The chemical vapor deposition (CVD) method was employed to fabricate a two-dimensional (2D) MoS2 nanosheet and the related characteristics were measured. By using a new crystal Tm:Ca(Gd,Lu)AlO4 as laser medium, a laser-diode (LD) pumped dual-loss-modulated Q-switched laser with AOM and a MoS2 nanosheet was first presented as far as we know. The minimum pulse duration of 82 ns and the maximum peak power of 589 W were obtained under the modulation rate of 3 kHz. In comparison with the singly Q-switched laser with AOM or MoS2, the dual-loss-modulated Q-switched laser could generate shorter pulse width and higher peak power. The maximum compression ratio of pulse duration was 9.85 and the highest enhancement factor of peak power was 123. The experimental results hit a conclusion that 2D MoS2 nanosheet is potential in pulse laser at ∼2 µm and dual-loss-modulated Q-switching operation can compress the pulse duration and improve the peak power.