Uninfected KB Research Articles

Histoenzymatic activities of three KB cell lines latently infected by Myxovirus parainfluenzae, type 3, strain EA-102. II. Hydrolytic and oxidative enzyme activity.

Seventeen tests were performed in situ to investigate a part of the enzymatic system of three KB cell lines latently infected by Myxovirus parainfluenzae, type 3, strain EA-102. The activity of 5 hydrolytic and 12 oxidative enzymes is reported. No metabolic change was detected by histoenzymatic techniques in a comparative study of the three KB cell lines latently infected by the virus or in the uninfected KB cells. Furthermore no enzymatic difference was observed between a cell line of neoplastic origin (KB) and a non-neoplastic chorionic cell line (Laval 113). Six of the 17 enzymatic tests were questionable or negative when conducted on primary monkey kidney cells (MK).

RNA of low molecular weight in KB cells infected with adenovirus type 2

Chromatographic analysis on methylated albumin—kieselguhr columns of newly formed, radioactive RNA from KB cells infected with adenovirus type 2 revealed an unusual radioactive component which was eluted following 4 s RNA. This RNA was present predominantly in the 100,000 g ribosomal supernatant cell fraction, and was distinguished from 4 s, 16 s and 28 s RNA's on the basis of its behavior on methylated albumin columns, base composition, sedimentation velocity and behavior on Sephadex G100 columns. This RNA component has certain characteristics in common with a “5 s” RNA associated with ribosomes from uninfected KB cells and a variety of other organisms. The effect of deoxy-fluorouridine and actinomycin D on the metabolism of this unusual RNA suggests that DNA synthesis and transcription of DNA into RNA are required for its formation.

RNA production in adenovirus-infected KB cells

Ribonucleic acid (RNA) from adenovirus type 2-infected KB cells was examined with DNA-RNA homology techniques ( Nygaard and Hall, 1963), sucrose gradient analysis and methylated albumin column chromatography. The presence of viral complementary RNA was detected as early as 9 hours after infection, and relatively larger amounts were present 28 hours after infection. Although sucrose gradient analysis revealed no differences in radioactive labeling of RNA from infected and uninfected KB cells, an unusual nucleic acid component appeared in RNA from infected KB cells when analysis was performed with methylated albumin column chromatography. This component was absent from uninfected cell cultures.