Aggressive Type Research Articles

Function of Long Noncoding RNAs in Glioma Progression and Treatment Based on the Wnt/β-Catenin and PI3K/AKT Signaling Pathways.

Gliomas are a deadly primary malignant tumor of the central nervous system, with glioblastoma (GBM) representing the most aggressive type. The clinical prognosis of GBM patients remains bleak despite the availability of multiple options for therapy, which has needed us to explore new therapeutic methods to face the rapid progression, short survival, and therapy resistance of glioblastomas. As the Human Genome Project advances, long noncoding RNAs (lncRNAs) have attracted the attention of researchers and clinicians in cancer research. Numerous studies have found aberrant expression of signaling pathways in glioma cells. For example, lncRNAs not only play an integral role in the drug resistance process by regulating the Wnt/β-catenin or PI3K/Akt signaling but are also involved in a variety of malignant biological behaviors such as glioma proliferation, migration, invasion, and tumor apoptosis. Therefore, the present review systematically assesses the existing research evidence on the malignant progression and drug resistance of glioma, focusing on the critical role and potential function of lncRNAs in the Wnt/β-catenin and PI3K/Akt classical pathways to promote and encourage further research in this field.

Effect of pyridoxine or cobalamin supplementation on apoptosis and cell cycle progression in a human glioblastoma cell line

Glioblastoma is the most aggressive type of brain tumor, with current therapies failing to significantly improve patient survival. Vitamins have important effects on cellular processes that are relevant for tumor development and progression. Aim The present study explored the effect of pyridoxine or cobalamin supplementation on the viability and cell cycle progression of human glioblastoma cell line U-87 MG. Method Cell cultures were treated with increasing concentrations of pyridoxine or cobalamin for 24–72 h. After supplementation, cell viability and cell cycle progression were assessed by spectrophotometry and flow cytometry. Analysis of Bcl-2 and active caspase 3 expression in supplemented cells was performed by western blot. Result The results show that pyridoxine supplementation decreases cell viability in a dose and time dependent manner. Loss of viability in pyridoxin-supplemented cells is probably related to less cell cycle progression, higher active caspase 3 expression and apoptosis. In addition, Bcl-2 expression did not appear to be altered by vitamin supplementation, but active caspase 3 expression was significantly increased in pyridoxine-, but not cobalamin-supplemented cells, furthermore, cobalamin inhibited the pyridoxine cytotoxicity in the cell viability assay when combined Conclusion The results suggest that pyridoxine supplementation promotes apoptosis in human glioblastoma-derived cells and may be useful to enhance the effect of cytotoxic therapies in vivo.

Вплив булінгу на стан здоров’я дітей та подолання його наслідків в умовах воєнного стану (огляд літератури)

Purpose - to analyze data from the literature on the characteristics of bullying, its impact on the mental and physical health of children, and ways to overcome and prevent the consequences of this condition during the military invasion of Ukraine. For this, a search in scientometric databases was carried out, and a review of modern domestic and foreign medical literature, periodical publications, and official electronic resources was made. A review of the literature was conducted, which showed that the problems of school bullying and the consequences associated with it worsen not only mental and physical health but also have a negative impact on the quality of education of the younger generation. Bullying statistics regarding the number of completed administrative protocols in Ukrainian schools from 2019 to 2023 are given and analyzed. At the same time, human rights organizations noted that bullying was modified and became latent under martial law. It is emphasized that cases of bullying have a causal relationship with mental and physical health in adulthood. It is noted that starting in 2020, our country and the whole world will be celebrating the International Day of Combating Violence and Bullying at School. Forms of bulling, types of aggression and styles of behavior are described. It was noted that one of the important reasons for the spread of bullying is, unfortunately, its ignoring by adults. It is emphasized that children who differ in some way from others suffer most often from bullying.It is noted that under martial law, school bullying occurs more often in relation to temporarily displaced persons. It is emphasized that solving the problem of school bullying is possible through compliance with the principle of comprehensiveness between the administration of the institution, its employees, and parents. The main actors in the field of violence prevention and countermeasures who provide assistance outside educational institutions are listed. It is noted that the help and prevention of bullying consists of teaching the child to resist victimization. The role of the school nurse in the multidisciplinary medical team in overcoming the problems of school bullying and its consequences is noted. Recommendations for searching for information on organizations dealing with bullying issues on the website of the Ministry of Education and Culture of Ukraine are highlighted. It is emphasized that the most effective actions that contribute to reducing the number of cases of violence in the educational environment are systematic educational activities on non-violent behavior, implementation of high-quality preventive programs, and the promotion of anti-bullying policies. No conflict of interests was declared by the authors.

Bufotalin Suppresses Proliferation and Metastasis of Triple-Negative Breast Cancer Cells by Promoting Apoptosis and Inhibiting the STAT3/EMT Axis.

Triple-negative breast cancer (TNBC) is a highly aggressive type of breast cancer and has a poor prognosis. As standardized TNBC treatment regimens cause drug resistance and tumor recurrence, the development of new TNBC treatment strategies is urgently required. Bufotalin is a bufadienolide isolated from the skin and parotid venom glands of the toad Bufo gargarizan, and has several pharmacological properties, including antiviral, anti-inflammatory, and anticancer activities. However, the anticancer effect and underlying molecular mechanisms of action of bufotalin in TNBC have not been fully studied. In the current study, we investigated the effects of bufotalin on the growth and metastasis of MDA-MB-231 and HCC1937 TNBC cells. Bufotalin potently inhibited the proliferation of both TNBC cell lines by promoting cell cycle arrest and caspase-mediated apoptosis. Furthermore, bufotalin effectively suppressed the migration and invasion of both TNBC cell lines by regulating the expression of key epithelial-mesenchymal transition (EMT) biomarkers, matrix metalloproteinases (MMPs), and integrin α6. Notably, the anticancer effect of bufotalin in TNBC cells was associated with the downregulation of the signal transducer and activator of the transcription 3 (STAT3) signaling pathway. Collectively, our results suggest that the natural compound bufotalin may exert antiproliferative and antimetastatic activities in TNBC cells by modulating the apoptotic pathway and the STAT3/EMT axis.

Double-Expressor Lymphoma in a Young Child—A Case Report and Review of Literature

AbstractDouble hit lymphoma (DHL) and double-expressor lymphoma (DEL) are now considered as aggressive types of diffuse large B cell lymphoma. DHL is characterized by a dual rearrangement of MYC and B cell lymphoma 2 (BCL-2) and/or B cell lymphoma 6 (BCL-6) and DEL by overexpression of MYC and BCL-2. Both DHL and DEL have aggressive presentation and are more common in elderly population. We present a case of 1 ½ years old boy who presented with bilateral proptosis, and diagnosed as non-Hodgkin lymphoma with central nervous system involvement. Immunohistochemistry revealed high expression of MYC and BCL-2. Fluorescence in situ hybridization studies done to rule out DHL showed no translocation of C-MYC, Bcl-2, and Bcl-6 and hence were confirmed as double-expressor high-grade B cell lymphoma. Dual expression of C- MYC, Bcl-2, or Bcl-6 always needs further evaluation to rule out the more aggressive DHL subtypes.

Understanding Glioblastoma Signaling, Heterogeneity, Invasiveness, and Drug Delivery Barriers.

The most prevalent and aggressive type of brain cancer, namely, glioblastoma (GBM), is characterized by intra- and inter-tumor heterogeneity and strong spreading capacity, which makes treatment ineffective. A true therapeutic answer is still in its infancy despite various studies that have made significant progress toward understanding the mechanisms behind GBM recurrence and its resistance. The primary causes of GBM recurrence are attributed to the heterogeneity and diffusive nature; therefore, monitoring the tumor's heterogeneity and spreading may offer a set of therapeutic targets that could improve the clinical management of GBM and prevent tumor relapse. Additionally, the blood-brain barrier (BBB)-related poor drug delivery that prevents effective drug concentrations within the tumor is discussed. With a primary emphasis on signaling heterogeneity, tumor infiltration, and computational modeling of GBM, this review covers typical therapeutic difficulties and factors contributing to drug resistance development and discusses potential therapeutic approaches.

SURVIVAL OVER A DECADE IN A CASE OF GLIOBLASTOMA

Abstract AIMS Glioblastoma multiforme is the most aggressive type of primary brain tumours, but there is a small percentage of patients who have a long-term survival and some exceptional cases who survive decades after surgical removal of tumour along with adjuvant therapy. METHOD In 2011, a 40-year-old man, presented with headaches & confusion and underwent gliolan guided resection of a right frontal glioblastoma WHO grade IV. He received 30# of 60Gy Radiotherapy with TMZ. Chemotherapy was continued with interval scans in 2016 and 2018 showing stable disease. MRI scan in October 2022 showed contrast enhancing foci for which redo surgery was eventually performed 5 months later. The repeat histology is pending. 12 years after the primary diagnosis, the patient is clinically well. RESULTS Only a very handful cases of glioblastoma show prolonged survival of over 10 years. The long-survival for glioblastoma multiforme can be linked to age, surgical resection, molecular profile and good performance score before surgery. CONCLUSIONS These extremely rare scenarios of prolonged survival in glioblastoma serves as a motivation to continue incessant research in this field with the hope of improving life expectancy in grade 4 tumours.

Maxillary central giant cell granuloma: An unusual presentation with a difficult management

Central giant cell granuloma (CGCG) is a rare benign intraosseous aggressive lesion commonly occurring in the mandible with its 2 subtypes, non-aggressive and a relatively rare aggressive type. The present case is a rare aggressive CGCG at an unusual maxillary region associated with swelling, rapid growth, pain and cortical growth perforation. The clinical, radiographic, and histopathological features along with difficult management have been described in detail.

Anthropometric, dietary, and lifestyle factors and risk of advanced thyroid cancer: The NIH-AARP diet and health cohort study.

Most patients diagnosed with thyroid cancer have low-risk disease, but some have a higher risk for persistent or recurrent disease and even death from thyroid cancer. Few studies have evaluated potential anthropometric, lifestyle, or dietary risk factors for advanced or aggressive types of thyroid cancer. Using data from a large US cohort study, we examined associations for high-risk thyroid cancer (HRTC) and, separately, low-risk thyroid cancer (LRTC) in relation to anthropometric factors, diet, smoking, and alcohol consumption. The National Institutes of Health-American Association of Retired Persons (NIH-AARP) Diet and Health Study included 304,122 participants (124,656 women and 179,466 men) without a history of cancer who completed a mailed questionnaire in 1996-1997 and were followed for cancer incidence through 2011 via linkages with state cancer registries. Hazard ratios (HRs) for anthropometric, dietary, and lifestyle factors in relation to HRTC or LRTC, defined using guidance from the American Thyroid Association initial risk of recurrence classification, were calculated using multivariable-adjusted Cox proportional hazards regression models. During follow-up (median = 10.1 years), 426 participants were diagnosed with HRTC (n = 95) or LRTC (n = 331). In models combining men and women, baseline waist circumference (per 5 cm, HR = 1.13, 95% confidence interval [CI] 1.01-1.27) and weight gain from age 18 years to baseline age (per 5 kg, HR = 1.14, 95% CI 1.02-1.28) were positively associated with risk of HRTC but not LRTC. In contrast, vegetable intake (per cup equivalents/day, HR = 1.15, 95% CI 1.01-1.30), cigarette smoking (current vs. never, HR = 0.39, 95% CI 0.23-0.68), and alcohol consumption (per drink/day, HR = 0.83, 95% CI 0.70-0.97) were associated with risk of LRTC but not HRTC. The association of LRTC risk with vegetable intake was limited to men, and that of current smoking was more pronounced in women. Our findings suggest that greater waist circumference and adulthood weight gain are associated with thyroid cancers at higher risk for recurrence. These results may have implications for the primary prevention of advanced thyroid cancer.

P03.16.A ROLE OF BRD9 IN REGULATING RADIOTHERAPY INDUCED CHANGES IN CHROMATIN ARCHITECTURE OF GBM

Abstract Glioblastoma (GBM) is the most common, formidable, and aggressive type of adult brain tumor, with a poor prognosis. The standard treatment strategies are surgical resection, followed by radiotherapy and accompanying chemotherapy. However, 90% of patients suffer from recurrence of the disease due to the radiation resistance and chemotherapy resistance of glioblastoma cells, which exacerbate the success of the treatment, and thus, resistance remains one of the most important obstacles to overcome. The advances in molecular biology revealed that therapy resistance is related with alterations in DNA repair pathways as well as epigenetic changes governed by histone modifications and chromatin remodeling. Therefore, understanding the underlying epigenetic mechanisms that are involved in the DNA damage response has the potential to develop effective therapeutic strategies. In this manner, an epigenetic drug screen is conducted to identify novel epigenetic modifiers and their inhibitors as radiosensitizers. Bromodomain containing 9 (BRD9) was selected as target modifier while iBRD9 is selected as the radiosensitizer. Inhibition of BRD9 with a common probe, iBRD9 reduced cell viability and colony formation when combined with different doses of radiotherapy. Also, as an acetylated histone reader, inhibition of BRD9 increased the number of acetylated histones as shown by Western Blotting; where Belinostat treatment served as positive control. Currently, our efforts are directed towards understanding which genes are directly targeted by BRD9 in the presence and absence of radiotherapy. To this end, we have ectopically expressed Flag-tagged BRD9 and confirmed its expression by Western Blots. We showed that BRD9 was mainly localized to nucleus. Using this tool, we will perform ChIP sequencing and reveal radiotherapy induced changes in BRD9 binding sites.

Phytochemical and biological screening of aqueous Galium verum L. extract

Plants are an important source of active compounds with numerous therapeutic benefits. Nowadays, plant extracts have attracted an increased attention as candidates in the prevention and treatment of various diseases, including cancer. Galium verum L. is considered an effective remedy due to its extensive traditional use, thus the investigation of its anticancer potential has gained momentum. Malignant melanoma is an aggressive type of cancer with an increased incidence. Therefore, the purpose of this paper was to analyze the cytotoxic effect of GvH2O extract on A375 cells. Furthermore, the safety of the extract was evaluated on HaCaT cells and the results showed that the GvH2O does not exert a cytotoxic action on keratinocytes but stimulates their proliferation at 15 - 55 μg/mL. In addition, the GvH2O extract was characterized from a phytochemical point of view. The results showed that GvH2O contains polyphenolic compounds (rutin and chlorogenic acid), possesses antioxidant activity and can be effective on Gram-positive bacteria as well as on E. coli. GvH2O extract showed a cytotoxic effect on melanoma cells, causing changes in confluence and morphology and revealing nuclear characteristics specific to apoptosis. Finally, Galium verum L. may be a possible candidate for skin cancer therapy, but additional studies are needed.

European Integration and Countering Hybrid Threats: Challenges and Prospects

The relevance of the article’s topic is determined by the non-standard nature of the challenges and threats of today. In the modern world, hybrid threats have become a major challenge for European countries, the European Union, and the world as a whole. Hybrid warfare encompasses various forms and types of aggression that are used to influence a country’s domestic politics, public opinion, and government activities. Hybrid threats are complex and multifaceted phenomena that require a comprehensive and systemic approach to counter them. They can target different spheres of life, such as politics, economy, culture, the information space, and others. Since they have an unpredictable nature, countering them requires the development and implementation of modern strategies and effective tools at both the national and supranational levels. The European Union must develop an effective strategy to counter hybrid threats that would allow it to protect its citizens and prevent the further expansion of these threats. Therefore, the process of Eurointegration is an important element in countering hybrid threats for countries and the region as a whole. The European Union provides significant support in combating hybrid threats, including through cooperation and coordination with member countries. One important element of Eurointegration is the alignment of legislation and security standards among countries. This helps reduce risks and enhance protection against hybrid threats. Collaboration in the field of cybersecurity is also a crucial component of Eurointegration. The development and implementation of joint strategies and tools enhance the effectiveness of countering hybrid threats at the regional level. Furthermore, Eurointegration can assist countries in developing and implementing new strategies and tools to counter hybrid threats, including through financial and technical support. This will enable more effective combat against these phenomena and ensure the security of countries and the region as a whole. The EU’s approach to defining and addressing hybrid threats is outlined in the «Joint Framework on Countering Hybrid Threats - European Union Response» (2016), «Increasing Resilience and Strengthening the Capacity to Address Hybrid Threats» (2018), and the EU Security Strategy (2020). The integrated system for countering hybrid threats encompasses a wide range of measures at both the member state and supranational EU levels, including early detection, analysis, raising awareness, building resilience, prevention, crisis response, and consequence management.

Immunotherapy: a promising approach for glioma treatment.

Gliomas are the most prevalent primary malignant brain tumors worldwide, with glioblastoma (GBM) being the most common and aggressive type. Despite two decades of relentless pursuit in exploring novel therapeutic approaches for GBM, there is limited progress in improving patients' survival outcomes. Numerous obstacles impede the effective treatment of GBM, including the immunosuppressive tumor microenvironment (TME), the blood-brain barrier, and extensive heterogeneity. Despite these challenges, immunotherapies are emerging as a promising avenue that may offer new hope for the treatment of gliomas. There are four main types of immunotherapies for gliomas, immune checkpoint blockades, chimeric antigen receptor T-cell therapies, vaccines, and oncolytic viruses. In addition, gene therapy, bispecific antibody therapy, and combine therapy are also briefly introduced in this review. The significant role of TME in the process of immunotherapies has been emphasized in many studies. Although immunotherapy is a promising treatment for gliomas, enormous effort is required to overcome the existing barriers to its success. Owing to the rapid development and increasing attention paid to immunotherapies for gliomas, this article aims to review the recent advances in immunotherapies for gliomas.

Combinations of EGFR and MET inhibitors reduce proliferation and invasiveness of mucosal melanoma cells.

Mucosal melanoma (MM) is a very rare and aggressive type of cancer for which immunotherapy or targeted therapy such as BRAF/MEK inhibitors, used in cutaneous melanoma, usually fail. Due to our earlier experience showing the high effectiveness of epidermal growth factor receptor (EGFR) and hepatocyte growth factor receptor (MET) inhibitors in reducing the activation of the MAPK and PI3K/AKT signalling pathways, we aim to test whether these drugs would also be effective for mucosal melanoma. Cells representing two commercially available mucosal melanoma cell lines (GAK and HMVII) and one cell line obtained from a patient's vaginal melanoma were treated with MET or EGFR inhibitors, or combinations of these agents. The dual-inhibitor treatment strategy resulted in a decrease of cell proliferation, migration and invasion. Moreover, combinations of inhibitors led to reduction of pEGFR/EGFR and pMET/MET ratio and downregulation of PI3K/AKT and MEK/ERK1/2-based signalling pathways. Our findings indicate a potential therapeutic strategy based on EGFR and MET inhibitors in mucosal melanoma, which should be further evaluated in vivo and in clinical experiments. They also suggest that targeting multiple receptor tyrosine kinases may block signalling crosstalk and possibly delay the appearance of resistance to kinase inhibitors in mucosal melanoma cells.

Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling.

Despite the clinical benefits of androgen deprivation therapy, most patients with advanced androgen-dependent prostate cancer (ADPC) eventually relapse and progress to lethal androgen-independent prostate cancer (AIPC), also termed castration-resistant prostate cancer (CRPC). MiRNAs can be packaged into exosomes (Exos) and shuttled between cells. However, the roles and mechanisms of exosomal miRNAs involved in CRPC progression have not yet been fully elucidated. Here, we find that miR-222-3p is elevated in AIPC cells, which results in remarkable enhancement of cell proliferation, migration, and invasion ability. Furthermore, Exos released by AIPC cells can be uptaken by ADPC cells, thus acclimating ADPC cells to progressing to more aggressive cell types in vitro and in vivo through exosomal transfer of miR-222-3p. Mechanistically, Exos-miR-222-3p promoted ADPC cells transformed to AIPC-like cells, at least in part, by activating mTOR signaling through targeting MIDN. Our results show that AIPC cells secrete Exos containing miRNA cargo. These cargos can be transferred to ADPC cells through paracrine mechanisms that have a strong impact on cellular functional remodeling. The current work underscores the great therapeutic potential of targeting Exo miRNAs, either as a single agent or combined with androgen receptor pathway inhibitors for CRPC treatment.

Differences in the Clinical and Molecular Profiles of Subungual Melanoma and Acral Melanoma in Asian Patients.

Subungual melanoma (SUM) is a rare type of malignant melanoma that arises beneath the nails. SUM is categorized as a type of acral melanoma (AM), which occurs on the hands and feet. SUM is an aggressive type of cutaneous melanoma that is most common among Asian patients. Recent studies reveal that SUM and AM might have different molecular characteristics. Treatment of melanoma relies on analysis of both clinical and molecular data. Therefore, the clinical and molecular characteristics of SUM need to be established, especially during metastasis. To define the mutation profiles of SUM and compare them with those of AM, we performed next-generation sequencing of primary and metastatic tumors of SUM and AM patients. Subungual location was a better independent prognostic factor than acral location for better overall survival (p = 0.001). Patients with SUM most commonly had the triple wild-type (75%) driven by GNAQ (58%) and KIT (25%) mutations, whereas patients with AM had BRAF (28.6%) and RAF (14.3%) molecular types of mutations. Single-nucleotide variations (SNVs) were more common in SUM than in AM, whereas copy number alterations (CNAs) were more common metastatic lesions of AM. Metastatic tumors in patients with SUM and AM showed increases in CNAs (43% and 80%, respectively), but not in SNVs. The number of CNAs increased during metastasis. When compared with AM, SUM has distinct clinical and molecular characteristics.

Prognostic and clinicopathological significance of MRC2 expression in head and neck squamous cell carcinoma

BackgroundHead and neck squamous cell carcinoma (HNSCC) is one of the most aggressive types of cancers worldwide, with metastasis being the major cause of death. Recent research suggests that changes in the expression of MRC2 (mannose receptor, C-type 2) may play a role in the development and progression of various cancers; however, its expression pattern in HNSCC/ OSCC is unknown. This study aimed to elucidate the clinicopathological significance and prognostic role of MRC2 expression in HNSCC, including OSCC. Materials and methodsIn the present study, we assessed the potential roles of MRC2 in expression, prognostic value, immune infiltration and functional enrichment analysis in HNSCC patients by using different bioinformatics databases. We then validated MRC2 gene expression in 30 OSCC and adjacent normal tissue samples using quantitative reverse transcription PCR (RT-qPCR). ResultsMRC2 mRNA and protein expression were significantly upregulated in OSCC and HNSCC patients compared to that in adjacent normal tissues. Upregulated MRC2 expression was associated with poor overall survival. Increased MRC2 expression has also been linked to an aggressive clinicopathological features including advanced stages, grade, metastasis and HPV status. Interestingly, our in silico results strongly suggest that the MRC2 gene and protein interaction networks are associated with HNSCC development. Moreover, the tumor infiltration level was significantly correlated with HPV-negative HNSCC patients. ConclusionOur results suggest that MRC2 could be used as a novel prognostic marker and therapeutic target for HNSCC.

Explainable Convolutional Neural Networks for Brain Cancer Detection and Localisation.

Brain cancer is widely recognised as one of the most aggressive types of tumors. In fact, approximately 70% of patients diagnosed with this malignant cancer do not survive. In this paper, we propose a method aimed to detect and localise brain cancer, starting from the analysis of magnetic resonance images. The proposed method exploits deep learning, in particular convolutional neural networks and class activation mapping, in order to provide explainability by highlighting the areas of the medical image related to brain cancer (from the model point of view). We evaluate the proposed method with 3000 magnetic resonances using a free available dataset. The results we obtained are encouraging. We reach an accuracy ranging from 97.83% to 99.67% in brain cancer detection by exploiting four different models: VGG16, ResNet50, Alex_Net, and MobileNet, thus showing the effectiveness of the proposed method.

ТРАНСФОРМАЦИЯ МОРАЛЬНЫХ ЦЕННОСТЕЙ МОЛОДЕЖИ В ПОЛЕ ЦИФРОВОЙ КОММУНИКАЦИИ

The article is devoted to the analysis of the use of digital technologies by young people to achieve their goals, in violation of generally accepted norms of morality. The digital environment, due to its specifics of building relationships between people, can reduce the limitations of moral behavior when using digital tools. Young people often resort to one or another type of cyber aggression, which causes significant moral and psychological damage to the person it is directed at. It is obvious that teenagers and young people do not always know how to resort to self-regulation in the digital space, which differs significantly in stereotypes of building relationships from real life. Digital culture as a task of digital socialization includes the education of behavioral stereotypes based on special social responsibility in the digital environment. As a research methodology, the concepts of social education and social development of a person in a digital society, as well as the concepts of life strategies of a person focused on basic values - deontological, consequentialist, contractarian and existentialist - were used in the work...

Cannabis sativa a Potential Anticancer Treatment in Melanoma Cancer Cells

Introduction Melanoma cancer is the most aggressive skin cancer type with a poor prognosis. Chemotherapy has been used in the past in treating melanoma cancer, however, the method usually results in toxicity. Therefore, a call has been made to develop cancer treatments using medicinal plants to reduce drug toxicity. The aim of this study was to establish the role of RBBP6 in melanoma cancer development and progression. The hypothesis of this study was that RBBBP6 is highly expressed in melanoma cancer and serves as an early biomarker. Results obtained supported the hypothesis since high expression of RBBP6 in melanoma cancer was observed in untreated control melanoma cells. Methodology 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Cytosmart fluorescence, and quantitative Polymerase Chain Reaction (qPCR) were used. Results The IC50 values of Cannabis sativa EtOH and MeOH extracts were obtained at 51.31315 and 57.34135 µg/mL, respectively, for A375 cells. Normal cells were used to check if there are any serious cytotoxic effects, IC50 values for Cannabis sativa EtOH and MeOH extracts were obtained at 79.577850 and 59.5754 µg/mL, respectively. RMG-1 cells showed IC50 at 84.748 and 34.66475 µg/mL for Cannabis sativa EtOH and MeOH extracts, respectively. CYTOSMART LUX3 bright field microscope was used for morphological analysis of both A375 and RMG-1 cells and changes were observed after treating with Cannabis sativa EtOH and MeOH extracts. Apoptosis analysis to determine the mode of cell death was done using a CYTOSMART fluorescence microscope. Early apoptotic effects were observed in both Cannabis sativa EtOH and MeOH extracts on A375 cells. Early apoptotic effects were also observed after treating RMG-1 cells with EtOH extract. Caspase 3/7 activity was done on both A375 and RMG-1 cells, and caspase 3/7 activity were increased after treating with Cannabis sativa EtOH and MeOH extracts. Real Time Polymerase Chain Reaction (RT-PCR) showed that RBBP6 and BCl2 were down-regulated after treating with Cannabis sativa EtOH and MeOH extracts. Conclusion siRNA co-treatment with EtOH and MeOH extracts resulted in low expression of both RBBP6 and BCl2. P53 was upregulated after treatment with the extracts and co-treatment with the extracts and siRNA.