Dominant Type Research Articles

Prospective Study on the Surgical Outcome of Eyelid Carcinoma

Abstract
 Introduction & Objectives : Introduction Resection of eyelid carcinoma may lead to a risk of recurrences which might increase if excision was incomplete. Risk factors of incomplete surgical removal and recurrences are deep infiltration, spreading outside the macroscopic border, eye socket penetration, and histological type of the eyelid carcinoma. Objective To evaluate the surgical outcomes of eyelid carcinomas.
 Methods : This is a hospital based prospective observational study involving 16 patients. Prior to the surgery patients were evaluated for visual acuity, intra ocular pressure, extra ocular motility, fundus examination, and local clinical examinations. The eyelid carcinomas were histological detected concerning carcinoma type and margin involvement. Local anesthesia was applied. Patients were evaluated 1 month, 3 months and 6 months after surgery for local complications, patients satisfaction on surgical results, and recurrences. The surgical technic applied in this study were also observed.
 Results : Dominant tumor type were basal cell carcinoma and squamous cell carcinoma (87, 44%). The locations were at right and left lower eyelid with percentage of 68. 7 % and 31. 3 % respectively. All patients satisfied on surgery outcomes (100%). Three patients (6. 25 %) with complications were found within 6 months after the surgery. There were no recurrences during follow up period. Surgery procedures applied were modified hughe’s procedure (56. 25%) and mass excision and reconstruction (43. 75%).
 Conclusion : There was less complications and no recurrences within 6 months after surgery. All patients satisfied with the surgical outcomes.

Higher absorption enhancement of black carbon in summer shown by 2-year measurements at the high-altitude mountain site of Pic du Midi Observatory in the French Pyrenees

Abstract. Black-carbon-containing particles strongly absorb light, causing substantial radiative heating of the atmosphere. The climate-relevant properties of black carbon (BC) are poorly constrained in high-altitude mountain regions, where many complex interactions between BC, radiation, clouds and snow have important climate implications. This study presents 2-year measurements of BC microphysical and optical properties at the Pic du Midi (PDM) research station, a high-altitude observatory located at 2877 m above sea level in the French Pyrenees. Among the long-term monitoring sites in the world, PDM is subject to limited influence from the planetary boundary layer (PBL), making it a suitable site for characterizing the BC in the free troposphere (FT). The classification of the dominant aerosol type using aerosol spectral optical properties indicates that BC is the predominant aerosol absorption component at PDM and controls the variation in single-scattering albedo (SSA) throughout the 2 years. Single-particle soot photometer (SP2) measurements of refractory BC (rBC) show a mean mass concentration (MrBC) of 35 ng m−3 and a relatively constant rBC core mass-equivalent diameter of about 180 nm, which are typical values for remote mountain sites. Combining the MrBC with in situ absorption measurements, a rBC mass absorption cross-section (MACrBC) of 9.2 ± 3.7 m2 g−1 at λ=880 nm has been obtained, which corresponds to an absorption enhancement (Eabs) of ∼2.2 compared to that of bare rBC particles with equal rBC core size distribution. A significant reduction in the ΔMrBC/ΔCO ratio when precipitation occurred along the air mass transport suggests wet removal of rBC. However we found that the wet removal process did not affect the rBC size, resulting in unchanged Eabs. We observed a large seasonal contrast in rBC properties with higher MrBC and Eabs in summer than in winter. In winter a high diurnal variability in MrBC (Eabs) with higher (lower) values in the middle of the day was linked to the injection of rBC originating from the PBL. On the contrary, in summer, MrBC showed no diurnal variation despite more frequent PBL conditions, implying that MrBC fluctuations are rather dominated by regional and long-range transport in the FT. Combining the ΔMrBC/ΔCO ratio with air mass transport analysis, we observed additional sources from biomass burning in summer leading to an increase in MrBC and Eabs. The diurnal pattern of Eabs in summer was opposite to that observed in winter with maximum values of ∼2.9 observed at midday. We suggest that this daily variation may result from a photochemical process driving the rBC mixing state rather than a change in BC emission sources. Such direct 2-year observations of BC properties provide quantitative constraints for both regional and global climate models and have the potential to close the gap between model-predicted and observed effects of BC on the regional radiation budget and climate. The results demonstrate the complex influence of BC emission sources, transport pathways, atmospheric dynamics and chemical reactivity in driving the light absorption of BC.

Consideration of within-patient diversity highlights transmission pathways and antimicrobial resistance gene variability in vancomycin-resistant Enterococcus faecium.

WGS is increasingly being applied to healthcare-associated vancomycin-resistant Enterococcus faecium (VREfm) outbreaks. Within-patient diversity could complicate transmission resolution if single colonies are sequenced from identified cases. Determine the impact of within-patient diversity on transmission resolution of VREfm. Fourteen colonies were collected from VREfm positive rectal screens, single colonies were collected from clinical samples and Illumina WGS was performed. Two isolates were selected for Oxford Nanopore sequencing and hybrid genome assembly to generate lineage-specific reference genomes. Mapping to closely related references was used to identify genetic variations and closely related genomes. A transmission network was inferred for the entire genome set using Phyloscanner. In total, 229 isolates from 11 patients were sequenced. Carriage of two or three sequence types was detected in 27% of patients. Presence of antimicrobial resistance genes and plasmids was variable within genomes from the same patient and sequence type. We identified two dominant sequence types (ST80 and ST1424), with two putative transmission clusters of two patients within ST80, and a single cluster of six patients within ST1424. We found transmission resolution was impaired using fewer than 14 colonies. Patients can carry multiple sequence types of VREfm, and even within related lineages the presence of mobile genetic elements and antimicrobial resistance genes can vary. VREfm within-patient diversity could be considered in future to aid accurate resolution of transmission networks.

Identification, Prioritization and Mapping of Major Constraints and Potentials in the Medo Watershed the Case of West Arsi District in Central Rift Valley Area of Ethiopia

The concentration to characterize the biophysical and socio-economic features in the integrated watersheds is to identify and prioritize production constraints for designing appropriate research and development interventions. The objective of this study was to assess the current biophysical of characteristics of Medo watershed, West Arsi Zone of Oromia, Ethiopia. To do so relevant data and tools were used; ArcGIS, Microsoft Excel sheet and fundamental formulae were applied for the analysis are some. The results of the study indicated that the major land use types in the watershed are agricultural land covering 50%, vegetation (shrubs, forests and, plantations) covering 24% and settlement covering 16% out of the total land use. Slope gradient of Medo watershed ranges from zero to more than 20 and the slope gradient of 2-5 and 5-10 cover the greatest in area coverage representing 201 ha and 170 ha respectively. We can also observe that about 8% out of the total area is subjected to severe erosion. Sandy clay loam was the dominant soil textural classes in the surface soils and Phaeozems, Retinols and Andosols are the dominant soil types, which cover 57%, 36% and 7% of the sub-watershed area for lower, middle, and upper slope positions, respectively. Mean annual rainfall of the area ranges from 960.09 mm at lower part of the watershed to the 1304.93 mm at upper part of the watershed. Accordingly, Medo sub-watershed is laid in majority of at sub humid agro ecological zone. An assessment of the trees within the landscape of watershed showed some remnant natural forests and a wide variety of shrubby vegetation is encountered at all landscapes. About 40 percent of the watershed, is under high to extremely severe soil loss values (>45 ton per hectare per year). The baseline study also identified that those natural resources degradation such as land, and or/ soil fertility, reduction, and recent changes in the areas’ weather condition in line of climate change prevailing in current years are few of the many factors that are contributing to the land and crop productivity reductions in the area. Therefore, prioritizing the identified problem and preparing intervention of different technologies and development plans to solve the problems by taking into consideration the existing opportunities of the watershed.

An Analysis of Speech Acts: Request in Daily Conversation

In daily conversation, people frequently communicate implicitly as an issue that is covered in illocutionary acts. Illocutionary acts have five types, they are representative/assertive, directive, commissive, expressive and declaration. This research was aimed to analyze the used of request in daily conversation in the sentences form. The request speech acts were analyzed based on the Yule theory and the type of sentences based on Frank theory. The data were taken from the daily conversation between the researcher and the speaker in daily life. The subject of this research was researcher’s friend who doing talk with the researcher. This research employed qualitative research. The result of this research showed that there are three types of sentences, namely; statement sentences, interrogative sentences, and imperative sentences. From the total data 25 sentence, the researcher found that there are 18 sentences of imperative (72%), 5 sentences of interrogative (20%), and 2 sentences of statement (2%). Therefore, the most dominant type of sentence used by the speaker is imperative sentence. Meanwhile, the least type of sentence used by speaker is statement sentence. The speaker often used imperative sentence because of its function to get the hearer to do something. Imperative sentence can be used for directive speech act because the speaker wants the hearer or target to do what the speaker says.

Lithological and Structural Factors Affecting Groundwater Occurrence in Eruwa, South West Nigeria

The subsurface Lithological and Structural framework was investigated to understand its effects on groundwater aquiferous occurrence in this study, underlined by the Precambrian Basement Complex of Nigeria, with granite, gneiss, and schist occurring as the dominant rock types. To successfully map the subsurface, fifty-two (52) Vertical Electrical Sounding points were probed to a depth of current electrode spacing of 100m depth to obtain the variation in the resistivity measurement of the underlying weathered basement and basement rock in the subsurface. Apparent resistivity values and current electrode spacing are plotted on a bi-logarithm plot and correlated to standard curves to estimate subsurface layers' depth, thickness, and resistivity values. WinResist software uses a mathematical algorithm to estimate further the geoelectric parameters to find the best-fit model that matches the observed data. Golden Surfer software was used to represent the geoelectric parameters to aid in geospatial representation. Results show 3-5 lithological layers, which include Topsoil, Clay, Laterite, Sand, Sandy Clay, and Fresh Basement with resistivity value range 82.1Ωm - 2648.2Ωm, 21.8Ωm - 98.4Ωm, 102.3Ωm -167.5Ωm, 404.6Ωm - 2468.2Ωm and 606.8Ωm - 20,535.90Ωm indicating a weathered and fresh unweathered basement rocks layers with H, KH, HA, HKH, KHKH, QH and A geophysical curve which are also typical geometry of weathered material and limited or no fractures basement rock layer. Basement rock resistivity, Overburden thickness, Reflection coefficient, longitudinal conductance, Anisotropy coefficient range (606.8Ωm-20535.9Ωm, 2.6m - 35.6m, 0.3-1.0, 0.01-0.86 and 0.7-3.3) respectively which a typical value of shallow to deep overburden layer with limited or no-detected fracture basement rock). Incorporating the geoelectric parameters value obtained and calculated reveal limited structural deformation of the basement rocks to yield fracture capable of creating secondary porosity in the area while the lithology of the weathered layers becomes the exploitable source for groundwater recovery in the study area.

Diversity of undergrowth types in frankincense stands in Humbang Hasundutan Regency

Understory plants are one of Indonesia’s biodiversity. The presence of undergrowth has an important role in the ecosystem and economy of the surrounding community. Research on the diversity of understory plants in the Frankincense forest has never been carried out. This research aims to determine the diversit y of understory plants in incense stands in Humbang Hasundutan Regency. Sampling was carried out using understory vegetation analysis plots. Analysis of the distribution pattern of incense using the standardized Morisita’s index. Based on the results of vegetation analysis at the understory level, 27 types of understory plants were found in community forests, with the most dominant type found being the fern (Diplazium esculentum) with an IVI value of 25.90%. In the protected forest, 16 types of understory plants were found with the most dominant type being Etlingera elatior with an IVI value of 31.48%. Species diversity in community forests (2.76) is in the medium category and in protected forests (2.48) is in the medium category. Species richness (R) in Community Forests is 4.34 in the medium category and in protected forests is low (2.36).

Alterations in tumor immune microenvironment and progressive immune exhaustion with advancing penile squamous cell carcinoma using multiplex immunofluorescence and image analysis.

9 Background: Penile squamous cell carcinoma (PSCC) is a rare and aggressive malignancy with limited treatment options in the advanced or recurrent settings. Immunotherapy can yield clinical responses in PSCC, but less than 20% of patients benefit. Herein, using multiplex immunofluorescence (MIF), we analyzed the composition of the tumor immune microenvironment as it varies with human papillomavirus (HPV) status and across advancing disease stages with an aim to further our understanding of how immune composition may impact clinical outcome, potential role in disease progression and response to immune therapies. Methods: Formalin-fixed and paraffin-embedded tissue samples from men with PSCC treated at Moffitt cancer center (Tampa, FL) were immunostained for CD3, CD4, CD8, CD68, PD-1, PD-L1, CD163 and CD 206 using OPAL TM 7 kit (AKOYA Biosciences) for MIF. Densities of various cell phenotypes (expressed as cells/mm2) were quantified using an automated quantitative image analysis system (InForm). Immune cell phenotype densities were stratified by HPV status and by tumor stage. We will apply the nearest neighbor cell analysis to identify the spatial orientation of immune cells in the tumor microenvironment. Results: 57 PSCC patients (median age, 60 years [IQR (interquartile range) 31-92]; 60% HPV negative) had MIF analysis. More than half (31/57) had lymph nodes involved (6 N1, 25 N2-3). The immune composition did not differ significantly with HPV status. When investigated by individual clinical stage, we observed an increase in median density of total (CD3+), helper (CD3+CD4+), cytotoxic (CD3+CD8+) and CD3+PD-L1+ T cells from N0 to N1 stage (pN0: CD3+ 44.23, CD3+CD4+ 14.77, CD3+CD8+ 16.15 cells/mm2; pN1: CD3+ 188.36, CD3+CD4+ 71.69, CD3+CD8+ 40.22 cells/mm2) followed by a decrease in N2-N3 stage (CD3+CD4+ 18.45 and CD3+CD8+ 28.22 cells/mm2).The median density of total macrophages increased with stage (pN0: 306.38; pN1: 502.47; pN2-3: 648.27 cells/mm2). While the activated M1 macrophages increased from N0 to N1 and decreased in the more advanced N2-3 stage, M2 macrophages steadily increased across stages and became the dominant type in N2-3. Conclusions: This study describes the interplay of T cells and macrophages across disease stages using MIF. We observed an initial T cell and myeloid immune response in the early locoregional stage, followed by the emergence of immune exhaustion, marked by a decline in the density of cytotoxic T cells, rising PD-L1 expression, and the progressive replacement of anti-tumor M1 macrophages with pro-tumorigenic M2 macrophages across stages. Our findings can inform treatments utilizing immune manipulation. Geospatial analysis exploring the proximity relationships of different immune cell types to each other and to the tumor is ongoing and will be presented at the meeting.

Clinical case of congenital epidermolysis bullosa in a newborn

Congenital epidermolysis bullosa (VBE) is a group of hereditary orphan genodermatoses, phenotypically and genetically heterogeneous, characterized by the formation of blisters intradermally and subdermally on the skin and mucous membranes at the site of minimal pressure or friction. The frequency of occurrence is 1:50,000 and depends on the population. VBE affects both boys and girls equally. Inheritance occurs according to the autosomal dominant and autosomal recessive type. The pathogenesis is based on mutations in genes encoding structural proteins of the epidermis, which lead to lysis of keratinocytes and disruption of epidermal–dermal connections, resulting in the formation of bullous elements and erosion. There are four main groups of VBE, depending on the location of damaged proteins and the level of destruction: simple, intermediate, dystrophic and Kindler syndrome.

Trajectories and tipping points of piñon-juniper woodlands after fire and thinning.

Piñon-juniper (PJ) woodlands are a dominant community type across the Intermountain West, comprising over a million acres and experiencing critical effects from increasing wildfire. Large PJ mortality and regeneration failure after catastrophic wildfire have elevated concerns about the long-term viability of PJ woodlands. Thinning is increasingly used to safeguard forests from fire and in an attempt to increase climate resilience. We have only a limited understanding of how fire and thinning will affect the structure and function of PJ ecosystems. Here, we examined vegetation structure, microclimate conditions, and PJ regeneration dynamics following ~20 years post-fire and thinning treatments. We found that burned areas had undergone a state shift that did not show signs of returning to their previous state. This shift was characterized by (1) distinct plant community composition dominated by grasses; (2) a lack of PJ recruitment; (3) a decrease in the sizes of interspaces in between plants; (4) lower abundance of late successional biological soil crusts; (5) lower mean and minimum daily soil moisture values; (6) lower minimum daily vapor pressure deficit; and (7) higher photosynthetically active radiation. Thinning created distinct plant communities and served as an intermediate between intact and burned communities. More intensive thinning decreased PJ recruitment and late successional biocrust cover. Our results indicate that fire has the potential to create drier and more stressful microsite conditions, and that, in the absence of active management following fire, there may be shifts to persistent ecological states dominated by grasses. Additionally, more intensive thinning had a larger impact on community structure and recruitment than less intensive thinning, suggesting that careful consideration of goals could help avoid unintended consequences. While our results indicate the vulnerability of PJ ecosystems to fire, they also highlight management actions that could be adapted to create conditions that promote PJ re-establishment.

A novel heterozygous mutation in PTHLH causing autosomal dominant brachydactyly type E complicated with short stature.

Brachydactyly type E (BDE) is a general term characterized by variable shortening of metacarpals and metatarsals, with phalanges affected frequently. It can occur as an isolated form or part of syndromes and manifest a high degree of phenotypic variability. In this study, we have identified the clinical characteristics and pathogenic causes of a four-generation pedigree with 10 members affected by BDE and short stature. After the informed consent was signed, clinical data and peripheral blood samples were collected from available family members. Karyotype analysis, array-CGH, next-generation sequencing, and Sanger sequencing were employed to identity the pathogenic candidate gene. No translocation or microdeletion/duplication was found in karyotype analysis and array-CGH; hence, a novel heterozygous mutation, c.146dupA. p.S50Vfs*22, was detected by next-generation sequencing in PTHLH gene, leading to a premature stop codon. Subsequently, the mutation was confirmed by Sanger sequencing and co-segregation analysis. In this study, we described a novel heterozygous mutation (c.146dupA. p.S50Vfs*22) of gene PTHLH in a Chinese family. The mutation could induce a premature stop codon leading to a truncation of the protein. Our study broadened the mutation spectrum of PTHLH in BDE.

Using phenology to unravel differential soil water use and productivity in a semiarid savanna

AbstractSavannas are water‐limited ecosystems characterized by two dominant plant types: trees and an understory primarily made up grass. Different phenology and root structures of these plant types complicate how savanna primary productivity responds to changes in water availability. We tested the hypothesis that productivity in savannas is controlled by the temporal and vertical distribution of soil water content (SWC) and differences in growing season length of understory and tree plant functional types. To quantify the relationship between tree, understory, and savanna‐wide phenology and productivity, we used PhenoCam and satellite observations surrounding an eddy covariance tower at a semiarid savanna site in Arizona, USA. We distinguished between SWC across two different depth intervals (shallow, <0–30 cm and deep, >30–100 cm). We found that tree greenness increased with SWC at both depths, while understory greenness was only sensitive to the shallower SWC measurements. Onset of ecosystem dormancy, estimated from satellite observations close to the eddy covariance tower, explained more variability in annual gross primary productivity (GPP) than in other phenometrics. Higher SWC led to an extended growing season, caused by delayed dormancy in trees, but the understory showed no evidence of delayed dormancy in wetter periods. We infer that the timing of ecosystem scale dormancy, driven by trees, is important in understanding changes in a savanna's GPP. These findings highlight the important effects of rainfall during the winter. These findings suggest that savanna GPP is conditional on different responses to moisture availability in each of the dominant vegetation components.

Inter-relations of precipitation, aerosols, and clouds over Andalusia, southern Spain, revealed by the Andalusian Global ObseRvatory of the Atmosphere (AGORA)

Abstract. The south-central interior of Andalusia experiences intricate precipitation patterns as a result of its semi-arid Mediterranean climate and the impact of Saharan dust and human-made pollutants. The primary aim of this study is to monitor the inter-relations between various factors, such as aerosols, clouds, and meteorological variables, and precipitation systems in Granada using ground-based remote sensing and in situ instruments including a microwave radiometer, ceilometer, cloud radar, nephelometer, and weather station. Over an 11-year period, we detected rain events using a physical retrieval method that employed microwave radiometer measurements. A composite analysis was applied to them to construct a climatology of the temporal evolution of precipitation. It was found that convective rain is the dominant precipitation type in Granada, accounting for 68 % of the rain events. The height of the cloud base is mainly distributed at an altitude of 2 to 7 km. Integrated water vapor (IWV) and integrated cloud liquid water (ILW) increase rapidly before the onset of rain. Aerosol scattering at the surface level and hence the aerosol concentration are reduced during rain, and the predominant mean size distribution of aerosol particles before, during, and after rain is almost the same. A meteorological environment favorable for virga formation is observed in Granada. The surface weather station detected rainfall later than the microwave radiometer, indicating virga according to ceilometer and cloud radar data. We used 889 rain-day events identified by weather station data to determine precipitation intensity classes and found that light rain is the main precipitation intensity class in Granada, accounting for 72 % of the rain-day events. This can be a result of the high tropospheric temperature induced by the Andalusian climate and the reduction of cloud droplet size by the high availability of aerosol particles in the urban atmosphere. This study provides evidence that aerosols, clouds, and meteorological variables have a combined impact on precipitation which can be considered for water resource management and improving rain forecasting accuracy.

Breast Tumor Classification with Enhanced Transfer Learning Features and Selection Using Chaotic Map-Based Optimization

Among women, breast cancer remains one of the most dominant cancer types. In the year 2022, around 2,87,800 new cases were diagnosed, and 43,200 women faced mortality due to this disease. Analysis and processing of mammogram images is vital for its earlier identification and thus helps in reducing mortality rates and facilitating effective treatment for women. Accordingly, several deep-learning techniques have emerged for mammogram classification. However, it is still challenging and requires promising solutions. This study proposed a newer automated computer-aided implementation for breast cancer classification. The work starts with enhancing the mammogram contrast using a haze-reduced adaptive technique followed by augmentation. Afterward, EfficientNet-B4 pre-trained architecture is trained for both original and enhanced sets of mammograms individually using static hyperparameters’ initialization. This provides an output of 1792 feature vectors for each set and then fused using a serial mid-value-based approach. The final feature vectors are then optimized using a chaotic-crow-search optimization algorithm. Finally, the obtained significant feature vectors were classified with the aid of machine learning algorithms. The evaluation is made using INbreast and CBIS-DDSM databases. The proposed framework attained a balanced computation time with a maximum classification performance of 98.459 and 96.175% accuracies on INbreast and CBIS-DDSM databases, respectively.

Effect of Roflumilast, a Selective PDE4 Inhibitor, on Bone Phenotypes in ADO2 Mice.

Autosomal Dominant Osteopetrosis type II (ADO2) is a rare bone disease of impaired osteoclastic bone resorption that usually results from heterozygous missense mutations in the chloride channel 7 (CLCN7) gene. We previously created mouse models of ADO2 (p.G213R) with one of the most common mutations (G215R) as found in humans and demonstrated that this mutation in mice phenocopies the human disease of ADO2. Previous studies have shown that roflumilast (RF), a selective phosphodiesterase 4 (PDE4) inhibitor that regulates the cAMP pathway, can increase osteoclast activity. We also observed that RF increased bone resorption in both wild-type and ADO2 heterozygous osteoclasts in vitro, suggesting it might rescue bone phenotypes in ADO2 mice. To test this hypothesis, we administered RF-treated diets (0, 20 and 100mg/kg) to 8-week-old ADO2 mice for 6months. We evaluated bone mineral density and bone micro-architecture using longitudinal in-vivo DXA and micro-CT at baseline, and 6-, 12-, 18-, and 24-week post-baseline time points. Additionally, we analyzed serum bone biomarkers (CTX, TRAP, and P1NP) at baseline, 12-, and 24-week post-baseline. Our findings revealed that RF treatment did not improve aBMD (whole body, femur, and spine) and trabecular BV/TV (distal femur) in ADO2 mice compared to the control group treated with a normal diet. Furthermore, we did not observe any significant changes in serum levels of bone biomarkers due to RF treatment in these mice. Overall, our results indicate that RF does not rescue the osteopetrotic bone phenotypes in ADO2 heterozygous mice.

Molecular Epidemiology of Escherichia coli Resistant to Carbapenems, Fluoroquinolones, and Aminoglycosides Isolated from One of the Largest Hospitals in Vietnam in 2014-2019.

Multidrug-resistant (MDR) Gram-negative bacilli including carbapenem-resistant Gram-negative Enterobacteriaceae (CRE) threaten global health. Little is known, however, about the distribution of antimicrobial resistance genes in MDR isolated from patients in Vietnamese hospitals. In this study, we collected MDR Escherichia coli, defined as E. coli resistance against all fluoroquinolones, aminoglycosides, and carbapenems. This study was designed to clarify the molecular epidemiology of Escherichia coli isolates resistant to carbapenems, fluoroquinolones, and aminoglycosides isolated from patients admitted to one of the largest hospitals in Vietnam in 2014-2019 based on both whole-genome sequencing (WGS) and phenotypic data. Methodology. Sixty-seven Vietnamese isolates screened by drug resistance by the disk test were subjected to WGS, and their sequences were analyzed to determine their multilocus sequence type (MLST), O-types, H-types, distribution of drug resistance genes, plasmid types, pathogenicity islands (PIs), virulence factor distribution, and phylogenetic evolution using the WGS data. Among the STs detected, ST410 was relatively dominant. Dominant O-types and H-types were O102 and H9 and showed some links, such as those between O102 and H8. The most dominant plasmid type and carbapenemase type were 4 and NDM-5, respectively. MLST, O-types, H-types, plasmid types, and types of carbapenemases were very heterogeneous among the isolates, with no clear correlation between them. Dominant plasmid type carrying drug resistance gene was IncQ1_1. The percentage of isolates positive for drug resistance genes, such as anti-beta-lactams and aminoglycosides, was relatively high because the isolates screened were resistant to carbapenems, fluoroquinolones, and aminoglycosides. MDR E. coli isolates isolated at a high-volume Vietnamese hospital were very heterogeneous, suggesting that they were acquired from different sources, including nosocomial infection, animals, and water. Eradication of MDR E. coli from hospitals and other clinical environments is very challenging because a single measure may be ineffective.

Hydrogen-related defects in diamond: A comparison between observed and calculated FTIR spectra

A comprehensive and up-to-date compilation of observed and calculated hydrogen (H)-related peaks that occur in the near- and middle-infrared spectra of diamond is presented. The experimental database contains >300 observed peaks attributed to H-related impurities in natural diamond. The database of calculated peaks includes data from first-principles simulations of the FTIR spectra of different VxNyHz defects in diamond and contains >300 peaks that correspond to different C-H, N-H, and B-H vibrational modes for each VxNyHz defect. Less than ~10 % of observed H-related peaks have been assigned to specific defects. Consequently, the computational database was constructed to better understand the properties of different VxNyHz defects that may correspond to different observed peaks. In general, hydrogen-rich diamonds with a dominant Type Ib character and thus poorly aggregated N, show a larger number of low-intensity, H-related peaks compared to diamonds with a dominant Type Ia character. For example, ~51 % of observed H-related peaks are only observed in Type Ib diamonds, ~9 % of peaks are only observed in diamonds with a dominant Type Ia character and ~40 % of peaks are observed in both Type Ia and Type Ib diamonds. There is a major increase in the number of distinct H-related peaks in Type IaA + Ib diamonds compared to Type Ib diamonds suggesting N-aggregation processes responsible for the formation of A-centers may also produce many distinct VxNyHz defects capable of trapping H. There is a major decrease in the number of H-related peaks in Type IaA > Ib diamonds compared to Type IaA + Ib diamonds suggesting that many of the VxNyHz defects associated with the initial production of A-centers become unstable with increasing mantle residence time and the progressive loss of C-centers. These defects likely combine (or disaggregate and then re-combine) to form fewer, relatively more intense, H-related peaks observed in Type IaA > Ib diamonds. Many of these peaks persist through continued annealing in the mantle and are observed in Type IaA, IaAB, and IaB diamonds. Our data suggests that N and H incorporation are not correlated during diamond growth and that in the early stages of diamond residence, H is incorporated into many distinct VxNyHz defects that involve C- and A-centers. With continued residence and annealing, and the progressive formation A-centers and loss of C-centers, these defects aggregate to form relatively fewer, presumably more stable, VxNyHz defects such as VN3H.

An Assessment of Ecological Sensitivity and Landscape Pattern in Abandoned Mining Land

In recent years, the development of abandoned mining land has become a focal point in landscape planning. However, during the development of abandoned mining land, there often exists a phenomenon of prioritizing economic considerations over ecological concerns, leading to a failure to achieve genuinely sustainable development. An ecological sensitivity assessment, guided by the principles of protection and development, provides an evaluation framework that directs planning strategies for abandoned mining land from the perspective of balanced development and conservation. To facilitate the development and construction of abandoned mining land, this paper utilizes GIS technology, on-site surveys, analytic hierarchy processes, etc. Taking the abandoned mining land in Haining, Zhejiang, China, as the research subject and considering its unique site conditions, ecological sensitivity is divided into topographic conditions, surface water systems, and plant landscapes. Ecological sensitivity factors are selected, and an ecological sensitivity assessment system is constructed from the perspectives of ecological conservation and sustainable development. Using ArcGIS 10.2 and Fragstats 4.2 software, landscape pattern analysis is conducted, exploring the relationship between landscape patterns and ecological sensitivity assessment results from the perspectives of landscape fragmentation, diversity, and aggregation. By comparing the results of single-factor sensitivity analysis and comprehensive sensitivity analysis, as well as landscape pattern indices before and after classifying ecologically sensitive areas, the practicality of the evaluation system is verified, facilitating planning studies and providing design recommendations for abandoned mining land. Landscape pattern indices serve as supplementary explanations for ecological sensitivity. Based on the results of ecological sensitivity assessment and landscape pattern indices, the ecological conservation levels in the research area are classified into five categories: the Level I Comprehensive Protection Zone, Level II Moderately Developed Zone, Level III Construction Suitable Zone, Level IV Core Construction Zone, and Level V Core Development Zone. These correspond to ecological protection zones, sightseeing experience zones, historical exhibition zones, core commercial zones, and themed amusement zones, respectively. The I-level sensitive area in the research area has the smallest range, while IV-level and VI-level sensitive areas have larger extents, exhibiting a high degree of overall landscape fragmentation but with diverse and dominant landscape types. Integrating ecological sensitivity assessment results and landscape pattern indices aids in delineating ecological conservation levels and regional functional recreation zones, guiding the rational recreation allocation of resources for abandoned mining land and promoting its development into a scenic area integrating ecology and tourism.

Assessing wet season water quality and health risks of Chaobai River: impacts of natural weathering and anthropogenic activities in a small urban watershed

ABSTRACT Small urban watersheds are susceptible to external influences, facing increased solute inputs during high-flow periods without sufficient dilution. This study determined the sources of major ions in the Chaobai River, which is the essential surface water source of North China. The dominant river water type was HCO3-Ca·Mg based on the Piper diagram. PCA and ion ratios revealed that natural weathering (evaporate dissolution, carbonate, and silicate weathering by carbonic and sulfuric acid) and anthropogenic activities (urban domestic sewage and industrial effluent) were the dominant factors affecting the chemical composition. Sodium adsorption ratio (SAR), soluble sodium percentage (Na%), and residual sodium carbonate (RSC) assessments indicated potential sodium and moderate salinity hazards. Meanwhile, the non-carcinogenic health risks of riverine solutes evaluated by health risk assessment need to be noticed, particularly for children. The limitation of urban sewage discharge is crucial for improving water quality in urban watershed and needs further studies.

Impact of Cleaning on Membrane Performance during Surface Water Treatment: A Hybrid Process with Biological Ion Exchange and Gravity-Driven Membranes.

In this study, the hybrid biological ion exchange (BIEX) resin and gravity-driven membrane (GDM) process was employed for the treatment of coloured and turbid river water. The primary objective was to investigate the impact of both physical and chemical cleaning methods on ceramic and polymeric membranes in terms of their stabilised flux, flux recovery after physical/chemical cleaning, and permeate quality. To address these objectives, two types of MF and UF membranes were utilised (M1 = polymeric MF, M2 = polymeric UF, M3 = ceramic UF, and M4 = lab-made ceramic MF). Throughout the extended operation, the resin functioned initially in the primary ion exchange (IEX) region (NOM displacement with pre-charged chloride) and progressed to a secondary IEX stage (NOM displacement with bicarbonate and sulphate), while membrane flux remained stable. Subsequently, physical cleaning involved air/water backwash with two different flows and pressures, and chemical cleaning utilised NaOH at concentrations of 20 and 40 mM, as well as NaOCl at concentrations of 250 and 500 mg Cl2/L. These processes were carried out to assess flux recovery and identify fouling reversibility. The results indicate an endpoint of 1728 bed volumes (BVs) for the primary IEX region, while the secondary IEX continued up to 6528 BV. At the end of the operation, DOC and UVA254 removal in the effluent of the BIEX columns were 68% and 81%, respectively, compared to influent water. This was followed by 30% and 57% DOC and UVA254 removal using M4 (ceramic MF). The stabilised flux remained approximately 3.8-5.2 LMH both before and after the cleaning process, suggesting that membrane materials do not play a pivotal role. The mean stabilised flux of polymeric membranes increased after cleaning, whereas that of the ceramics decreased. Enhanced air-water backwash flow and pressure resulted in an increased removal of hydraulic reversible fouling, which was identified as the dominant fouling type. Ceramic membranes exhibited a higher removal of reversible hydraulic fouling than polymeric membranes. Chemical cleaning had a low impact on flux recovery; therefore, we recommend solely employing physical cleaning.