Unknown Agents Research Articles

Secure Consensus Control of Multiagent Cyber-Physical Systems With Uncertain Nonlinear Models

Achieving consensus over a class of multiagent systems (MASs) under cyberattacks is studied in this article. The existing literature on secure consensus control of under-attack MASs is based on linear properties of agents models, whereas in practice, linearization may not be feasible in the presence of model uncertainties. Based on this motivation, the main contribution of this article is secure consensus control of MASs in the presences of uncertain nonlinearities in agents models. An MAS is considered consisting of a set of normal agents and a set of attacked malicious agents. A criterion is developed under which each normal agent at each time instant selects safer interaction links to avoid divergence from a consensus/agreement state in the presence of the unknown malicious agents. Accordingly, a network of nonlinear robust controllers is proposed such that under the selection criterion and in the presence of uncertain nonlinearities in the agents models, consensus among the normal agents is guaranteed. Numerical examples validate the accuracy of the proposed consensus control scheme.

The toxicity of the methylimidazolium ionic liquids, with a focus on M8OI and hepatic effects

Ionic liquids are a diverse range of charged chemicals with low volatility and often liquids at ambient temperatures. This characteristic has in part lead to them being considered environmentally-friendly replacements for existing volatile solvents. However, methylimidazolium ionic liquids are slow to break down in the environment and a recent study at Newcastle detected 1 octyl 3 methylimidazolium (M8OI) – an 8 carbon variant methylimidazolium ionic liquid - in soils in close proximity to a landfill site. The current M8OI toxicity database in cultured mammalian cells, in experimental animal studies and in model indicators of environmental impact are reviewed. Selected analytical data from the Newcastle study suggest the soils in close proximity to the landfill site, an urban soil lacking overt contamination, had variable levels of M8OI. The potential for M8OI - or a structurally related ionic liquid – to trigger primary biliary cholangitis (PBC), an autoimmune liver disease thought to be triggered by an unknown agent(s) in the environment, is reviewed.

On the interpretation of the atmospheric mechanism transporting the environmental trigger of Kawasaki Disease.

Recent advances on the environmental determinants of Kawasaki Disease have pointed to the important role of the atmospheric transport of a still unknown agent potentially triggering the disease. The hypothesis arose from an innovative methodology combining expertise in climate dynamics, the analysis of ocean and atmosphere data, the use of dispersion models and the search for biological agents in air samples. The approach offered a new perspective to reveal the identity of the potential trigger, but at the same time, it increased the level of complexity, which could potentially lead to the misinterpretation of the mechanisms. Some years after it was originally formulated, we here provide a brief clarification on the approach and limits of the methodology in order to prevent an eventual misuse of our research ideas and theory, so that further research can better focus on the knowledge gaps that still remain open.

Monitoring Silent Spillovers Before Emergence: A Pilot Study at the Tick/Human Interface in Thailand.

Emerging zoonoses caused by previously unknown agents are one of the most important challenges for human health because of their inherent inability to be predictable, conversely to emergences caused by previously known agents that could be targeted by routine surveillance programs. Emerging zoonotic infections either originate from increasing contacts between wildlife and human populations, or from the geographical expansion of hematophagous arthropods that act as vectors, this latter being more capable to impact large-scale human populations. While characterizing the viral communities from candidate vectors in high-risk geographical areas is a necessary initial step, the need to identify which viruses are able to spill over and those restricted to their hosts has recently emerged. We hypothesized that currently unknown tick-borne arboviruses could silently circulate in specific biotopes where mammals are highly exposed to tick bites, and implemented a strategy that combined high-throughput sequencing with broad-range serological techniques to both identify novel arboviruses and tick-specific viruses in a ticks/mammals interface in Thailand. The virome of Thai ticks belonging to the Rhipicephalus, Amblyomma, Dermacentor, Hyalomma, and Haemaphysalis genera identified numerous viruses, among which several viruses could be candidates for future emergence as regards to their phylogenetic relatedness with known tick-borne arboviruses. Luciferase immunoprecipitation system targeting external viral proteins of viruses identified among the Orthomyxoviridae, Phenuiviridae, Flaviviridae, Rhabdoviridae, and Chuviridae families was used to screen human and cattle Thai populations highly exposed to tick bites. Although no positive serum was detected for any of the six viruses selected, suggesting that these viruses are not infecting these vertebrates, or at very low prevalence (upper estimate 0.017% and 0.047% in humans and cattle, respectively), the virome of Thai ticks presents an extremely rich viral diversity, among which novel tick-borne arboviruses are probably hidden and could pose a public health concern if they emerge. The strategy developed in this pilot study, starting from the inventory of viral communities of hematophagous arthropods to end by the identification of viruses able (or likely unable) to infect vertebrates, is the first step in the prediction of putative new emergences and could easily be transposed to other reservoirs/vectors/susceptible hosts interfaces.

Damage to artificial seedlings across a disturbed Afromontane forest landscape

AbstractSeedling mortality is an important demographic bottleneck for forest regeneration, yet the factors influencing recruitment are often poorly characterized across space and time. In African highlands, where extensive patches of previously disturbed forests remain in persistent shrub‐dominated states, patterns of damage to seedlings have not been examined in detail. We used artificial seedlings to determine how (non‐herbivory‐related) damage varies across a fragmented forest landscape in the Bwindi Impenetrable National Park, Uganda. A total of 848 artificial seedlings were established in 106 plots, each with 8 seedlings, across a gradient of environmental factors, including habitat type, topographic position, distance to water, and distance to human activity. We divided damage into that due to vertebrates, plant debris, and unknown agents. From our 16,960 monthly stem assessments, 1,289 damage events were recorded. The mean “seedling” damage per year (± 1 SE) was 59.5 ± 2.3%. Damage was significantly more frequent in sites dominated by short herbs (66.1 ± 3.0) than in sites dominated by shrubs (60.1 ± 2.6) and ferns (47.9 ± 2.7). The mean percentage of damage by each class of agents was 45.8 ± 2.1% for vertebrates, 21.4 ± 2.1% for plant debris, and 3.8 ± 0.6% for unknown agents. These rates surpass those reported in other montane forests, likely reflecting the density of large vertebrates in our site. Our results indicate that most damage to artificial seedlings arises from larger vertebrates but that most spatial variation in this damage reflects the impacts of falling plant debris. These damage processes appear sufficient to constrain the regeneration of tree species in areas where forest cover is patchy and fragmented.

Infrequent Occurrence of Peach Skin Streaking and the Role of Rainwater Attributes on Symptom Development.

Streaks lacking pigmentation have impacted red blush cultivars of peaches in many East Coast production areas, but the underlying cause is still unclear. Some evidence suggests that streaking may be caused by reactive agents in rainwater. Peach skin streaking was monitored over two consecutive years at a commercial farm with a history of streaking problems located near Ridge Spring, SC. Six cultivars (two early season, two midseason, and two late season) were evaluated, each in two locations (LocA and LocB). Among those 12 experimental block cultivars, streaking occurred only in 2017 in cv. Scarletprince of LocA with an incidence of 6%. That same year two nearby nonexperimental blocks with 'Scarletprince' revealed 11 and 25% streaking. Streaking was also monitored at the Musser Fruit Research Center (MFRC) in Seneca, SC. At that location, a high incidence of streaking was observed, with 50 and 64% in 'Julyprince' (2017) and 'Carored' (2018), respectively. Rainwater pH taken from each of the 12 experimental blocks ranged from 3.03 to 7.4, ozone (O3) levels ranged from <0.02 to 0.37 mg/liter, and chlorine (Cl2) and chlorine dioxide (ClO2) levels were either just above or under the detection limit of 0.01 mg/liter and 0.02 mg/liter, respectively. Although the electrical conductivity (EC) was below 100 µS/cm on average, we did measure EC values as high as 1,500 µS/cm. For all samples, the oxidation-reduction potential (ORP) ranged from 90 to 302 mV, indicating oxidizing conditions. Fruit harvested 1 or 2 weeks prior to commercial maturity and treated with solutions of high (10) or low (3) pH, ozone >0.37 mg/liter, and EC values of up to 3,000 µS/cm did not produce symptoms. However, streaking was reproduced with collected rainwater, but the remaining sample volume did not allow further analyses. Using 0.05% ClO2 to induce streaking, we show that fruit of different cultivars varied in susceptibility when treated 1 week prior to commercial maturity, with 'Juneflame' being the most susceptible and 'August Lady' being the least susceptible. Our study shows that multiple factors determine the occurrence of streaking in peach orchards, including cultivar susceptibility, ripening stage, and the presence of rainwater with sufficient amounts of a yet unknown reactive agent or agent combination.

Connectivity-preserving flocking for networked Lagrange systems with time-varying actuator faults

This paper studies fault-tolerant coordination with connectivity preservation and collision avoidance for unknown Lagrange agent systems subject to actuator faults. In contrast to the existing works, the actuator faults may change the states of the agents to violate the network connectivity and the existence of the loss of effectiveness faults makes control coefficients unknown and time-varying. Furthermore, the leader’s dynamics are allowed to be nonlinear instead of being linear and generated by a marginally stable system. The aforementioned setting improves the practical relevance of the problem to be addressed in the paper and meanwhile, it poses technical challenges to flocking controller design and asymptotic stability analysis. Consequently, for a class of nonlinear leader systems, a novel distributed adaptive scheme is proposed by introducing a distributed estimator and a Nussbaum gain technique to provide a fully distributed solution with guaranteed fault-tolerant coordination, connectivity preservation, and collision avoidance simultaneously. Numerical examples are given to illustrate the effectiveness of the design.

Unknown Agents in Friends Oriented Hedonic Games: Stability and Complexity

We study hedonic games under friends appreciation, where each agent considers other agents friends, enemies, or unknown agents. Although existing work assumed that unknown agents have no impact on an agent’s preference, it may be that her preference depends on the number of unknown agents in her coalition. We extend the existing preference, friends appreciation, by proposing two alternative attitudes toward unknown agents, extraversion and introversion, depending on whether unknown agents have a slightly positive or negative impact on preference. When each agent prefers coalitions with more unknown agents, we show that both core stable outcomes and individually stable outcomes may not exist. We also prove that deciding the existence of the core and the existence of an individual stable coalition structure are respectively NPNP-complete and NP-complete.

063 Headache with periorbital ecchymosis; a rare clinical entity

ObjectivesPrimary headache disorders are common with migraine and tension headache accounting for the vast majority of cases. A smaller proportion suffer from trigeminal autonomic cephalgia (TAC). We present a 23-year-old Caucasian female who described characteristic, episodic headaches starting with a dull retro-orbital/bi-frontal pressure evolving, over the course of 1 minute, to experience florid periorbital ecchymosis. While this phenomenon has been described in the literature, the characteristics of our case are unique and noteworthy of reporting.MethodsWe reviewed the literature surrounding this rare entity by using PubMed/OVID databases and the search terms ‘Headache AND ecchymosis’.ResultsCase reports exist in older patients1–3, where the headache is side locked and associated with other autonomic characteristics such as periorbital oedema, conjunctival injection and tearing. Our case is a young female with only ecchymosis in a unilateral and/or bilateral manner and no other autonomic or indeed migraine features. The patient underwent vascular/cranial imaging and blood tests to exclude haematological, autoimmune, vasculitic causes for this presentation which were unrewarding.ConclusionVariations on this clinical entity are described;1–4 we hope this report may bring attention to this fascinating phenomenon. The pathophysiological process is likely to be similar to those implicated in TACs, namely activation of the trigemino-neurovascular system and facial autonomic pathways. The release of neuromediators such as CGRP, VIP and Substance P cause blood vessel fragility resulting in diapedesis. Optimal treatment regimens are unknown but various agents have been trialled. Our patient declined treatment and continues to be followed.ReferencesDeBroff B, et al. Migraine Associated with Periorbital Ecchymosis. Headache 1990;30:260–263.Dafer R, et al. Atypical Chronic Headache and Recurrent Facial Ecchymosis: A Case Report. Neuro-Ophthalmology 2011;35:76–77.Nozzolillo D, et al. Migraine associated with facial ecchymoses ipsilateral to the symptomatic side. J Headache Pain ( 2004) 5:256–259.Sethi PK, et al. Teaching neuroimages: Red forehead dot syndrome and migraine revisited. Neurology 2015;85;e28.

Seasonality of i.v. immunoglobulin responsiveness in Kawasaki disease.

Evidence suggests that seasonal variation in the onset of Kawasaki disease (KD) exists worldwide. Whether a seasonal component to successful i.v. immunoglobulin (IVIG) therapy exists in KD-positive children, however, is unknown. We addressed this question by focusing on patients with primary onset KD who were non-responsive to IVIG treatment, in the large nationwide Japanese KD survey datasets from 2009 to 2016. In these datasets, the IVIG therapy non-responders were defined as patients whose fever persisted ≥24 h or recurred ≤24 h after the end of the initial IVIG treatment (dosage, 2,000mg/kg). Those who successfully responded to this treatment were defined as IVIG responders. The consecutive monthly trend of the proportion of IVIG non-responders was analyzed throughout the study period to investigate seasonal periodicity on Fourier analysis, and the monthly distributions of non-responders and responders were compared. From a total of 113691 KD-positive patients, 15.7% were IVIG non-responders, and 61% were male. The proportion of non-responders increased across each calendar year with fluctuation, and Fourier analysis indicated seasonal periodicity. The seasonality effect differed between responders and non-responders, with the proportion of responders tending to increase in autumn through winter, while the non-responders showed a decreasing trend in autumn. The seasonality effect tended to differ by sex. The results indicate that the currently unknown etiological agents of KD might differ between IVIG responders and non-responders. In addition, immune reactivity against such agents possibly differs by sex in the IVIG non-responders.

Comparison of diffusion, cytotoxicity and tissue inflammatory reactions of four commercial bleaching products against human dental pulp stem cells

Multiple side effects related to bleaching were found to occur in the dental pulp tissue, including decreased cell metabolism and viability. In this work we evaluated the in vitro diffusion capacity, cytotoxicity and biocompatibility of four commercial bleaching products on stem cells from human dental pulp (hDPSCs). Two commercial bleaching gels hydrogen peroxide-based (HP), Norblanc Office 37.5% (Nor-HP) and Opalescence Boost 40% (Opal-HP) were applied for 30 min to enamel/dentine discs. Another two gels from the same manufacturers, 16% carbamide peroxide-based (CP), Norblanc Home (Nor-CP) and Opalescence CP 16% (Opal-CP), were applied for 90 min. The diffusion of HP was analysed by fluorometry. Cytotoxicity was determined using the MTT assays, the determination of apoptosis, immunofluorescence assays and intracellular reactive oxygen species (ROS) level. Tissue inflammatory reactions were evaluated histopathologically in rats. Statistical differences were performed by one-way ANOVA and Bonferroni post-test (α < 0.05). Normon products showed lower cytotoxicity and diffusion capacity than the Ultradent products. A high intracellular ROS level was measured in hDPSCs after exposure to Opal-HP. Finally, a severe necrosis of both coronal and radicular pulp was observed with Opal-HP. Similar concentrations of hydrogen peroxide and carbamide peroxide in a variety of bleaching products exhibited different responses in cells and dental pulp tissue, suggesting that bleaching products contain unknown agents that could influence their toxicity.

Multi‐layer distributed protocols for robust cooperative tracking in interconnected nonlinear multiagent systems

SummaryWe develop a mixed graph and optimal control theoretic formulation to design a robust cooperative control protocol for a large‐scale multiagent system with partially known interconnected first‐, second‐, or mixed first‐ and second‐order dynamics. In each case, we transform the control protocol design task to a robust communication graph design problem, which, from a cyber‐physical perspective, is interpreted as the control layer design problem for an interconnected system with unknown agent layer dynamics. According to this viewpoint, each state variable has its own control layer communication topology separate from the other state variable's communication topology and the unknown agent layer interconnection topologies. We prove that all cooperative, decentralized, and centralized tracking protocols can be treated as a single design problem and, by deriving closed‐form solutions for the robust control layer topologies, we further provide a simpler design procedure, which is only based on the matrix manipulations. Aside from the linear implementation of the protocol and the connection of the proposed formulation to the well known rules‐of‐thumb in optimal control theory, this creates a higher potential to transfer ideas to industry. Modeling uncertainties tolerable by a given control layer topology is analyzed, and a preliminary performance‐oriented analysis and design approach for large‐scale interconnected systems is discussed. We show that exactly the same steps can be followed to design appropriate control layers for both tracking and stabilization.

The Causes of Canine Myocarditis and Myocardial Fibrosis Are Elusive by Targeted Molecular Testing: Retrospective Analysis and Literature Review.

Myocarditis can cause death or permanent heart damage. As epidemiologic and etiopathologic data for canine myocarditis are lacking, we performed a retrospective study using nucleic acid extracted from archived (2007 to 2015) tissues from myocarditis cases and control dogs without myocardial lesions. Heart tissue from pediatric/juvenile and adult dogs was tested with a comprehensive panel of conventional and real-time polymerase chain reaction (PCR) assays targeting recognized agents of canine myocarditis based on a literature review and informed by the comparative epidemiology of human myocarditis. The PCR screen, which included canine parvovirus 2 (CPV-2), canine distemper virus, canine herpesvirus, Borrelia spp, West Nile virus, adenovirus, parainfluenza virus, pneumovirus, respiratory coronavirus, influenza virus, Bartonella spp, Rickettsia spp, Mycoplasma spp, and Neospora caninum, did not detect agents in 35 of 66 cases (53%; 95% confidence interval [CI], 41%-65%) and was frequently negative in adults (21/26); by comparison, agents were not detected in 27 of 57 controls (47%; 95% CI, 35%-60%). Canine distemper virus, herpesvirus, adenovirus, coronavirus, parainfluenza virus, Mycoplasma haemocanis, and N. caninum were occasionally detected in both cases and controls; thus, PCR detection was not considered to indicate causation. We previously reported that CPV-2 continues to be associated with myocarditis in young dogs despite widespread vaccination; in adults, CPV-2 was detected in 2 of 26 cases and 4 of 22 controls. As several agents were similarly detected in cases and controls, it is unclear if these are cardiopathogenic, incidental, or latent. West Nile virus was detected at the analytic limit in 1 adult case. We did not detect Borrelia spp, Bartonella spp, Rickettsia spp, or influenza A virus in the myocarditis cases. These data demonstrate the limitations of current targeted diagnostic tests and the need for additional research to identify unknown agents and develop testing strategies for canine myocarditis.

Parachlamydia acanthamoebae Detected during a Pneumonia Outbreak in Southeastern Finland, in 2017⁻2018.

Community-acquired pneumonia (CAP) is a common disease responsible for significant morbidity and mortality. However, the definite etiology of CAP often remains unresolved, suggesting that unknown agents of pneumonia remain to be identified. The recently discovered members of the order Chlamydiales, Chlamydia-related bacteria (CRB), are considered as possible emerging agents of CAP. Parachlamydia acanthamoebae is the most studied candidate. It survives and replicates inside free-living amoeba, which it might potentially use as a vehicle to infect animals and humans. A Mycoplasma pneumoniae outbreak was observed in Kymenlaakso region in Southeastern Finland during August 2017–January 2018. We determined the occurrence of Chlamydiales bacteria and their natural host, free-living amoeba in respiratory specimens collected during this outbreak with molecular methods. Altogether, 22/278 (7.9%) of the samples contained Chlamydiales DNA. By sequence analysis, majority of the CRBs detected were members of the Parachlamydiaceae family. Amoebal DNA was not detected within the sample material. Our study further proposes that Parachlamydiaceae could be a potential agent causing atypical CAP in children and adolescents.

Identification and characterization of water chestnut Soymovirus-1 (WCSV-1), a novel Soymovirus in water chestnuts (Eleocharis dulcis)

BackgroundA disease of unknown etiology in water chestnut plants (Eleocharis dulcis) was reported in China between 2012 and 2014. High throughput sequencing of small RNA (sRNA) combined with bioinformatics, and molecular identification based on PCR detection with virus-specific primers and DNA sequencing is a desirable approach to identify an unknown infectious agent. In this study, we employed this approach to identify viral sequences in water chestnut plants and to explore the molecular interaction of the identified viral pathogen and its natural plant host.ResultsBased on high throughput sequencing of virus-derived small RNAs (vsRNA), we identified the sequence a new-to-science double-strand DNA virus isolated from water chestnut cv. ‘Tuanfeng’ samples, a widely grown cultivar in Hubei province, China, and analyzed its genomic organization. The complete genomic sequence is 7535 base-pairs in length, and shares 42–52% nucleotide sequence identity with viruses in the Caulimoviridae family. The virus contains nine predicated open reading frames (ORFs) encoding nine hypothetical proteins, with conserved domains characteristic of caulimoviruses. Phylogenetic analyses at the nucleotide and amino acid levels indicated that the virus belongs to the genus Soymovirus. The virus is tentatively named Water chestnut soymovirus-1 (WCSV-1). Phylogenetic analysis of the putative viral polymerase protein suggested that WCSV-1 is distinct to other well established species in the Soymovirus genus. This conclusion was supported by phylogenetic analyses of the amino acid sequences encoded by ORFs I, IV, VI, or VII. The sRNA bioinformatics showed that the majority of the vsRNAs are 22-nt in length with a preference for U at the 5′-terminal nucleotide. The vsRNAs are unevenly distributed over both strands of the entire WCSV-1 circular genome, and are clustered into small defined regions. In addition, we detected WCSV-1 in asymptomatic and symptomatic water chestnut samples collected from different regions of China by using PCR. RNA-seq assays further confirmed the presence of WCSV-1-derived viral RNA in infected plants.ConclusionsThis is the first discovery of a dsDNA virus in the genus Soymovirus infecting water chestnuts. Data presented also add new information towards a better understanding of the co-evolutionary mechanisms between the virus and its natural plant host.

Generalisation of Anthropomorphic Stereotype

Anthropomorphic projection can bring familiarity, confidence and simplicity to our interactions with unknown agents showing a human-like resemblance or behaviour. This study examined whether this projection is generalised beyond the individual agent to encompass others of similar type, even if they might be lacking the requisite human-like features. In an experiment participants had to accept or reject recommendations from two robots that had more or less human-like forms and behaviours. It was found that participants were more likely to trust the judgements of a less-human like robot if they had previously interacted with an anthropomorphic variant. Importantly, this effect was found to be symmetric, with trust in anthropomorphic robots reduced if participants had previously interacted with a less human-like variant. These results showed that we generalise our initial attributions across agent categories, a finding with potential application in helping trust and acceptance of complex technological agents.

Adaptive Leader Following in Networks of Discrete-Time Dynamical Systems: Algorithms and Global Convergence Analysis

Most of the results on adaptive leader following in multiagent systems pertain to continuous-time agent dynamics. It is implicitly presumed that the information flow among agents is continuous and infinitely fast. Since in the discrete-time setting, there no need for this assumption, it is of interest to develop adaptive methods for leader-follower synchronization in networks of discrete-time dynamical systems. There main challenges are as follows: 1) in general, the sample values of the leader's trajectory arrive at each agent with different delays; 2) since the received leader's trajectory samples do not carry the imprint of a leader, and are immersed in the state values of respective neighbors, agents do not have explicit knowledge of the leader's samples; and 3) the uncertainty in the agent dynamics calls for an appropriate adaptive rule for generating interagent coupling gains. This paper considers unknown linear agent dynamics of general order and arbitrary leader's trajectory, on a directed graph topology. Two novel distributed algorithms are proposed for both cases of known and unknown control directions. It is proven that all agents synchronize to the leader's trajectory, the tracking error is an $l_2$ sequence, the input signals remain bounded, and the parameter estimates are convergent sequences.

Criticism From Artificial Agents: Prior Interaction Reduces Negative Effects

The present study explored interaction between humans and artificial agents from a relational perspective. According to theories on interpersonal communication and relationships, criticism by known agents, as compared to unknown agents, could be less negative (the buffering effect of relationship) or more negative (the expectancy violation effect) to human-agent relationships. Using online survey tools, participants who had interacted with an agent were led to one of the same or different agent conditions, in which the same or a new agent gave negative comments (criticism) to participants. Participants evaluated the agent and the interaction with it before and after receiving the criticism. Results showed that a brief acquaintanceship with agents lessened the negative effects of criticism. Participants’ evaluations decreased to a lesser degree when the negative comments were given by the acquaintance agent rather than the stranger agent. Thus, the buffering effect of relationship was supported in human-agent relationships.

Latinas for Trump Analysis of Processes of Identification and the Use of Narratives to Construct Subject-Positions

Abstract The public and commercial spheres constantly address the largest ethnic minority in the United States, people with ancestry or from a Latin American country, as a homogenous group under the ethnopolitical terms “Latinos,” “Hispanics,” and even “Mexicans.” This panethnic view, and the negative stereotypes associated with it, was especially visible during the 2016 presidential election. While the majority of Latinos found Donald Trump’s remarks on “Mexicans” offensive to the Latin community as a whole, a large number of people still supported his opinions, even those belonging to the “Latino” community. Even more so, women of Latino heritage still supported a nominee that went against their own advance in society given his constant misogynistic comments. In this essay, I analyze the groundings for this apparent contradiction in the preference for said candidate. I argue that these women’s political preference is a tool with which they build their identity in the U.S. Besides, I explore the ways in which individuals linguistically construct their own identity in three ways (i) by actively doing the identification instead of merely receiving it by an unknown agent; (ii) by choosing the self-representation of their preference, and (iii) by finding commonalities and bonding with other individuals they deem part of their group. Through this approach, I analyze semiotic processes, such as intertextuality, use of pronouns, and discourse alignment, that are used to construct identifications of the self that go beyond imposed categories, such as gender and ethnicity.

Effect-based assessment of recipient waters impacted by on-site, small scale, and large scale waste water treatment facilities \u2013 combining passive sampling with in vitro bioassays and chemical analysis

Waste water treatment facilities are a major sources of organic micropollutants (MPs) in surface water. In this study, surface water samples were collected from seven sites along a river system in Uppsala, Sweden, during four seasons and evaluated based on the occurrence of MPs in the samples and bioactivity using in vitro bioassays. The sampling sites were differentially impacted by on-site sewage treatment facilities (OSSFs), small scale, and large scale waste water treatment plants (WWTPs). The bioassays used included activation of aryl hydrocarbon receptor (AhR), estrogen receptor (ER), nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB), nuclear factor erythroid 2-related factor 2 (Nrf2), and androgen receptor (AR). Occurrence of 80 MPs, were analyzed using liquid chromatography coupled to tandem mass spectrometry. Most water samples induced AhR activity, and all sampling sites showed a similar profile regarding this activity. With the exception of one water sample, we did not detect any NFkB, Nrf2 or AR activity of the water samples. The exception was a sample impacted by OSSFs, which showed an activity in multiple bioassays, but the activity could not be explained by the occurrence of target MPs. The occurrence of MPs showed a spatial trend, with the highest number and amount of MPs detected in the samples collected downstream of the WWTPs, where up to 47 MPs were detected in one single sample. A seasonal variation was observed with highest levels of MPs and highest AhR activities in samples collected in June and September 2015. However, neither the seasonal activity nor the on-site activity could be explained by the measured MPs, suggesting unknown contributory agents in the water.