Intermittent streams are found all over the world, however most studies focus on intermittency in hot, arid climates. As flow intermittency is expected to increase with climate change, it is important to understand how stream biofilms in temperate regions respond to these changing conditions. In this study, 20 different streams from around Austria were sampled under flowing and non-flowing conditions to evaluate the effect of intermittency on temperate stream biofilms. These streams encompassed two distinct stream types: fine-grained with high agricultural land use and coarse sediments from relatively pristine areas. Half of these streams were historically intermittent and half historically perennial. Samples were taken from all streams during the spring and fall, when the intermittent streams were flowing and dry, respectively. Subsets of the sediments were subjected to controlled drying to evaluate the effects of history of intermittency on the biofilms. Samples were analyzed for respiration, extracellular enzyme activities, and extracellular polysaccharides in the wet and dry sediments from the field, as well as the lab-dried sediments. This study found that lab-dried perennial sediments showed similar responses to the intermittent sediments, indicating that history of intermittency does not affect biofilm response to drought. This study also found that the effects of grain size, seasonal growth, and nutrient levels have a larger impact on the biofilms than moisture content and history of intermittency.
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