Type Locality Research Articles

Rediscovery of Portulaca trianthemoides (Portulacaceae) in southern Africa after 89 years

Portulaca trianthemoides was described in 1931 and known previously only from the type locality in Soutpansberg, Limpopo, South Africa. It has not been collected since 1934; however, a recent rediscovery of the species led us to investigate the name (typification) and propose an emended description. Some aspects of the ecology of the species are also discussed, and first images of live plants are provided. Despite its restricted distribution area, an estimation of the population size is more than c. 15,000 individuals, suggesting that the species is not of conservation concern.

Redescription of Proteocephalus fallax La Rue, 1911 (Cestoda) and a list of proteocephalid tapeworms of whitefish (Coregonus spp.).

Tapeworms of the genus Proteocephalus Weinland, 1858 (Cestoda: Proteocephalidae) are common and widespread intestinal parasites of whitefish (Coregonus spp., Salmonidae: Coregoninae). Previous taxonomic studies, based solely on morphology and inconsistently fixed specimens, concluded that all salmoniform fish, including whitefish, are parasitised by a single euryxenous and highly polymorphic species, Proteocephalus longicollis (Zeder, 1800). However, recent molecular phylogenetic analyses have revealed the existence of several species specific to individual genera or even species of salmoniform fish. In this study, Proteocephalus fallax La Rue, 1911 is redescribed based on newly collected and genetically characterised specimens from several Coregonus species in Switzerland, the type locality of the species, and in Norway. This cestode was previously synonymised with P. exiguus La Rue, 1911, a parasite of whitefish in North America, but the two species are not closely related. Proteocephalus fallax differs from P. exiguus in its larger body size, wider proglottids, shorter cirrus sac and broader scolex. In addition, the other Proteocephalus species described in whitefish are briefly discussed, with comments on their validity, host range and distribution.

Evidence for the proposal that Palisada corallopsis and P. furcata (Ceramiales, Rhodophyta) are conspecific

Abstract Palisada corallopsis has a widely reported distribution in the western and eastern Atlantic and some areas of the Indo-Pacific Ocean, whereas P. furcata has a more restricted distribution, reported only from Brazil and Cuba. Palisada corallopsis and P. furcata were collected from their type localities, Havana, Cuba and Ceará, Brazil, respectively, and compared by morphological and molecular analyses using rbcL and COI-5P genes. The molecular results placed the sequences from the type locality of both species in the same clade, showing low genetic divergence, 0.95 % for rbcL and 1.6 % for COI-5P. Morphologically, the species share similar habits, with branchlets bi-to trifurcated, cortical cells that may be arranged as a palisade and connected by secondary pit-connections. Our detailed morphological observations, including examination of the type specimens, and molecular analyses demonstrate that P. corallopsis and P. furcata are conspecific, P. furcata being a later taxonomic synonym of P. corallopsis.

A new species of Pitcairnia (Bromeliaceae) with nocturnal anthesis from Oaxaca, Mexico

Background: Pitcairnia is the third largest bromeliad genus in Mexico with 57 species and ca. 82.5 % endemism, and is characterized mostly by zygomorphic, ornithophilous flowers of diurnal anthesis in several hues of red, orange, or yellow, whereas white or green flowers are uncommon. Moreover, there are just a few species with nocturnal anthesis in Megamexico. One of them is documented and discussed here. Questions: Is the Pitcairnia with campanulate, fragrant flowers of nocturnal anthesis from Oaxaca different from other species with similar characters native to Mexico and the Neotropics and what is its conservation status? Studied species: Pitcairnia abundans, P. lokischmidtiae, P. zapoteca. Study site and dates: Oaxaca, Mexico, 2016-2024. Methods: Live plants, herbarium material, protologues, and high-resolution images of herbarium specimens, both of the new taxon and of morphologically similar taxa, were studied and compared against each other. The conservation status of the new species was assessed using the IUCN criteria. Results: Pitcairnia zapoteca is similar but different at the species level from P. lokischmidtiae from Jalisco; both share campanulate flowers of nocturnal anthesis and a musky or frutal fragances. The conservation status of the new taxon is assessed as DD. Conclusions: Pitcairnia zapoteca is a new species, only known from the type locality in the state of Oaxaca and only one of three Mexican species with campanulate, white, yellowish or greenish flowers of nocturnal anthesis. The assessment the true conservation status of the new taxon requires detailed exploration of the SE portion of Oaxaca.

A new species of the pill millipede genus Rhopalomeris Verhoeff, 1906 (Diplopoda, Glomerida, Glomeridae) from Myanmar, and notes on Rhopalomeriscarnifex (Pocock, 1889).

The taxonomy of the pill millipede genus Rhopalomeris Verhoeff, 1906, which is restricted to Indochina and currently comprises six described species, is refined and updated. An integrative taxonomic approach was employed that combines morphological examination with DNA barcoding using the cytochrome c oxidase subunit I (COI) gene for species identification and delineation. The first objective was to confirm the identity of Rhopalomeriscarnifex (Pocock, 1889), a charismatic species known as the "candy pill millipede" due to its vivid coloration, based on specimens collected near the type locality in Myanmar. The second objective was to describe a new species, Rhopalomerisnigroflava Likhitrakarn, sp. nov., discovered in Linno Gu, Kayin State, Myanmar. This new species is distinguished by its small body size (5.1-9.7 mm long) and yellow body with contrasting brown to blackish markings on certain terga. In addition, the position of the telopod syncoxital lobe relative to the lateral syncoxite horns separates it from other Rhopalomeris species. The interspecific divergence between R.nigroflava Likhitrakarn, sp. nov. and other congeners ranges from 10.85% to 16.13%, based on uncorrected COI p-distances, while the intraspecific divergence was 0%-7.44%. A distribution map of and a revised identification key to all known species of Rhopalomeris are also provided.

Taxonomic Data Objects for Communicating the Meaning of Species Names

A central problem in biodiversity data science remains the inability to precisely aggregate observations that originate from the same species. Current approaches still rely heavily on the ‘Genus species’ pair of Linnaean taxonomy to label and group data. However, such binomial species names do not alone contain enough information to distinguish variation in a name’s conceptual usage (i.e., meaning) across time, place, or investigator. This “species name-to-meaning” problem is well known, but no robust and scalable solutions yet exist (Berendsohn 1995Bisby 2000, Sutherland et al. 2000, Page 2007, Franz and Sterner 2018, Upham et al. 2021, Sterner et al. 2023, Sterner et al. 2020, Beach et al. 1993). The problem is widespread especially for well-studied groups like mammals, for which 45% more species are now recognized than 30 years ago, including many species splits and rearrangements (Burgin et al. 2018, Mammal Diversity Database 2024). To address this problem, we here propose to digitally package and sign the relevant taxonomic data associated with a given species name usage to form a ‘Taxonomic Data Object’ (TDO). These TDOs are conceptually similar to 'secundus' references (i.e., species name sec. author, Berendsohn 1995) but differ in being machine readable and thus operational from the perspective of high-throughput data science. TDOs aim to move from analog secundus references, which require a human to track meaning by reading the cited article, to a digital and machine-actionable taxonomic reference. Versioned TDOs that are digitally signed using hash algorithms (e.g., md5 or sha256, see Elliott et al. 2023) will allow for precisely communicating, with verified data provenance from sender to receiver, certain aspects of what a species name means according to a given authority at a given time. Example TDO contents include DarwinCore terms (e.g., scientificName, namePublishedIn, nameAccordingTo, nomenclaturalStatus (Darwin Core Maintenance Group 2021)) as well as meaning-rich digital assets like geographic range maps (e.g., GeoJSON format), exemplar DNA sequences (e.g., FASTA format or National Center for Biotechnology Information (NCBI) accession number), holotype specimen information (e.g., catalog number, type locality coordinates), and taxonomic treatment texts (including material citations, e.g., as digitized and extracted by Plazi TreatmentBank, Agosti and Egloff 2009; see Fig. 1). We discuss pilot examples comparing global bat species according to the v1.2 vs. v1.11 taxonomies of the Mammal Diversity Database (Sep 2020 vs. Apr 2023), showing how TDOs enable the reliable tracking of taxonomic meaning for the 63 bat species affected by splits during this period. We posit that widespread use of TDOs will enable the traceable exchange of taxonomic information across a variety of existing platforms, providing a path for accurate species-level data aggregation at global scales, at least for well-studied taxa.

An overview of the Brazilian Atlantic Forest species of Pachythone Bates (Lepidoptera: Riodinidae), with the description of a new species and an updated checklist of Pachythonina

The taxonomy of the Brazilian Atlantic Forest endemic species belonging to the Neotropical metalmark genus Pachythone Bates, 1868 is revised. A new endemic species is described, Pachythone gagarini Dolibaina & Dias, sp. nov., based on historical specimens from a single locality in Rio de Janeiro state. Adults and genitalia of the new species are illustrated and described in detail. Pachythone palades Hewitson, (1873) and P. pasicles Hewitson, (1873), described from females and males, respectively, and regarded as two distinct species since their descriptions, are here recognized as a single species, the former being considered a junior subjective synonym of the latter (syn. nov.). Lectotypes are designated for both P. palades and P. pasicles. Pachythone sumare Callaghan, 1999, so far known from its type locality in Rio de Janeiro state, is here reported from Joinville, Santa Catarina, which extends its occurrence 700 km southwards. Lastly, an updated checklist for the recently proposed subtribe Pachythonina is given.

Leptometa cynthia – a new species from the Democratic Republic of the Congo (Lepidoptera, Lasiocampidae, Lasiocampinae, Argudini)

A new species of the genus Leptometa Aurivillius, 1927, Leptometa cynthia sp. n., is described from the rich Congolian forests. The new species is compared with the morphologically closest and sympatric Leptometa sapelensis Aurivillius, 1927 (type locality “Nigeria: Sapele”). Adult males and their genitalia are illustrated.

Community characteristics and population dynamics of Osmanthus pubipedicellatus, a rediscovered critically endangered species based on morphological and molecular evidence

AbstractConservation of plant diversity is essential in the current status of rapid biodiversity loss. A critically endangered species of Oleaceae, Osmanthus pubipedicellatus was rediscovered in its type locality after nearly a century based on morphological and molecular evidence, further supplemented by a revised description. Meanwhile, the static life table was established, the survival curve was drawn, and the quantitative analysis of population dynamics was made to evaluate the populations status of O. pubipedicellatus. Additionally, we analyzed the community characteristics to understand its natural habitat. The results showed that O. pubipedicellatus mainly grows in dense forests dominated by plants from Lauraceae and Fagaceae. The static life table, survival curve, and quantitative analysis of population dynamics showed that the population is fluctuating and vulnerable to external disturbance. In general, the population of O. pubipedicellatus was characterized by few individuals, lacking of seedlings and small trees, a single peak pattern of age class distribution and a trend to decline in the future. Consequently, we strongly recommend that more field work and conservation measures should be taken to suppress the decline of the O. pubipedicellatus population.

A new genus and a new species of Sphodrini (Coleoptera: Carabidae) from Changbai Mountain, China, with notes on the tribe

A new genus and a new species of the sphodrine subtribe Dolichina are described: Peiyuia changbaiensis Zhu & Shi, gen. nov., sp. nov. (type locality: China, Jilin, Changbai Mountain, 42.1077°N, 128.0957°E, altitude 1415 m). A key is provided for the eight known genera of Dolichina. Relationships of the genera of Dolichina and some characters of the subtribe are discussed. Two species of Sphodrini are newly reported from China: Morphodactyla coreica (Jedlička, 1937) and Pristosia latistoma Sasakawa, J.L. Kim, J.K. Kim & Kubota, 2006.

Abrolophus from Australasia, and the mysterious Mypongia (Trombidiformes: Parasitengona: Erythraeidae)

An undetermined species of Abrolophus Berlese was intercepted in quarantine by Australian biosecurity on kiwifruit imported from New Zealand. Here, we show that these are the Australian species Abrolophus ripicola (Womersley, 1934), a species known from the post-larval stages only. We present evidence that the larva of A. ripicola is probably the taxon Mypongia brevipes Southcott, 1961, which belongs to a monotypic genus with unique morphology that is placed in a different subfamily to Abrolophus. The larval and post-larval stages we examined share a similar morphology, most notably a reduction of the prodorsal shield and much reduced leg setation. Three M. brevipes larvae were intercepted on kiwifruit imported from New Zealand, and with an additional larval specimen from South Australia, these four specimens represent the first collections of this taxon since its description. Further circumstantial evidence supporting the conspecificity of the two taxa includes a female specimen of A. ripicola collected from Myponga, the type locality of M. brevipes, and larval stages of an undescribed Mypongia collected together with the post-larval stages of an undescribed Abrolophus, from the same locality in South Africa. Keys are provided for the post-larval stages of Abrolophus species in Australia (6 spp.), Papua New Guinea (Abrolophus novaeguinensis (Gunther, 1941)) and New Zealand (Abrolophus zelandicus Luxton, 1989).

Review of Oriental species of the genus Atopotrophos (Hymenoptera: Ichneumonidae)

A new species of tryphonine Darwin Wasps in the genus Atopotrophos Cushman, 1919, (A. bubeng sp. nov.). is described from Bubeng, Mueang La, Xishuangbanna, Yunnan Province. The new species can be distinguished from all other Atopotrophos species by having 35 flagellomeres, a very short malar space, both scutellum and postscutellum with apical teeth, and from the most similar species, A. spiniger, by its larger size, longer area superomedia, longer T1, and shorter ovipositor. Re-descriptions, illustrations of holotype specimens of A. chinensis and A. spiniger are provided. The type localities of these species are defined, and approximate coordinates and habitat description are added. A key for Oriental species is provided.

Two new species of the genus Quindina Roewer, 1914 (Opiliones: Nomoclastidae) from Colombia: phylogenetic relationships and notes on their nest architecture

ABSTRACT Quindina Roewer, 1914 is a nomoclastid genus with 11 described species behaviourally characterised by its remarkable nest-building behaviour. In this paper we describe two new Colombian species of the genus from the western range of the Northern Andes, based on genital morphology and external characters. In addition, we explore their phylogenetic relationships using a phenotypic matrix with 96 characters, three newly proposed, and 55 terminals including all described species of Quindina and a wide outgroup sample across the suborder Laniatores. Quindina sanantonio sp. n. (type locality: Valle del Cauca, Santiago de Cali, San Antonio Forest) can be diagnosed by having two olive-green patches behind the ocularium and on the lateral portion of areas II and III, connected on the posterior margin of area III. Quindina horologium sp. n. (type locality: Risaralda, Santuario, Planes de San Rafael) can be diagnosed by having an hourglass-shaped dorsal pattern, beginning as two emerald-green patches next to the ocularium and continuing to the posterior margin of area III. Both species build nests without walls on the underside of leaves (ie flattened nest type), a previously unknown nest architecture for the genus. The phylogenetic analysis confirmed the monophyly of Quindina. The two new species, herein described, were recovered as basally branching within the genus. The inferred ancestral state for the nest architecture was the flattened nest type. We provide an updated key for the Colombian species of the genus Quindina and an updated distribution map. http://www.zoobank.org/urn:lsid:zoobank.org:pub:4C811186-3FAC-4F65-81BD-A5720513551E.

A A new species of Graffenrieda (Melastomataceae) from the northern Andes, Caquetá, Colombia, and a key for the Graffenrieda group with calyptrate flowers

Background and Aims: Graffenrieda is a Neotropical genus with more than 70 species, mainly distributed in the Guiana Shield and the Andes. This work describes a new species of Graffenrieda and provides a key to identify all species with calyptrate flowers in the genus.Methods: The description of this new species was made from collections deposited in the Colombian herbaria JBB and COAH. Measurements of vegetative parts and inflorescence were made from dried material, while floral structures and fruits were measured from fresh material preserved in alcohol. Their conservation status was assessed according to IUCN guidelines. A key for the recognition of all species of the genus with calyptrate flowers, including the new species and Centronia species to be transferred to Graffenrieda is also provided here, for which lists of species in Neotropical countries, collection databases, and literature on both genera were consulted.Key results: Graffenrieda cardenasii sp. nov. is endemic to the southern Cordillera Oriental of Colombia and is characterized by its indumentum of irregular lepidote trichomes on the vegetative parts, leaves with incomplete lateral anisophylly, leafy bracteoles as large as the flower buds, 5-merous flowers, calyptrate calyx and 2-locular ovary. It is categorized as Endangered in view of its endemicity and habitat specificity. A key is provided for 30 species of the genus Graffenrieda that share the character of calyptrate flowers.Conclusions: This species is named in honor of the Colombian botanist Dairon Cárdenas López, a great collector and connoisseur of the Colombian Amazonian flora. It is so far known only from the type localities. The key to the species with calyptrate flowers included in this article allows 43% of the species in the genus Graffenrieda to be recognized, including four Centronia species.

A new species of frog of the genus Noblella Barbour, 1930 (Amphibia: Strabomantidae) from the Cordillera del Cóndor, Ecuador.

We describe a new species of leaflitter frog of the genus Noblella from southern Ecuador, province of Zamora Chinchipe. The new species is diagnosed from all its congeners by having one or two tubercles on the upper eyelids; distal phalanges strongly T-shaped; phalangeal formula on hands 2-2-3-3; phalangeal formula on feet 2-2-3-4-3; heel with a small subconical tubercle; disc on all toes with papillae; dorsum brown or brown with gray, with V-shaped inverted or scattered irregular darker marks. We include a detailed description of its osteology and a study of its phylogenetic relationships. Finally, we evaluate its conservation status and discuss the threats that are currently impacting at the type locality.

Two new species of Caribbiantes, with the redescription of the type species and a review of male genital patterns in Antillean Stenostygninae (Opiliones: Laniatores: Biantidae)

Caribbiantes cubanus Šilhavý, 1973, type species of genus Caribbiantes Šilhavý, 1973, is redescribed based on abundant material from the type locality, providing new data on its morphological variability and penis morphology. Two new species of Caribbiantes are described, C. obtusus sp. nov. and C. barbai sp. nov., based on additional specimens from central and eastern Cuba, for which we give information on their geographical distribution, intraspecific variability and habitat. A new diagnosis for Caribbiantes and updated geographic distributions of the included species are provided. The sexually dimorphic glandular structures on the metatarsus III of males for all Caribbiantes species are described. Male dimorphism, most evident in the robustness of the chelicerae, is reported for the studied species. The male genitalia patterns of Antillean stenostygnines species are described. The phylogenetic affinity of Antillean stenostygnines with Stenostygnus Simon, 1879, a genus from the north of South America, is discussed based on some morphological traits.

The Rediscovery of Noblella peruviana after More than 115 Years Helps Resolve the Molecular Phylogeny and Taxonomy of Noblella (Amphibia, Anura, Strabomantidae)

We revise the taxonomy of the frog genus Noblella on the basis of a molecular phylogeny. Previous studies recognized that Noblella is non-monophyletic, with one clade distributed from southeastern Peru to northeastern Bolivia and adjacent areas in Brazil and another clade distributed from northern Peru to Ecuador and southeastern Colombia. The lack of sequences from the type species Noblella peruviana prevented the investigation of its phylogenetic position and the status of related taxa. Our rediscovery after more than 115 years allowed for the inclusion of DNA sequences of Noblella peruviana obtained from specimens collected at the type locality in southeastern Peru. We inferred a phylogeny based on a concatenated dataset (three mitochondrial and two nuclear loci) using Bayesian and maximum likelihood methods. Our phylogeny corroborated the non-monophyly of Noblella and helped resolve the status of related taxa, including Psychrophrynella bagrecito, the type species of the genus Psychrophrynella (rediscovered after 42 years). We identified a clade containing N. peruviana, P. bagrecito, and other species of Noblella and Psychrophrynella distributed in southern Peru. Given that the name Noblella predates Psychrophrynella, we propose that Psychrophrynella should be considered a junior synonym of Noblella. The second clade contains species of Noblella distributed in Ecuador and northern Peru, including N. myrmecoides, which used to be the type species of the genus Phyllonastes. Consequently, we propose to reinstate the genus Phyllonastes to accommodate all species of Noblella distributed in Ecuador, northern Peru, southeastern Colombia, and adjacent areas in Brazil. We present an updated taxonomy including new combinations for 12 species and reinstatements for three species.

Two Palaearctic species of Orthonevra (Diptera: Syrphidae) under the name O. brevicornis

During studying European material of Orthonevra Macquart, 1829 the presence of two widespread species so far interpreted as Orthonevra brevicornis (Loew, 1843) has been discovered. One species occurs from Central Europe into Transcaucasia and agrees with the taxon O. brevicornis of Loew (1843). The second species, O. atlantica Żóralski & Van de Meutter sp. nov., has its main distribution range in the Atlantic parts of Europe and is described here. We provide a redescription of O. brevicornis and designate a neotype from the type locality (Poland, Posen [= Poznań] vicinity). The most important characters for distinguishing males of these two closely related species are in the structures of the terminalia. A diagnosis and identification key to European black-legged Orthonevra species is given as well as new sequences of the cytochrome c oxidase subunit I (COI) of mitochondrial DNA (mtDNA), also known as DNA barcodes, for most of the species of this group. Some new information and changes to the known ranges of Orthonevra species are provided as a result of this revisionary work: O. frontalis (Loew, 1843) is removed from the list of species occurring in Poland; O. gemmula Violovitsh, 1979 is confirmed from Hungary; O. incisa (Loew, 1843) is confirmed from its locus typicus (Poland, Posen vicinity); and O. montana Vujić, 1999 is reported from Poland and Austria for the first time and confirmed for Germany. An explanation for some previous records published as O. plumbago (Loew, 1840) is also given.

Intraspecific venom variation in the Iberian asp viper (Vipera aspis zinnikeri) across natural and intensive agricultural habitats

Snake venom composition varies at different levels. To date, comparative venom studies have seldom taken into account the role of habitat type in the occurrence of snake venom variation. Here we investigated the presence of venom variation across different populations of the Iberian asp viper (Vipera aspis zinnikeri) inhabiting two contrasting habitats: natural vs. intensive agricultural. We used shotgun proteomics to describe the protein composition of the venoms of six adults from two distinct localities. Furthermore, to test whether local conditions and habitat can alter venom composition in this taxon, we compared the SDS-PAGE profiles of 40 adult venoms from six populations, three in natural habitats and three in intensive agricultural environments. The venoms were composed of 21 toxin families, of which five (CTL, PLA2, VEGF, svSP, and svMP) comprised 69–82 % of each proteome. The relative abundances of toxin families varied considerably at inter- and intra-population levels. Linear regression performed on non-metric multidimensional scaling values showed a significant effect of locality of origin and habitat type on the differences detected between individual SDS-PAGE venom profiles. Our results suggest the presence of regional variation in V. a. zinnikeri venom, potentially reinforcing the role of local pressures in shaping snake venom composition. SignificanceThis work provides the first proteomic characterization of the venom of the Iberian asp viper, Vipera aspis zinnikeri, obtained by means of shotgun proteomics. The statistical analysis of 40 individual SDS-PAGE venom profiles highlights that venom variation in this taxon can be associated with geographical origin and habitat type of the area where each viper was collected. Our results suggest the presence of regional variation in V. a. zinnikeri venom, reinforcing the role that local pressures may play as drivers of snake venom variation.

Net zero initiative in U.S. beef and dairy systems: integrative on-farm recommendations for greenhouse gas reduction

Abstract Beef and dairy production systems play an important role in society, providing a variety of ecosystem services. U.S. beef and dairy production systems require being aligned with the global and national effort to stabilize the anthropogenic greenhouse gas (GHG) emissions in the atmosphere. This study adapted the nominal group technique framework to design a roadmap to achieving a net-zero GHG cattle supply chain in the U.S. with an emphasis on farm recommendations. Scientists with diverse expertise in sustainable beef and dairy production proposed, categorized, described, defined, and prioritized strategies that have the potential to significantly reduce GHG emissions, improve production system efficiencies, and promote sustainability. These strategies were presented to different stakeholders and classified according to the marginal GHG reduction, expected return on investment, and market readiness. Thus, strategies were defined for cow-calf and stocker, feedlot, and dairy operations, according to the characteristics of the cattle systems in the U.S. This net-zero roadmap presents a broad range of options for promoting sustainable cattle production in the U.S. Priority items for a research agenda to facilitate progress towards implementing this net-zero roadmap are described according to the dairy or beef production system and including the modulation of rumen fermentation, precision diet management, manure management, increasing animal and system efficiency, and genetic evaluation and selecting of efficient animals. The expected return on investment and market readiness of the proposed strategies depend on the technology type and system localization. Progress toward the net-zero goal depends on the widespread adoption of appropriate mitigation strategies. Future research programs must prioritize identified research needs to promote the wide adoption of the proposed strategies.