Types Of Bioactive Compounds Research Articles

Pseudoalteromonas simplex sp. nov. Isolated from the Skin of Bandtail Puffer Fish (Sphoeroides spengleri).

A novel bacterial isolate A520T (A520T = CBAS 737T = CAIM 1944T) was obtained from the skin of bandtail puffer fish Sphoeroides spengleri (Tetraodontidae Family), collected in Arraial do Cabo (Rio de Janeiro, Brazil). A520T is Gram-stain-negative, flagellated and aerobic bacteria. Optimum growth occurs at 25-30°C in the presence of 3% NaCl. The genome sequence of the novel isolate consisted of 4.5Mb (4082 coding genes and G+C content of 41.1%). The closest phylogenetic neighbor was Pseudoalteromonas shioyasakiensis JCM 18891T (97.9% 16S rRNA sequence similarity, 94.8% Average Amino Acid Identity, 93% Average Nucleotide Identity and 51.8% similarity in Genome-to-Genome-Distance). Several in silico phenotypic features are useful to differentiate A520T from its closest phylogenetic neighbors, including trehalose, D-mannose, cellobiose, pyrrolidonyl-beta-naphthylamide, starch hydrolysis, D-xylose, lactose, tartrate utilization, sucrose, citrate, glycerol, mucate and acetate utilization, malonate, glucose oxidizer, gas from glucose, nitrite to gas, L-rhamnose, ornithine decarboxylase, lysine decarboxylase and yellow pigment. The genome of the novel species contains 3 gene clusters (~ 66.81 Kbp in total) coding for different types of bioactive compounds that could indicate ecological roles pertaining to the bandtail puffer fish host. Based on genome-based taxonomic approach, strain A520T (A520T = CBAS 737T = CAIM 1944T) is proposed as a new species, Pseudoalteromonas simplex sp. nov.

Functionalized Palygorskite as a Delivery Platforms for Bioactive Asymmetric Beta-Diketone Dyes

Natural clay minerals are among the most versatile materials used in the biomedical field. Palygorskite has found various applications in this field, from the treatment of diarrheal diseases in the past to materials with antibacterial properties and platforms carrying bioactive compounds used in the treatment of diseases, cosmetic and healthcare products in the present. In this study, a possible delivery method of some bioactive asymmetric β-diketonic compounds is presented. Palygorskite modified with amphionic groups (P) and copper ions (PCu) was used as a platform to load bioactive curcumin derivatives (1 and 2). By varying the copper ions, the amounts of charged active compounds were monitored. Studies have shown that the hybrid materials resulting from the loading of 1 and 2 compounds on palygorskite with 30% copper ions (PCu30) can be used as delivery methods for these asymmetric curcumin derivatives, while palygorskite with 50% copper ions(PCu50) loaded with the same type of bioactive compounds has antibacterial properties.

Exploring the potential of bioactive compounds in preventing cancer growth and progression: A comprehensive review

Cancer stands as a prominent global cause of mortality, with a noteworthy surge in related deaths in recent years. Over the past decade, several bioactive compounds have emerged as potent agents in impeding the relentless advance of cancer. Notably, numerous studies highlight the efficacy of natural plant-derived bioactive compounds in augmenting chemotherapy outcomes and mitigating adverse drug effects associated with chemotherapeutic agents. This comprehensive review investigates the anticancer effects of three types of bioactive compounds: polyphenols, alkaloids, and terpenoids. It elucidates their mechanisms of action and consolidates evidence from preclinical studies. Furthermore, this review delves into the molecular mechanisms through which these active compounds may manifest their anticancer properties, drawing insights from cell and animal-based studies. Keywords: Bioactive compounds, cancer prevention, polyphenols, alkaloids, terpenoids

Metabolomic Profiling Reveals the Quality Variations in Citri Reticulatae Pericarpium (Citrus reticulata Blanco cv. Chachiensis) with Different Storage Ages in Response to "Candidatus Liberibacter Asiaticus" Infection.

Citri Reticulatae Pericarpium, especially the pericarp of Citrus reticulata Blanco cv. Chachiensis (PCRC), is an important edible and medicinal ingredient for health and pharmacological properties. Citrus Huanglongbing, a devastating disease that currently threatens the citrus industry worldwide, is caused by a phloem-limited alpha-proteobacterium, "Candidatus Liberibacter asiaticus" (CLas). The industry of cultivar Chachiensis has been suffering from HLB. Although HLB affected the quality of citrus fruit, whether the quality of PCRC was affected by HLB remains unclear. In this study, we compared the metabolite profiles between HLB-affected and healthy PCRC from three sources: fresh, 6-month-old, and 9-year-old PCRC, through the untargeted LC-MS method. Compared to healthy controls, various types of bioactive compounds, mainly flavonoids, terpenoids, alkaloids, coumarins, polysaccharides, and phenolic acids, accumulated in HLB-affected PCRC, especially in the HLB-affected 9-year PCRC. In particular, isorhamnetin, isoliquiritigenin, luteolin 7-O-beta-D-glucoside, limonin, geniposide, pyrimidodiazepine, scoparone, chitobiose, m-coumaric acid, malonate, and pantothenic acid, which contributed to the pharmacological activity and health care effects of PCRC, were highly accumulated in HLB-affected 9-year-old PCRC compared to the healthy control. Multibioassay analyses revealed that HLB-affected 9-year-old PCRC had a higher content of total flavonoids and total polyphenols and exhibited similar antioxidant capacity as compared to healthy controls. The results of this study provided detailed information on the quality of HLB-affected PCRC.

Secondary Metabolite Profiling of the Endophytic Fungus Trichoderma longibrachiatum L2D2 Isolated from Anaphalis contorta

In the last few decades, research on endophytic fungi has been increasing due to its applications in agriculture, medicine, and the pharmaceutical industry. Many studies indicate that fungal endophytes produce various types of bioactive compounds. In this study, the endophytic fungus Trichoderma longibrachiatum L2D2 was isolated from the leaf of the medicinal plant Anaphalis contorta and secondary metabolites were collected. The crude extract was analyzed for the presence of seven phytochemicals, and it was observed that alkaloids, flavonoids, phenolics, steroids, tannins, and terpenoids were produced. It was also found that the endophyte extract had a total flavonoid content of 126.07 (µg of quercetin equivalent/mg) and a total phenolic content of 43.97 (µg of gallic acid equivalent/mg). The Gas Chromatography–Mass Spectrometry (GC-MS) analysis reveals the presence of the major compound Dithiooxamide, which has a retention time (RT) of 50.030%, and the Liquid Chromatography–Mass Spectrometry (LC-MS) study shows the most abundant compounds as Hexanal (RT of 3.687 min) in positive ionization and Rosamultin (RT of 24.387 min) in negative ionization. Fourier-Transform Infrared (FTIR) Spectroscopy analysis also shows the presence of alcohol, aldehyde, aliphatic ether, aliphatic primary amine, alkene, alkyl halide, amine, aromatic amine, aromatic compound, carboxylic acid, ketone, phenol, and sulfoxide as functional groups. Further studies, like the purification of bioactive compounds and their biological activities, will reveal the applicability of T. longibrachiatum L2D2 in industries.

Synthesis and Antibacterial Evaluation for Some New Schiff-bases Derived from P-aminoacetanilide

Derivatives of Schiff-bases possess a great importance in pharmaceutical chemistry. They can be used for synthesizing different types of bioactive compounds. In this paper, derivatives of new Schiff bases have been synthesized from several serial steps. The acid (I) was synthesized from the reaction of dichloroethanoic acid with 2 moles of p-aminoacetanilide. New acid (I) converted to its ester (II) via the reaction of (I) with dimethyl sulphate in the present of anhydrous of sodium carbonate and dry acetone. Acid hydrazide (III) has been synthesized by adding 80% of hydrazine hydrate to the new ester using ethanol as a solvent. The last step included the preparation of new Schiff-bases (IV-VIII) by the reaction of acid hydrazide with appropriate aromatic aldehydes and using glacial acetic acid as a catalyst. New derivatives were diagnosed by FT-IR, and by mass and 1HNMR spectroscopy (some of them). Derivatives (IV-VIII) were screened for their antibacterial against E. Coli (G-) and staph. aureus (G+). All tested compounds were found to have activity against the two kinds of bacteria.

The What and Who of Dietary Lignans in Human Health: Special Attention to Estrogen Effects and Safety Evaluation.

Lignans are a group of phenolic compounds found in plant-based diets. The human body can obtain lignans through diet, which are then metabolized into enterolignans. The enterolignans have been linked to several health benefits, including anticancer, anti-inflammatory, antioxidant effects, and estrogen effects. This review explores the relationship between the estrogenic effects of lignans and health. This review not only considers the estrogen-like activity of lignans but also discusses the safe dosage of lignans at different life stages. In addition, this review also identified other types of bioactive compounds that can act synergistically with lignans to promote health. Studies have shown that lignan administration during pregnancy and lactation reduces the risk of breast cancer in offspring. Further studies are needed to investigate the estrogenic safety effects of lignan on pregnant women and children. Whether lignans combine with other nutrients in complex food substrates to produce synergistic effects remains to be investigated. This review provides a basis for future studies on the safe dose of lignan and recommended dietary intake of lignan. We believe that the acquired as discussed here has implications for developing dietary therapies that can promote host nutrition and modulate estrogenic diseases.

Current Trends in Gelatin-Based Drug Delivery Systems.

Gelatin is a highly versatile natural polymer, which is widely used in healthcare-related sectors due to its advantageous properties, such as biocompatibility, biodegradability, low-cost, and the availability of exposed chemical groups. In the biomedical field, gelatin is used also as a biomaterial for the development of drug delivery systems (DDSs) due to its applicability to several synthesis techniques. In this review, after a brief overview of its chemical and physical properties, the focus is placed on the commonly used techniques for the development of gelatin-based micro- or nano-sized DDSs. We highlight the potential of gelatin as a carrier of many types of bioactive compounds and its ability to tune and control select drugs' release kinetics. The desolvation, nanoprecipitation, coacervation, emulsion, electrospray, and spray drying techniques are described from a methodological and mechanistic point of view, with a careful analysis of the effects of the main variable parameters on the DDSs' properties. Lastly, the outcomes of preclinical and clinical studies involving gelatin-based DDSs are thoroughly discussed.

Vibrio fluminensis sp. nov. isolated from the skin of Southern Atlantic sharpnose-pufferfish (Canthigaster figueiredoi)

This manuscript provides the description of the bacterial strain A621T characterized by Gram negative motile rods, presenting green circular colonies on TCBS. It was obtained from the skin of the sharpnose pufferfish Canthigaster figueredoi (Tetraodontidae Family), collected in Arraial do Cabo, located in the Rio de Janeiro region, Brazil. Optimum growth occurs at 20-28°C in the presence of 3% NaCl. The Genome sequence of the novel isolate consisted of 4.224Mb, 4431 coding genes and G + C content of 44.5%. Genomic taxonomy analysis based on average amino acid (AAI), genome-to-genome-distance (GGDH) and phylogenetic reconstruction placed (A621T= CBAS 741T = CAIM 1945T = CCMR 150T) into a new species of the genus Vibrio (Vibrio fluminensis sp. nov). The genome of the novel species contains four gene clusters (~ 56.17Kbp in total) coding for different types of bioactive compounds that hint to several possible ecological roles in the sharpnose pufferfish host.

대용차 개발을 위한 착색된 배(Pyrus serotina)열매로부터 항산화 화합물의 분리 동정

For alternative tea development, 2 kg of dried stained Pyrus serotina was extracted with 70 % MeOH aqueous solution, and the extract was solvent-fractionated with EtOAc, n-BuOH and Water. Two types of bioactive compounds were separated from the EtOAc fraction, by purification with silica gel and octadecyle silica gel (ODS) column chromatography. The chemical structure of the compounds were identified as quercetin (1) and geniposide (2) by analyzing spectral data with NMR, MS and IR. For the evaluation of antioxidant activity, DPPH inhibitory efficacy test and ROS production inhibitory efficacy test were performed. First, as a result of measuring the DPPH inhibitory efficacy of two samples up to the highest concentration of 50 ppm in the DPPH inhibition efficacy test, the IC50 values of NICS-1 and NICS-2 were measured to be 25.554 ppm and 22.943 ppm, respectively. In ROS production inhibitory efficacy test, the ROS production was reduced in a concentration-dependent manner in both samples, and statistically significantly inhibited ROS production compared to the UV irradiation group. According to recent reports, this study is expected to develop eco-friendly sustainable health drinks using pears and cheese and functional raw materials using pears and teeth through organic cosmetics.

Estrogenic and retinoid-like activity in stagnant waters with mass occurrence of water blooms

Stagnant freshwaters can be affected by anthropogenic pollution and eutrophication that leads to massive growth of cyanobacteria and microalgae forming complex water blooms. These can produce various types of bioactive compounds, some of which may cause embryotoxicity, teratogenicity, endocrine disruption and impair animal or human health. This study focused on potential co-occurrence of estrogenic and retinoid-like activities in diverse stagnant freshwaters affected by phytoplankton blooms with varying taxonomic composition. Samples of phytoplankton bloom biomass and its surrounding water were collected from 17 independent stagnant water bodies in the Czech Republic and Hungary. Total estrogenic equivalents (EEQ) of the most potent samples reached up to 4.9 ng·g−1 dry mass (dm) of biomass extract and 2.99 ng·L−1 in surrounding water. Retinoic acid equivalent (REQ) measured by in vitro assay reached up to 3043 ng·g−1 dm in phytoplankton biomass and 1202 ng·L−1in surrounding water. Retinoid-like and estrogenic activities at some sites exceeded their PNEC and effect-based trigger values, respectively. The observed effects were not associated with any particular species of cyanobacteria or algae dominating the water blooms nor related to phytoplankton density. We found that taxonomically diverse phytoplankton communities can produce and release retinoid-like compounds to surrounding water, while estrogenic potency is likely related to estrogens of anthropogenic origin adsorbed to phytoplankton biomass. Retinoids occurring in water blooms are ubiquitous signalling molecules, which can affect development and neurogenesis. Selected water bloom samples (both water and biomass extracts) with retinoid-like activity caused effects on neurodifferentiation in vitro corresponding to those of equivalent all-trans-retinoic acid concentrations. Co-occurrence of estrogenic and retinoid-like activities in stagnant water bodies as well as the potential of compounds produced by water blooms to interfere with neural differentiation should be considered in the assessment of risks associated with water blooms, which can comprise complex mixtures of natural and anthropogenic bioactive compounds.

Agrimonia Eupatoria L.-Incorporated Electrospun Nanofibers and Cotton Composite for Antibacterial Wound Dressing Applications

Composite wound dressings which combine the suitable properties of distinct materials into one dressing are currently being explored, in combination with different types of bioactive compounds, to enhance the healing process and avoid skin infections. In the present work, poly(vinyl alcohol) and chitosan nanofibers containing Agrimonia eupatoria L. were fabricated using a needleless electrospinning method (through Nanospider technology) and deposited on top of a 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized cotton textile dressing. The negatively charged carboxyl groups on the cotton fibers interacted with the positively charged amino groups of chitosan, which was previously blended with poly(vinyl alcohol) and Agrimonia eupatoria L. to produce the nanofiber layer. The properties of the produced composite materials were analyzed to determine the dressing’s potential for antimicrobial wound dressing applications.
 Keywords: cotton, textile dressing, electrospun nanofibers, Agrimonia eupatoria L., antibacterial composite wound dressings

Poisonous Plants of the Indian Himalaya: An Overview.

Indian Himalayan region (IHR) supports a wide diversity of plants and most of them are known for their medicinal value. Humankind has been using medicinal plants since the inception of civilization. Various types of bioactive compounds are found in plants, which are directly and indirectly beneficial for plants as well as humans. These bioactive compounds are highly useful and being used as a strong source of medicines, pharmaceuticals, agrochemicals, food additives, fragrances, and flavoring agents. Apart from this, several plant species contain some toxic compounds that affect the health of many forms of life as well as cause their death. These plants are known as poisonous plants, because of their toxicity to both humans and animals. Therefore, it is necessary to know in what quantity they should be taken so that it does not have a negative impact on health. Recent studies on poisonous plants have raised awareness among people who are at risk of plant toxicity in different parts of the world. The main aim of this review article is to explore the current knowledge about the poisonous plants of the Indian Himalayas along with the importance of these poisonous plants to treat different ailments. The findings of the present review will be helpful to different pharmaceutical industries, the scientific community and researchers around the world.

Extraction and Identification of Effective Compounds from Natural Plants

Most botanical species contain various types of bioactive compounds. This study focusses on the extraction and identification of bioactive compounds from Calicotome spinosa (Gorse), including flavones, α-linolenic acid and sugar. During the investigation of gorse flowers, leaves and bark, flavones were isolated from the bark and leaves. Calicotome spinosa showed a total isoflavonoid content of 1.5% from the bark of gorse and 1.3% from the leaves. To find the best conditions for flavone extraction, samples of Calicotome spinosa were extracted with different solvents (methanol, water and acetonitrile). Methanol was found to be a suitable solvent to selectively extract flavone. An unsaturated cis fatty acid (α-linolenic acid, C18:3 ∆9, 12, 15) was identified as the principal component of the triacylglycerol fraction from the flowers. Hydrolyses process conditions were used to study Gorse wood. The results indicated that the wood of gorse is not a suitable substance for making paper. The extracted bioactive compounds were analysed using NMR, GCMS, UV, TLC and Fibre Analyser techniques. The extracted compounds offered uses as antioxidants and agricultural chemicals in addition to other benefits.

Skrining Fitokimia dan Uji Toksisitas Ekstrak Daun Lamun Halophila Ovalis di Perairan Pantai Karma dan Pantai Serangan, Bali

Seagrass produced active compounds in a form of secondary metabolites as a survival system from predator attack and extreme environmental conditions. The active ingredient of seagrass extract can be an anticancer and antifungal. This study aimed to determine the content of active compounds and differences of toxicity level in seagrass extracts especially Halophila Ovalis in waters of Karma Beach and Serangan Beach. The method used for cytotoxicity test was Brine Shrimp Lethality Test (BSLT) using Artemia salina as an experimental animal. The result of phytochemical test showed that the extract of Halophila Ovalis leaf in Karma Beach contained bioactive compounds, such as alkaloids and tannins. Another side, the extract of Halophila Ovalis leaf in Serangan Beach contained 3 types of bioactive compounds, those were alkaloids, tannins and triterpenoids. The toxicity tests by BSLT method showed that the extract of Halophila Ovalis leaf in both research sites was non-toxic. The result was LC50 where the LC50 value of Halophila Ovalis leaf extract at the lowest to highest concentrations ie, (10, 100, and 1000 ug / ml) did not give a 50% mortality effect on Arthemi salina eggs.

Effect of solvents on bioactive compounds and antioxidant activity of Padina tetrastromatica and Gracilaria tenuistipitata seaweeds collected from Bangladesh

Seaweeds are now recognized as a treasure of bioactive compounds. However, the bioactivity of seaweed originating in Bangladesh is still unexplored. So, this study was designed to explore the secondary metabolites and antioxidant activities of solvent extracts of Padina tetrastromatica and Gracilaria tenuistipitata. Phytochemical screening and FTIR spectra confirm the diverse type of bioactive compounds. Antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power (RP), phosphomolybdenum, hydrogen peroxide and nitric oxide (NO) scavenging assays. Here, methanolic extract of P. tetrastromatica showed highest amount of total phenolic content (85.61 mg of GA/g), total flavonoid content (41.77 mg of quercetin/g), DPPH (77.07%), ABTS (77.65%), RP (53.24 mg AAE/g), phosphomolybdenum (31.58 mg AAE/g), hydrogen peroxide (67.89%) and NO (70.64%) assays compared to its methanolic extracts of G. tenuistipitata. This study concluded that methanol as a solvent extract of brown seaweed (P. tetrastromatica) exhibited bioactivity and antioxidant potentiality which will be useful for pharmacological as well as in functional food application.

Insecticidal and Antiprotozoal Properties of Lichen Secondary Metabolites on Insect Vectors and Their Transmitted Protozoal Diseases to Humans

Since the long-term application of synthetic chemicals as insecticides and the chemotherapy of protozoal diseases have had various negative effects (non-target effects, resistance), research on less harmful biological products is underway. This review is focused on lichens with potential insecticidal and antiprotozoal activity. Literature sources (27) were surveyed from five bibliographic databases and analyzed according to the taxonomic group of the insect, the protozoal disease and the lichen, the type of bioactive compounds (including method of application and mount applied), and the potential bioactivity based on mortalities caused after 24 h of exposure on insects and on parasitic protozoa. Six species of protozoa and five species of mosquitoes, three kinds of larval stages of insects and three protozoa stages were tested. Insecticidal and antiprotozoal effects of crude extracts and seven lichen secondary metabolites (mostly usnic acid) of 32 lichen species were determined. Physiological and morphological changes on parasitic protozoa were observed. Mortality rates caused by LSMs on insect vectors closer to (or somewhat above) the WHO threshold were considered to be insecticides. The results are based on laboratory experiments; however, the efficacy of metabolites should be confirmed in the field and on non-human primates to control the insect vectors and human protozoal diseases transmitted by insects.

Food Properties of Lebui Bean Powder Extract Fermented by Rhizopus Sp.

The fermentation that involves the performance of microbes is very effective for breaking the glycoside bonds that bind bioactive compounds in cells without using high temperatures and chemicals, as well as to improve the quality of nutritional components. Bioactive compounds in free form, obtained from the fermentation of black bean powder (Cajanus sp.) need to be extracted and characterized to obtain the types of bioactive compounds that have the potential to be applied in functional foods. Fermented black bean powder used in this experiment is the fermented lebui bean powder that has been fermented using the fungus Rhizopus sp. for two days. These lebui beans are local beans from Lombok Island, West Nusa Tenggara, Indonesia. This experiment aimed to determine the type and concentration of organic solvents, which can maintain the quality of nutritional contents and bioactive compounds of fermented lebui bean powder. The nested design was used. The main factor was the type of solvent (n-hexane or ethanol), while the solvent concentration (70% or 90%) was the second factor and nested in the main factor. The result showed that ethanol 90% was the best organic solvent to extract the bioactive compound in fermented lebui bean powder, and this extract contained protein, lipid, moisture, ash, fiber, and carbohydrates respectively 26.33%; 13.60%; 14.24%; 3.28%; 16.03%; and 26.54%. The bioactive compounds identified were phenolic, flavonoid, and anthocyanin, respectively 78.544 mgGAE/g; 217.358 mgQE/g; and 147.665 ppm.

Phytochemical investigation and effective therapeutic potential of plants extracts against breast and ovarian cancer cell lines: compounds from zizyphus mauritiana and triticum aestivum.

ancer is the leading cause of death, accounting for approximately one out of six people dying with this disease worldwide. Among all, the breast and ovarian cancers are top-ranked causes of women mortalities compared to other disorders. Although, there is advancement in technologies, but still, there are unresolved concerns to overcome the global disease burden. Currently, plants are being explored as a natural remedy to cure disorders. This research was planned to explore phytochemicals in methanolic extracts of Zizyphus mauritiana and Triticum aestivum, and their pharmacological activities were studied through Agrobacterium tumefaciens bacteria, in vitro breast cancer cell line and ovarian cancer cell line to find out novel candidates in disease control and prevention. Eleven different types of bioactive compounds were analysed in the tested extracts. The highest crude extracts percentage (75±0.02) was observed with Z. mauritiana. The extracts showed promising cell growth inhibition and tumor initiation inhibition in potato disc assay. MTT assay and Incucytes imaging analysis revealed that Z. mauritiana extract had a higher anticancer potential with 40 ± 0.92 cell viability against breast cancer cells (SKBR3) and 45 ±0.29 against ovarian cancer cells (SKOV3). In conclusion, these extracts could be used as chemotherapeutics owing to their cheapness, and easy availability. While detailed study is required for further purification and characterization of bioactives/target compounds and in-vivo activity confirmations.

GC-MS and FTIR analysis of methanolic leaf extract of Rhynchosia minima (L.) DC.

The current analysis was carried out to determine the chemical components in the leaves of R. minima (L.) DC. The GC-MS analysis of methanolic leaves extract of R. Minima indicated the presence of 19 compounds. The prevailing compounds of R. minima leaves were 1Pentadecene (14.31), alpha. Bisabolol (10.39%), 1Heptadecene (9.78%), Cyclohexene,4 (1,5dimethyl1,4hexadienyl (7.06%), 3Hexadecene (Z) (8.10%), Caryophyllene (6.58%), Neophytadiene (5.16%), Humulene (1.91%), Naphthalene,1,2,3,5,6,8 a-hexahydro-4,7-dimethyl (3.72%), Hexadecanoic acid, methyl ester (2.09%), Pentadecanone (3.13%), 8-Octadecanone (4.02%),1-Nonadecene (4.16%), Spiro[4.5]dec-6-en-8-one,1,7-dimethyl-4-(1-methylethyl (2.97%), Neophytadiene (2.24%),(E)-. beta.-Famesene (1.92%), Cyclohexene,4-[(1E)-1,5-dimethyl-1,4-hexadien (1.80%), Cyclohexane,octyl (1.45%), beta Bisabolene (9.21%). These compounds have antibacterial, antifungal, antioxidant, hemolytic, insecticidal, and lubricant activity. Fourier Transform Infra-Red Spectroscopy (FTIR) leaf anlysis of R. minima shows lipid, protein, phosphate ion, carboxylic acid, hydroxy compound, aliphatic bromo compounds. The present study revealed that R. minima leaves represent various types of bioactive compounds. 1-Heptadecene with antibiotic activity, 8-Octadecanone shows antimicrobial activity and hexadecanoic acid, nematicide, antibiotic, antioxidant, hypocholesterolemic production of methyl ester.