pathways for carbon and nitrogen. Ca. Nemonibacter potentially use glycine-betaine, whereas Ca. Indicimonas may catabolize urea, creatine, and fucose. In conclusion, the different potential metabolic strategies of both clades suggest that both are well adapted to thrive resource-limited conditions and compete well with other dominant microbial clades in the uppermost layers of SPG surface waters.

Variations in soil physico-chemical properties, soil stocks, and soil stoichiometry under different soil layers, the major forest region Liupan Mountains of Northwest China.

Liupan Mountains are an important region in China in the context of forest cover and vegetation due to huge afforestation and plantation practices, which brought changes in soil physio-chemical properties, soil stocks, and soil stoichiometries are rarely been understood. The study aims to explore the distribution of soil nutrients at 1-m soil depth in the plantation forest region. The soil samples at five depth increments (0-20, 20-40, 40-60, 60-80, and 80-100 cm) were collected and analyzed for different soil physio-chemical characteristics. The results showed a significant variation in soil bulk density (BD), soil porosity, pH, cation exchange capacity (CEC), and electric conductivity (EC) values. More soil BD (1.41 g cm-3) and pH (6.97) were noticed in the deep soil layer (80-100 cm), while the highest values of porosity (60.6%), EC (0.09 mS cm-1), and CEC (32.9 c mol kg-1) were reflected in the uppermost soil layer (0-20 cm). Similarly, the highest contents of soil organic carbon (SOC), total phosphorus (TP), available phosphorus (AP), total nitrogen (TN), and available potassium (AK) were calculated in the surface soil layer (0-20 cm). With increasing soil depth increment a decreasing trend in the SOC and other nutrient concentration were found, whereas the soil total potassium (TK) produced a negative correlation with soil layer depth. The entire results produced the distribution of SOCs and TNs (stocks) at various soil depths in forestland patterns were 0→20cm > 20→40cm > 40→60cm ≥ 60→80cm ≥ 80→100 cm. Furthermore, the stoichiometric ratios of C, N, and P, the C/P, and N/P ratios showed maximum values (66.49 and 5.46) in 0-20 cm and lowest values (23.78 and 1.91) in 80-100 cm soil layer depth. Though the C/N ratio was statistically similar across the whole soil profile (0-100 cm). These results highlighted that the soil depth increments might largely be attributed to fluctuations in soil physio-chemical properties, soil stocks, and soil stoichiometries. Further study is needed to draw more conclusions on nutrient dynamics, soil stocks, and soil stoichiometry in these forests.

Nano-protrusions in intercalated graphite: understanding the structural and electronic effects through DFT.

Complex phenomena characterize the intercalation of ions inside stratified crystals. Their comprehension is crucial in view of exploiting the intercalation mechanism to change the transport properties of the crystal or obtaining a fine control of crystal delamination. In particular, the relationship between the concentration and nature of intercalated ions and surface structural modifications of the host stratified crystal is still under debate. Here, we discuss a theoretical effort to provide a rationale for some structural changes observed on the highly oriented pyrolytic graphite (HOPG) surface after electrochemical treatment in perchloric and sulphuric acid solutions. The formation of the so-called nano-protrusions on the basal plane of intercalated graphite was previously observed with scanning tunneling microscopy (STM). In this work, we employed both STM and density functional theory (DFT) simulations to elucidate the physical and chemical mechanisms driving the emergence of these nano-protrusions. The DFT results show that, in a bilayer graphene system, the presence of a single ion can generate a nano-protrusion with 2.49 Å height and 21.27 Å width. In the deformed area, the C-C bond length is stretched by about 2.5% more than the normal graphene bond. These values are of the same dimensional scale as those reported in previous STM experimental results.25 However, the simulated STM images obtained by increasing the amount of intercalated ions per area suggest that the presence of more than one ion is needed for the deformation of the uppermost graphite layer during the early stages of intercalation. In contrast, in a multilayer graphene system, no significant surface deformation is detected when ions are intercalated between the third and fourth layers. Charge analysis indicates an altered distribution of the charges as a consequence of the intercalation. The charge transfer from graphene layers to the intercalated ions results in a surface layer more prone to oxidation.

Dune Blowouts as Microbial Hotspots and the Changes of Overall Microbial Activity and Photosynthetic Biomass Along with Succession of Biological Soil Crusts

Biological soil crust (BSC) constitutes a consortium of cyanobacteria, algae, lichen, mosses, and heterotrophic microorganisms, forming a miniature ecosystem within the uppermost soil layer. The biomass of different organisms forming BSC and their activity changes along with succession. Previous studies focused primarily on BSC in hyper-arid/arid regions, whereas the ecophysiology of BSC in temperate climates is still not well recognized. In order to determine changes in overall microbial activity and photosynthetic biomass in BSC at different stages of the succession of inland sandy grasslands, we analyzed dehydrogenase activity and determined the content of photosynthetic pigments. We also compared these parameters between BSC developed on the dune ridges and aeolian blowouts in the initial stage of succession. Our study revealed a significant increase in both photosynthetic biomass and overall microbial activity in BSC as the succession of inland shifting sands progresses. We found that chl a concentration in BSC could be considered a useful quantitative indicator of both the presence of photoautotrophs and the degree of soil crust development in warm-summer humid continental climates. The photosynthetic biomass was closely related to increased microbial activity in BSC, which suggests that photoautotrophs constitute a major BSC component. Dune blowouts constitute environmental niches facilitating the development of BSC, compared to dune ridges. High biomass of microorganisms in the dune blowouts may be associated with a high amount of organic material and more favorable moisture conditions. We conclude that deflation fields are key places for keeping a mosaic of habitats in the area of shifting sands and can be a reservoir of microorganisms supporting further settlement of dune slopes by BSC.

Variability of rare earth and heavy metal elements in a representative alluvial depositional system.

Heavy metals (Ni, Cr, W, Cd, and Pb) and rare earth elements (REE) were investigated in the flood plain sediments of an island of the lower Yangtze River near Nanjing to determine how the vertical distribution of heavy metals could be affected by natural sedimentation processes and anthropogenic contamination. Stratigraphic analyses of magnetic susceptibility and the mean grain size distribution of the deposits enabled us to identify layers associated with a relatively high influx of suspended sediments that resulted in sudden changes in the concentrations of heavy metals. The results show that layers associated with high sediment influx (0.8m depth) displayed low concentrations of Cr, Ni, W, and Cd that were mainly lithogenic in origin. The Post Archean Australian Shale (PAAS) normalized REE patterns in the flood plain cores were enrichment in Ce and Eu relative to PAAS, indicating that the sediments were most likely derived from a mixture of sediments and not from an anthropogenic source. Sharp increases in Y/Ho ratios, as well as heavy metal (Cd, Cr, Ni, and W) and Y concentrations were observed in the uppermost layer that could have been deposited from the rapid transport of sediment-laden, contaminated waters. The temporal (vertical) trends in Pb concentrations may be strongly influenced by coal burning. Elevated Pb concentrations (350ppm and 1000ppm) correlate with high magnetic susceptibility (> 200 m3 × kg-1) and the history of thermal power plant (1910-2002) activity. The anthropogenic inputs of Pb were, however, not diluted by high suspended sediment loads, which supports the argument that Pb was derived from fly ash.

The hypothesis of the shape of the permafrost in Hornsund, Spitsbergen and the potential impact of its degradation on the Arctic

The areas covered by permafrost in the polar regions are vulnerable to rapid changes in the current climate. The well-studied near-surface active layer and permafrost zone are in contrast to the unknown exact shape of the bottom permafrost boundary. Therefore, the entire shape of permafrost between the upper and lower boundaries is not identified with sufficient accuracy. Since most of the factors affecting deep cryotic structures are subsurface in nature, their evolution in deeper layers is also relatively unclear. Here, we propose a hypothesis based on the results of geophysical studies regarding the shape of the permafrost in the coastal area of Svalbard, Southern Spitsbergen. In the article, we emphasize the importance of recognizing not only the uppermost active layer but also the bottom boundary of permafrost along with its transition zone, due to the underestimated potential role of its continuity in observing climate change. The lower permafrost boundary is estimated to range from 70 m below the surface in areas close to the shore to 180 m inland, while a continuous layer of an entirely frozen matrix can be identified with a thickness between 40 m and 100 m. We also hypothesized the presence of the possible subsea permafrost in the Hornsund. The influence of seawater intrusions, isostatic uplift of deglaciated areas, and surface-related processes that affect permafrost evolution may lead to extensive changes in the hydrology and geology of the polar regions in the future. For all these reasons, monitoring, geophysical imaging and understanding the characteristics and evolution of deep permafrost structures requires global attention and scientific efforts.

Integration of GIS with the Generalized Reciprocal Method (GRM) for Determining Foundation Bearing Capacity: A Case Study in Opolo, Yenagoa Bayelsa State, Nigeria

This study addresses the pressing need to assess foundation bearing capacity in Opolo, Yenagoa, Bayelsa State, Nigeria. The significance lies in the dearth of comprehensive geotechnical data for construction planning in the region. Past research is limited and this study contributes valuable insights by integrating Geographic Information System (GIS) with the Generalized Reciprocal Method (GRM). To collect data, near-surface seismic refraction surveys were conducted along three designated lines, utilizing ABEM Terraloc Mark 6 equipment, Easy Refract, and ArcGIS 10.4.1 software. This methodology allowed for the determination of key geotechnical parameters essential for soil characterization at potential foundation sites. The results revealed three distinct geoseismic layers. The uppermost layer, within a depth of 0.89 to 1.50 meters, exhibited inadequate compressional and shear wave velocities and low values for oedometric modulus, shear modulus, N-value, ultimate bearing capacity, and allowable bearing capacity. This indicates the presence of unsuitable, soft, and weak alluvial deposits for substantial structural loads. In contrast, the second layer (1.52 to 3.84 m depth) displayed favorable geotechnical parameters, making it suitable for various construction loads. The third layer (15.00 to 26.05 m depth) exhibited varying characteristics. The GIS analysis highlighted the unsuitability of the uppermost layer for construction, while the second and third layers were found to be fairly competent and suitable for shallow footing and foundation design. In summary, this study highlights the importance of geotechnical surveys in Opolo’s construction planning. It offers vital information for informed choices, addresses issues in the initial layer, and suggests secure, sustainable construction options.

Similarity preserving hashing for appliance identification based on V-I trajectory

Non-intrusive load monitoring (NILM) is a technique used to monitor energy consumption in buildings without requiring hardware installation on individual appliances. This approach offers a cost-effective and scalable solution to enhance energy efficiency and reduce energy usage. Recent advancements in NILM primarily employ deep-learning algorithms for appliance identification. However, the substantial number of parameters in deep learning models presents challenges in quickly and effectively identifying appliances. An effective technique for appliance identification is analyzing the appliances’ voltage-current (V-I) trajectory signature. This research introduces a novel hashing method that learns compact binary codes to achieve highly efficient appliance V-I trajectory identification. Specifically, this paper uses a profound structure to acquire V-I trajectory image features by acquiring multi-level non-linear transformations. Subsequently, we merge these intermediary traits with high-level visual data from the uppermost layer to carry out the V-I trajectory image retrieval process. These condensed codes are subjected to three distinct standards: minimal loss in quantization, uniformly distributed binary components, and autonomous bits that are not interdependent. As a result, the network easily encodes newly acquired query V-I images for appliance identification by propagating them through the network and quantizing the network’s outputs into binary code representations. Through extensive experiments conducted on the PLAID dataset, we demonstrate the promising performance of our approach compared to state-of-the-art methods.

Mapping Leachate Pathways in Aging Mining Tailings Pond Using Electrical Resistivity Tomography

Mining activities often leave behind a legacy of environmental challenges, with aging tailings ponds representing a significant concern due to their potential for leachate formation and subsequent contaminant release. Thus, this study employs Electrical Resistivity Tomography (ERT) to investigate the intricate pathways of leachate within an aging mining tailings pond, addressing the pressing environmental and human health concerns associated with potential contaminant release. Ten 2D ERT profiles were acquired at the El Mochito mine waste site, covering an area of approximately half a square kilometer. These profiles, ranging in length from 104 to 363 m, provided insights into subsurface conditions down to a maximum depth of 60 m. The subsurface mapping of the ERT data showed three different geoelectric layers. The uppermost layer, with a thickness of approximately 2.5 m and resistivity values ranging from 60 to 100 Ohm.m, was identified as a dry tailing/soil zone. Beneath it, the second layer exhibited moderately resistive values (30–60 Ohm.m) with varying thicknesses of 10–20 m, signifying a percolation/leaching zone (semi-saturated zone). The third layer, characterized by substantially low resistivity (1–30 Ohm.m), indicated saturation and the presence of conductive materials, strongly suggesting active leaching. Based on these findings, this study recommends further investigation through geochemical analysis of subsurface samples and more advanced geophysical imaging techniques to validate the distribution of anomalous zones and delineate remediation pathways. This study lays the foundation for future comprehensive research that will integrate geophysical surveys with geochemical analysis and establish 4D modeling techniques to monitor pollutant penetration over time, with a particular focus on mine waste tailings mapping. Plus, this study contributes valuable insights into the characterization of leachate pathways within mining tailings ponds, offering a foundation for informed environmental management and remediation strategies.

Side-effects of laser weeding: quantifying off-target risks to earthworms (Enchytraeids) and insects (Tenebrio molitor and Adalia bipunctata)

With challenges posed by chemical and mechanical weed control, there are now several research and commercial projects underway to develop autonomous vehicles equipped with lasers to control weeds in field crops. Recognition systems based on artificial intelligence have been developed to locate and identify small weed seedlings, and mirrors can direct a laser beam towards the target to kill the weed with heat. Unlike chemical and mechanical weed control, laser weeding only exposes a small area of the field for the treatment. Laser weeding leaves no chemicals in the field after the treatment or does not move the soil which may harm crop roots and non-target organisms. Yet, it is well-known that laser beams can harm living organisms; the effect on the environment and fauna should be studied before laser weeding becomes a common practice. This project aimed to study the effect of laser on some living non-target organisms. We investigated the effect of laser treatment on the mortality of two species of earthworms (Enchytraeus albidus and Enchytraeus crypticus), larvae, pupas, and beetles of yellow mealworm beetles (Tenebrio molitor) and the two-spotted lady beetle (Adalia bipunctata) for increasing dosages of laser energy. In all earthworms experiments except one, the mortality rates of the worms living in the uppermost soil layer of clay, sandy, and organic soil exposed to laser heating were not significantly different from the controls even with laser dosages up to 23.8 J mm-2. Laser doses sufficient to kill plants were lethal to the insects, and lower doses that did not kill plants, killed or harmed the insects across all life stages tested. The larger beetles survived higher doses than smaller. Laser weeding is a relatively new technology and not yet widely practiced or commercialized. Therefore, we do not discuss and compare the costs of the different weeding methods at this early stage of the development of the technology.

Development of a novel in vitro strategy to understand the impact of shaving on skin health: combining tape strip exfoliation and human skin equivalent technology.

The removal of unwanted hair is a widespread grooming practice adopted by both males and females. Although many depilatory techniques are now available, shaving remains the most common, despite its propensity to irritate skin. Current techniques to investigate the impact of shaving regimes on skin health rely on costly and lengthy clinical trials, which hinge on recruitment of human volunteers and can require invasive biopsies to elucidate cellular and molecular-level changes. Well-characterised human skin equivalent technology was combined with a commonplace dermatological technique of tape stripping, to remove cellular material from the uppermost layer of the skin (stratum corneum). This method of exfoliation recapitulated aspects of razor-based shaving in vitro, offering a robust and standardised in vitro method to study inflammatory processes such as those invoked by grooming practices. Tape strip insult induced inflammatory changes in the skin equivalent such as: increased epidermal proliferation, epidermal thickening, increased cytokine production and impaired barrier function. These changes paralleled effects seen with a single dry razor pass, correlated with the number of tape strips removed, and were attenuated by pre-application of shaving foam, or post-application of moisturisation. Tape strip removal is a common dermatological technique, in this study we demonstrate a novel application of tape stripping, to mimic barrier damage and inflammation associated with a dry shave. We validate this method, comparing it to razor-based shaving in vitro and demonstrate the propensity of suitable shave- and skin-care formulations to mitigate damage. This provides a novel methodology to examine grooming associated damage and a platform for screening potential skin care formulations.

Germination improvement of fenugreek seeds with cold plasma: Exploring long-lasting effects of surface modification

Pre-sowing cold plasma (CP) treatment of seeds is a proven method for enhancing seed germination and plant growth. This paper presents a study on the long-lasting effects of CP treatment on fenugreek (Trigonella foenum-graecum) seeds to elucidate the basic mechanism of plasma action and pave the way for future field experiments. The seeds were subjected to short-duration treatments (10 and 20 s) using the kINPen 11 plasma jet and then stored for 3 months before sowing. After this period, the treated seeds exhibited higher germination efficiency, and the resulting seedlings were longer and more vigorous compared to normal seedlings. The improvement in germination was particularly noticeable for a treatment time of 20 s. Although we observed the age-related hydrophobic recovery, the plasma-treated seeds consistently showed better wettability than the control group. However, no direct correlation was found between wettability and germination improvement. Scanning electron microscopy (SEM) analysis revealed increased surface roughness and microstructuring of the outer layers of the seed coat after plasma treatment, while X-ray photoelectron spectroscopy (XPS) indicated an increase in the O/C ratio, as well as the presence of calcium and potassium. These findings suggest that the argon plasma etched the uppermost layer of the seed coat, leading to structural changes. It is suspected that the observed faster germination and growth of plasma-treated seeds is seeds due to these long-lasting changes in the seed coat structure, which may disrupt its tissues and open access to the interior of the seed. Under appropriate conditions, this facilitates an increase in the metabolic activity of the embryo. Such a mechanism can be considered as plasma scarification of the seed. This study highlights the potential of cold plasma technology as a viable and simple strategy for improving seed germination and plant growth in the long term, with important implications for the agricultural sector.

Soil Fertility and Phosphorus Leaching in Irrigated Calcareous Soils of the Mediterranean Region

To ensure soil quality and soil health, it is necessary to improve fertilization practices while minimizing environmental impacts. The aim of this study was to record the state of the art on soil fertility related to fertilization management (organic and/or mineral) and to detect environmental challenges in highly productive fields. A soil survey was set up in a new irrigated area (c. 20 years old), in the north-eastern part of Spain, which is mainly devoted to double annual crop rotations of cereals and maize. The area also supports an important animal rearing activity. The survey covered 733 ha of calcareous soils, owned by 35 farmers. At each farm, fertilization management was recorded, and soil was analyzed for nutrients and heavy metals. Multivariate analyses were performed. Total N, P, Cu and Zn, and available P, Cu, Zn and Mn soil concentrations were associated to the use of organic amendments. Heavy metals concentrations were below established thresholds. Available P (Olsen-P) was identified as an indicator of the previously adopted fertilization management and of the potential of P leaching towards deeper soil layers. Regression analyses were performed. A displacement of available P from the uppermost layer (0–0.3 m) occurs in the breakpoint of 86 mg P kg−1 soil. Preventative actions might be established from 53 mg P kg−1 soil due to the slowdown in P immobilization. Our results reinforce the importance of setting up P threshold soil levels for best practices of fertilization, as a basis for sustainable agriculture intensification.

Pedogenic controls of soil organic carbon stocks and stability beneath montane Norway spruce forests along a precipitation gradient

Reliable data on SOC stocks in forest soils is required in the context of climate change and soil health assessments but still limited by input data availability (e.g., bulk density) and methods used for stock calculation. Relatively few studies have investigated the stability of SOC in forest soils. We investigated SOC stocks and fractionation in soils beneath Norway spruce forests and grasslands in the montane zone along a gradient of mean annual precipitation (MAP). We sampled soil cores volumetrically to 40 cm depth and measured SOC in the fractions <2 mm (fine earth), >200 μm and 200–20 μm (coarse and fine POM), and <20 μm (MAOM) along with potential pedogenic controls. Total SOC stocks beneath forests in the study region, calculated by the equivalent soil mass (ESM) approach to 40 cm depth, amount to 79.0 ± 29.9 (mean ± standard deviation) Mg ha−1 (n = 20) in the mineral soil, and to 92.9 ± 30.6 Mg ha−1 including the litter layer, with a share of 55 % associated with POM. MAOM makes up ∼41 % of SOC in the uppermost mineral layer (0–5 cm) and increases to 71 % in the subsoil (20–40 cm). Multiple regression models show that MAOM is largely controlled by ammonium oxalate extractable Al (Alo) in the forest subsoils (20–40 cm), and increases with MAP in the topsoil layers (0–20 cm). Soils on carbonate rock stand out with ∼80–100 % larger shares of MAOM in the uppermost soil layers (0–10 cm) which is likely connected to higher soil pH and MAP, supporting microbial transformation and subsequent stabilisation of organic matter, which is reflected in narrower C:N ratios in MAOM and SOC.Including the litter layers, ESM-based total SOC stocks in forest soils tend to exceed those beneath grassland (80.2 ± 21.9 Mg ha−1; n = 31) by 16 %, but only by 6.4 % if calculated by the conventional fixed-depth (FD) approach. In contrast to the forest soils, SOC stocks beneath grasslands are dominated by MAOM (75.6 %).We conclude that (coniferous) forest soils are a poor reference for establishing sequestration potentials for stable SOC. The observed large proportion of POM in forest topsoils and its increase with declining MAP (indicating water availability) suggests a risk of SOC losses in response to increasing droughts due to climate change.

Soil Abandonment as a Trigger for Changes in Zn Fractionation in Afforested Former Vineyard Acidic Soils

Zinc is an essential element for plant nutrition, but it may cause toxicity depending on its bioavailability and potential transformation in soil. In vineyard soils, high concentrations of Zn are usually found, mainly due to agricultural practices. However, a great abandonment of vineyards has recently occurred, leading to changes in the total and bioavailable Zn concentrations, as well as Zn fractionation. We analyzed Zn concentrations (total, ZnT, and bioavailable, ZnED) and fractionation in the soil of three paired sites (PM, PT, and AR) up to depths of 50 cm in active and adjacent abandoned vineyards that were already transformed into forests. The ZnT averaged at 210 mg kg−1 among all studied vineyards. The results showed changes in the vertical pattern ZnT concentrations after vineyard abandonment at the PM and PT sites, while at the AR site, no great variation occurred. The ZnED (mean values = 7 mg kg−1) decreased after abandonment at PM and AR in the uppermost surface layers, while it increased in the top 10 cm at the PT site, reaching up to 60 mg kg−1. Regarding Zn fractionation in active vineyards, the residual fraction (ZnR) was the most abundant, followed by Zn bound to crystalline Fe and Al oxy-hydroxides (ZnC) and Zn bound to soil organic matter (ZnOM). After abandonment, the ZnR slightly increased and the ZnC slightly decreased at the PM and AR sites at all depths, while the ZnOM showed a noticeable variation in the uppermost 10 cm of the PT site. These results suggest that the soil organic matter that is provided during afforestation may play an important role in Zn fractionation and mobilization, depending on its humification degree and chemical stability. Zn mobilization could result in a positive nutrient supply for plants, but caution must be taken, since an excess of Zn could cause toxicity in long-term abandoned vineyards.

Mercury mobilization in shrubland after a prescribed fire in NE Portugal: Insight on soil organic matter composition and different aggregate size

Soils constitute the major reservoir of mercury (Hg) in terrestrial ecosystems, whose stability may be threatened by wildfires. This research attempts to look at the effect of prescribed fire on the presence of Hg in a shrubland ecosystem from NE Portugal, delving into its relationship with soil aggregate size and the molecular composition of soil organic matter (SOM). During the prescribed fire, on average 347 mg Hg ha−1 were lost from the burnt aboveground biomass of shrubs and 263 mg Hg ha−1 from the combustion of the soil organic horizon. Overall, Hg concentration and pools in the mineral soil did not show significant changes due to burning, which highlights their role as long-term Hg reservoirs. The higher Hg concentrations found in smaller aggregates (<0.2 mm) compared to coarser ones (0.5–2 mm) are favored by the higher degree of organic matter decomposition (low C/N ratio), rather than by greater total organic C contents. The Hg-enriched finest fraction of soil (<0.2 mm) could be more prone to be mobilized by erosion, whose potential arrival to water bodies increases the environmental concern for the Hg present in fire-affected soils. The SOM quality (molecular composition) and the main organic families, analyzed by Fourier-transform infrared spectroscopy in combination with multivariate statistical analysis, significantly conditioned the retention/emission of Hg in the uppermost soil layers. Thus, before the fire, Hg was strongly linked to lipid and protein fractions, while Hg appeared to be linked to aromatic-like compounds in fire-affected SOM.

Stacking Faults and Topological Properties in MnBi2Te4: Reconciling Gapped and Gapless States.

Despite theoretical predictions of a gapped surface state for the magnetic topological insulator MnBi2Te4 (MBT), there has been a series of experimental evidence pointing toward gapless states. Here, we theoretically explore how stacking faults could influence the topological characteristics of MBT. We envisage a scenario that a stacking fault exists at the surface of MBT, causing the uppermost layer to deviate from the ground state and its interlayer separation to be expanded. This stacking fault with modulated interlayer couplings hosts a nearly gapless state within the topmost layer due to charge redistribution as the outermost layer recedes. Furthermore, we find evidence of spin-momentum locking and preservation of weak band inversion in the gapless surface state, suggesting the nontrivial topological surface states in the presence of the stacking fault. Our findings provide a plausible elucidation to the long-standing conundrum of reconciling the observation of gapped and gapless states on MBT surfaces.

Overcoming the barrier of skin to drug permeation for localized dermatological therapies

Background. The skin’s uppermost layer, the stratum corneum is a very effective barrier against the penetration of compounds including pharmaceuticals and cosmetic actives. To deliver higher amounts of drugs into the skin layers or to deliver drugs deeper into the skin (e.g., into the dermis), several enhancement techniques have been established. These techniques include chemical penetration enhancers as well as physical techniques such as iontophoresis and microneedles. In addition, one of the newer approaches includes the use of nano-based carriers such as metallic nanoparticles and polymeric self-assembling nanospheres. Methods. This mini-review explores this new approach of using nano-based drug carriers for skin penetration enhancement. In particular we will explore the use of gold nanoparticles as well as biocompatible tyrosine-derived polymeric nanoparticles known as Tyrospheres. Results. The most investigated carriers in the class of metallic carriers are gold nanoparticles that can be used for both medical as well as diagnostic uses. Many investigators have reported that gold nanoparticles are able to enhance the skin transport and delivery of macromolecular and hydrophilic drugs. Meanwhile, for challenging highly lipophilic and/or unstable compounds such as adapalene and Vitamin D3 packaging them into polymeric nanocarriers such as Tyrospheres enables drug delivery to hair follicles, significantly increased aqueous solubility and resulted in elevated amounts of drug in targeted skin layers. Conclusions. The relatively new approach of using nanotechnological approaches as a way of enhancement of drug delivery to skin shows significant promise over some other established techniques such as the addition of chemical penetration enhancers to formulations used for topical/transdermal uses.