Unwanted Activity Research Articles

THE ETIOLOGY OF PERPETRATOR OF CHILDREN SEXUAL VIOLENCE: A CASE STUDY IN THE PPA UNIT IN THE POLICE STATION OF SUMBAWA REGENCY

Sexual violence is any verbal and nonverbal action to manipulate another person to make the person engage in unwanted sexual activity. Recently, sexual violence against children in Sumbawa Besar District raised the concerns of various groups. This is due to the increasing number of cases of sexual violence against children. The purpose of this research is to find out causes (the etiology) of the perpetrators in comitting sexual violence. The method used in this research is qualitative research with the case study approach. The data were collected using interview and observation methods. The subjects of this study were perpetrators of sexual violence in Sumbawa district. The data credibility was examined with triangulation of source, technique, and theory. The four stages of data analysis were data collection, data sortation, data conclusions, and data verification. The results showed that there were various reasons of the perpetrators to commit sexual violence towards children. Some of the factors are problems in their families and the need and opportunity to fulfill their sexual desires.

Protective effect of Phytochemicals against Cisplatin induced Nephrotoxicity

Cisplatin is an inorganic platinum-based oncologic medication and has a broad spectrum of uses in the therapeutic management of number of solid malignant tumour. FDA approved the clinical use of cisplatin in the year 1978. Since then, it has been used alone or in combination with other drugs in chemotherapy. Though, it has highly cured rate for the treatment of cancer, the use of cisplatin is limited due to its major dose limiting side effects such as nephrotoxicity and ototoxicity. The development of cisplatin nephrotoxicity is complex and a number of interrelated factors such as transporter mediated cisplatin accumulation, conversion into nephrotoxins, formation of DNA adducts, mitochondrial dysfunction, nitrosative and oxidative stress, inflammation, signal transducers and apoptotic pathway activation are involved. A number of synthetic drugs are available for the management of cisplatin toxicity but associated with a number of serious side effects such as hypotension, ototoxicity, nausea, vomiting and decreased calcium levels. In addition, various reports show that most of these compounds show unwanted tumour protective activity. Literature review suggested that phytochemicals are reported to have preventive activity in CIRT and it is evident that these compounds showed a pronounced renoprotective activity against CIRT. Therefore, in this review, we highlight the role of the phytochemicals, which are shown to be efficacious in clinically

Albemarle catalysts reports 1Q 2021 sales growth

The speciation of Ni in spent Fluid Catalytic Cracking catalysts is studied. Carefully performed XRD analyses with NiO/SiO2 samples used as calibration standards, demonstrate that the presence of NiO as a separate phase is reliably detected also for NiO concentration close to 0.1 wt% (1000 ppmw). Actually, both on real spent FCC catalysts (ECAT's) and on artificially contaminated industrial FCC catalysts, NiO is not detectable as a separate phase, even after contamination of the order of 15,000 ppmw of Ni. SEM–EDS experiments provide evidence of a largely preferential location of Ni on alumina particles present in the FCC catalyst mixture, thus acting as “nickel traps”. XRD, UV–vis and TPR data confirm that Ni deposits on alumina particles forming a hardly reducible surface and subsurface layer, giving rise to a solid whose composition is NixAl2O3+x with x << 0.25. As a result of this, the formation of extended Ni metal particles in the raiser reactor is avoided, being their unwanted dehydrogenation activity essentially eliminated. The absence of bulk NiO in the spent FCC catalysts (at least when Ni content is in the range 2000–10,000 ppmw) allows the classification of this material as a non-hazardous waste.

Microbial stabilisation of white wine by filtration through silica microparticles functionalised with natural antimicrobials

During wine production and storage, undesired effects can appear mainly due to the microbiological activity of the present microorganisms leading to economic losses. Wine stabilisation is needed for controlling the unwanted microbiological activity to improve wine safety and final quality. Nowadays, a plethora of viticultural and technological solutions is available but, in some cases, the stabilisation process changes the wine sensory profile. The aim of this study was the development of filter aids functionalised with phenolic compounds (PHE) with enhanced antimicrobial properties. The filters’ removal capability was evaluated using white wine inoculated with Acetobacter aceti, Lactobacillus plantarum, Dekkera bruxellensis, Zygosaccharomyces bailii and Saccharomyces cerevisiae. Wine was filtered through PHE-coated filters and the microbial load was determined by plate count. The influence of filtration on the physicochemical wine parameters was also assessed. The removal capacity results showed the PHE-functionalised filters were capable of reducing 3 logarithmic values, being eugenol the most effective compound. The results evidenced different influence of filtration on wine properties according to the immobilised PHE showing that eugenol-functionalised supports had a very low impact on physicochemical parameters. Thus our results support the relevance of using antimicrobial-coated filters at different stages of the winemaking process for wine microbiological stabilisation.

HUBUNGAN FAKTOR INTERNAL PACAR DENGAN KEKERASAN DALAM PACARAN

Many women are victims of violence by their boyfriends. The act of violence can be in the form of being overly coercive and controlling their partner. This is due to the influence of consuming alcohol. The age of Boyfriend is also associated with premature sexual violence and unwanted sexual activity in adolescents. The purpose of this study was to determine the relationship between factors internal of boyfriends and violence in dating. The research method was observational analytic with cross sectional approach.&#x0D; The sampling technique was used purposive, with a sample of 99 respondents. The result of the study showed that include :. There is no relationship between internal factors of boyfriends when viewed from age with a value of p = 0.145 &lt;0.05.There is a very significant positive relationship with factor internal of boyfriend when viewed from the use of alcohol with a value of p = 0.00 and a moderate correlation value. Recommendations for future research can be examined on a larger population and sample to obtain the accuracy of data, and can examine other factors such as the length of dating relationships with the incidence of dating violence.

Persons with Intellectual Disability: Sexual Behaviour, Knowledge and Assertiveness.

BackgroundPersons with ID most often have incomplete, contradictory and imprecise knowledge of sexuality and sexual intercourse itself. They are not provided with sufficient information on their own body and sexuality, and are often discouraged from and sanctioned for trying to sexually express themselves. Sexual abuse due to low sexual assertiveness is also common.AimThe principal aim of this study was to establish the presence or absence of sexual activity in adults with ID residing in institutional housing, as well as the level and structure of their knowledge of sexuality, their sexual assertiveness and preparedness to react in a sexually dangerous situation.MethodsThe sample consisted of 100 participants with ID residing in institutional housing. The instruments used included the General Sexual Knowledge Questionnaire, What-if test and Hulbert index of sexual assertiveness. Comparative statistics included coefficient of linear correlation and multiple regression analysis.ResultsThe results showed that 82% of the participants are sexually active. Most participants admitted to sometimes having sexual intercourse against their wishes as well as to having difficulty asserting themselves. Their knowledge of pregnancy, contraception and sexually transmitted diseases was very low. Female participants and those that reported having sexual intercourse had more sexual knowledge and were also more sexually assertive.ConclusionKnowledge of sexuality and sexual assertiveness of persons with ID residing in institutional housing is very low. Additional information on sexuality is necessary, as well as support in learning to express their own desires and to deal with unwanted sexual activity.

#MeToo for Whom? Sexual Assault Disclosures Before and After #MeToo

In October 2017, #MeToo became a global hashtag for victims of sexual assault and harassment. In this study, we examine the extent of unwanted sexual experiences and disclosure, as well as perceptions of #MeToo, to assess differences among students who were represented and underrepresented in #MeToo coverage. Using a stratified random sample of students at a private university in the mid-Atlantic region of the U.S., we analyzed data from cross-sectional surveys conducted in March 2017 (n = 1722) and March 2019 (n = 1503). Results suggest that, relative to White students, in 2019 compared to 2017, there was an increase in disclosures among Black students and a decrease among Multiracial students. There was also an increase in undergraduate students who indicated ever experiencing unwanted sexual activity in 2019. Students who believed #MeToo affected how they think about past experiences were more likely to have indicated past experiences with unwanted sexual activity. Results also suggest that cisgender women, LGBQ, white, and multiracial students are at increased risk of unwanted sexual activity. However, Asian students, cisgender men, and non-LGBQ students may be less likely to disclose unwanted sexual activity. Implications for evaluating long-term impacts of #MeToo are discussed, along with suggestions for future research.

Three-Tier Control of Energy Costs in Transport Corporation with Self-Learning

According to the concept of Industry 4.0, the control of energy costs in a large transport corporation should be based on the use of artificial intelligence, the main focus of which is machine learning. It is also necessary to take into account the human factor - the unwanted activity of corporation employees who use information available only to them to achieve their own goals. The article examines the problem of reducing energy costs in a 3-tier transport corporation, which has regional offices, which are subordinate to local enterprises. The plant management knows own abilities of energy savings better than the regional management. Respectively, regional management knows these abilities better than the corporate governance. Therefore, both regional and plant management can manipulate energy expenditures to get more stimuli from the corporate governance. Such unwanted activity can result in excess energy consumption by the corporation. The goal is to minimize the energy costs of the corporation. To achieve this goal, a mechanism for organizational control of energy costs in a 3-tier transport corporation is proposed. This mechanism includes multilevel machine learning and stimulating procedures. Sufficient conditions are found for the synthesis of such a mechanism in which the existing possibilities of reducing energy costs in a transport corporation are used. The advantage of this mechanism over existing methods of learning classification in organizational systems (such as artificial neural networks or self-organizing maps) is to stimulate energy savings. The use of such a mechanism is illustrated by the example of 3-tier control system for energy consumption of JSC Russian Railways.

Machine learning techniques based on security management in smart cities using robots.

Nowadays, the growth of smart cities is enhanced gradually, which collects a lot of information and communication technologies that are used to maximize the quality of services. Even though the intelligent city concept provides a lot of valuable services, security management is still one of the major issues due to shared threats and activities. For overcoming the above problems, smart cities' security factors should be analyzed continuously to eliminate the unwanted activities that used to enhance the quality of the services. To address the discussed problem, active machine learning techniques are used to predict the quality of services in the smart city manages security-related issues. In this work, a deep reinforcement learning concept is used to learn the features of smart cities; the learning concept understands the entire activities of the smart city. During this energetic city, information is gathered with the help of security robots called cobalt robots. The smart cities related to new incoming features are examined through the use of a modular neural network. The system successfully predicts the unwanted activity in intelligent cities by dividing the collected data into a smaller subset, which reduces the complexity and improves the overall security management process. The efficiency of the system is evaluated using experimental analysis. This exploratory study is conducted on the 200 obstacles are placed in the smart city, and the introduced DRL with MDNN approach attains maximum results on security maintains.

Incremental Value of Post Diuretic 68Ga-PSMA-11 PET-CT in Characterization of Indeterminate lLesions in Prostate Cancer.

Objective:The aim of current study is to evaluate the role of diuretic assisted 68Ga-PSMA PET-CT, on image quality and clinical interpretation of indeterminate/equivocal lesions in pre-Lasix imaging of Prostate cancer. Materials and Methods:Forty-five patients underwent baseline 68Ga-PSMA-11 scan 45-60 minutes post tracer injection followed by post Lasix study after ±15 minutes. The contrast to noise ratios (CNR), noise and SUVmax were determined for the focal uptakes in both pre and post Lasix images. All continuous variables were expressed as mean ± SD. Images were assessed by two experienced physicians in order to evaluate lesion detectability and delineations that have an impact on clinical interpretation. Results:Of total 45 patients, 12/45 (27%) showed unremarkable scan along with 33/45 (73%) showing metastases. Sixteen out of 45 (36%) of the metastatic scans showed indeterminate/equivocal lesions. In these cases, post Lasix study showed false negative findings in 7/45 (16%), better delineation of lesions 10/45 (22%), better confidence towards reporting lesions as abnormal in 5/45 (11%) with an overall 11/45 (24%) of the cases who showed increase in the number of the lesions after the Lasix study. The overall CNR was evaluated using Wilcoxon Rank test (p-value = 0.02) which suggested significant improved ratios in the post-Lasix imaging by 49.6%±24.5. There was a substantial agreement (k =0.76) between the physicians when comparing the lesion clarity and delineation in post Lasix images. The average score for physician one and two being 2.4 ±0.71 and 2.53±0.52 respectively.Conclusion: Post diuretic 68Ga-PSMA imaging at ± 15 minutes clears the unwanted activity in the urinary tract which in turn improves the contrast to noise ratios. Thus leading to decline in false positive findings, improved diagnostic certainty of physician and better detection of indeterminate lesions in 68Ga-PSMA imaging.

Non-invasive somatotransgenic bioimaging in living animals

Bioluminescence imaging enables noninvasive quantification of luciferase reporter gene expression in transgenic tissues of living rodents. Luciferase transgene expression can be regulated by endogenous gene promoters after targeted knock-in of the reporter gene, usually within the first intron of the gene. Even using CRISPR/Cas9 mediated genome editing this can be a time consuming and costly process. The generation of germline transgenic (GLT) rodents by targeted genomic integration of a gene expression cassette in embryonic stem (ES) cells is commonplace but results in the wastage of large numbers of animals during colony generation, back-crossing and maintenance. Using a synthetic/truncated promoter-driven luciferase gene to study promoter activity in a given tissue or organ of a GLT also often results in unwanted background luciferase activity during whole-body bioluminescent imaging as every cell contains the reporter. We have developed somatotransgenic bioimaging; a method to generate tissue-restricted transcription factor activated luciferase reporter (TFAR) cassettes in rodents that substantially reduces the number of animals required for experimentation. Bespoke designed TFARs are delivered to newborn pups using viral vectors targeted to specific organs by tissue-tropic pseudotypes. Retention and proliferation of TFARs is facilitated by stem/progenitor cell transduction and immune tolerance to luciferase due to the naïve neonatal immune system. We have successfully applied both lentiviral and adeno-associated virus (AAV) vectors in longitudinal rodent studies, targeting TFARs to the liver and brain during normal development and in well-established disease models. Development of somatotransgenic animals has broad applicability to non-invasively determine mechanistic insights into homeostatic and disease states and assess toxicology and efficacy testing. Somatotransgenic bioimaging technology is superior to current whole-body, light-emitting transgenic models as it reduces the numbers of animals used by generating only the required number of animals. It is also a refinement over current technologies given the ability to use conscious, unrestrained animals.

Sexual Compliance in Understudied Relationships

ABSTRACT The largely interdisciplinary literature on sexual compliance, or consensual but unwanted sexual activity, primarily focuses on its function and consequence as a gendered relational maintenance activity. The present study investigated if sexual compliance predicts state mental health in addition to relational satisfaction. Data from a sample of understudied relationship types indicates that sexual compliance negatively predicts mental health during the last 30 days and relationship satisfaction in a non-normative relational sample that includes lesbian, gay, bisexual, transgender, and queer (LGBTQ) people, practitioners of bondage, domination, and sadomasochism. (BDSM), and polyamorous individuals. Implications and avenues for future research are discussed.

043 Different Forms of Mental Health Treatments are Associated with Different Patterns of Posttrauma Sexual Functioning in Women: An Exploratory Study

Unwanted sexual activity (USA) and posttraumatic stress disorder (PTSD) have been found to independently predict sexual difficulties. While not all survivors of USA experience significant impairments, those who do have variable prognoses. Treatments for posttraumatic distress can involve pharmacotherapy, psychotherapy, or a combination of both. While certain psychotherapies have been reported to ameliorate sexual problems related to PTSD, many psychiatric medications have negative effects on sexual functioning.

GAMBARAN SEXUAL COMPLIANCE PADA REMAJA PEREMPUAN YANG MELAKUKAN PERILAKU SEKSUAL PRANIKAH

Study about sexual compliance is still rarely done in Indonesia. Study about sexual compliance is also often done with quantitative research methods, so that the results of the study are data percentages that describe the surface of sexual compliance. This study aims to explore sexual compliance in-depth in women who engaged in premarital sexual behavior. This study is a qualitative study with a phenomenological research method. The analysis technique of this study is an inductive thematic analysis. The subjects of this study are two teenage girls who engage in unwanted sexual activity. The results of this study are in the form of themes, which are about premarital sexual behavior, sexual compliance, meaning towards sexual compliance experience and about current condition of the subjects.

English

Sexually transmitted infections pose a public health crisis globally and in low and middle-income countries, and substance use has been linked to an increased risk of engaging in risky sexual behaviors among youths. This work aims to explore the relationship between substance use and risky sexual behaviors among school-based youths in Liberia. An 86-question survey was developed to collect information about substance use and sexual behaviors. The survey was validated using qualitative data obtained from focus groups of Liberian youths. 400 students were sampled with a mean age of 18.15 ±2.14 years. Students who use alcohol were 2.4 times more likely to have multiple sexual partners (OR=2.38, CI= 1.06–5.32, p=0.035), 11 times more likely to engage in unwanted sexual activity (OR=10.86, CI=1.36–86.96, p=0.025); marijuana use increased the risk of multiple unintended pregnancies (OR=5.49, CI= 1.37–22.03, p=0.016); users of heroin had 4 times the odds of engaging in unwanted sexual activity (OR=4.18, CI= 1.07-16.37, p=0.039). Substance use increases the risk of engaging in risky sexual behaviors among youths in Liberia. Intervention programs that target individual, community, and societal level determinants are needed to tackle risky sexual behaviors in this population. Key words: Risky sexual behaviors, substance use, Liberia, youths, adolescents, sexually transmitted infections (STIs).

Effects of deficiency in the RLBP1-encoded visual cycle protein CRALBP on visual dysfunction in humans and mice

Mutations in retinaldehyde-binding protein 1 (RLBP1), encoding the visual cycle protein cellular retinaldehyde-binding protein (CRALBP), cause an autosomal recessive form of retinal degeneration. By binding to 11-cis-retinoid, CRALBP augments the isomerase activity of retinoid isomerohydrolase RPE65 (RPE65) and facilitates 11-cis-retinol oxidation to 11-cis-retinal. CRALBP also maintains the 11-cis configuration and protects against unwanted retinaldehyde activity. Studying a sibling pair that is compound heterozygous for mutations in RLBP1/CRALBP, here we expand the phenotype of affected individuals, elucidate a previously unreported phenotype in RLBP1/CRALBP carriers, and demonstrate consistencies between the affected individuals and Rlbp1/Cralbp−/− mice. In the RLBP1/CRALBP-affected individuals, nonrecordable rod-specific electroretinogram traces were recovered after prolonged dark adaptation. In ultrawide-field fundus images, we observed radially arranged puncta typical of RLBP1/CRALBP-associated disease. Spectral domain-optical coherence tomography (SD-OCT) revealed hyperreflective aberrations within photoreceptor-associated bands. In short-wavelength fundus autofluorescence (SW-AF) images, speckled hyperautofluorescence and mottling indicated macular involvement. In both the affected individuals and their asymptomatic carrier parents, reduced SW-AF intensities, measured as quantitative fundus autofluorescence (qAF), indicated chronic impairment in 11-cis-retinal availability and provided information on mutation severity. Hypertransmission of the SD-OCT signal into the choroid together with decreased near-infrared autofluorescence (NIR-AF) provided evidence for retinal pigment epithelial cell (RPE) involvement. In Rlbp1/Cralbp−/− mice, reduced 11-cis-retinal levels, qAF and NIR-AF intensities, and photoreceptor loss were consistent with the clinical presentation of the affected siblings. These findings indicate that RLBP1 mutations are associated with progressive disease involving RPE atrophy and photoreceptor cell degeneration. In asymptomatic carriers, qAF disclosed previously undetected visual cycle deficiency.

GRNA Sequence Heterology Tolerance Catalyzed by CRISPR/Cas in an In Vitro Homology-Directed Repair Reaction

CRISPR and associated Cas nucleases are genetic engineering tools revolutionizing innovative approaches to cancer and inherited diseases. CRISPR-directed gene editing relies heavily on proper DNA sequence alignment between the guide RNA (gRNA)/CRISPR complex and its genomic target. Accurate hybridization of complementary DNA initiates gene editing in human cells, but inherent gRNA sequence variation that could influence the gene editing reaction has been clearly established among diverse genetic populations. As this technology advances toward clinical implementation, it will be essential to assess what degree of gRNA variation generates unwanted and erroneous CRISPR activity. With the use of a system in which a cell-free extract catalyzes nonhomologous end joining (NHEJ) and homology-directed repair (HDR), it is possible to observe a more representative population of all forms of gene editing outcomes. In this manuscript, we demonstrate CRISPR/Cas complexation at heterologous binding sites that facilitate precise and error-prone HDR. The tolerance of mispairing between the gRNA and target site of the DNA to enable HDR is surprisingly high and greatly influenced by polarity of the donor DNA strand in the reaction. These results suggest that some collateral genomic activity could occur at unintended sites in CRISPR-directed gene editing in human cells.

Associations between sexual precedent and sexual compliance: An event-level examination

Objective Most studies on agreeing to unwanted sex have assessed sexual encounters between people who have had sex before. Thus, we examined instances of sexual compliance with a novel sexual partner. Participants: A probability sample of college students at a university in the Midwest United States (N = 7,112). Methods: Participants completed an online survey based on measures from the National Survey of Sexual Health and Behavior. Results: Only 2.5% (n = 179) agreed to unwanted sexual activity at their most recent sexual encounter. People who were sexually compliant with a novel sexual partner frequently did so due to their own alcohol intoxication. Further, sexual compliance with novel sexual partners was less frequently associated with affectionate sexual behaviors or orgasm. Conclusions: Our initial findings regarding the effect of sexual precedent on sexual compliance warrant further research on instances when people agree to sex they don’t want with novel partners.

A213 A NOVEL PH-SENSITIVE OPIOID ANALGESIC THAT IS SELECTIVELY ACTIVATED IN ACIDIC INFLAMMATORY ENVIRONMENTS

Abstract Background Opioids drugs are effective analgesics for inflammatory disorders such as inflammatory bowel disease (IBD) but their effects at non-inflammed sites can cause serious morbidity and even death. Exploiting the knowledge that tissue pH in inflamed tissues is acidic (e.g. 6.5–7.0), a novel opioid analgesic, ±)-N-(3-fluoro-1-phenethylpiperidine-4-yl)-N-phenylpropionamide (NFEPP), with a low acid dissociation constant, was developed that selectively activates peripheral µ-opioid receptor (MOPr) at acidic pH. Thus, pH-sensitive binding of NFEPP could selectively inhibit nociceptive nerves in the inflamed colon and have no effect on non-inflamed tissues outside the GI tract. Aims Evaluate whether NFEPP causes inhibition of colonic nociceptors at acidic pH’s, which mimic the inflamed colon. Methods To evaluate pH sensitive property of NFEPP to activate MOPr, dorsal root ganglia (DRG) neurons from C57BL/6 mice were exposed to the MOPr agonists NFEPP (300nM, 15 min) or DAMGO (100nM, 15 min) or vehicle at pH 6.5 or 6.8 or 7.4. Neuronal excitability was measured by recording the rheobase (minimum current to fire an action potential) using patch clamp recordings of isolated dorsal root ganglia neurons. In parallel ex vivo studies of mouse colon, extracellular recordings were obtained from afferent nerves innervating the distal colon. Afferent responses to probing with von Frey hair (1 gm) were examined before and after exposure to NFEPP (300nM, 5 min superfusion) at pH 6.5 and 7.4 respectively. Oneway ANOVA and post hoc Dunnett and Bonferroni tests were used to analyze the data. Results In patch clamp studies, NFEPP caused a decrease in DRG excitability at pH 6.5 and 6.8 (increased rheobase 21.3%, p&amp;lt;0.05 and 28.9%, p&amp;lt;0.05 respectively compared to vehicle) but had no effect at physiological pH 7.4. DAMGO, a MOPr agonist, caused inhibition of nociceptor excitability at pH 7.4 (increased rheobase 25.2%, p&amp;lt;0.05 compared to vehicle) as shown in previous experiments, but had no effect at pH 6.5 and 6.8. Vehicle had no effect at the different pH’s. In colonic afferent nerve recordings, NFEPP significantly attenuated afferent response (28.9% P&amp;lt;0.01) to probing at pH 6.5 and this effect was reversed after a 15 min washout. At pH 7.4 NFEPP had no effect on afferent nerve firing. Conclusions NFEPP activated MOPr at acidic pH causing inhibition of colonic nociceptors. This pH-selective agonist provides a new strategy to relieve pain at the site of inflammation while being devoid of any of unwanted activity in non-inflamed organs. Funding Agencies CCC

VLSI implementation of an efficient MBIST architecture using RLFSR

An area and power efficient FPGA implementation approach of memory built-in self-test (MBIST) is presented in this paper. It is consist of an array of 2-bit linear feedback shift register (LFSR), the switching activity of address generator used in previous MBIST architectures are high. This unwanted switching activity is affecting the power consumption of entire MBIST. We propose the MBIST with ring-based LFSR used to avoid the main problem of power consumption. The 2-bit 2N pattern generator combines with a conventional (N − 2) bit 2(N−2) LFSR and a 2-bit four pattern generator are both controlled separately by a two separate clocks of two different frequencies to generate all possible memory address location of a memory under test. The proposed architecture is implemented on vertex4 FPGA technology in Xilinx software. We showed that results improve the performance of the proposed design by comparing with the existing design.