Underground Sources Research Articles

Trace metals in CO 2 -rich Green River springs, Utah, USA: an analogue for engineered storage

Abstract Sedimentary rocks with high natural CO 2 concentrations provide invaluable analogues for the long-term engineered storage of CO 2 . Some previous studies have reported high trace metal concentrations in sandstone aquifers exposed to CO 2 , a cause for concern should stored CO 2 leak into underground sources of drinking water. However, the intensively studied Jurassic sandstone aquifer in the San Rafael–Green River (Utah, USA) area has trace metal concentrations that are within US Environmental Protection Agency's limits for drinking water. Exceptions are As which is plausibly introduced into the aquifer by saline brines external to the aquifer, and salinity which largely is. This shows that CO 2 in aquifers does not inevitably cause trace metal contamination. CO 2 –water–rock batch experiments elucidated the controls on the trace metal concentrations. After the addition of CO 2 , the experiments reproduce Cu, Cd, Hg, Ni and Zn well, with less good agreement for Cr and Pb although these are still low compared to drinking water standards. Major cations used as fingerprints for mobilization mechanisms suggest that the trace metals are largely derived by desorption, possibly from grain-coating Fe-oxides, rather than by the dissolution of mineral phases. Possible exceptions are Pb and Ni, plus As which is derived from saline brines.

Analysis of groundwater ion abnormality and its cause of centralized drinking water sources in Jieshou City, China

Groundwater provides drinking water to city and rural residents; which is also one of the chief water sources for commercial and agricultural activities in Jieshou City. We collected and analyzed the samples of 18 underground water source wells in Jieshou. We investigated whether the water was of acceptable quality and had characteristics that exceeded the standard. This study was conducted to determine the chemical characteristics of groundwater and abnormally high super-standard ions found in groundwater. The hydrogeological conditions of the study area were analyzed through data collection; through sample collection and sample testing, the characteristics and types of water chemistry were analyzed by means of mathematical statistics analysis and the Piper chart. The genesis of water chemistry was discussed using the Gibbs chart and correlation analysis; the proportional coefficient of ion molar concentration was used to judge the source, origin, and forming process of groundwater chemical composition. The results show that the groundwater is classified as marginally alkaline water, with a composition of Na-HCO3. The cations are mainly Na+, and the anions are mainly HCO3−. According to the Ⅲ water standard of groundwater quality standard and comparing the content of each ion, Na+ and F− are the primary abnormal super-standard ions, and ions and compounds are the main occurrence states. The concentrations of Na+ and F− exceed the standard for class Ⅲ water. There was a positive correlation between the abnormal Na+ and F−, and the concentration of F− increased with the increase in monitoring depth. The causes of abnormal ions were mainly determined by the lithology of the aquifer in the study area, and most of them are fluorine-containing rocks, which are transferred into groundwater through leaching or hydration. The enrichment of Na+ and F− is influenced by the local primary geological setting, hydrochemical type, hydrogeological conditions, pH and artificial activities, and the primary geological setting is the main influencing factor.

Biotesting for the objectivization of the hygienic evaluation of the surface and underground drinking water sources

Introduction. An algorithm for evaluating the hygienic safety of drinking water sources based on biotesting and studying sanitary and chemical indicators has been developed and tested. Materials and methods. The research methods included sanitary and chemical analysis of water samples, as well as biotesting methods using single-celled green alga Chlorella vulgaris Beijer and bull spermatozoa. Results. For experimental approbation of the developed algorithm for determining the hygienic safety of water based on a two-component system of express biotesting and measurement of sanitary and chemical indicators, 6 underground and 5 surface water sources were evaluated. The response of test objects indicating the presence of acute toxicity in water samples taken from surface and underground in many cases correlates with the excess of MPC for individual indicators identified by sanitary and chemical analysis. For two water samples, differences were found between the results of biotesting and sanitary-chemical analysis, and therefore it is necessary to conduct an extended toxicological and chemical assessment to identify the source of toxic effects. Limitations. The two-component test system cannot be used separately without sanitary and chemical analysis of water media. Conclusion. Biotesting can be an addition to sanitary and chemical analyses, showing the presence of toxic substances that are not in the list of mandatory indicators to be determined (according to MR 2.1.4.0176-20 (in Russian)).

Comparative economic analysis of air conditioning system with groundwater source heat pump in general-purpose buildings: A case study for Kayseri

In this study, which aims to reduce energy consumption and dependence on fossil fuels in buildings, the use of an alternative air conditioning system instead of conventional air conditioning systems (CACS), which are widely used in general-purpose public buildings (GPPBs) with high energy consumption, is investigated. A groundwater source heat pump system (GSHPS) was selected as the alternative air conditioning system that exploits an underground water source used in Kayseri province (Turkey). GSHPSs were used both for heating and cooling of a sample GPPB and all costs were determined in detail in the study. In order to compare whether the GSHPS is economical or not, a commonly used CACS, which consisted of a central heating system with natural gas and an air-cooled cooling system was selected. After system components was selected according to the thermal loads of the building, the system costs were determined considering the initial investment cost and the operating costs. Results showed that the proposed GSHPS was more economical than the CACS in terms of initial investment cost and equivalent uniform annual cost by 22.68% and 11.09%, respectively, despite being 3.87% more expensive in terms of total annual operating costs.

Direct Detection of Dark Matter with DarkSide-20k

DarkSide run since mid 2015 a 50-kg-active-mass dual phase Liquid Argon Time Projection Chamber (TPC), filled with low radioactivity argon from an underground source and produced world class results for both the low mass (< 20 GeV/c2) and high mass ( > 100 GeV/c2) direct detection search for dark matter. The next stage of the DarkSide program will be a new generation experiment involving a global collaboration from all the current Argon based experiments. DarkSide-20k, is designed as a 20-tonne fiducial mass dual phase Liquid Argon TPC with SiPM based cryogenic photosensors, and is expected to be free of any instrumental background for an exposure of >100 tonne year. Like its predecessor, DarkSide-20k will be housed at the INFN Gran Sasso (LNGS) underground laboratory, and it is expected to attain a WIMP-nucleon cross section exclusion sensitivity of 7.4 × 10−48 cm2 for a WIMP mass of 1 TeV/c2 in a 200 t yr run. DarkSide-20k will be installed inside a membrane cryostat containing more than 700 t of liquid Argon and be surrounded by an active neutron veto based on a Gd-loaded acrylic shell. The talk will give the latest updates of the ongoing R&D and prototype tests validating the initial design. A subsequent objective, towards the end of the next decade, will be the construction of the ultimate detector, ARGO, with a 300 t fiducial mass to push the sensitivity to the neutrino floor region for high mass WIMPs.

The first data on methane concentrations in degassing griffins of Lake Baskunchak

For the first time, the distribution of methane in the brine and bottom sediments of the Ulan-Blag stream (tributary of Lake Baskunchak), fed by underground degassing springs, as well as directly in Baskunchak Lake at a distance from underground sources. The composition of the main ions in brine, acid-base and redox conditions in bottom sediments have been established. The brine mineralization in Baskunchak Lake is 312 g/dm3, which is 2.1 times higher than the mineralization of the Ulan-Blag stream. The chemical composition of the brine of both stations is classified as chloride type, sodium cationic composition. The concentration of methane in the brine of the studied underground degassing sources in the ravine stream of the Ulan-Blag reaches high values (up to 215 µl/dm3), exceeding by 1–2 orders of magnitude its concentration in the brine of Lake Baskunchak. The low concentrations of methane in the brine of the lake are probably due to its insignificant flow from the bottom sediments into the water due to the presence of a salt crust that prevents gas emission, as well as due to its small concentrations in the upper layers of sediments.

Rain Water Harvesting Technique Implementation for the Chhadvel Korde Village, Maharashtra

Water is one of the most important resources on Earth. In every way, water is crucial to our daily existence. The amount of water that is available in cities is never enough to meet the entire demand. We must rely on water from underground sources because surface water is not readily available. The amount of rainwater that soaks into the subsurface and replenishes ground water has significantly decreased as a result of rapid urbanisation. In this instance, there must be additional supply to satisfy the demand. When it rains, pure rainwater can be gathered "wherever it falls" to instantly replace the water supply. The act of gathering and organising rainwater in a systematic way for later use is referred to as "rainwater harvesting". Two types of rainwater harvesting that could be used in the research region are rooftop and surface runoff harvesting. By adopting a rainwater harvesting system and constructing natural percolation pits, the groundwater level, soil moisture, and soil fertility can all be increased for plantations. Therefore, using this simple strategy typically leads to more vegetation, which raises the aesthetic value. This article examines every rainwater harvesting system established in the Maharashtra district of Sakri in Chhadvel Korde.

Development of technology for reuse of collector trench waters

It is known that water in nature has the property of self-purification. But by our century, the level of water pollution exceeded the norm. As a result, water could not completely eliminate the contaminants contained in its composition. This also defines the problem of solving on self-purification of water. There are various ways to control the quality of water, from which it is possible to list the following: reduce the number of dissolved salts contained in the water or dilute the water, reduce the hardness of the water, reduce the amount of iron contained in it, reduce the number of floating substances hanging in the water, etc. Therefore, in this proposed method, salt underground water sources are used, and the problems of water for use in rural areas, farms, and similar enterprises have been solved. The degree of desalination depends on the content of water. Magnesium cation water is sufficient to separate the gypsum compounds, which are found to be unfavorable for this method. This requires a method and technology that will distinguish only these salts from water. In this article, the process of adding irrigation water to groundwater, treating it, and reusing it for irrigation has been implemented. An experiment was conducted using Faraday's law. The experiment was mainly to remove unwanted salt content from the water through plates and check the quality of the water. As a result, the excess amount of salt in the water was removed, and it was reused and used for irrigation.

Study of radionuclide composition of the drinking water potential sources at the Semipalatinsk nuclear test site.

The contamination degree with artificial radionuclides of the "Degelen" site streams, as well as the wells and boreholes fed from the underground water sources, has been studied. These objects are the potential sources of drinking water at the Semipalatinsk Nuclear test site. Such approach made it possible to identify the most significant radionuclides-contaminants typical for the selected objects, to reveal the nature of contaminants distribution, and to calculate the radionuclide indices for all the sampling points. According to the obtained data, recommendations can be provided on water purification of the studied objects in order to meet the hygienic criteria for drinking water quality.

Optimal organization of dry lands in case study of Uzbekistan

The article showed that the internal land development projects, which include the organization of the territory, along with other measures, will give certain positive results in the rational organization of the use of dry land. Land planning projects developed based on land assessment data create an opportunity to optimize areas of arid lands and crops and justify the economic and technical-organizational aspects of the effective organization of agricultural production in these areas. The results of the conducted scientific research confirmed that in organizing the use of dry arable land in the plain region, first, it is of great importance to search for water sources in these areas, including the study of underground water sources and to positively solve the issues of water extraction by digging artesian wells. Using the data of soil monitoring in dry areas, the soil's natural fertility, its production characteristics, and the possibilities of its use in agriculture were considered to organize the agriculture of the region. Special agrotechnology for soils in dry areas was proposed. In this case, the use of secondary resources available in the republic (low-grade phosphorite, non-traditional agro-ores, various wastes) in field conditions on low-productivity lands (credit score below 40); it was recommended to obtain new organ mineral fertilizers by applying biotechnological methods on farmlands. Organ mineral fertilizers are prepared by composting based on manure and low-grade coal phosphorites in a ratio of 9:1 for 4 months. Other organ mineral fertilizers are made because of Angren low-grade coal and Central Kyzyl-Kum phosphorite flour. These fertilizers have been tested in farm conditions. The use of the proposed technology is resource efficient and solves environmental problems. When applying this technology, soil fertility is restored and improved, the amount of organic matter and organic matter in it increases year by year, the cost of mineral fertilizers is 30-40% and more, and the cost of organic fertilizer is 3-4 times allows to reduce. According to the research results, an additional harvest of 10-12 s/h was obtained from winter wheat.

Assessing Future Flooding Risk in a Coastal Lagoon Using Hydrogeological Approaches and Analysis of the 2021 Flood Event: A Case Study of Tasi-Tolu Lagoon, Dili, Timor-Leste

This study aims to apply a hydrogeological approaches and analysis of the 2021 flood event of Tasi-Tolu Lagoon to achieve four specific goals. Firstly, the study seeks to determine the natural characteristics of the lagoon, which include factors such as size, depth, water quality, and ecosystem composition. Secondly, the influence of precipitation on the water volume in the lagoon will be examined. This analysis involves assessing historical rainfall patterns in the region, as well as the amount and frequency of precipitation during the 2021 flood event. Thirdly, the hydrogeologic and geologic conditions of the lagoon will be evaluated. This involves examining factors such as the type and structure of the soil and bedrock, the presence of aquifers or other underground water sources, and the movement of water through the surrounding landscape. Finally, the study seeks to assess the risk of future flooding in Tasi-Tolu Lagoon, based on the insights gained from the previous analyses. Overall, this study’s goal is to provide a comprehensive understanding of the hydrogeological factors that contribute to flooding in Tasi-Tolu Lagoon. This knowledge could be used to inform flood mitigation strategies or to improve our ability to predict and respond to future flooding events in the region.

Design and creation of local mobile plants for stock watering and sheep wool washing units in Kalmykia

The purpose of the study The article deals with the issues of theoretical and experimental study of water purification processes for mobile watering points based on local underground water supply sources and the creation of a mobile installation with a closed cycle of water purification of wool washing, taking into account the regional characteristics of farms of the Republic of Kalmykia. Groundwater abstraction in the area of animal pens is justified; water purification up to the regulatory requirements for watering. The issues of purification production with the use of chocolate clays as a reagent, deposits of which are available in the northern part of Kalmykia, in the Priergeninsky district; determination of the total income from the practice of increasing the efficiency of farming; mobile purification technologies with the reuse of purified water have been developed.

Comparison of Total Viable Bacteria Counts and in situ Characteristics in Drinking Water Sources in Sagbama Town, Bayelsa State, Nigeria

Before the 21st century, most of Nigeria's rural and coastal communities obtained their drinking water from surface water sources. However, due to development, many coastal towns now get their drinking water from underground sources. Nevertheless, surface water remains a crucial source of drinking water in communities where groundwater is inaccessible. This study evaluated the density of total heterotrophic bacteria and in situ characteristics of drinking water sources in Sagbama town in the Sagbama Local Government Area of Bayelsa State, Nigeria. Triplicate water samples were obtained from five stations for each of the water types (ground and surface water). The water samples were analyzed following standard procedures. The results of ground and surface water ranged from 2.33 to 2.86 (overall mean of 2.52) Log CFU/mL and 2.70 to 3.87 (overall mean of 3.24) Log CFU/mL for total heterotrophic bacteria counts, 7.05 to 7.68 (overall mean 7.40) and 7.06 to 7.31 (overall mean 7.22) for pH, 146.67 to 232.00 (overall mean 169.13) mg/L and 40.23 to 45.60 (overall mean 41.50) mg/L for total dissolved solids, 207.57 to 327.67 (overall mean 239.31)µS/cm and 56.73 to 64.47 (overall mean 58.55) µS/cm for conductivity, and 0.11 ppt and 0.03 ppt (in all the stations) for salinity. The temperatures of both kinds of water were in the range of 26°C. Except for the pH, salinity, and conductivity of groundwater, an analysis of variance reveals no significant differences (p>0.05) across sites for any water type. The overall t-test revealed that all parameters, except pH and temperature, were statistically distinct (p<0.05) between the various water types. According to Pearson’s correlation, conductivity correlates positively with total dissolved solids for both kinds of water. In contrast, salinity correlates positively with conductivity and total dissolved solids for surface water at p< 0.01. Except for total heterotrophic bacteria counts on surfaces, which exceeded World Health Organization standards, other parameters fell within the Standard Organization of Nigeria and World Health Organization guidelines. Therefore, drinking surface water from the research location without first purifying it increases the chance of avoidable ill health consequences.

Comparative Characteristics of Quality Parameters of Waters of the Oka River in Places of Water Intake of Utility and Drinking Water System in Ryazan

INTRODUCTION: Surface water sources play an important role in supply of large cities with water. Their waters are subject to anthropogenic pollution with different chemical compounds which may be significant risk factors for the health of the population. The article presents the results of comparative sanitary-hygienic characteristics of the water quality of the Oka river in places of water intake of utility and drinking water supply system of Ryazan. Being the only surface source of water supply, the Oka river provides 53% of the total water consumption of the Ryazan population, therefore a rational choice of the place of water intake can have a considerable impact on the effectiveness of water conditioning and purification and on the final quality of drinking water.
 AIM: To give a comparative sanitary-hygienic characteristic of the water quality of the Oka river in places of the water intake of the utility and drinking water supply system of Ryazan.
 MATERIALS AND METHODS: A comparative evaluation of the water quality of the Oka river, as a surface source of water supply, was carried out based on the average long-term values of control parameters (organoleptic, microbiological, chemical) and the proportion of samples that do not meet hygienic standards in the sections of three water intakes in Ryazan in 20122019. The average parameters were compared by the method of variance analysis; for paired comparisons, Scheffe and Tamhein criteria were used, taking into account the results of Levene test. The statistical parameters were calculated in SPSS Statistics 19 program.
 RESULTS: The chemical composition of the Oka river water was characterized by the greatest pollution with iron (2+) and aluminum. The water of Oksky and Borkovskoy water intakes was characterized by higher average concentrations of iron (2+) and ammonia, as well as greater contamination with generalized and thermotolerant coliform bacteria, compared with the water of Sokolovsky water intake.
 CONCLUSION: A lower initial quality of the water in Oksky and Borkovskoy water intakes may be due to their location in the 'blind' river course characterized by the processes of eutrophication and slowed down water exchange. High concentrations of iron (2+) may be associated with supply of stream water from the underground sources or with anthropogenic pollution.

Study on the formation mechanism of a radon source during coal spontaneous combustion in a goaf

Surface isotope radon detection technology (SIRDT) is an effective physical exploration technology that provides a solution for detecting hidden underground fire sources. However, because of the inaccessibility of deep underground spaces, current research on the formation mechanism of radon sources during coal spontaneous combustion in a goaf is insufficient, which severely restricts the use of SIRDT to detect such fires. In this study, the quantitative relationship between the coal temperature and radon exhalation rate was revealed through radon exhalation experiments during the low-temperature oxidation of coal, which provides measurement data for the radon source term of coal spontaneous combustion in a goaf. A multi-physical, coupled numerical model of coal spontaneous combustion in a goaf was constructed, and the intrinsic dynamics of radon migration and the formation mechanism of the radon source in the process of coal oxidation were thoroughly investigated. The results show that the radon distribution in a goaf is because of the competition between air leakage and the “porous chimney effect.” When the temperature of the coal was low, air leakage dominated the radon transportation in the goaf, causing radon to accumulate in the area close to the air-return side. When the temperature of the coal increased, the thermal buoyancy and vortex phenomena appeared in the high-temperature area of the goaf, at this point, the “porous chimney effect” took the leading role. The updraft generated by the thermal buoyancy effect rapidly carried the radon to the top of the goaf. The vortex promoted airflow from the surrounding area of coal spontaneous combustion into the interior of the “porous chimney,” which accelerated the process of coal spontaneous combustion and increased the amount of radon transported upward; thus, further intensifying the accumulation of radon at the top of the goaf. This accumulation becomes a source of radon that continues to migrate toward the surface.

Исследование многолетней динамики органолептических рисков здоровью населения волгоградского Заволжья, формируемых подземными водами, с использованием дистанционного зондирования

The paper considers the potential of using remote sensing methods in assessing the risks of olfactory effects in the population regularly drinking water from shallow underground sources vulnerable to climatic factors. The Volgograd Trans-Volga region, located on the territory of the Caspian lowland, is a typical representative of ardidized territories with highly mineralized groundwater. The xerophytic wormwood-fescue-feather-grass and solonetzic vegetation characteristic of the region determines the effectiveness of using the NDMI indicator to assess the degree of influence of soil aridity on the growth of hardness and total iron content in groundwater. A high degree of closeness of correlations between dependent indicators of the sanitary and hygienic quality of groundwater for drinking purposes and NDMI values in five-year dynamics for all groundwater basins (regions) of the North Caspian province was found. The data obtained can be useful in predicting and managing the risks of reflex-olfactory effects in consumers of water from underground sources in the organization of water supply and water treatment. Keywords: ORGANOLEPTIC RISK, NDMI, GROUNDWATER, HYDROGEOCHEMISTRY, ARIIDIZATION, HARDNESS, IRON

UNDERGROUND WATER DESALINATION DEVICE

The article proposes devices for the desalination of water from an underground source, in particular, they mainly concern the provision of environmentally safe water supply to the population with high-quality drinking and technical water.

Research on Underground CO Sources and Abnormal Exceedance in the Tailgate Corner of Low Metamorphic Coal Seam

ABSTRACT To solve the problem of early warning of coal spontaneous combustion (CSC) caused by abnormal continuous CO overrun in the tailgate corner of low metamorphic coal seam, this paper takes 1306 working face of Dananhu No. 1 coal mine in Hami, Xinjiang as an example. Field measurement, experimental research and numerical simulation were used to study the sources of CO in the tailgate corner and its overrun mechanism, and a CO early warning limit calculation method suitable for CSC oxidation of low metamorphic coal seam is proposed. The results show that the ambient temperature oxidation is the most important source during non-production periods. When ambient O2 concentration is ≥7.7%, the coal sample can undergo ambient temperature oxidation to release CO, and the CO production rate has a good quadratic function fitting relationship with the ambient O2 concentration. The average CO production rate of the dissipation zone in the goaf is 3.3 times that of the oxidation zone, and the CO output proportion is 1.1. The CO early warning limit calculated can indicate the phenomenon of CSC oxidation well. The relevant research work is significant for the safety management of CSC in low metamorphic coal seam mining.

Provisión y disposición de agua en 243 ciudades argentinas: Servicios ecosistémicos en el continuo territorio-ecosistema acuático-centro urbano

Cities depend on different ecosystems that provide them with services. In the case of water supply and effluent disposal in Argentine cities, its recognition is still incomplete and fragmented. Here is presented a first database that maps and classifies the water supply sources and effluent destinations of all Argentine cities >20000 inhabitants (in 2010). Utilizing existing reports, journalistic news and satellite information, water intake and discharge points were recorded, along with other associated infrastructure in 243 cities. It was found that 30.5, 17.5 and 12.6% of the population is exclusively supplied by large transboundary rivers, groundwater and internal rivers and streams, respectively, with 25.2% supplied by mixed sources. The cities that are connected to the public sanitation networks resort mainly to internal rivers and streams (26.6%) for their disposal; however, of the urban population that is not connected to the sewage network, a high proportion discharges into free aquifers. Most Argentine cities do not return sewage water to the same system that supplies it, predominantly transfers from underground sources to internal rivers and streams. The database evidences the contrasting ‘effluent pressure’ (population/flow rate of the receiving system) between cities linked to large rivers (e.g., Paraná basin, 273 inhabitants.m-3.s-1) and internal basins (e.g., Mar Chiquita, 16141 inhabitants.m-3.s-1). This database, open for consultation and updating, makes visible the spatial and hydrological connection between cities and the hydrographic network, helping to improve water security and prioritize efforts to protect aquatic ecosystems that guarantee it from all levels of management.

Contamination of biomedia with N-nitrosamines as a factor in the development of disorders of the immune status in schoolchildren with dyspepsia under conditions of altered polymorphism of candidate genes

Introduction. Contamination of drinking water sources with N-nitrosamines, substances of the first class of danger, creates conditions for the development of disorders of the immune profile in primary school-age children with diseases of the digestive system. Bio- and toxicokinetic features of these substances, when they enter the body with water, form changes on the part of the immune system, and the biodegradation of these substances by enzymes of the first phase of detoxification of the CYP450 family is accompanied by the formation of direct carcinogens capable of DNA methylation, which is characterized by dyspepsia, uncontrolled cell proliferation in the conditions of the CC genotype of the gene of the detoxification enzyme CYP1A1_3 rs4646421. Materials and methods. The study involved fifty two children of primary school age (6-12 years old) living in the Perm Territory, consuming water from underground sources characterized by pollution with N-nitrosamines. The observation group included 30 children with pathology of the digestive system. The comparison group consisted of 22 conditionally healthy children. Chemical (NDMA, NDEA), immunological (IgE specific to NDMA, IgM, CD95+, IL 10, Bcl, cortisol, CA 72-4), and genetic (gene of the detoxification enzyme CYP1A1_3 rs4646421) studies of the biological environment were performed for all children. Results. According to the results of the conducted studies, it was found that the observation group relative to the comparison group was characterized by an increased content of N-nitrosamines in the blood up to 3.8 times; altered immune status in the form of an increase in specific sensitization to NDMA by 1.2 times, inhibition of apoptotic activity of CD95+ markers by 1.8 times (p<0.05) and Bcl2 by 1.3 times, a decrease in the level of expression of the anti-inflammatory cytokine IL10 by 2.3 times (p<0.05), an increase in the expression of the stress hormone cortisol by 1.3 times (p<0.05) and the cancer marker CA 72-4 by 2.5 times; an increase in the frequency of the wild homozygous CC genotype of the CYP1A1_3 rs4646421 gene by 1.5 times, which acted as a risk factor for dyspepsia in conditions of blood contamination with N-nitrosamines (OR=4.5; CI: 1.16-17.40, p<0.05). Limitations. The limitation lies in the use of the identified markers for the diagnosis of immune disorders manifested by blood contamination with nitrosamines in a specific area. Conclusion. The obtained results indicate to an imbalance of the immune profile in conditions of low-level chronic oral intake of N-nitrosamines with a genetic predisposition to the development of toxic effects. So far, the detected violations should be attributed to early signs indicating the formation of dyspepsia in schoolchildren consuming water from underground sources contaminated with N-nitrosamines.