Unsaturated Fatty Acid Content Research Articles

Effect of cinnamon powder on quality attributes and off-flavor in fried chicken drumsticks made from long-term thawed Korean native chicken

The effect of cinnamon powder on the quality and mitigation of off-flavor in fried chicken drumsticks made from long-term thawed Korean native chicken (Woorimatdag No. 1, WRMD1) was investigated. The WRMD1 drumsticks were categorized into 5 groups: conventional thawing (16 h, CT), long-term thawing (48 h, LT), cinnamon powder added into 'LT' as marinade (0.03%, CM) or incorporated into the batter (1.35%, CB), and long-term thawing with cinnamon powder incorporated both in the marinade and batter (0.03% + 1.35%, CMB). The crude fat content was significantly higher in the CT and CMB than that of the CB. The CM, CB, and CMB showed significantly lower levels of 2-thiobarbituric acid reactive substance compared with the CT and LT. The predominant fatty acids in all treatments were C18:1n9, C18:2n6, and C16:0. The LT displayed lower total unsaturated fatty acid content than the CT (P < 0.05). The CM effectively decreased lipid oxidative volatiles, such as 1-octanol, 1-octen-3-ol, and 2-octen-1-ol, (E), in the LT (P < 0.05). Both the CM and CB showed an inclination to increase specific pyrazines associated with pleasant notes compared with the LT, and showed higher levels of pyrazines, such as pyrazine, 2-ethyl-6-methyl-, and pyrazine, 3-ethyl-2,5-dimethyl-, than those of the CMB (P < 0.05). The CM contained higher levels of 2,3-butanedione when compared with the other groups (P < 0.05). Multivariate analysis demonstrated that cinnamon had an effect in discriminating the treatment groups with cinnamon addition from both the CT and LT, whereas the CM, CB, and CMB formed distinct clusters. The CM and CMB received significantly higher aroma scores from panelists in comparison to the other groups. These findings suggest that the CM (0.03% cinnamon powder) can be used to enhance the aroma in fried WRMD1 drumsticks by reducing or masking the off-flavor volatiles associated with long-term thawing.

Behavioral toxicity of TDCPP in marine zooplankton: Evidence from feeding and swimming responses, molecular dynamics and metabolomics of rotifers

As new organic flame retardants, chlorinated organophosphate esters (Cl-OPEs) have high water solubility and structural similarity to organophosphate pesticides, posing risks to aquatic organisms. The potential neurotoxicity of Cl-OPEs has attracted attention, especially in marine invertebrates with a relatively simple nervous system. In this study, a marine rotifer with a cerebral ganglion, Brachionus plicatilis, was exposed to tris (1,3-dichloro-2-propyl) phosphate (TDCPP) (two environmental concentrations and one extreme level), and the changes in feeding and swimming behaviors and internal mechanism were explored. Exposure to 1.05 nM TDCPP did not change the filtration and ingestion rates of rotifers and average linear velocity. But 0.42 and 4.20 μM TDCPP inhibited these three parameters and reduced unsaturated fatty acid content, reproduction and population growth. All TDCPP test concentrations suppressed AChE activity, causing excessive accumulation of acetylcholine within rotifers, thereby disturbing the neural innervation of corona cilia. Molecular docking and molecular dynamics revealed that this inhibition was because TDCPP can bind to the catalytic active site of rotifer AChE through van der Waals forces and electrostatic interactions. TRP420 was the leading amino residue in the binding, and GLY207 contributed to a hydrogen bond. Nontargeted metabolomics using LC-MS and GC-MS identified differentially expressed metabolites in TDCPP treatments, mainly from lipid and lipid-like molecules, especially sphingolipids. TDCPP decreased ganglioside content but stimulated ceramide generation and the expression levels of 3 genes related to ceramide de novo synthesis. The mitochondrial membrane potential (MMP) and ATP content decreased, and the electron respiratory chain complex and TCA cycle were deactivated. An inhibitor of ceramide synthase, fumonisin, alleviated MMP and ATP, implying a critical role of ceramide in mitochondrial dysfunction. Thus, TDCPP exposure caused an energy supply deficit affecting ciliary movement and ultimately inhibiting rotifer behaviors. Overall, this study promotes the understanding of the neurotoxicity of Cl-OPEs in marine invertebrates.

Biochar as sustainable input for nodulation, yield and quality of mung bean

To reduce dependency on synthetic fertilizers in response to the escalating costs of fertilizers and environmental limitations, it is imperative to enhance crop productivity and soil fertility sustainably. This research was carried out at the Agricultural Research Farm of Abdul Wali Khan University in Mardan, Pakistan, with the objective of investigating the effects of biochar on the qualitative attributes of mung beans. The randomize complete block design (RCBD) was used for the experiment having four replication. The treatments comprised of four levels of biochar i.e. 0, 10, 20 and 30 t ha−1. Our results revealed that increasing biochar content caused an increase in yield components as well as attribute composition. The nodule density (17.8), pods plant−1 (27.3), grains pods−1 (11.4) and biological yield (6497 kg ha−1) produced best results under the application of 30 t ha−1 of biochar. Moreover, grain yield (1550 kg ha−1), grain nitrogen content (25.2 g kg−1) and straw nitrogen content (15.3 g kg−1) also resulted best under 30 t ha−1 biochar. While, 1000 grain weight (64 g) was recorded highest weight under 20 t ha−1. The quality attributes showed that the oil content (41.1%), as well as the saturated and unsaturated fatty acid contents (13.7%), were found to be the best under the application of 30 t ha−1. While, protein (23.37%) and linoleic acid (23.128%) content were the highest at 10 t ha−1of biochar. Moreover, the palmitic acid (6.1025%) and stearic acid (2.64%) content resulted higher under 20 t ha−1 of biochar. All the attributes showed positive response to either small level of biochar or a large level, but their response showed that biochar can be a factor that improves both yield and quality. The study therefore suggests that biochar should be applied to the soil to improve its fertility in regards of nutrient and increased organic matter.

Effects of Dietary Aflatoxin B1 on Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) Growth, Intestinal Health, and Muscle Quality.

This study investigated the effects of varying doses of dietary aflatoxin B1 (AFB1) on the growth, intestinal health, and muscle quality of hybrid grouper. Four diets with varying AFB1 concentrations (0, 30, 445, and 2,230 μg kg-1) were used. Elevating AFB1 concentrations led to a decline in growth indexes, specifically the weight gain rate and the specific growth rate, although the survival rate remained unchanged. Morphological indicators showed a dose-dependent decline with AFB1 exposure. Intestinal MDA content and hindgut reactive oxygen species (ROS) levels increased, while antioxidant indexes and digestive enzymes decreased with higher AFB1 levels. AFB1 negatively influenced hindgut tight junction protein and antioxidant-related gene expression while promoting inflammation-related gene expression. The presence of AFB1 in the experiment led to a decrease in beneficial intestinal bacteria, such as Prevotella, and an increase in harmful intestinal bacteria, such as Prevotellaceae_NK3B31_group. Muscle lipid and unsaturated fatty acid content significantly decreased, while muscle protein and liver AFB1 content increased dramatically with higher AFB1 concentrations. AFB1 caused myofibrillar cleavage and myofilament damage, leading to increased spaces between muscle fibers. In conclusion, diets with AFB1 levels exceeding 30 μg kg-1 inhibited hybrid grouper growth, while levels surpassing 445 μg kg-1 resulted in hindgut ROS accumulation, inflammation, elevated intestinal permeability, reduced digestive enzyme activity, and compromised muscle quality.

Aqueous enzymatic extraction of macauba (Acrocomia aculeata) pulp oil: A green and sustainable approach for high-quality oil production

Aqueous enzymatic extraction (AEE) of macauba pulp oil (MPO) was performed in this study with five commercial enzymatic pools. The chemical, nutritional, and thermal properties of the oils with high oil efficiency by AEE were evaluated and compared with mechanical pressing (MP) and organic solvent extraction (SE). Among the AEE processes, the pectinase pool (at pH 5.5 and 50 °C) exhibited the highest process efficiency (88.6 %). The oils presented low acidity values (0.4–3.1 %) and low molar absorptivities, indicating minimal oil degradation. Bioactive compounds, such as carotenoids, were found in MPO. The iodine index and the fatty acid profile of the oils revealed a high content of unsaturated fatty acids, particularly oleic and linoleic acids, with excellent nutritional scores, as evidenced by anti-atherogenicity and anti-thrombogenicity indices. These findings emphasized that AEE is an eco-friendly approach for extracting high-quality MPO with beneficial health compounds for food products.

Herbage accumulation, nutritive value, and fatty acid profile of bermudagrass cultivars grown in different latitudes

AbstractThe geographical distribution of bermudagrass cultivars is limited by freezing events (&lt;0°C), commonly associated with higher latitudes in the northern hemisphere. The lipid composition in the plant cell membranes is critical to promote persistence under freezing conditions. This study evaluated herbage accumulation (HA), nutritive value, and lipid biosynthesis of different bermudagrass cultivars in Ona, FL (27°26' N 82°55' W) and Marianna, FL (30o52' N 85o11' W), in 2 years. Treatments were five bermudagrass cultivars (Coastal, Tifton 44, Tifton 85, Jiggs, and Mislevy) distributed in a randomized complete block design with four replicates. HA was greater in Marianna than in Ona for all cultivars (p &lt; 0.05). Mislevy and Jiggs had greater HA in Marianna than the others (p &lt; 0.05), while all cultivars were similar in Ona (p &gt; 0.05). There was no difference between locations or among the cultivars for crude protein and neutral detergent fibre, but bermudagrass cultivars had greater in vitro digestibility of organic matter (IVDOM) in Marianna than in Ona (p &lt; 0.05). The concentration of unsaturated fatty acids increased in the northern latitude (Marianna; p &lt; 0.05). Coastal had greater unsaturated fatty acid concentration than other cultivars (p &lt; 0.05). Geographical location affects HA, IVDOM, and unsaturated fatty acid concentration of bermudagrass cultivars. The unsaturated fatty acid concentration plays a key role in acclimating bermudagrass to different thermal environments. Coastal showed great potential to tolerate chilling stress in Florida. Further studies may be developed to identify promising molecular markers for cold tolerance in bermudagrass.

Food waste upcycling via microalgal adaptive evolution for lipids and fatty acids enhancement towards circular bioeconomy

Disposal of food waste (FW) has become a significant problem that exacerbates the economy, environment and climate due to its rich nutrient content, thereby implying the necessity to upcycle FW by biotechnology means in a sustainable manner. Employing photosynthetic microalgae to FW upcycling hold great potential to simultaneously remediate the FW and to biosynthesize novel commodity metabolites. However, effective nutrient utilization of FW and metabolite overproduction by native algae is inadequate, and thus further development of strain and strategy is warranted to achieve a circular bioeconomy. Here, the marine Nannochloropsis oceanica was improved by adaptive laboratory evolution (ALE) under low salt conditions for enhanced lipid and uncompromised growth features. Then, FW hydrolysate (FWH) was used as the carbon source to facilitate the mixotrophic cultivation of N. oceanica. Results showed that the biomass of scrutinized evolved cells (ALE70) resulted in enhanced biomass production of 830 mg/l using FWH. Interestingly, ALE70 strains accumulate lipids up to 49 % of total cell dry weight, which was 1.5-fold higher than the WT cells cultivated in FWH. Importantly, FAME analysis by GC–MS showed that FWH provision to the ALE70 strains resulted in notable fatty acid alteration, enriched explicitly with unsaturated fatty acid and EPA content was hiked by 32 % of FAME. Altogether, these findings demonstrated a potential duplex strategy by employing the evolved strains to upcycle the food waste for valuable metabolite biosynthesis effectively and also offer promising insights into algal-associated food waste valorization towards circular bioeconomy.

Biometry, Growth, and Recruitment Pattern of a Commercially Important Nereid polychaete, Namalycastis fauveli, from the East Coast of Bangladesh

Certain benthic polychaetes, such as species within the genus Namalycastis, are employed in the diet of gravid shrimps in aquaculture due to their amino acids and highly unsaturated fatty acid content, enhancing the quality of gravid shrimp. Despite its importance in the rapidly developing shrimp culture industry, the population parameters of this economically valuable species are unknown. Therefore, the present study examines the population parameters of Namalycastis fauveli to assess its occurrence, growth, recruitment, exploitation level, and stock status in Bangladesh. Monthly samplings of N. fauveli and environmental variables were collected from five sites of the Cox’s Bazar coast using a square-shaped mud corer with a 0.093 m2 (or 1.0 ft2) mouth opening from August 2020 to July 2021 to measure or estimate. Within the 8.0–30.0 cm depth range of the intertidal zone, polychaete samples were collected from the sediment. The results showed negative allometric growth (b &lt; 3), but there was a significant L–W relationship (p &lt; 0.05, r2 = 0.43 to 0.94). The estimated L∝, K, and ϕ were 22.05 cm, 0.99 year−1, and 2.69, respectively, while total mortality (Z) was 4.56 year−1. It was calculated that the fishing mortality and capture probability proportionally increased with the total length at a certain age. Recruitment mostly occurred in October and March, and temperature had a greater impact than salinity. The evaluated exploitation level (E = 0.57) indicated that the stock was overexploited. Thus, the above results provide some valuable information for shrimp farmers and stakeholders, as well as for policymakers in the move towards restoration, species conservation, and efficient management of N. fauveli natural stocks.

Effect of Different Edible Trichosanthes Germplasm on Its Seed Oil to Enhance Antioxidant and Anti-Aging Activity in Caenorhabditis elegans

The seeds of various Trichosanthes L. plants have been frequently used as snacks instead of for traditional medicinal purposes in China. However, there is still a need to identify the species based on seeds from Trichosanthes germplasm for the potential biological activities of their seed oil. In this study, 18 edible Trichosanthes germplasm from three species were identified and distinguished at a species level using a combination of seed morphological and microscopic characteristics and nrDNA-ITS sequences. Seed oil from the edible Trichosanthes germplasm significantly enhanced oxidative stress tolerance, extended lifespan, delayed aging, and improved healthspan in Caenorhabditis elegans. The antioxidant activity of the seed oil exhibits a significant positive correlation with its total unsaturated fatty acid content among the 18 edible Trichosanthes germplasm, suggesting a genetic basis for this trait. The biological activities of seed oil varied among species, with T. kirilowii Maxim. and T. rosthornii Harms showing stronger effects than T. laceribractea Hayata.

Rumen degradable protein levels on fatty acid profile of rumen bacteria and milk fat of dairy cows supplemented with calcium salts of fatty acids.

This study aimed to evaluate the effects of calcium salts of fatty acids (CSFA) from soybean oil in diets with different levels of rumen degradable protein (RDP) on bio-hydrogenation extent, and fatty acid (FA) profile intake, omasal digesta, rumen bacteria, and milk fat. Eight Holstein lactating cows were used in a replicated 4 × 4 Latin square design. Treatments were arranged in a 2 × 2 factorial design with two CSFA levels (0 or 33.2g/kg DM of CSFA) and two RDP levels (98.0 or 110 g/kg DM). There was RDP and CSFA interaction effect on the omasal flow of total FA and some specific FA. Only in -CSFA diets, the higher RDP level increased omasal flow of total FA. Dietary RDP levels did not affect the FA profile of bacteria and milk fat. Feeding CSFA reduced or tended to reduce the bacterial proportion of C15:0, C16:0, C16:1, C17:0, and C18:0 FA and decreased the concentrations of short- and medium-chain FA (<18C) and increased the concentrations of unsaturated and long-chain FA (≥18C) in milk fat. Feeding CSFA of soybean oil increases the intake and omasal flow of FA and augments unsaturated FA content in bacteria pellets and milk fat.

Combined of plasma-activated water and dielectric barrier discharge atmospheric cold plasma treatment improves the characteristic flavor of Asian sea bass (Lates calcarifer) through facilitating lipid oxidation

To explore the combination effects of plasma-activated water and dielectric barrier discharge (PAW-DBD) cold plasma treatment on the formation of volatile flavor and lipid oxidation in Asian sea bass (ASB), the volatile flavor compounds and lipid profiles were characterized by gas chromatography-ion mobility spectrometry and LC-MS-based lipidomics analyses. In total, 38 volatile flavor compound types were identified, and the PAW-DBD group showed the most kinds of volatile components with a significant (p < 0.05) higher content in aldehydes, ketones, and alcohols. A total of 1500 lipids was detected in lipidomics analysis, phosphatidylcholine was the most followed by triglyceride. The total saturated fatty acids content in PAW-DBD group increased by 105.02 μg/g, while the total content of unsaturated fatty acids decreased by 275.36 μg/g. It can be concluded that the PAW-DBD processing increased both the types and amounts of the volatile flavor in ASB and promoted lipid oxidation by altering lipid profiles.

Analysis of genes specific to the early maturation stage of Sesamum indicum seeds by subtraction method*,*.

The mechanisms regulating the content ratio of unsaturated fatty acid in sesame oil need to be clarified in order to breed novel varieties with high contents of unsaturated fatty acids. Full-length cDNA libraries prepared from sesame seeds 1 to 3 weeks after flowering were subtracted with cDNAs from plantlets of 4 weeks after germination. A total of 1545 cDNA clones was sequenced. The functions of novel genes expressed specifically during the early maturation of sesame seeds were investigated by the transformation of Arabidopsis thaliana. Thirteen genes for a transcription factor were identified, four of which were involved in ethylene signaling. Fifty-nine genes, including those for the aquaporin-like protein and ethylene response factor, were analyzed by overexpression in A. thaliana. The overexpression of novel genes and the aquaporin-like protein gene in A. thaliana increased the content of unsaturated fatty acids. The localization of these products was investigated by the induction of the expression vectors for the GFP fusion protein into onion epidermal cells and sesame root cells with a particle gun. As a result, two cDNA clones were identified as good candidate genes to clarify the regulation in the yield and the ratio of unsaturated fatty acids in sesame seeds. Sein60414 (Accession No. LC603128), an intrinsic membrane protein, may be involved in the increase of unsaturated fatty acids, and Sein61074 (Accession No. LC709278) MAP3K δ-1 protein kinase in the regulation of the total ratio of unsaturated fatty acids in sesame seeds.

Quality assessment of flax advanced breeding lines varying in seed coat color and their potential use in the food and industrial applications

BackgroundWith the increasing consumer awareness of the strong relationship between food and health, flax became a promising functional food due to its bioactive nutraceutical composition. Intra-specific crosses of eight contrasting flax genotypes were performed previously, and within segregating F6 progeny families, we investigated a close-up composition of phytochemicals derived from whole seeds.ResultsThe considerable genetic variation among the flax F6 families suggested that intra-specific hybridization is essential in flax breeding to obtain and broaden genetic variability and largely affirmed the opportunity for selecting promising lines. Also, significant variations in the targeted metabolite contents and antioxidant properties were observed among brown and yellow-seeded families. Notably, brown-seeded families expressed the highest average values of saturated fatty acids, protein, fiber, tocopherol, phenolics, SDG, and SECO lignans. Yellow-seeded families represented the highest average content of unsaturated fatty acids and mucilage. The cultivation year significantly affects flaxseed’s composition and functional properties, presumably due to temperature, humidity, and sunshine time differences. Interestingly, the seeds obtained in warmer conditions were more potent and had more chemical constituents. The favorable genetic correlations among all evaluated traits suggest the possibility of joint genetic selection for several nutritional and phytochemical characteristics in flax. The current study highlights the importance and utilization of 19 top families as their seeds and oil play imperative roles in the pharmaceuticals and food industries. The antioxidant capacity of the seeds showed that families 84B, 23B, 35Y, 95Y, 30B, 88B, and 78B serve as a natural source of dietary antioxidants beneficial to human health. To increase the oxidative stability of the flaxseed oil, the quality evaluation identified some families with low levels of linolenic acid.ConclusionsThese findings are essential to improving flaxseed’s nutritional quality and therapeutic properties through a bulk breeding program.

Effect of high DHA and ARA fortification on lipid oxidation of infant formula powder

ABSTRACT Our objective was to assess the impact of fortified high-dose docosahexaenoic acid (DHA) and arachidonic acid (ARA) in infant formula powders (IFs) on stability. We compared and evaluated the stability of IFs containing high and low doses of DHA and ARA over a 6-month storage period at 37°C. While most formulations showed minimal changes in unsaturated fatty acid content of during storage, significant losses of DHA and ARA were observed in some high-dose formulations. Furthermore, this study noted consistent trends in the changes of vitamin C and vitamin E across both formula groups. Notably, the high-dose group formulas exhibited a higher presence of volatile organic compounds (VOCs) during storage, along with a more pronounced increase in thiobarbituric acid-reactive substance values (TBARS). In conclusion, the inclusion of high doses of DHA and ARA in IFs may compromise their oxidative stability.

Variability of the Main Economically Valuable Characteristics of Cyperus esculentus L. in Various Ecological and Geographical Conditions.

This study includes an assessment of the VIR (Center N.I. Vavilov All-Russian Institute of Plant Genetic Resources) chufa collection, grown in various ecological and geographical conditions of the Russian Federation: "Yekaterininskaya experimental station VIR" in the Tambov region and "Kuban experimental station VIR" in the Krasnodar Region during the years 2020-2021. The main indicators of the economic value of chufa accessions were studied: yield structure and nutritional value (oil, protein, starch, and fatty acid profile). The accessions were grown in regions with different climatic conditions. As a result of the study, the variability of the biochemical and yield characteristics and the correlation between the studied indicators and the factor structure of its variability were established. Of the 20 accessions used in the study, the accessions with the highest protein, starch, oil and unsaturated fatty acid contents were selected, which are the most promising for their use as a raw material to expand the range of regional functional food products, as well as for future breeding efforts in the development of new, promising regional chufa varieties.

Lipidomic analysis of facial skin surface lipids in acne in young women.

Alterations in the secretion and composition of skin surface lipids (SSL) are closely associated with the development of acne. Lipidomics is a useful tool to analyse the SSL of different types of acne. Our previous study found that phosphatidylserine and triacylglycerols dominate SSL changes in male acne and infantile acne, respectively. However, skin surface lipids as well as specific lipids in female acne patients remain to be investigated. To analyse and compare the SSL profile of acne women and healthy women and to discuss the involvement of differential lipids in acne development. Systematic lipidomics approach (high-throughput UPLC-QTOF-MS technology in combination with multivariate data analysis methods) was used to analyse the variations of SSL between acne and healthy groups. Analysis revealed significant differences in lipid content and composition between the two groups. Further analysis showed that levels of 13 individual lipids were significantly different and followed the same trend as the main class and subclasses. The largest individual contributor to the subgroup was triglycerides (TG) and phosphatidylinositol (PI). In addition, female acne patients exhibited reduced ceramide chain length (CCL) and increased levels of unsaturated fatty acids (UFAs), The changes of CCL in female acne are identical to male acne. There was a significantly higher level of TG and PI in the SSL of female acne patients. A reduction in CCL and an increase in UFAs content might contribute to the reduced skin barrier function in acne patients. The results suggest that female acne may have different pathogenesis than male acne.

Rationale for obtaining protein concentrate of industrial hemp seeds of Cannabis sativa L. of modern selection based on mathematical modeling and its application in food production

In recent decades interest in hemp seed concentrates, which are characterized by a valuable amino acid composition and high nutritional value, has increased. Hemp seeds are sources of vegetable protein and essential components for maintaining health due to the rich content of protein compounds, vitamins, and unsaturated fatty acids. So, production of protein concentrates from oilseeds is becoming relevant. Taking this into account, it is relevant to conduct research aimed at developing and improving the technology for obtaining protein products from the seeds of industrial hemp Cannabis Sativa L of modern selection, as well as expanding the possibility of their use as a part of a different range of food products. The seeds of industrial hemp Cannabis Sativa L of modern selection were used as objects of the research. The studies used both generally accepted and special chemical, physicochemical and physical methods for studying the quality of raw materials and finished products. The research was carried out to improve the technology for obtaining protein concentrate from the seeds of industrial hemp Cannabis Sativa L. based on mathematical modeling of the process, which showed the greatest efficiency in extracting protein concentrate using a 20% alcohol solution as an extractant. Analysis of the composition of the resulting protein concentrate showed a predominant content of globulins. At the same time, the composition of globulin fractions, in comparison with albumin, indicates a great potential for the use of globulin in the formulations of functional food products, as well as a component of targeted technological action. In general, the studies have shown the feasibility of using protein concentrates from industrial hemp seeds of Cannabis Sativa L of modern selection in food products as a functional and technological ingredient.

Milk fortified with chitooligosaccharide–gallic acid conjugate and its liposome: characterisation, storage stability, and in vitro digestibility

SummaryThe effects of chitooligosaccharide–gallic acid conjugate (CGC) and its liposome (CGLP) at two levels (1.25 and 2.50%, w/v) on quality and shelf‐life of milk kept at 4 °C were studied. Microbial counts including total viable, pathogenic and spoilage bacteria were the lowest for CGC‐fortified milk, especially when 2.50% (w/v) was used (CGC‐2.50) than remaining samples (P &lt; 0.05). Lower thiobarbituric acid reactive substances and peroxide values were obtained for CGC‐2.50 than other samples (P &lt; 0.05). However, higher acidity values and decreased pH and lightness were noticed in the aforementioned sample (P &lt; 0.05). Moreover, all fortified milk samples had higher bioactivities during in vitro digestion, in which CGC‐2.50 had the highest bioactivities (P &lt; 0.05). Higher retention of unsaturated fatty acids content in CGC‐2.50 was obtained than other counterparts (P &lt; 0.05). Therefore, CGC efficiently extended milk shelf‐life with high acceptability at 4 °C for a minimum of 6 days, whereas control had a shelf‐life of less than 4 days.

Optimization of soybean oil content in a soybean oil-based aquafeed for grass carp (Ctenopharyngodon idella) to achieve optimal growth performance, proximate and fatty acid compositions, and lipid metabolism

Soybean oil (SO), an important plant-derived lipid, is routinely used in aquafeed as a partial substitute for fish oil. However, the effect of various contents of SO in a SO-based aquafeed on specific aquatic animals and the underlying mechanisms are unclear. In this study, five isonitrogenous diets containing different SO contents (0 %, 3 %, 6 %, 9 %, and 12 %, named as S0, S3, S6, S9, and S12, respectively) were prepared with SO as the predominant lipid source, and 12-week feeding experiments were conducted on juvenile grass carp (Ctenopharyngodon idella) (initial weight: 12.54 ± 0.08 g). Apart from lower feed conversion ratio in the S6 and S9 groups (P < 0.05), no obvious changes were observed in other growth indices (e.g., final weight and weight gain rate) in grass carp fed on diets with 0–9 % SO (P > 0.05). The S12 group exhibited significant decrease in the final weight and weight gain rate and increase in the viscerosomatic index (P < 0.05). Unlike gradual decrease in moisture, ash, and saturated fatty acid contents, muscle crude fat and unsaturated fatty acid (particularly polyunsaturated fatty acid) contents significantly increased with the increasing SO contents (P < 0.05). Serum triglyceride contents continuously increased with the increasing SO amounts, exhibiting the maximum value in the S12 group (P < 0.05). Similarly, the S12 group displayed the highest levels of aspartate aminotransferase, alanine transaminase, low-density lipoprotein cholesterol, and total cholesterol in serum (P < 0.05). Only the S12 group exhibited scanty lymphocytic infiltration in some areas of the hepatopancreas. However, other groups exhibited no obvious histopathological abnormalities. Lipogenic genes (dgat, fad, elovl1, elovl6, elovl7, lpcat, and pla2), ACSL family members (acsl1a, acsl1b, acsl3, acsl4, acsl5, and acsl6), lipolytic genes (atg1, cpt1a, cpt1b, hsl, and lpl), fatty acid transport-related genes (cd36, fabp, and fatp), and transcription factor involved in lipid metabolism (ppar α) were gradually upregulated as dietary SO content increased (P < 0.05). However, the transcript levels of srebp1 and its target gene acc1 exhibited decreasing trend under the same conditions, and significant downregulation of ppar γ was observed in the S12 group (P < 0.05). In conclusion, the optimal content of SO as the main lipid source in a SO-based diet for juvenile grass carp ranged from 6 % to 9 %, at which growth requirements of grass carp were met and fish health and fillet quality were effectively maintained. Our data provided a scientific reference for developing more efficient aquafeed formulations and enhancing fish flesh quality in aquaculture, as well as provided basic information for further assessing the regulatory mechanism of lipid homeostasis in teleost fishes.

Anti-diabetic, antiglycant, antioxidant, and anticancer activities of Crinum americanum L. leaves lipid fraction

The commercialization of medicinal plants, as recognized in their uses by the nutraceutical, cosmeceutical, and pharmaceutical industries, is gaining more popularity and interest around the globe. The plant family Amaryllidaceae has a long history in the traditional medicinal system and is used to treat diverse diseases. In Brazil, the Crinum americanum L. has been applied by riverside communities for cancer treatment. In this study, the lipid fractions (LF) of leaves of C. americanum L., obtained by Soxhlet (SX) and ultrasonic bath (UAE), were characterized by gas chromatography–mass spectrometry (GC-MS) and evaluated for their anti-diabetic properties, antiglycante, antioxidant, and cytotoxic potential. The extraction yield was higher for UAE (9.45%) than SX (5.99%). Furthermore, the LF obtained by UEA had a higher content of unsaturated fatty acids (73.34%) when compared to the SX (48.94%). However, compounds extracted by both methods showed in vitro anti-diabetic, antioxidant, and anticancer activity against a lung cancer cell line. These results demonstrate that UAE can be used as an efficient and important method in relation to the SX, due to the preservation of the functional properties, reduction of time, and amount of solvent used to extract fatty acids.