Unimodal Distribution Research Articles

170 Disc Space Height in Relation to Neural Foraminal Dimensions and Patient Characteristics: A Morphometric Analysis From L1-S1 Using Computed Tomography

INTRODUCTION: The normative relationship between lumbar intervertebral disc space height (DSH) and neuroforaminal dimensions (NFD) has yet to be defined. METHODS: Anterior, middle, and posterior DSH were measured on 350 female and 350 male patients between 18 and 35 years of age without spinal pathology. NFD were defined as sagittal anterior-to-posterior (AP) width, axial AP width, foraminal height, and area. Statistical analyses were performed to assess associations among DSH, NFD, and patient height, weight, body mass index, sex, and ethnicity. RESULTS: DSH measurements demonstrated increasing, linear trends moving caudally from L1-L2 to L5-S1, while NFD demonstrated a unimodal distribution pattern with largest NFD at L3-L4 and smallest NFD at L1-2 and L5-S1. Accounting for confounding due to height, weight, and ethnicity, male patients demonstrated larger DSH compared to female patients across all levels L1-S1 (p < .001). Asian patients demonstrated taller DSH compared to Caucasian, Hispanic, and African-American patients across all levels L1-S1 (p = .014). Zero moderate or strong correlations were observed between DSH and NFD measurements or patient characteristics for all levels L1-S1, though weight and foraminal height were weakly (r = .3) associated with increased DSH (p < .001). CONCLUSIONS: This study describes 38,500 CT-based L1-S1 disc space height and neuroforaminal measurements in young patients without spinal pathology. Among these patients, disc space height follows an increasing trend moving caudally from L1-S1, while foraminal dimensions demonstrate a unimodal distribution clustered at L3-L4. Neuroforaminal dimensions, including foraminal height, are not moderately or strongly associated with disc space height. Disc space height is influenced by sex and ethnicity but is not moderately or strongly influenced by patient height, weight, and BMI.

Spatiotemporal variability of streamflow in the Pearl River Basin: Controls of land surface processes and atmospheric impacts

AbstractTo advance understanding of the spatiotemporal variability of the streamflow in the Pearl River Basin (PRB), we used the Soil and Water Assessment Tool model to simulate the water fluxes from 2010 to 2020 and to identify the underlying controlling mechanisms. The streamflow in the PRB is highly variable and controlled by complex land surface processes and atmospheric impacts over the heterogeneous terrain. Two key factors primarily govern the streamflow: (1) the location of the active precipitation zone, which is determined by the interaction between the monsoon path and uplifting effect of the terrain, and (2) the redistributions resulting from land use and soil characteristics. We observe distinct patterns in the different water fluxes across the different regions. Specifically, surface flow exhibits the highest activity within the precipitation zone. Lateral flow and actual evapotranspiration (AET) have the greatest intensity in the forests and in agricultural regions, respectively, and the aquifer flow is more active in areas with coarse soil textures. The land surface processes of the AET and aquifer retention significantly govern the temporal variability of the streamflow, contributing to the precipitation and streamflow being out of phase in the PRB. Based on the underlying mechanisms driving streamflow variability, we classify the PRB into three substreams: a drought‐prone upstream, a hydrologically active midstream, and a typhoon‐affected downstream, and each substream exhibits distinct spatiotemporal characteristics of streamflow. We find that the time series and probability distributions of the streamflow at different tributaries within each substream are similar. Each probability distribution is multimodal and can be decomposed into three unimodal distributions representing dry, transitional, and wet conditions. Specifically, the PRB features a large and steep dry mode, a flat transitional mode, and a short wet mode.

Lake Surface Temperature Retrieval Study Based on Landsat 8 Satellite Imagery—A Case Study of Poyang Lake

Poyang Lake is the largest freshwater lake in China and forms an essential component of the hydrological, nutrient, and carbon cycles, providing various ecosystem services to the local environment. Since changes in Poyang Lake’s water temperature can significantly affect the surrounding environment and social development, continuous monitoring of lake temperature changes is required. Traditional water monitoring methods are resource intensive and cannot simultaneously conduct extensive water monitoring. Remote sensing of temperature inversion has the advantages of all-weather, efficient, and large-scale real-time monitoring. Six Landsat 8 images from August to October in 2020 and 2021 were utilized to extract lake surface temperature (LST), and the variations in LST over the two years were analyzed to determine the impact of global climate anomalies on inland lakes. The results indicate that the LST in August and October 2021 was significantly higher than that in the same periods of the previous year, and the temperature difference in October reached 8 °C. In contrast to the overall normal distribution pattern of the water temperature in 2020, 2021 exhibited a relatively concentrated, unimodal distribution pattern. A trend analysis of the driving factors suggests that the LST of Poyang Lake is influenced by the global climate, and the artificial heat sources around the lake clearly alter the distribution characteristics of the LST simultaneously.

Promotive effects of marine-derived dimethyl sulfoxide on the photodegradation of phenanthrene in the atmosphere

Dimethyl sulfoxide (DMSO), a versatile medium, is a particular component in the marine atmosphere that possibly causes polycyclic aromatic hydrocarbons (PAHs) to degrade differently than they do in the continental atmosphere. In this study, phenanthrene (Phe) was used as a model PAH in batch photochemical experiments to investigate the chemical actions of DMSO and the underlying mechanisms. The photodegradation of Phe in aqueous solutions with DMSO volume fractions from 0 % to 100 % was initiated by ultraviolet (UV) radiation and promoted by singlet oxygen, which was consistent with pseudo-first-order kinetics. Phe photodegraded faster in a mixture of DMSO and water than in water or DMSO alone, and the rate constant showed a unimodal distribution over the DMSO fraction range, peaking at 33 % DMSO (0.0333 ± 0.0009 min−1) and 40 % DMSO (0.0199 ± 0.0005 min−1) under 254 nm and 302 nm UV radiation, respectively. This interesting phenomenon was attributed to the competition of DMSO for UV radiation and singlet oxygen and changes in dissolved oxygen and free water contents caused by the interaction between DMSO and water molecules. In addition, 9,10-phenanthrenequinone (9,10-PhQ) with high cytotoxicity was the main photodegradation product of Phe under various conditions. The photodegradation rate of Phe in the mixtures of DMSO and water was comparable to its reaction rate with OH radicals, suggesting that 9,10-PhQ can be rapidly generated in the marine atmosphere, driven by a mechanism different from that in the continental or urban atmosphere. Under the presented experimental conditions, UV intensity and DMSO fraction were the primary factors that affected the photodegradation rate of Phe and 9,10-PhQ and altered their integrated toxicity. The findings of this study support the conclusion that the marine atmosphere is an essential field in the atmospheric transport of PAHs, in which DMSO is an important component that affects their photodegradation.

The fluidization behavior of particles in supercritical water fluidized bed: A region-dependent analysis

The distribution and fluctuation of particle concentration in a supercritical water fluidized bed (SCWFB) are studied using a specially-designed capacitance probe measurement system. The measurement error of capacitance probe system is 2.48%. Results show that the particle concentration at the axial line fluctuates largely with time and the fluctuation is weakened when increasing the distance from axial line or decreasing particle diameter. At Uf/Umb=1.5, the largest difference between particle concentrations is 0.026, 0.04, 0.082 when the particles with a diameter of 0.124 mm, 0.198 mm, 0.235 mm are used, indicating a larger non-uniformity of larger particles. The probability density of local particle concentration mainly follows a unimodal distribution with the peak at right side. The main frequency of SCWFB ranges from 1.39 Hz to 2.73 Hz in this experiment and the main frequency decreases when larger particles are used.

Mannose-Decorated Solid-Lipid Nanoparticles for Alveolar Macrophage Targeted Delivery of Rifampicin.

Alveolar macrophages play a vital role in a variety of lung diseases, including tuberculosis. Thus, alveolar macrophage targeted anti-tubercular drug delivery through nanocarriers could improve its therapeutic response against tuberculosis. The current study aimed at exploring the efficacy of glyceryl monostearate (GMS)-based solid-lipid nanoparticles (SLNs) and their mannose functionalized forms on the alveolar macrophage targeting ability of an anti-tubercular model drug, rifampicin (Rif). Rif-loaded SLNs were accomplished by the solvent diffusion method. These carriers with unimodal particle size distribution (~170 nm) were further surface-modified with mannose via Schiff-base reaction, leading to slight enhancement of particle diameter and a decline of drug loading capacity. The encapsulated Rif, which was molecularly dispersed within the matrices as indicated by their XRD patterns, was eluted in a sustained manner with an initial burst release effect. The uptake efficiency of mannose-modified SLNs was remarkably higher than that of corresponding native forms on murine macrophage Raw 264.7 cells and human lung adenocarcinoma A549 cells. Eventually, the mannose-modified SLNs showed a greater cytotoxicity on Raw 264.7 and A549 cells relative to their unmodified forms. Overall, our study demonstrated that mannose modification of SLNs had an influence on their uptake by alveolar macrophages, which could provide guidance for the future development of alveolar macrophage targeted nanoformulations.

Oil-in-water nanoemulsions loaded with lycopene extracts encapsulated by spray drying: Formulation, characterization and optimization

Lycopene is very susceptible to degradation once released from the protective chromoplast environment. In this study the oil-in-water (O/W) nanoemulsions coupled with spray drying technology was applied for the encapsulation and stabilization of lycopene extracted from tomato waste. Tomato extract was obtained by ultrasound-assisted extraction. Nanoemulsions were prepared by high-speed rotor stator using isopropyl myristate as oil phase and Pluronic F-127 as emulsifier for the aqueous external phase. The effect of emulsification process parameters was investigated. Spray drying of produced emulsions was attempted to obtain a stabilized dry powder after the addition of a coating agent. The effect of different coating agents (maltodextrin, inulin, gum arabic, pectin, whey and polyvinylpyrrolidone), drying temperature (120–170 °C) and feed flow rate (3–9 ml·min–1) on obtained particles was evaluated.Results revealed that the emulsion formulation of 20/80 (O/W) with 1.5% (mass fraction) of Pluronic F-127 as stabilizer in the aqueous phase resulted in stable nanoemulsion with droplets size in the range (259–276 nm) with unimodal and sharp size distribution. Extract in the nanoemulsion was well protected at room temperature with a degradation rate of lycopene of about 50% during a month of storage time. The most stable emulsions were then processed by spray drying to obtain a dry powder. The spray drying was in particular successful when using maltodextrin as coating agent, obtaining dried spherical particles with mean diameters of 4.87±0.17 μm with a smooth surface. The possibility of dissolving the spray dried powder in order to repristinate. The original emulsion was also successfully verified.

Early Miocene sand wedge deposits in Southwestern Tarim Basin and Implications for the Uplift of the Northern Tibetan Plateau

The uplift of the Tibet Plateau and its climatic effects have long been a hot topic in academic research. However, there is still significant controversy regarding the uplift history and related climate changes along the northern margin of the plateau. In this paper, we present sand wedge deposits from the base of the Wuqia Group (∼20 Ma) on the southwestern Tarim Basin, representing the oldest reported sand wedges in the Tibetan Plateau. A comprehensive study was conducted on lithostratigraphy, sedimentary environment, and macroscopic–microscopic features of the sand wedge deposits. The results indicate that the lithostratigraphic succession preserving the sand wedge deposits consists mainly of lenticular conglomerates and cross-bedded gravelly sandstone interbedded with medium–thin-bedded mudstone, consistent with the braided fluvial environment. The sand wedge deposits commonly exhibit a V-shape geometry and are filled with gravelly sandstone, with the gravels arranged parallel to the walls of the wedge. The clasts of the wedge-filled sandstone are well-rounded and show a unimodal grain size distribution. The quartz surfaces commonly display dish-shaped and crescent-shaped impact features, indicating an eolian origin and an arid climate. Based on the relationship between the formation temperature of modern sand wedges, the atmospheric temperature gradient, and altitude, the reconstructed paleo-elevation of the sand wedge deposits at the base of the Wuqia Group in the southwestern Tarim Basin is approximately ∼ 1000 m. These results indicate that the northern margin of the Tibet Plateau had already reached an elevation of ∼ 1000 m during the Early Miocene, forming a seasonal arid and cold climate.

The Flexible Gumbel Distribution: A New Model for Inference about the Mode

A new unimodal distribution family indexed via the mode and three other parameters is derived from a mixture of a Gumbel distribution for the maximum and a Gumbel distribution for the minimum. Properties of the proposed distribution are explored, including model identifiability and flexibility in capturing heavy-tailed data that exhibit different directions of skewness over a wide range. Both frequentist and Bayesian methods are developed to infer parameters in the new distribution. Simulation studies are conducted to demonstrate satisfactory performance of both methods. By fitting the proposed model to simulated data and data from an application in hydrology, it is shown that the proposed flexible distribution is especially suitable for data containing extreme values in either direction, with the mode being a location parameter of interest. Using the proposed unimodal distribution, one can easily formulate a regression model concerning the mode of a response given covariates. We apply this model to data from an application in criminology to reveal interesting data features that are obscured by outliers.

Does the temperature–size rule apply to idiobiont parasitoids?

AbstractIn most ectotherms, several life‐history traits, including body size, respond to environmental conditions through the temperature–size rule (TSR). The mechanisms underlying the TSR are still being debated, but studying idiobiont insect parasitoids, which develop with a fixed amount of resources, may shed light on this relationship. In this study, we conducted experiments to determine how the developmental temperature affects various characteristics of male and female Trichogramma euproctidis (Girault) (Hymenoptera: Trichogrammatidae), an idiobiont egg parasitoid of Lepidoptera. The study tested various hypotheses, including the cellular or oxygen diffusion hypotheses and the resource acquisition hypothesis, to understand whether T. euproctidis follows the TSR. The developmental time of both male and female T. euproctidis decreased with increasing temperature. Both males and females displayed a unimodal distribution for size, dry mass, and lipid content, with individuals at lower and higher temperatures being smaller, weighing less, and containing fewer lipids. Female lifetime fecundity increased from 13 to 24 °C and then decreased at 31 °C. Additionally, the number and size of gametes in male and female T. euproctidis displayed a unimodal distribution with increasing temperature. Trichogramma euproctidis deviates from the TSR as it follows a non‐linear reaction norm with an optimal developmental temperature. This result supports the hypothesis that for species following TSR and having unlimited access to food resources, the resource acquisition hypothesis is a significant mechanism explaining the TSR. With climate change affecting temperature, understanding the TSR is crucial, and research on insect parasitoids may help reveal how the interplay between environmental temperature and resource allocation affects the TSR in natural populations.

Bayesian semi-parametric estimation of compound inhomogeneous Poisson processes for ultra-high frequency financial transaction data

Marked point processes provide a flexible framework for studying ultra-high frequency financial data that records the time and price for each transaction. This paper estimates compound, inhomogeneous Poisson processes (CIPP) where trades arrive according to an inhomogeneous Poisson process, and returns are drawn from a distribution after arrival. The intensity functions have Gaussian process priors to model time-of-day (diurnal) effects, which have bursts of activity at the open and close and a midday lull, and lagged returns from the previous trade, which are idiosyncratic and depend on the stock. The nonparametric density estimator for returns imposes a shape constraint to obtain a unimodal distribution that is asymmetric and leptokurtic. A mixture model separates trades into time sensitive and time insensitive trades with different intensity functions. The latter group tends to have larger orders and higher returns. The proposed model is compared to B-splines and geometric Brownian motion.

N-H and N-C Bond Dissociation Pathways in Ultraviolet Photodissociation of Dimethylamine.

We investigated the interlinked N-H and N-C photochemistry of primary and secondary amines via the state-resolved detection of vibrationally excited CH3 product and H atom product by 200-235 nm dimethylamine photodissociation using resonance-enhanced multiphoton ionization (REMPI) and velocity map imaging (VMI) techniques. The out-of-plane bending (ν2) vibrationally excited CH3 showed a bimodal translational energy distribution that became unimodal with a near-zero product yield at longer photolysis wavelengths (λphotolysis). In contrast, a unimodal distribution was observed for the C-H stretching (νCH) vibrationally excited CH3 products with an almost constant product yield in the examined λphotolysis region. We ascribed the state-specific energy releases of the CH3 products to two reaction pathways based on calculations of the potential energy surface (PES): the direct N-CH3 dissociation pathway and the indirect N-CH3 dissociation pathway via the N-H bond conical intersection. Meanwhile, the H atom product showed a bimodal energy distribution similar to the ammonia photodissociation model, with an excited-state counterproduct channel that became accessible at a shorter λphotolysis. These results suggest that the N-H and N-C bond dissociations are connected, and these dissociations cause different photochemistry between primary/secondary amines and tertiary amines.

Structural parameters defining distribution of collagen fiber directions in human carotid arteries

Collagen fiber arrangement is decisive for constitutive description of anisotropic mechanical response of arterial wall. In this study, their orientation in human common carotid artery was investigated using polarized light microscopy and an automated algorithm giving more than 4·106 fiber angles per slice. In total 113 slices acquired from 18 arteries taken from 14 cadavers were used for fiber orientation in the circumferential-axial plane. All histograms were approximated with unimodal von Mises distribution to evaluate dominant direction of fibers and their concentration parameter. 10 specimens were analyzed also in circumferential-radial and axial-radial planes (2-4 slices per specimen in each plane); the portion of radially oriented fibers was found insignificant. In the circumferential-axial plane, most specimens showed a pronounced unimodal distribution with angle to circumferential direction μ = 0.7° ± 9.4° and concentration parameter b = 3.4 ± 1.9. Suitability of the unimodal fit was confirmed by high values of coefficient of determination (mean R2 = 0.97, median R2 = 0.99). Differences between media and adventitia layers were not found statistically significant. The results are directly applicable as structural parameters in the GOH constitutive model of arterial wall if the postulated two fiber families are unified into one with circumferential orientation.

Distributional learning of non-native tone contrasts by older adults after training and overnight consolidation

Despite decline in psychoacoustic and statistical learning (SL) abilities, older adults demonstrate remarkably intact perceptual learning in both L2 (tone-word learning) and L1 settings (perceptual adaptation to accented/noise-vocoded speech) but show limited transfer of learning to untrained stimuli. This study tests whether perceptual learning is maintained in an implicit statistical learning task where older adults learn L2 tonal contrasts through exposure to probability distributions of tonal tokens, which may pose higher requirements on both psychoacoustic and SL abilities, and whether sleep-dependent consolidation helps the generalization of perceptual knowledge. L1-Cantonese older adults learned to discriminate a perceptually difficult level-falling tone contrast following a pre-test, training, post- training overnight interval. Training stimuli were synthesized by interpolating naturally produced Mandarin level and high-falling tones into six equidistant steps. Participants either heard a bimodal (two-peak resembling level-falling categories) or unimodal distribution (single-peak) consisting of 256 tokens. ABX discrimination task was administered for testing, with tokens by two genders and on two pseudo-syllables to test generalization. Pilot data of 14 participants showed a trend of group effect with the bimodal group outperforming the unimodal group after training and sleep-dependent consolidation, showing that perceptual learning is maintained in a paradigm that relies heavily on psychoacoustic and SL abilities.

Duration imitation is not mediated by phonological contrast: Evidence from a checked-unchecked tonal contrast in Taiwanese Southern Min

Phonetic imitation is mediated by phonological contrast, as evident in features such as formant, VOT and F0. However, a recent study observed that duration imitation was not mediated by phonological contrast. In contrast to other studies, duration served as a non-primary cue to the phonological contrast in this recent study. This current study further investigates duration imitation in a case where duration serves as the primary cue. We utilized the tonal contrast of T3 versus T33 in Taiwanese Southern Min (TSM), to which duration was identified as the primary cue. We created a seven-step tonal continuum between T3 and T33 by manipulating the tone durations, and recruited seventeen native TSM speakers to imitate each step as closely as they could. The bi- or uni-modality of the distribution of the imitated durations for all seven steps was analyzed using Bayesian regression models. Results showed that the imitated durations were more consistent with an unimodal distribution, suggesting that, unlike other features, the imitation of duration is not mediated by tonal contrast, whether it acts as a primary cue or not. Thus, features exhibit different resistance to phonological mediation in phonetic imitation.

A triple junction energy study using an inclination-dependent anisotropic Monte Carlo Potts grain growth model

This work presents a Monte Carlo Potts grain growth model in which the grain boundary (GB) energies depend on the GB inclination. The inclination is calculated using a linear smoothing approach developed by the authors. In bicrystal simulations with a shrinking grain, the grain changes shape to prefer low energy GB inclinations. However, in polycrystal simulations the preferred inclinations depend on the approach used to assign the triple junction (TJ) energies. Approaches that produce unimodal TJ energy distributions result in the expected behavior of preferring low energy GB inclinations. However, approaches that produce bimodal TJ energy distributions result in medium energy or even high energy inclinations being preferred. Overall, this study underscores the importance of TJs in anisotropic grain growth.

A new tectonic model for the Itmurundy Zone, central Kazakhstan: linking ocean plate stratigraphy, timing of accretion and subduction polarity

The Itmurundy Zone of Central Kazakhstan is a key structure in the core of the Kazakh Orocline representing a typical Pacific-type orogenic belt hosting accretionary complex, ophiolite massifs and serpentinite mélange. The main controversies in the existing tectonic models of the Itmurundy Zone are about the timing of subduction and accretion, the direction and kinematics of subduction and the number of oceanic plates. A new model for the early Paleozoic tectonic story of the Itmurundy Zone is postulated in this paper, based on new detailed geological and U–Pb detrital zircon age data, combined with previously documented geological, U–Pb age, microfossil, geochemical and isotope data from igneous rocks, deep-sea sediments and greywacke sandstones. The present study employs the Ocean Plate Stratigraphy (OPS) model to explain the tectonic processes involved in the evolution of the Itmurundy Zone and to present a holistic story of Ordovician oceanic plate(s), which accretion formed an accretionary complex. The detailed mapping allows distinguishing three types of OPS assemblages: (1) Chert-dominated, (2) OIB-hosting, and (3) MORB-hosting. The U–Pb ages of detrital zircons from sandstones of OIB and Chert types show unimodal distributions with similar main peaks of magmatism at 460–455 Ma in the provenance, and their maximum depositional ages (MDA) span 455–433 Ma. Two samples from OPS Type 3 show the peaks of magmatism both at ca. 460 Ma and the MDA of 452 Ma and 459 Ma, respectively. The MDA of sandstones and microfossils data from chert show the younging of strata to the south and SE in Types 1 and 2 and to NEE for Type 3 (in present coordinates) suggesting double-sided subduction to the NNW and SEE and, accordingly, the co-existence of pieces of two oceanic plates in Ordovician time. The U–Pb zircon data from both igneous and clastic rocks indicate a period of subduction erosion in early Ordovician time. As a whole, the accreted OPS units of the Itmurundy Zone record the timing of subduction and accretion from the early Ordovician to the early Silurian, i.e., 60 Ma at shortest.

Normative percentile ranking best reveals sensorimotor impairments of postural sway in type 2 diabetes

Background Type 2 Diabetes (T2D) is associated with a higher magnitude of static postural sway. This investigation compared three statistical methods to explore the role of sensory modalities contributions to posture in T2D. Research design and methods Two groups were evaluated in this study ( n = 20); T2D group - 10 participants with T2D (age 54.6 ± 11.09 years), comparison group - 10 age/sex matched healthy participants (age 53.18 ± 9.89 years). Postural sway data was collected using the modified Clinical Test of Sensory Integration in Balance (mCTSIB), consisting of four 20-s trials on a balance plate with manipulations of vision and support surface to target the contributions of proprioceptive, visual, and vestibular senses. Scores were assessed by group wise analysis of path length, group wise analysis of percentile rank, and distribution of percentile rank. Results The two-way ANOVA used for the group wise analysis of path length and percentile rank showed significant differences between groups scores ( p < .05), but no significant interactions between group and condition. The frequency distribution of percentile rank of the T2D group revealed unimodal distributions for all conditions except for vestibular, which was found to have the highest and lowest percentile ranks of any condition. Conclusion The results show that the individualized normative analysis revealed aspects of individual impairments that would have otherwise been missed using a group-wise method. Though limited, our findings also suggest that impairments to the vestibular system may be more pronounced but less frequent compared to proprioceptive and visual impairments.

Braided biomimetic PCL grafts for anterior cruciate ligament repair and regeneration

Anterior cruciate ligament (ACL) is a knee joint stabilizer with a limited regeneration capacity mainly because of low cellular content. State-of-the-art procedures are unable to restore the functions of the tissue as demonstrated by limited success rates. Regenerative engineering can offer a solution for restoring the functions of torn/ruptured ligaments provided that biomimetic grafts are available as grafts/scaffolds. However, a model construct to test behavior of cells to better understand the healing mechanism of ACL is still missing. This study, firstly, aimed at creating an injured rabbit ACL model. Then, the injured and healthy ACL tissues were characterized in terms of alignment and diameter distributions of collagen fibrils. Next, polycaprolactone (PCL) grafts were prepared from braided electrospun meshes and were characterized in terms of alignment and diameter distributions of fibers. Finally, biomechanical properties of ACL tissue and mechanical properties of PCL grafts were determined and compared. Findings demonstrated that distributions of the fiber diameters of PCL electrospun grafts were similar to diameter distribution of collagens of healthy and injured rabbit ACL. The novelty of this study relies on the determination of the diameter distribution of collagens of healthy and injured rabbit ACL tissues, and fabrication of PCL grafts with diameter distributions similar to that seen in healthy and injured ACLs. This study is significant because it addresses a worldwide clinical problem associated with millions of patients. The fibrous biomimetic graft designed in this study is different from the traditional grafts that exhibit unimodal distribution, and it is expected to have a significant contribution to ACL regeneration efforts.

Transcending borders: Remarkable genetic homogeneity among Barred mudskipper (Periophthalmus argentilineatus) populations in the southwestern Indian Ocean

AbstractIn the past 24 years, the Southwestern Indian Ocean (SWIO) region has lost about 4% of its mangroves due to unsustainable extraction, land clearance for agriculture and climate change impacts. Since this loss risks fragmenting mangrove fauna, this study analysed 179 D‐loop sequences (329 base pairs) of Barred mudskipper (Periophthalmus argentilineatus) sampled from estuarine mangroves in the SWIO to test two hypotheses: (1) whether Barred mudskipper populations in the region display genetic connectivity along the fragmented mangroves and (2) whether these populations have experienced demographic declines in recent history. The populations showed low and insignificant indices of genetic differentiation (FST = −0.00068, p > 0.05; ΦST = 0.005, p > 0.05), and haplotypes from different localities did not cluster according to their geographical origins. Similarly, Bayesian phylogenetic analysis grouped all SWIO haplotypes into one cluster, suggesting the hypothesis of genetic interconnectedness could not be rejected. Furthermore, the populations showed high haplotype diversity (0.96 ± 0.007) combined with low nucleotide diversity (1.09 ± 0.61%). They also showed significant negative Tajima's D values (−1.71, p < 0.02) and a unimodal mismatch distribution, indicating the hypothesis of demographic expansion post a historical bottleneck could not be rejected. Nevertheless, the Bayesian skyline plot indicated an increase in population size post‐bottleneck, suggesting an ongoing recovery. These findings indicate that, despite varying management approaches in the SWIO, Barred mudskipper populations exhibit genetic exchange that transcends geographical and administrative borders. This implies that their populations should be considered a single stock within the region. These findings underscore the need for coordinated management approaches among the SWIO countries. This is crucial because inconsistencies in conservation measures across borders could disrupt the population's interconnectedness, potentially impacting their genetic integrity and long‐term survival.