Ceramides and glycosphingolipids (GSLs) are minor components in most eukaryotic cells. Since ceramides may be generated in lipid raft like domains by enzyme degradation of sphingomyelin (SM), ceramide/GSL interactions may become relevant in cell membranes. To examine their mutual interactions, we have prepared binary and ternary model bilayer systems composed of a disordered lipid (unsaturated phosphatidylcholine), and different combinations of saturated sphingolipids (palmitoyl SM, palmitoyl ceramide (PCer), and hydroxylated or non-hydroxylated galactosyl or glucosyl palmitoyl-ceramide (PGalCer or PGlcCer)). We have used trans-parinaric acid (tPA) as a probe to detect the ordered domains formed by the sphingolipids in the phosphatidylcholine bilayer. In binary systems, the PCer formed the most thermostable ordered domains, followed by PGalCer, OH-PGalCer, OH-PGlcCer, and PGlcCer. The PSM domains were the least thermostable. Addition of PCer to the GSL or PSM domains increased their thermostability, with the exception of PGalCer, whose thermostability was unaffected by inclusion of PCer. Lifetime analysis of tPA suggested that all sphingolipid ordered domains became even more ordered in the presence of PCer. We conclude that PCer was able to interact with all the examined sphingolipids and increased packing order in the domains.