Giardia intestinalis (G. intestinalis), a parasitic organism that causes gastrointestinal infections, represents a huge challenge in precisely identifying species from microscopic images. The complexities of accurate diagnosis and treatment require a shift towards automated solutions that enhance diagnostic efficiency and accuracy. In this study, we take advantage of the YOLOv8 deep learning model, comparing its performance with traditional methods, to enhance Giardia intestinalis detection.Our dataset, which has been carefully obtained by Burdur Mehmet Akif Ersoy University Faculty of Veterinary Medicine, Department of Pathology adds a unique dimension to our research. The dataset consists of 264 images of G. İntestinalis and is subjected to preprocessing with RGB/grayscale filters and contrast-limited adaptive histogram equalization for optimal model input.Deep learning architectures tested, including YOLOv8, show an accuracy rate of 95%. Notably, the YOLOv8 model shows promising results, indicating its potential to transform the diagnosis of G. intestinalis. Beyond immediate application, our research paves the way for the integration of YOLOv8 into broader healthcare contexts, promising effective tools for managing G. İntestinalis infections.Furthermore, our study allows the transfer of G. İntestinalis diagnostic expertise from expert veterinarians to the AI model. Veterinarians working in this field can now obtain preliminary diagnostic information through a mobile application. This innovative approach enhances the competence of veterinarians and expands their experience in this field.This research significantly pushes the boundaries in G. İntestinalis image analysis but also puts the foundation for the broader use of advanced deep learning techniques in medical applications. The implications of our findings extend beyond G. İntestinalis diagnosis, providing insight into the transformative impact of YOLOv8 in medical and biological image analysis. Our study opens the way for future developments, shaping the path of intelligent computer vision methods in real-world medical applications.