The increasing contribution of refrigeration systems to global warming has led to widespread demand for low global warming potential (GWP) refrigerants as alternatives. This paper proposed a comprehensive environmental assessment method for evaluating the carbon footprint of units charged with low-GWP refrigeration. Experimental and numerical studies were used to investigate the carbon footprint impact of using alternative refrigerants at different ambient temperatures in China. Initially, the model was validated through experimental case studies on the use stage of cold storage units. Subsequently, a comparison was made between the cooling capacity (CC) and energy efficiency ratio (EER) of cold storage units charged with different refrigerants. Additionally, the impact of alternative refrigerants in the use stage was analyzed by numerical calculations, and the adaptability of the cold storage unit to the ambient temperature and the national energy structure was also studied. The results indicate that among the four stages of the cold storage unit, the use stage is the hotspot of carbon footprint due to the large amount of energy consumption and the existence of refrigerant leakage and other behaviors. Utilizing low-GWP alternative refrigerants could significantly reduce the carbon footprint of the cold storage units' use stage by 10.56%–16.39%, thereby lowering the whole stage carbon footprint. Furthermore, the ambient temperature and national energy structure could also effectively reduce the carbon footprint of cold storage units' whole stage by 1%–27.17%. Furthermore, after adopting low GWP refrigerants, the life-cycle cost (LCC) of the units have also decreased by 6.1%–12%. Taking into account both environmental impact and economic considerations, it is recommended to directly replace R404A with R448A in the units in regions where the average ambient temperature is above 15 °C.
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