Ultrasonic Extraction Technique Research Articles

Penerapan Ultrasonik dalam Penelitian Aktivitas Antibakteri Staphylococcus epidermidis Ekstrak Daun Kelor (Moringa oleifera): Pendekatan Berbasis Sains dan Nilai Islam

This study reveals the potential of ultrasonic extraction method in yielding valuable extracts from Moringa oleifera leaves. Moringa oleifera, a versatile plant, takes center stage in this research. The concept aligned with the teachings of the Quran regarding the intricacies of Moringa and its application in acne treatment offers a secure and sustainable option. The utilization of ultrasonic extraction technique contributes to enhancing the benefits obtainable from Moringa. The antibacterial properties identified within Moringa position it as a promising candidate within the realm of medical applications, thereby aligning with the values propagated by the Quran, emphasizing the appreciation of nature's wonders.The methodology of this research involves ultrasonic extraction method and phytochemical analysis conducted in a laboratory setting, alongside evaluating the antibacterial potential through literature review. A bibliometric approach is employed to analyze relevant literature and Quranic verses concerning Moringa. This research falls within the qualitative research category and embodies the post-positivist perspective. Findings of the study indicate that the ultrasonic extraction method successfully yields approximately 22.22% of extract. Moreover, the phytochemical analysis reveals the presence of compounds such as flavonoids, saponins, tannins, alkaloids, triterpenoids, and steroids in the ethanol extract of Moringa leaves.

Recovery of phenolic compounds from peach pomace using conventional solvent extraction and different emerging techniques.

The study compared high-pressure, microwave, ultrasonic, and traditional extraction techniques. The following extraction conditions were implemented: microwave-assisted extraction (MAE) at 900W power for durations of 30, 60, and 90s; ultrasonic-assisted extraction (UAE) at 100% amplitude for periods of 5, 10, and 15min; and high-pressure processing (HPP) at pressures of 400 and 500MPa for durations of 1, 5, and 10min. The highest yield in terms of total phenolic content (PC) was obtained in UAE with a value of 45.13±1.09mg gallic acid equivalent (GAE)/100g fresh weight (FW). The highest PC content was determined using HPP-500MPa for 10min, resulting in 40mg GAE/100g, and MAE for 90s, yielding 34.40mg GAE/100g FW. The highest value of antioxidant activity (AA) was obtained by UAE in 51.9%±0.71%. The PCs were identified through the utilization of Fourier transform infrared (FTIR) spectroscopy and high-performance liquid chromatography (HPLC). Utilizing multivariate analysis, the construction of chemometric models were executed to predict AA or total PC of the extracts, leveraging the information from IR spectra. The FTIR spectrum revealed bands associated with apigenin, and the application of HPP resulted in concentrations of 5.41±0.25mg/100g FW for apigenin and 1.30±0.15mg/100g FW for protocatechuic acid. Furthermore, HPLC analysis detected the presence of protocatechuic acid, caffeic acid, p-coumaric acid, and apigenin in both green extraction methods and the classical method. Apigenin emerged as the predominant phenolic compound in peach extracts. The highest concentrations of apigenin, p-coumaric acid, and protocatechuic acid were observed under HPP treatment, measuring 5.41±0.25, 0.21±0.04, and 1.30±0.15mg/kg FW, respectively.

A modified strategy to improve the dissolution of flavonoids from Artemisiae Argyi Folium using ultrasonic-assisted enzyme-deep eutectic solvent system

In this study, enzyme-deep eutectic solvent-assisted ultrasonic extraction technique (EnDUE) was developed for the efficient dissolution of flavonoids from Artemisiae Argyi Folium. The extraction results of Artemisiae Argyi Folium flavonoids (quercetin, luteolin, and isorhamnetin) were used as indicators to investigate the influencing factors through single factor experiment, Placket-burman design, and Box-behnken design, so as to obtain satisfactory yields. After systematic optimization, the optimal conditions for extraction of the target flavonoids were: Choline chloride/1,4-butanediol with a water content of 25%, cellulase+pectinase with a concentration of 1.6%, solid-liquid ratio of 1/32 g/mL, pH of 4.2, ultrasonic frequency of 80 kHz, ultrasonic power of 160 W, ultrasonic temperature of 40 °C, and ultrasonic time of 25 min, respectively, which derived a total yield of 8.06 ± 0.29 mg/g. Compared with the reference techniques, the proposed EnDUE technique showed significant advantages in the yield and extraction efficiency of flavonoids. In addition, after preliminary purification, the Artemisiae Argyi Folium flavonoids showed good antioxidant activity. Deep eutectic solvent (DES) can degrade the cell wall components and increase the action site of enzyme, and enzyme can promote the penetration of DES into the cell wall matrix, which is mutually beneficial to the dissolution of intracellular components. Therefore, the extraction technique proposed in this work (EnDUE) greatly promotes the dissolution of flavonoids from Artemisiae Argyi Folium, and provides theoretical support for the further application of plant flavonoids.

Study of the antioxidant and anti-pancreatic cancer activities of Anchusa strigosa aqueous extracts obtained by maceration and ultrasonic extraction techniques.

Pancreatic cancer is a highly aggressive malignancy and a leading cause of cancer-related deaths worldwide. Moreover, the incidence and mortality rates for pancreatic cancer are projected to keep increasing. A major challenge in the treatment of pancreatic cancer is the lack of effective screening approaches, which contributes to its poor prognosis, indicating the need for new treatment regimens and alternative therapies, such as herbal medicine. The medicinal plant A. strigosa, which is widely distributed in the Eastern Mediterranean region, is a short prickly plant from the Boraginaceae family that has been widely used in traditional medicine for treating various diseases. Nevertheless, its effect on human pancreatic cancer remains poorly investigated. In the present study, we screened the phytochemical content of Anchusa strigosa aqueous extracts obtained by maceration and ultrasound-assisted methods (ASM and ASU, respectively) and evaluated their antioxidant effects. We also investigated their anticancer effects and possible underlying mechanisms. The results show that both extracts were rich in bioactive molecules, with slight differences in their composition. Both extracts exhibited remarkable antioxidant potential and potent radical-scavenging activity in vitro. Additionally, non-cytotoxic concentrations of both extracts attenuated cell proliferation in a time- and concentration-dependent manner, which was associated with a decrease in the proliferation marker Ki67 and an induction of the intrinsic apoptotic pathway. Furthermore, the extracts increased the aggregation of pancreatic cancer cells and reduced their migratory potential, with a concomitant downregulation of integrin β1. Finally, we showed that the ASM extract caused a significant decrease in the levels of COX-2, an enzyme that has been linked to inflammation, carcinogenesis, tumor progression, and metastasis. Taken together, our findings provide evidence that A. strigosa extracts, particularly the extract obtained using the maceration method, have a potential anticancer effect and may represent a new resource for the design of novel drugs against pancreatic cancer.

Ultrasonic-assisted sustainable extraction and dyeing of organic cotton fabric using natural dyes from Dillenia indica leaf

As a means of preventing environmental damage caused by synthetic dyes, eco-friendly textile dyeing with natural dyes is gaining popularity worldwide. This study focused on the extraction of dyes from the leaf of Dillenia indica (D. indica) tree using an ultrasonic extraction technique and applied on the organic cotton fabrics. The ultrasonic method was used for both extractions of D. indica dyes and dyeing of organic cotton fabrics. Here, the amount of D. indica powder used were 5% and 6.67% for producing light and dark shade, respectively. The investigation of the color fastness to washing, rubbing, and light for the dyed organic cotton fabrics indicated an excellent rating. The spectrophotometric analysis revealed the L* (lightness or darkness), a* (redness or greenness), b* (yellowness or blueness), C* (chroma), h* (hue), R% (reflectance), and K/S (color strength) values, which accurately represented the shade of the dyed organic cotton fabric. To understand the interaction between D. indica dye and organic cotton fabrics, different characterization including, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were performed. The characterization outcomes confirmed the successful deposition of D. indica dyes on the organic cotton fabrics. The other comparable testing results such as bursting strength, air permeability, and thermogravimetric analysis (TGA) of dyed and undyed organic cotton fabrics were in the acceptable range. One of the important findings of this research was no chemicals were utilized during the extraction and dyeing of organic cotton fabrics. This process can be referred to as completely chemical-free and advantageous for the environment because no chemicals were needed during extraction or dyeing. Therefore, the natural dye extracted from D. indica is extremely promising and could be a viable option for the sustainable dyeing of cotton fabrics in the textile dyeing industry.

Eco-Friendly Dyeing of Tencel Fabric by Using Natural Dye Extracted from Eucalyptus Leaves

The usage of natural dyes as environmentally suitable dyeing methods has extended during the recent years. The prime focus of this research is on the extraction and purification of natural dyes from eucalyptus leaves. With its numerous applications, eucalyptus leaves are highly valued for their diverse application, particularly as a rich source of natural colours. Quercetin is the primary colouring component in eucalyptus leaves. It has been utilized as a food dye with strong antioxidant properties and as an antioxidant. The goal of this research is to dye Tencel fabric using eucalyptus leaf-derived natural dye. The dye was extracted by using an ultrasonic extraction technique. Different ratios of the two mordants copper sulfate and ferrous sulphate were applied. Dyeing was carried out with different temperatures and concentrations and the pre-mordanting method was used for mordanting. The dyed Tencel fabric displayed a good colour strength of (K/S = 4.72), CIELAB values, and good to excellent light fastness (5), wash (4-5), perspiration (4-5), and rubbing (dry and wet = 4-5) fastness properties.

Formulation and Nutritional Characterization of Mustard and Sesame Oilseed Cake Extract-Based Functional Drinks

The current investigation was carried out to develop polyphenol-enriched functional drinks from oil industry waste. Purposely, polyphenols were extracted from the mustard and sesame oilseed cakes through ultrasound-aided extraction alongside conventional extraction mode for comparison purposes. Among the oilseed cake extracts, sesame with methanol and ultrasonic extraction exhibited best results for TPC, DPPH, FRAP, ABTS, and β-carotene as 39 ± 0.04 g GAE/100 g, 35 ± 0.02 g TE/100 g, 20 ± 0.02 g TE/100 g, 18 ± 0.03 g TE/100 g, and 35 ± 0.05 g TE/100 g, respectively, and mustard showed 31 ± 0.04 g GAE/100 g, 20 ± 0.01 g TE/100 g, 16 ± 0.02 g TE/100 g, 12 ± 0.01 g TE/100 g, and 30 ± 0.05 g TE/100 g, respectively. In case of conventional extraction and methanol as solvent, sesame revealed outcomes for TPC, DPPH, FRAP, ABTS, and β-carotene as 13 ± 0.02 g GAE/100 g, 17 ± 0.03 g TE/100 g, 10 ± 0.01 g TE/100 g, 21 ± 0.04 g TE/100 g, and 15 ± 0.03 g TE/100 g, respectively, compared to mustard which showed for TPC, DPPH, FRAP, ABTS, and β-carotene as 11 ± 0.02 , 12 ± 0.01 , 08 ± 0.01 , 17 ± 0.03 , and 10 ± 0.01 , respectively. Likewise, for mustard oilseed cake extract with conventional extraction technique and water as solvent, minimum findings were observed for TPC, DPPH, FRAP, ABTS, and β-carotene as 07 ± 0.01 , 09 ± 0.02 , 06 ± 0.01 , 14 ± 0.03 , and 07 ± 0.01 , respectively. T0 without extracts, T1 (sesame oilseed cake extract based Functional drink) , and T2 (mustard oilseed cake extract based Functional drink). The recorded values for total phenols, total flavonoids, total carotenoids, and vitamin C in T0, T1, and T2 were 29.79 ± 6.05 , 32.53 ± 7.05 , and 30.5 ± 5.05 ; 26.33 ± 5.05 , 30.60 ± 7.05 , and 29.75 ± 5.05 ; 2.11 ± 0.05 , 2.12 ± 0.05 , and 2.08 ± 0.01 ; and 31.7 ± 7.05 , 30.5 ± 5.05 , and 29.6 ± 6.05 , respectively. Likewise, sensory evaluation for color, flavor, sweetness, sourness, and overall acceptability during 2 months of storage depicted acceptable scores. The inclusive best outcomes for phytochemical analysis were achieved with sesame oilseed cake extracts by applying ultrasonic extraction technique and methanol as solvent. In the same way, among the developed functional drinks, T1 (sesame oilseed cake extract-based functional drink) exhibited best physiochemical as well as storage characteristics.

Optimization and kinetic studies of d-galacturonic acid extraction from basil seed using different extraction techniques

This study presents d-galacturonic acid extraction from basil seeds using stirred reactor extraction, conventional soxhlet extraction, and ultrasonic extraction techniques. Different process parameters such as type of solvent, pH (2–9.5), solid-to-solvent ratio (0.5:100–2:100), time(10–70 min), temperature (40–90 °C), and agitation speed (100-700RPM) were optimized. The yield of D-gala using ultrasound-assisted extraction (104.25 mg/g of basil seeds) was higher as compared to the stirred reactor (92.64 mg/g of basil seeds) and soxhlet extraction (52.2 mg/g of basil seeds). Moreover, the time required for extraction using ultrasound was significantly less (15 min) as compared to the stirred reactor (60 min) and soxhlet extraction (280 min). The process effectiveness in terms of yield of D-gala per unit energy dissipated in ultrasound-assisted extraction (12.46 mg/g of basil seed kJ) was around 13 times greater than stirred reactor (0.975 mg/g of basil seed kJ) and about 63 times greater than soxhlet extraction (0.201 mg/g of basil seed kJ). As a result, ultrasonic extraction is regarded as the best alternative approach to the stirred reactor and soxhlet extraction for getting a higher d-galacturonic acid yield from basil seeds. An empirical kinetic model of second order rate equation and a mathematical model using a nonlinear regression technique was investigated for the process, which shows numerical correlation and significance factor between the yield and all other parameters. The detailed characterization of the extracted components has been carried out using Fourier transform infrared spectroscopy (FTIR), Nuclear magnetic resonance (NMR), and Liquid chromatography and mass spectroscopy (LCMS).

Mechanisms affecting the biopharmacological properties of saffron active ingredients based on anti-inflammatory activity

ABSTRACT To explore the mechanisms affecting the biopharmacological properties of the active components of saffron. Ultrasonic extraction technique was applied to extract the active components from safflower materials and combined with molecular docking technique to screen four safflower anti-inflammatory active components, naiophenol-3–0-rutinoside, kaempferol-3-O-glucoside, quercetin and lignan. In order to understand the mechanism of the influence of its biopharmaceutical properties, the changes of the solubility and permeability parameters of the anti-inflammatory active components of safflower were obtained by using the classical shaking bottle method and the body enteric irrigation method. The compound environment had a great influence on the solubility of single active ingredient. The calculated results of the dose number of all ingredients were greater than 1, and the solubility properties were all high solubility. The absorption parameters and osmotic coefficients of kaempferol-3-O-glucoside and lignan varied insignificantly under different concentration conditions, while each osmotic coefficient of kaempferol-3-O-rutinoside and quercetin showed a positive proportional decreasing trend. In addition, the osmotic coefficients of the three components except kaempferol-3-O-glucoside did not change with environmental changes, and the overall anti-inflammatory active ingredient absorption mechanisms included both passive diffusion and active transport. The two biopharmaceutical properties of the solubility and permeability of the active ingredients of Safflower can be changed under the influence of the application environment and drug concentration. In the pharmaceutical process, the preparation means can be optimized from this aspect to achieve the purpose of improving the clinical efficacy of Safflower anti-inflammatory drugs.

Valorization of mustard and sesame oilseed cakes for food application through eco-innovative technologies.

Agricultural waste valorization is currently getting attention across the world owing to its environmental impact and rich phytochemistry. The mandate of the current investigation was the extraction and characterization of bioactive moieties from the mustard oilseed cake/meal MOC and sesame oilseed cake/meal SOC through ultrasound extraction (UE) techniques due to its higher yield and less burden on the environment as compared to conventional extraction (CE). Purposely, the MOC and SOC were initially subjected to compositional analysis. Thereafter, bioactive moieties were extracted by using different solvents, that is, ethanol and distilled water, and by applying conventional and ultrasonic extraction techniques. The outcomes indicated that among the techniques, ultrasound exhibited the highest results, and in solvents, ethanol performed better. The treatment extracted with ethanol with UE at 10min showed the best result for total phenolic contents (TPC) as (6.07 ± 0.03 09 g GAE/100 g MOC) and (7.09 ± 0.04 g GAE/100 g SOC), DPPH radical scavenging activity (67.3 ± 1.9 TE/100 g MOC) & (72.68 ± 1.9 TE/100 g SOC), and FRAP was recorded as (2.83 ± 0.02 g TE/100 g MOC) & (3.56 ± 0.03 g TE/100 g SOC). The higher antioxidant potential showed that the mustard and sesame waste holds significant therapeutic potential owing to its rich antioxidant profile and thus should be utilized for the development of functional products against lifestyle-related disorders. In conclusion, ultrasound is a better technique for maximum as well as accurate extraction, with ethanol exhibiting as a better solvent for this process with more yields as compared to distilled water.

EFFECTS OF DIFFERENT PARAMETERS ON THE LIPOLYTIC ACTIVITIES OF LIPASE AND PHOSPHOLIPASE EXTRACTED FROM PRUNUS ARMINICA MEAL OF HUNZA CULTIVAR


 
 
 
 Prunus Arminica.L seeds of Hunza cultivar were defatted with chloroform, methanol mixture (2:1v/v) by ultrasonic assisted extraction technique. The lipase and phospholipase extracted, incubated at 400C and pH 6 in citrate buffer for 1 hour. The lipolytic activities of enzymes were measured at different temperatures i,e 30-700C , pH i,e 3-9 and solvents i,e n-heptane , Di-isopropyl ether and Cyclo hexane. 10 % emulsion of lecithin and olive oil used as the substrate for phospholipase and lipase substrates respectively. The enzymes showed stability at lower temperatures i,e 35-500C and slightly acidic to neutral i.e pH 5 to pH 7. Concentration of liberated fatty acids was the indicator of the enzymes activity which were measured by taking absorbance at 440nm. Stearic acid with different concentrations in hexane and methanol (1:1v/v) were used for the standard curve. Cu-TEA reagent with small quantity of CaCl2 was used for maximum extraction of Cu-FFA and for stable value of blanks in the present study. The enzymes showed maximum activities at 450 C temperature and 6 pH.
 
 
 

Effect of combined ultrasonic and enzymatic extraction technique on the quality of noni (Morinda citrifolia L.) juice.

In order to obtain noni juice with high yield and good quality, the effect of combined extraction technique of enzymatic treatment (EZ) and ultrasonication (US) on the overall quality of noni juice was investigated. Moreover, the extraction performance of the EZ-US combined extraction technique was compared with that of EZ-based extraction and the US-based extraction. Response surface methodology (RSM) was designed to optimize the parameters of ultrasonic treatment, by taking consideration of the extraction efficiency, quality parameters and bioactive ingredients of noni juice. The results indicated that combined ultrasonic and enzymatic treatment achieved a synergistic effect on promoting the quality of noni juice. The maximum juice yield of 67.95% was obtained under ultrasonication for 10min at 600W after enzymatic treatment (EZU). In addition, EZU-treated juice exhibited the highest contents of total phenolic and flavonoid, which were 148.19±2.53mg gallic acid/100mL and 47.19±1.22mg rutin/100mL, respectively, thus contributing to better antioxidant activity. Moreover, the EZU treatment significantly reduced the particle size of noni juice, and improved its suspension stability and rheological properties. FTIR results indicated that the treatments did not bring major changes in the chemical structure and the functional groups of compounds in noni juice. Therefore, EZU treatment can be successfully applied to the extraction of noni juice with better nutritional properties and overall quality.

In vitro antibacterial and antioxidant activities of cotton fabrics treated with bael fruit shell extract

The present study is aimed at evaluating the antibacterial and antioxidant properties of bael fruit shell (BFS) extract. Hot water extraction (HWE) and ultrasonic assisted extraction (UAE) techniques have been used to understand the effectiveness of the extraction process and its relation to impart enhanced functional property of cotton fabric. The cotton fabric has been treated with BFS extract by using padding mangle. The effectiveness of antibacterial activity against both the Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and antioxidant property has been evaluated both qualitatively and quantitatively. The results reveal that UAE-BFS treated fabric shows 93% reduction for E coli and 82% for S aureus, which is higher as compared to HWE-BFS treated fabric (91% for E coli and 61 % for S aureus). This trend has also been observed in qualitative zone of inhibition method. Antioxidant efficacy of UAE treated fabric is 86%, whereas HWE treated fabric registers 80% activity.

Manipulation of the Phenolic Quality of Assam Green Tea through Thermal Regulation and Utilization of Microwave and Ultrasonic Extraction Techniques

The aim of this study was to investigate the catechin levels and antioxidant activities as manipulated by roasting temperature and roasting time of green tea. Roasting temperature and time varied between 100–300 °C and 60–240 s in green tea production. The main interactions measured were effects on the antioxidant activities, total phenolic content, DPPH, ABTS, FRAP and catechin content (catechin (C), epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG) and epicatechin (EC)). Optimum roasting conditions were determined as 270 °C for 240 s, since this enabled high catechin contents, antioxidant activities and production yield. The extraction methods for green tea including traditional extraction (TDE), microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) using 60% ethanol as solvent were investigated to evaluate the highest bioactive compound and yield of extraction. MAE was found to be more efficient in green tea extraction compared to UAE and TDE. The extracts showed significant cytotoxic potential against the Huh-7 cell line, in concentrations ranging from 31.25 to 1000 µg/mL. The results are useful in understanding the relationship between thermal treatment and extraction conditions on the chemical and nutritional properties of tea catechins, making it possible to select the production and extraction conditions that maximize the levels of beneficial tea ingredients.

Edible Green Solvent for Optimized Catechins Extraction from Green Tea Leaves: Anti-Hypercholesterolemia.

Catechin polyphenols are the major bioactive ingredients in green tea with various human health benefits. Extraction of catechins from green tea (GTE) leaves at optimized standard conditions is still a challenging approach. An optimized, rapid, and economic extraction method is industrially needed. We hypothesized that certain extraction techniques in the presence of natural polymers and antioxidants might improve GTE catechin extraction yield and its biological activity. The effect of microwave (30–60 seconds irradiation in a typical kitchen microwave) assisted extraction (MAE) and ultrasonic assisted extraction (UAE) techniques were evaluated separately and in combination. To study the effect of the extraction solvent, nine edible green solvent combinations were investigated namely water, ascorbic acid, chitosan/ascorbic acid, carboxymethylcellulose /ascorbic acid, methylcellulose /ascorbic acid, chitosan/methylcellulose/ascorbic acid, methylcellulose, chitosan/acetic acid, and ethanol. The amounts of extracted catechins from green tea leaves were quantified with HPLC-UV.Data showed that the use of MAE & UAE technique was the optimal in producing a higher extraction yield of catechins. Chitosan/ascorbic acid was the optimized solvent with high extraction efficiencies of catechins. Studies in high fat diet fed animals demonstrated significant reduction of total cholesterol and LDL-C by GTE after 3 weeks of oral daily administration. In conclusion, efficient extraction, and stabilization of catechins from green tea leaves demonstrated a significant lowering of high fat diet-mediated elevation in blood cholesterol and LDL-C levels.

New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

In this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

Simultaneous determination of borax and polyphosphates content in food by liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS)

A new method based on LC-ICP-MS technique has been developed and validated for&amp;nbsp;the simultaneous determination of borax and polyphosphates (phosphate, pyrophosphate,&amp;nbsp;tripolyphosphate) in foods. The analytes were extracted from the samples with 18 mM&amp;nbsp;sulfuric acid solution using ultrasonic vibration extraction technique before being filtered and&amp;nbsp;analyzed by LC-ICP-MS system. The method was validated with calibration curves that have&amp;nbsp;the coefficient of determination (R2) higher than 0.995. The method limits of detection (LOD)&amp;nbsp;of borax, phosphate, pyrophosphate (Pyro-P), and tripolyphosphate (Tripoly-P) were in the&amp;nbsp;range of 2.5 to 15.0 mg/kg. The recoveries were in the range of 86.0 to 107.0% and the relative&amp;nbsp;standard deviations were below 5.23%. A total of 83 samples of cereal, meat, and seafood-based&amp;nbsp;food were analyzed for research purposes. The results did not indicate a risk of using borax&amp;nbsp;and polyphosphates in most rice products. Meanwhile, meat products including pork paste and&amp;nbsp;roasted pork sold in traditional markets had a borax detection rate of over 60%. This study&amp;nbsp;showed that there was still a risk of using borax in food processing and the use of polyphosphates&amp;nbsp;in food products collected in some districts in Hanoi city.

Development and antioxidant characterization of Ginger-Mint drink prepared through different extraction techniques

Mint and ginger are rich sources of phenolic compounds, and thus the present study aimed to assess the impact of different extraction techniques on their polyphenolic yield and antioxidant activity, and to develop a ginger-mint drink. Conventional, ultrasonic and supercritical fluid extraction techniques were used, and ethanol, methanol and distilled water selected as extraction solvents for mint and ginger. Antioxidant activity was determined by β-carotene bleaching inhibition, DPPH and FRAP assays. Total phenolics (TPC) and flavonoids contents were also quantified, and physicochemical and sensory characteristics of the ginger-mint drink assessed. The results elucidated higher TPC, β-carotene bleaching inhibition, DPPH, FRAP and total flavonoids of mint and ginger in supercritical on 30 min as 2481.6 ± 245 and 2441 ± 240, 74 ± 7.1 and 69 ± 6.1, 79 ± 7.1 and 74 ± 6.1, 17.21 ± 1.3 and 16.4 ± 1.5, and 35 ± 2.8 and 34 ± 2.9, respectively, followed by ultrasonic assisted extraction. Likewise, the functional drink prepared from these extracts exhibited promising physicochemical, sensorial and antioxidant traits. In conclusion, supercritical fluid extraction was the best method for maximum and accurate extraction with ethanol revealing to be the best solvent for this process, with higher yields, when compared to methanol and distilled water.

Antimicrobial, Antioxidant and Cytotoxic Activities of Cosmos Caudatus extracts

This study aims to investigate the in-vitro antimicrobial potential of Cosmos caudatus against pathogenic bacteria, its antioxidant activity and cytotoxic effect on cancer cell lines. In vitro antimicrobial activities of ethanol and water extracts of Cosmos caudatus herb against pathogenic bacteria, Staphylococcus aureus, Salmonella sp., Pseudomonas aeruginosa and Escherichia coli were tested by agar well diffusion. Antioxidant properties of the extracts was evaluated by 2,2-diphenyl-1-picryl-hydrazyl radical scavenging activity method. Additionally, cytotoxic activity of the extracts on HCT 116 colorectal carcinoma cell line was determined by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Vero cells were used as a control. The quantification of these extracts showed that this plant is rich in secondary metabolites. Moreover, the conventional soxhlet extraction technique was more effective than maceration and ultrasonic assisted extraction technique. In antioxidants assay, both the ethanolic and water extracts through ultrasonic extraction method exhibited the highest DPPH free radical scavenging capacity compared to maceration and sohxlet extraction method. In antimicrobial assay, both the water and ethanol extracts showed no inhibitory action against the tested pathogenic bacteria. For anticancer activity, the F2, F7 and F8 fractions showed potent dose dependent cytotoxicity on Human Colorectal Carcinoma Cells with IC50 value of 15.53 ± 0.4, 32.72 ± 0.3 and 34.16 ± 1.4 μg/mL, respectively.

Occurrence of polycyclic aromatic hydrocarbons in water and sediment samples from KwaZulu Natal Province, South Africa

AbstractSolid‐phase extraction (SPE) and ultrasonic extraction (UE) techniques followed by Gas Chromatography‐Mass Spectrometry (GC‐MS) have been modified for qualitative and quantitative analysis of polycyclic aromatic hydrocarbons (PAHs) in water and sediment samples. Percentage recoveries of PAHs ranged from 85 to 121% in water and 82 to 117% in sediment samples. The limits of detection (LOD) and limits of quantification (LOQ) ranged from 0.02 to 0.2 and 0.05 to 0.5 µg/L for SPE while for UE, they were between 0.008–0.09 and 0.02–0.30 µg/kg, respectively. The concentration levels of PAHs (naphthalene, acenaphthene, acenaphthylene, fluorine, anthracene, phenanthrene and pyrene) detected in water samples were 0.071–2.7, 2.0–10.4 and 2.5–3.5 µg/L in wastewater, river water, and dam water, respectively. In sediment samples, concentration levels of PAHs were between 2.8–42.0 and 2.8–3.9 µg/kg, in river and dam sediment, respectively.