Insertion Type Research Articles

Development of a Scaffold-on-a-Chip Platform to Evaluate Cell Infiltration and Osteogenesis on the 3D-Printed Scaffold for Bone Regeneration.

Developing a scaffold for efficient and functional bone regeneration remains challenging. To accomplish this goal, a "scaffold-on-a-chip" device was developed as a platform to aid with the evaluation process. The device mimics a microenvironment experienced by a transplanted bone scaffold. The device contains a circular space at the center for scaffold insert and microfluidic channel that encloses the space. Such a design allows for monitoring of cell behavior at the blood-scaffold interphase. MC3T3-E1 cells were cultured with three different types of scaffold inserts to test its capability as an evaluation platform. Cellular behaviors, including migration, morphology, and osteogenesis with each scaffold, were analyzed through fluorescence images of live/dead assay and immunocytochemistry. Cellular behaviors, such as migration, morphology, and osteogenesis, were evaluated. The results revealed that our platform could effectively evaluate the osteoconductivity and osteoinductivity of scaffolds with various properties. In conclusion, our proposed platform is expected to replace current in vivo animal models as a highly relevant in vitro platform and can contribute to the fundamental study of bone regeneration.

Anatomical variations of the flexor carpi ulnaris in the fetal period

Introduction: The Flexor Carpi Ulnaris (FCU) is a part of the palmar the forearm muscle group and one of the most important muscles for upper limb functioning - is responsible for flexion and adduc­tion of the hand at the radio-carpal joint. There are clinically significant but rare anatomical variations of FCU. The variability of the FCU has not been described up to now, and no typology of the muscle based on its more variable terminal attachment has been created. Aim of the study: Determination of FCU muscle typology based on available fetal material. Material and methods: A total of 114 human fetuses (53 female, 61 male) between 117 and 197 days of fetal life were eligible for the study. Preparations were carried out using classical anatomical techniques based on a previously published procedure. Thanks to that significant anthropometric landmarks were vis­ible for the gathering of metric measurements. Metric measurements were taken and statistically analysed using R-Project software. Results: A new typology was created based on variable muscle insertions. Additionally, the presence of an atypically located, additional, separated muscle belly was described. A comparison of measurements of the left upper limb in relation to the right upper limb showed significant differences for forearm length to the anthropometric point of the stylion radiale, limb length, total FCU length and FCU length which means that the left limb is longer than the right limb. A comparison of FCU insertion types between left and right upper limb showed there’s no significant difference between counts of each type. Conclusion: The FCU is a muscle that is easy to palpate and may therefore act as a topographical marker for healthcare professionals. Knowledge of its variability is not only of theoretical importance but also has clinical sig­nificance. The current publication demonstrates presence of variability in FCU terminal attachment. Certainly, this topic requires further research and continued work on a detailed understanding of forearm anatomy in the fetal period.

Complications in Laparoscopic Access in Standing Horses Using Cannula and Trocar Units Developed for Human Medicine.

First cannulation is a critical manoeuvre in equine laparoscopy. This retrospective study aimed at the comparison of the frequency and type of complications detected when using different human laparoscopy devices for laparoscopic access in standing horses, and the influence of body condition in such complications. Forty-four procedures were included, and retrieved data comprised cannula insertion technique, body condition, and type and frequency of complications. Laparoscopic access techniques were classified into five groups: P: pneumoperitoneum created using Veress needle prior to cannulation; T: sharp trocar; D: direct access via surgical incision; V: Visiport optical trocar and H: optical helical cannula (OHC). In groups T, D, V and H, access was achieved without prior induction of pneumoperitoneum. Complications were registered in 13/44 procedures, of which retroperitoneal insufflation was the most common (6/13). Statistically significant association was found between the complication incidence and the type of access, with group D showing the highest complication frequency (80%) and group H the lowest frequency (0%). The majority of complications (9/13) were observed in overweight horses. We conclude that devices designed for human patients can be used for laparoscopic access in standing horses, with the use of OHC minimizing the appearance of complications, especially in overweight horses with OW.

Unstable Total Hip Arthroplasty: Should It Be Revised Using Dual Mobility Implants? A Retrospective Analysis from the R.I.P.O. Registry.

Total hip arthroplasty (THA) is one of the most common surgical procedures in orthopedics; however, it is subjected to different kinds of failures, one of them being dislocation. Many different prosthetic designs have been developed to overcome this problem, such as dual mobility coupling. The main purpose of this article is to determine whether there are differences regarding the revision surgery of unstable THA comparing the risk of failure between dual mobility cup (DMC) implants, standard implants, and among different head sizes. A registry-based population study has been conducted by analyzing data collected by the Emilia Romagna Registry of Orthopedic Prosthetic Implants (RIPO), including a total of 253 implants failed for dislocation and instability that were operated on by cup revision surgery between 2000 and 2019. The selected population has been divided into two groups based on the insert type: standard and DMC. The age at revision surgery was significantly lower in the standard cup group with respect to DMC (p = 0.014 t-test), with an average age of 71.2 years (33-96 years range) for the standard cups and 74.8 years (48-92 years range) for the DMC group. The cumulative survival of DMC implants was 82.0% at 5-years, decreasing to 77.5% at a 10-year follow-up, which is not significantly different from standard cups (p = 0.676, Log-Rank test). DMC implants showed a significantly lower risk of re-revision for dislocation compared to standard cups (p = 0.049). Femoral heads ≥36 mm had a higher overall survival compared to smaller femoral heads (p = 0.030). This study demonstrated that DMC or femoral heads ≥36 mm are a valid choice to manage THA instability and to reduce the revision rate for dislocation at a mid-term follow-up; in those selected and targeted patients, these options should be taken into consideration because they are associated with better outcomes.

Obrada struganjem kaljenih čelika alatima od PcBN

The paper discusses the results of the experimental investigation of the performance of several grades of PcBN cutting composites in conditions of the interrupted machining of hardened steel. Both BH and BL types of PcBN indexable inserts with additions of Si3N4, TiC, and TiCN were tested, and cutting speed varied to assess the possibility of applying these cutting tools in different conditions. It has been shown that BL PcBN can be utilized for turning with shock loads in case of high cutting speed and low depth of cut (0,2 mm). While in the range of comparatively low cutting speeds BL cutting tools are actually inoperable, and because of microchipping of polycrystalline fragments and brittle destruction of the cutting edge, an increase of the cutting speed to 210 m/min changes wear pattern and formation of the wear land. As well as this, measurement of the form and values of the cutting forces impulses revealed the effect of their peak growth by up to 40%. The danger of shock impulses was analyzed by FEM modeling, and it was demonstrated that during impacts, the maximal value of the third Principal stress exceeds the average level of compressive stresses during continuous cutting by 1.6 times, which can cause damage to the cutting tool. The data on the estimation of tool fracture probability depending on loading conditions are presented, and the possibility of reducing such probability is shown.

An Overview of Best Practices for Transposable Element Identification, Classification, and Annotation in Eukaryotic Genomes.

Transposable elements (TEs) exert an increasingly diverse spectrum of influences on eukaryotic genome structure, function, and evolution. A deluge of genomic, transcriptomic, and proteomic data provides the foundation for turning essentially any non-model eukaryotic species into an emerging model to study any and all aspects of organismal biology, ultimately shaping future directions for biomedical, environmental, and biodiversity research. However, identification and annotation of the mobile genome component still lags behind the standards accepted for host gene annotation. To achieve the objective of providing every genome project with a comprehensive description of its mobilome component in addition to the standard genic and transcriptomic datasets, each step of TE identification, classification, and annotation should be focused on improving TE boundary designation, reducing identification error rates, and providing accurate information on the type and integrity of TE insertions. Here, we offer practical advice for generating TE models in de novo assemblies for non-model organisms, provide step-by-step instructions to guide inexperienced TE annotators through some of the commonly utilized TE analysis pipelines, and entertain suggestions for tool improvement which could be implemented by interested developers.

Multiple errors correction for position-limited DNA sequences with GC balance and no homopolymer for DNA-based data storage.

Deoxyribonucleic acid (DNA) is an attractive medium for long-term digital data storage due to its extremely high storage density, low maintenance cost and longevity. However, during the process of synthesis, amplification and sequencing of DNA sequences with homopolymers of large run-length, three different types of errors, namely, insertion, deletion and substitution errors frequently occur. Meanwhile, DNA sequences with large imbalances between GC and AT content exhibit high dropout rates and are prone to errors. These limitations severely hinder the widespread use of DNA-based data storage. In order to reduce and correct these errors in DNA storage, this paper proposes a novel coding schema called DNA-LC, which converts binary sequences into DNA base sequences that satisfy both the GC balance and run-length constraints. Furthermore, our coding mode is able to detect and correct multiple errors with a higher error correction capability than the other methods targeting single error correction within a single strand. The decoding algorithm has been implemented in practice. Simulation results indicate that our proposed coding scheme can offer outstanding error protection to DNA sequences. The source code is freely accessible at https://github.com/XiayangLi2301/DNA.

P065Long acting reversible contraceptive (larc) insertions in 2019 in the states with most restrictive and trigger abortion policies

<h3>Objectives</h3> As of May 2022, 26 states have been identified as certain or almost certain to ban abortion should the 1973 Roe decision recognizing women's constitutional right to choose to terminate be overturned. The purpose of this analysis was to describe the rate of insertions of long-acting reversible contraceptives (LARCs), the most effective forms of contraception, in those 26 states. <h3>Methods</h3> Data was from a HealthVerity Marketplace claims data source representing 130 million patients, representing 58% of the US census population. Women aged 15–44 were included in the analysis. For each healthcare encounter, the following data were gathered: three-digit zip code, age, implant insertion (y/n), intrauterine device (IUD) insertion (y/n), provider specialty, and payer type (Medicaid or commercial). LARC insertion rates by three-digit zip code geographic areas in the 26 states with pending abortion bans were described. <h3>Results</h3> In four states with pending abortion bans, LARC insertion rates were consistently low: <2.6% in either all or nearly all three-digit zip code areas. Four states had consistently top quartile LARC insertion rates (>4.1%). The remaining states had wide variation in LARC insertion rates; some three-digit zip code areas fell in the lowest quartile of insertions (<2.6%) and other areas showing highest percentiles of LARC insertions (>4.1%) <h3>Conclusions</h3> Use of LARCs vary among the geographic areas within the states with certain or near-certain abortion trigger bans. These variations in LARC insertion rates may indicate significant additional reproductive health burdens for women who may already be facing significant restrictions on their reproductive health choices.

The expression of Ets-1 and Fli-1 is associated with interferon-inducible genes in peripheral blood mononuclear cells from Japanese patients with systemic lupus erythematosus.

Transcription factors E26 transformation-specific-1 (Ets-1) and Friend leukemia insertion site-1 (Fli-1) and type I interferon (IFN) have been implicated in systemic lupus erythematosus (SLE). We examined the expression of these genes in peripheral blood mononuclear cells (PBMCs) from Japanese patients with SLE and analyzed their association with SLE. We enrolled 53 Japanese patients with SLE, 42 patients with rheumatoid arthritis (RA), and 30 healthy donors (HDs) (as controls) in this study. PBMCs were collected from all participants, and the expressions of Ets-1, Fli-1, and three interferon-inducible genes (IFIGs) (interferon-inducible protein with tetratricopeptide 1 [IFIT1], interferon-inducible protein 44 [IFI44], and eukaryotic translation initiation factor 2 alpha kinase 2 [EIF2AK2]) were measured using real-time polymerase chain reaction (PCR). The relationships of each molecule with clinical symptoms, laboratory data, and treatments were analyzed. The expression of Ets-1 and Fli-1 was significantly lower in the PBMCs from patients with SLE than that in the PBMCs from patients with RA and HDs. The expression of the three IFIGs was significantly higher in the PBMCs from patients with SLE than that in the PBMCs from patients with RA and HDs. For patients with SLE, significantly positive correlations were found between Ets-1 and three IFIGs; a similar trend was observed between Fli-1 and IFIGs. IFIG expression in the PBMCs was significantly higher in patients with SLE than that in other participants, and the expression of Ets-1 and Fli-1 was positively associated with IFN expression. Therefore, it was suggested that Ets-1 and Fli-1 were associated with the pathophysiology of SLE by regulating the type I IFN pathway.

Numerical investigation on thermal-hydraulic performance of air preheater equipped with helical inserts

In the present work, a numerical study has been done for the air preheater equipped with different types of helical inserts inside the tubes with segmental baffles in shell. The simulations have been carried out to analyse the heat transfer rate in terms of heat transfer coefficient and friction factor with ANSYS 18.0. The air preheater is divided into two sections shell side and tube side, which contains ambient air and flue gases respectively. The air preheater consists of 34 tubes with a length of 3 m. In the present study, three different types of inserts are investigated with a pitch to diameter ratio (p/d) of 2, 1.7 and 1.4, and the number of turns of 100, 87 and 73, respectively. The insert under study is helical shaped with circular cross-section of diameter 20 mm. Four different values of Reynolds number are used in the range of 4000–24,000. The effects of pressure drop, heat transfer coefficient on different pitch to diameter ratio (p/d) 2, 1.7 and 1.4 of inserts and on the plain tube are discussed in this present work. The results of an air preheater with inserts in tubes get higher value of heat transfer coefficient as compared to the plain tubes. The thermal performance factor of air preheater with inserts of (p/d = 1.4) shows higher factor of 2.31 as compared to the plain tubes at low Reynolds number.

Novel hybrid neuro-fuzzy model to anticipate the heat transfer in a heat exchanger equipped with a new type of self-rotating tube insert.

In this investigation, a combination of the wingsuit flying search (WFS) and teaching-learning-based optimization (TLBO) algorithms is developed as a new combinatorial optimization algorithm. The proposed combinatorial algorithm is tested over some well-known benchmark functions and then integrated with the artificial neural network (ANN) to construct a novel hybrid model. After that, the obtained hybrid model is employed to anticipate the experimentally obtained values of the average Nusselt number (Nu), average friction coefficient (f) as well as thermal-hydraulic performance ratio (η), in a heat exchanger equipped with a new type of self-rotating tube insert, against governing parameters. The insert is placed in the tube side of the water heater to heat natural gas. The proposed insert consists of various numbers of self-rotating modules. Indeed, the rotating insert is introduced to create effective secondary sweeping flow on the inner side of the tube. Since this type of tube insert simultaneously provides heat transfer enhancement and undesired pressure drop, a thermal-hydraulic performance ratio is defined to consider both of them. The governing parameters are the number of inserts (0≤N≤30), reservoir's temperature (40 °C ≤ TR≤50 °C) as well as Reynolds number (6 × 103≤Re≤18 × 103). It was found that the WFS-TLBO enhances the effectiveness of the main ANN in anticipating the Nusselt number (Nu), average friction coefficient (f) as well as performance ratio (η). Moreover, introducing the WFS-TLBO algorithm into the neural network provides an enhancement in the effectiveness of the hybrid models based on the single WFS and TLBO algorithms in anticipating the same parameters.

Zero-Strain Insertion Anode Material of Lithium-Ion Batteries.

The insertion type materials are the most important anode materials for lithium-ion batteries, but their insufficient capacity is the bottleneck of practical application. Here, LiAl5 O8 nanowires with high theoretical capacity and Li-ions diffusion coefficient are prepared and studied as an insertion anode material, which exhibits zero-strain properties upon electrochemical cycling. However, the poor electronic conductivity of LiAl5 O8 definitely sacrifices the capacity and limits the rate performance. Therefore, compact LiAl5 O8 and carbon composite are further synthesized, in which nanosized LiAl5 O8 particles are uniformly embedded in an amorphous carbon matrix. It displays a reversible capacity of 490.9 mAh g-1 at 1 A g-1 , and the capacity rises continuously to 996.8 mAh g-1 after 1000 cycles due to the interfacial storage mechanism, that the excess Li+ ions can be accommodated in the grain boundaries and C/LiAl5 O8 interfaces.

Heat Generation During Initial Osteotomy for Implant Site Preparation: An In Vitro Measurement Study

IntroductionControlling temperature generation during implant site preparation is important to prevent implant early failure.AimThe present in vitro study aimed at measuring temperature variation generated during the initial osteotomy using both rotatory and piezo-surgical inserts.MethodsNine groups were defined according to drill and insert type, cooling volume (mL/min) and cooling temperature. A total of 315 implant site preparations were performed in an artificial bone sample and the temperature was measured using an infrared camera. Drills’ wear was assessed using scanning electron microscopy at baseline and after 10 and 35 utilizations.ResultsPiezo-surgical insert groups determined a temperature increase that was significantly higher than the one generated by rotatory drills groups (p < 0.001). When considering rotatory drills groups a temperature ≥ 40 °C was never recorded.ConclusionLower saline temperature implied a significant temperature decrease (p < 0.001), while the increase in cooling volume did not imply a temperature decrease. The scanning electron microscopy analysis of the drills demonstrated that little drill wear occurred up to 35 utilization times.

Variant pectoralis minor muscle: a case study with clinical relevance.

The pectoralis minor (PMn) muscle originates from the third, fourth, and fifth ribs near the costochondral junctionusually and gets inserted on the medial margin and upper surface of the coracoid process of the scapula. To look at the morphological insertion patterns and sites of attachment of the PMn muscle in the donated cadavers. Over all 19 limbs were included in the study (9 right and 10 left). Out of 19 limbs, 10 belonged to female and 9 belonged to male cadavers. The cadavers were meticulously dissected to determine the morphological insertion types and location of the attachment of the muscle. Unusual pattern of insertion was observed in 6 limbs (31.6%) out of total 19 limbs included in the study. The variations we observed does not fall completely in the classification by Le Double, hence variations we observed can be considered as new and rare variant which to our knowledge is not reported in literature. We propose this new variant to be type 4 of Le Double classification. The potential of ectopic PMn tendon should be taken into consideration and tested out, especially in patients with shoulder discomfort and stiffening who have ruled out the more frequent diseases. For proper surgical planning, a preoperative magnetic resonance imaging or USG examination of the shoulder joint is required considering the prevalence of variation in the insertion pattern of PMn muscle. Preoperative identification of any abnormal PMn insertion can help to reduce the risk of iatrogenic tendon injury and post-operative problems.

Simple quality assurance based on filtered back projection for geometrical/irradiation accuracy in single-isocenter multiple-target stereotactic radiotherapy.

In single-isocenter multiple-target stereotactic radiotherapy (SIMT-SRT), it is difficult to evaluate both the geometrical accuracy and absorbed dose measurement when irradiating off-isocenter targets. This study aimed to develop a simple quality assurance (QA) method to evaluate off-isocenter irradiation position accuracy in SIMT-SRT and compare its feasibility with that of a commercial device. First, we created two types of inserts and metallic balls with a diameter of 5mm to be inserted into a commercially available phantom (SIMT phantom). Second, we developed a dedicated analysis software using Python for the Winston-Lutz test (WLT). Third, an image processing software, including the filtered back-projection algorithm, was developed to analyze the images obtained using an electronic portal imaging device (EPID). Fourth, the feasibility of our method was evaluated by comparing it with the results of WLT using two commercially available phantoms: WL-QA and MultiMet-WL cubes. Notably, 92% of the results in one-dimensional deviations were within 0.26mm (EPID pixel width). The correlation coefficients were 0.52, 0.92, and 0.96 in the left-right, superior-inferior, and anterior-posterior directions, respectively. In the WLT, a maximum two-dimensional deviation of 0.70mm was detected in our method, while the deviation in the other method was within 0.5mm. The advantage of our method is that it can evaluate the geometrical accuracy at any gantry angle during dynamic rotation irradiation using a filtered back-projection algorithm, even if the target is located off the isocenter. Our method can perform WLT at arbitrary positions and is suitable for the QA of dynamic rotation irradiation using an EPID.

Feasibility Analysis of Machining Cobalt-Chromium Alloy (Stellite-6) Using TiN Coated Binary Inserts.

The objective of the study was to check the feasibility of machining Stellite 6, a cobalt–chromium superalloy, using TiN-coated carbide inserts in an end milling operation. The inserts were coated using the magnetron sputtering process. The sputtering power and gas flow rate were considered as the variables during the coating process. The performance of the coated binary carbide insert was checked during the end milling of Stellite 6 (~45 HRC) through an experiment with a Taguchi design. Experimental runs based on an orthogonal array were executed for each insert type to check the feasibility of machining this cobalt-based alloy. Adequate precision and the optimum parametric conditions were determined and are reported in this study. Analysis of variance (ANOVA) with a two-factor interaction model was also undertaken to forecast the key elements influencing surface roughness. Based on the ANOVA model, the depth of the cut, combined with the insert type, was the factor that had the greatest influence on surface roughness, followed by the cutting feed, whereas the cutting velocity had the least significance based on the tests. Moreover, the regression analysis demonstrated that the created model can be used to accurately forecast surface roughness in end milling of Stellite 6 with confidence intervals of 95%.

CODE MIXING FOUND IN THE OFFICIAL TWITTER OF BKN (INDONESIA NATIONAL CIVIL SERVICE AGENCY)

This research aims to investigate the types and factors of code mixing which found in the official twitter account of BKN (Indonesia National Civil Service Agency) during the registration of civil servant’s candidate in November 2019. Qualitative approach was conducted to analyze the types and social factors of code mixing. Observation method with writing technique used to collect the data. Data reduction, data display and drawing conclusion are the process of analysis technique. To classify and explain the social factors of code mixing, Musyken’s typology of code mixing (2000) and Kim’s theory of reasons and motive of mixing code (2006) were used. From the finding, there was found that three types of code mixing such as insertion, alternation and congruent lexicalization. Insertion type of code mixing was dominated in BKN’s twitter status, followed by congruent lexicalization and the last is alternation. Most of mixing code in twitter status related with particular topic about computer technical support, online registration system, and requirements of civil servant candidates. However, there are other code-mixing factors; participant roles and relationships and internal factors (quotation).

Dynamic compressive strength of alumina ceramics

Dynamic (220–510 s−1) and quasi-static (0.001 s−1) compression experiments are conducted on alumina ceramics implemented with two types of tungsten carbide inserts, cylindrical and step-shaped. Split Hopkinson pressure bar (SHPB) tests with in-situ, high-speed optical imaging are adopted to capture the damage and failure of ceramic samples under dynamic compression. The compressive strength of alumina ceramic samples with step-shaped inserts is 15%–30% higher than that with cylindrical inserts commonly used in previous studies, under both dynamic and quasi-static loading. Damage occurs first at the two ends of ceramic samples with the cylindrical inserts, followed by edge fracture and splitting cracks penetrating the sample. However, damage is initiated in the sample region away from the sample ends for the step-shaped inserts, and oblique and secondary transverse cracks dominate the failure process. The different damage modes in the case of step-shaped inserts result in the delayed damage initiation and sample failure, and consequently high compressive strengths. Finite element modelling (FEM) of the SHPB tests provides strength and damage evolution features consistent with the experiment using the Johnson–Holmquist (JH-2) model. FEM reveals equivalent, tensile and shear stress concentrations at the two ends of samples with cylindrical inserts. The stress concentrations are responsible for the damage initiation and growth at the sample ends and the following splitting cracks, consistent with the high-speed images. In contrast, homogeneous stress distributions are achieved in the sample with the step-shaped inserts, ensuring simultaneous damage development across the sample. Overall, the step-shaped inserts in conjunction with cylindrical samples can yield reliable strength measurements for ceramics and ceramic-like materials.

Laves type intermetallic compounds as hydrogen storage materials: A review

Laves type AB 2 intermetallics belong to the most abundant group of intermetallic compounds containing over 1000 compounds. A large variety of the chemical nature of A (Mg, Ca, Ti, Zr, Rare Earth Metals) and B (V, Cr, Mn, Fe, Co, Ni, Al) metals together with the existence of the extended solid solutions formed by mixing various selected components on both A and B sites dramatically extends the list the known binary and ternary individual compounds. A vast majority of the Laves type intermetallics crystallises with C15 / FCC MgCu 2 and C14 / hexagonal MgZn 2 types of structures, both formed for a large range of ratios between the atomic radii of the A and B components outside the ideal ratio r A / r B = 1.225. Their hydrogenation performance is defined by the chemical composition and structure of the alloys and proceeds according to the following alternative / parallel mechanisms: (a) Formation of the insertion type interstitial hydrides containing up to 6–7 at. H/f.u.AB 2 ; (b) Amorphisation of the alloys on hydrogenation; (c) Disproportionation with the formation of a binary hydride of the A metal and depleted by A metal B-components based alloys/hydrides. Equilibrium pressures of hydrogen desorption from the AB 2 -type hydrides span a huge range of ten orders of magnitude and thus Laves type-based intermetallics satisfy the requirements for various applications including getters of hydrogen gas, volume- and mass-efficient hydrogen storage materials operating at ambient conditions, materials for the efficient thermally driven compression of hydrogen gas with an output pressure of several hundred bar and high capacity and high rate anode materials for the metal hydride batteries operating in a challenging temperature range - at subzero temperatures and also above 60 °C. The paper contains references to 245 publications and will guide the future work in the areas of fundamental research and also in advancing the applications of the hydrides of the Laves type intermetallics. • Laves type AB 2 intermetallics are the most abundant group of alloys. • Hydrogenation performance is defined by the chemical composition and structure. • Mechanisms of hydrogenation: interstitial hydrides / amorphization / disproportionation. • Structure-properties relationships are described. • Important applications are reviewed: H stores/MH compression/MH batteries/H getters.

Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803.

Establishing various synthetic biology tools is crucial for the development of cyanobacteria for biotechnology use, especially tools that allow for precise and markerless genome editing in a time-efficient manner. Here, we describe a riboswitch-inducible CRISPR/Cas9 system, contained on a single replicative vector, for the model cyanobacterium Synechocystis sp. PCC 6803. A theophylline-responsive riboswitch allowed tight control of Cas9 expression, which enabled reliable transformation of the CRISPR/Cas9 vector intoSynechocystis. Induction of the CRISPR/Cas9 mediated various types of genomic edits, specifically deletions and insertions of varying size. The editing efficiency varied depending on the target and intended edit; smaller edits performed better, reaching, e.g., 100% for insertion of a FLAG-tag onto rbcL. Importantly, the single-vector CRISPR/Cas9 system mediated multiplexed editing of up to three targets in parallel inSynechocystis. All single-target and several double-target mutants were also fully segregated after the first round of induction. Lastly, a vector curing system based on the nickel-inducible expression of the toxic mazF (from Escherichia coli) was added to the CRISPR/Cas9 vector. This inducible system allowed for curing of the vector in 25–75% of screened colonies, enabling edited mutants to become markerless.