Abstract
ancer is the leading cause of death, accounting for approximately one out of six people dying with this disease worldwide. Among all, the breast and ovarian cancers are top-ranked causes of women mortalities compared to other disorders. Although, there is advancement in technologies, but still, there are unresolved concerns to overcome the global disease burden. Currently, plants are being explored as a natural remedy to cure disorders. This research was planned to explore phytochemicals in methanolic extracts of Zizyphus mauritiana and Triticum aestivum, and their pharmacological activities were studied through Agrobacterium tumefaciens bacteria, in vitro breast cancer cell line and ovarian cancer cell line to find out novel candidates in disease control and prevention. Eleven different types of bioactive compounds were analysed in the tested extracts. The highest crude extracts percentage (75±0.02) was observed with Z. mauritiana. The extracts showed promising cell growth inhibition and tumor initiation inhibition in potato disc assay. MTT assay and Incucytes imaging analysis revealed that Z. mauritiana extract had a higher anticancer potential with 40 ± 0.92 cell viability against breast cancer cells (SKBR3) and 45 ±0.29 against ovarian cancer cells (SKOV3). In conclusion, these extracts could be used as chemotherapeutics owing to their cheapness, and easy availability. While detailed study is required for further purification and characterization of bioactives/target compounds and in-vivo activity confirmations.
Highlights
The health-promoting effects of natural resources are gaining growing interest because of their role as scavengers of free radicals that cause damage in the natural human body system
This research was planned to explore phytochemicals in methanolic extracts of Zizyphus mauritiana and Triticum aestivum, and their pharmacological activities were studied through Agrobacterium tumefaciens bacteria, in vitro breast cancer cell line and ovarian cancer cell line to find out novel candidates in disease control and prevention
MTT assay and Incucytes imaging analysis revealed that Z. mauritiana extract had a higher anticancer potential with 40 ± 0.92 cell viability against breast cancer cells (SKBR3) and 45 ±0.29 against ovarian cancer cells (SKOV3)
Summary
The health-promoting effects of natural resources (plants, microbes) are gaining growing interest because of their role as scavengers of free radicals that cause damage in the natural human body system. Ovarian cancer appeared as the sixth most common cause of women's death worldwide with less than 46% overall survival rate (6,7) In such a critical scenario, natural product-based remedies with modification of previous phytoconstituents or discovery of novel bioactive compounds show promising advancements for the treatment of cancer because of their fewer side effects and highly desired biocompatibility with normal body functions (8,9). The present research was planned to explore methanolic extracts of Zizyphus mauritiana fruit part and Triticum aestivum straw part by qualitative phytochemical analysis and investigate their potential against In Vitro breast cancer cell line and ovarian cancer SKOV3 cell line. Potato disc assay was used to check the anticancer activity of listed extracts in which inhibition of tumor growth and inhibition of tumor initiation were assessed by using previously described methods (Lellau et al, 2003) (23).
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