Ultrafine Bubble Water Research Articles

Research of Cleaning by Using Ultra-fine Bubble Water

Research of Cleaning by Using Ultra-fine Bubble Water

Effect of ultrafine bubbles water on seed germination

Ultrafine Bubbles (UFBs) technology is a new and emerging science, capable of infusing UFB in liquids for long periods of time. UFBs is extremely small gas bubbles in liquid with diameter less than 10−6 m. This paper discussed the application of ultrafine bubbles water for seed germination. Seed germination is the process from seed to a sprout in which the metabolic process of a plant gets activated after a period of dormancy. A high seed germination percentage is important for vegetable production. Comparison experiments of seed germination rate were performed using distilled water and water containing ultrafine bubbles. This experiment utilized the dissolved oxygen concentrations of distilled water with approximately 10 and 20ppm. The result indicates that the application of ultrafine bubbles water had a positive effect on the germination rate of seeds.

Ozone ultrafine bubble water induces the cellular signaling involved in oxidative stress responses in human periodontal ligament fibroblasts

ABSTRACT Periodontitis is a chronic inflammatory disease caused by oral microorganisms in the subgingival biofilm. Stable aqueous ozone ultrafine bubble water (OUFBW) has recently begun to be used as an antiseptic in the treatment of periodontitis. The effectiveness of OUFBW is thought to depend on the bactericidal actions of dissolved ozone exerted via its oxidizing effect. On the other hand, the effects of ozone on the periodontal tissues are largely unknown. In this paper we examined the cellular responses after OUFBW treatment. Human primary periodontal ligament fibroblasts (hPDLFs) or Ca9-22 human gingival epithelial cells were treated with OUFBW or UV-inactivated OUFBW. The production of reactive oxygen species (ROS), the activation of mitogen-activated protein kinase (MAPK) and the nuclear factor-kappa B (NF-κB) activation were analyzed. The transcript profiles of hPDLFs after OUFBW treatment were also analyzed by RNA sequencing (RNA-seq). Our results showed that OUFBW induces oxidative stress by generating ROS, which, in turn, activated the MAPK pathway. OUFBW triggered activation of c-Fos, a major component of the transcription factor activator protein 1 (AP-1), and also nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2), which possessed a high sensitivity to oxidative stress. The results of RNA-seq analysis revealed that the numerous genes involved in oxidative stress responses or MAPK signaling pathway were up-regulated after OUFBW treatment. Investigation of the signaling pathways activated by OUFBW highlights another aspect of the biological roles of OUFBW, in addition to its bactericidal activity, in the treatment of periodontitis.

Ultrafine bubble water Pengaruhnya dalam Pematahan Dormansi Benih Padi

The method for dormancy breaking could be developed by the usefull of nano bubbles technology, like ultrafine bubble water (UFB). The research aims to identify the effectivity of UFB water for dormancy breaking of rice seed. This research was arranged in a Completely Randomyzed Design with four replications and nine level treatments, i.e. control, aquadest, KNO3, UFB, and UFB 20 soaking for 24 and 48 hour, respectively. Rice seed without soaking showed the ABA content was 14.3%, aquadest, KNO3, UFB soaking for 24 hour about 10.1-11.5%, while for 48 hour about 8.9-9.9%. The maximum growth potential (MGP), more than 80% was obtained on dormancy breaking by KNO3, UFB, and UFB20 soaking for 48 hour, and UFB20 soaking for 24 hour. Aquadest soaking showed there is a increasing of MGP (less than 60%), however did not significant, as well as by 24 or 48 hour. KNO3 soaking, its dormancy breaking showed the MGP less than 80%, if the soaking less than 48 hour. The chemical scarification for dormancy breaking by KNO3, UFB, and UFB20 for 48 hour showed the radicle emergence more than 80%. The lowest radicle emergence was obtained on aquadest soaking for 24 hour, and followed by without soaking, KNO3 soaking, UFB soaking, and UFB20 soaking for 24 hour.

Characteristics of ultra-fine bubble water and its trials on enhanced methane production from waste activated sludge

In this study biogas production efficiency was evaluated by adding ultra-fine bubble water (UFBW) into waste activated sludge (WAS) through anaerobic digestion (AD). Four kinds of gases, i.e. Air, N2, CO2 and H2 were introduced into tap water (TW) to prepare the UFBW with their properties being first investigated. Results show that hundreds of millions of nanoscale bubbles with the negative zeta potential could be stable in the UFBW for longer than two weeks whereas almost no nanometer bubbles could be detected in the raw TW. As for their impact on subsequent AD of WAS, the cumulative methane production with the addition of UFBW was 14–21% higher than that from the raw TW addition group. Interestingly, the Air-UFBW also could promote the biogas production in this study, which is different from the common understanding of AD, an obligate anaerobic process.

Application of ozone ultrafine bubble water for cleaning and sterilizing the dialysis circuit

The dialysis circuit was washed by the ultrafine bubble (UFB) water and ozone UFB water and sodium hypochlorite aqueous solution. The effect of washing time, ozone concentration of UFB water, influence of UFB water on artificial dialysis equipment, etc. were investigated. The cleaning and sterilizing effect of ozone UFB water passed through the filter for artificial dialysis was verified. It was confirmed that there were no colony bacteria in the circuit which was washed by the ozone UFB water for 1 hour. Ozone UFB water has the same bactericidal effect as sodium hypochlorite. As an alternative of sodium hypochlorite, we are expecting its application to the cleaning and sterilization of ozone UFB water for dialysis circuit.

P6 ウルトラファインバブル水への超音波照射によるラジカル生成促進(ポスターセッション)

P6 ウルトラファインバブル水への超音波照射によるラジカル生成促進(ポスターセッション)

Oxygen ultra-fine bubbles water administration prevents bone loss of glucocorticoid-induced osteoporosis in mice by suppressing osteoclast differentiation.

Ultra-fine bubbles (<200nm in diameter) have several unique properties, and they are tested in various medical fields. The purpose of this study was to investigate the effects of oxygen ultra-fine bubbles (OUB) on glucocorticoid-induced osteoporosis (GIO) model mice. Prednisolone (PSL, 5mg) was subcutaneously inserted in 6-month-old male C57BL/6J mice, and 200μl of saline, OUB-diluted saline, or nitrogen ultra-fine bubbles (NUB)-diluted saline was intraperitoneally injected three times per week for 8weeks the day after operations. Mice were divided into four groups; (1) control, sham-operation+saline; (2) GIO, PSL+saline; (3) GIO+OUB, PSL+OUB saline; (4) GIO+NUB, PSL+NUB saline. The effects of OUB on osteoblasts and osteoclasts were examined by serially diluted OUB medium in vitro. Bone mass was significantly decreased in GIO [bone volume/total volume (%): control vs. GIO 12.6 vs. 7.9; p<0.01] while significantly preserved in GIO+OUB (GIO vs. GIO+OUB 7.9 vs. 12.9; p<0.05). In addition, tartrate-resistant acid phosphatase (TRAP)-positive cells in the distal femur [mean osteoclasts number/bone surface (mm-1)] was significantly increased in GIO (control vs. GIO 6.8 vs. 11.6; p<0.01) while suppressed in GIO+OUB (GIO vs. GIO+OUB 11.6 vs. 7.5; p<0.01). NUB did not affect these parameters. In vitro experiments revealed that OUB significantly inhibited osteoclastogenesis by inhibiting RANK-TRAF6-c-Fos-NFATc1 signaling, RANK-p38 MAPK signaling, and TRAP/Cathepsin K/DC-STAMP mRNA expression in a concentration-dependent manner. OUB did not affect osteoblastogenesis in vitro. OUB prevent bone loss in GIO mice by inhibiting osteoclastogenesis.

G-19 ウルトラファインバブル(UFB)水に含まれるラジカルについて

G-19 ウルトラファインバブル(UFB)水に含まれるラジカルについて

Simple surface treatment of cell-culture scaffolds with ultrafine bubble water.

We propose a novel method to treat polymeric scaffold surfaces for cell culture with water containing nanobubbles, called ultrafine bubbles (UFBs), with typical diameters less than 1 μm. A thin film of polystyrene (PS) prepared on a solid substrate was exposed to UFB water for 2 days at room temperature. The PS surface was characterized by X-ray photoelectron spectroscopy (XPS), static contact angle measurements in water, and atomic force microscopy (AFM). The surface chemical composition and wettability of PS films remained unchanged after treatment, so that aggregation states of PS at film surfaces remained unaltered by UFB water. On the other hand, after treatment, many UFBs were adsorbed on hydrophobic PS surfaces. To study the effect of UFBs on scaffold properties, the adsorption behavior of fibronectin, which is a typical extracellular matrix protein involved in cell adhesion and proliferation, was examined. While the effect on the adsorption was unclear, the structural denaturation of fibronectin was enhanced after UFB treatment, so that the proliferation of fibroblast cells on PS surfaces was promoted.