Twist Level Research Articles

Effect of yarn twist on tensile strength, abrasion and pilling resistance of plain-woven cotton fabric

The effect of twist on tensile strength, abrasion resistance, and pilling resistance of plain-woven fabric has been studied. The plain-woven fabric has been produced from open-end spun cotton yarn. The weft yarn has been produced at 4 different twist levels (900, 905, 910, and 915 TPM) and the effect of twist levels is also studied. To examine the effect of twist, four different samples of plain-woven fabric are produced from these yarns. From the ANOVA results, it is found that with an increment in twist level of the yarn, there is a significant increment in tensile strength, abrasion resistance, and pilling resistance of the fabric.

Investigation of Track Geometry Defects on a Heavy-Haul Railway Line

This paper presents an in-depth case study of a heavy-haul railway line in Sweden to analyze the twist and longitudinal level geometry defects. A linear model was applied to model the evolution of the amplitude of the longitudinal level defects and twist over time. Despite the effect of the defect shapes on the dynamic track loads, the amplitude of the defects still is the only criterion used for the assessment of geometry defect severity. The application of first- and second-order derivatives to capture information about the shape of defects was investigated in the case study. In addition, the RUSBoost algorithm was used to classify track sections into healthy and unhealthy sections using the imbalance class data set. In this algorithm, the standard deviation and the kurtosis of the geometry parameters were used as explanatory variables. Finally, the abnormal track geometry degradation patterns identified in the case study were explored in detail. The results of the analysis can be used directly in maintenance modeling and used for the purpose of maintenance scheduling.

A relative hairiness index for evaluating the securities of fiber ends in staple yarns and its application

Hairiness is a prominent property of staple yarns, but the existing evaluation parameters mainly describe the fiber ends already protruding out of yarn bodies. The potential fiber ends in yarns also play a crucial role in the performance of yarns in the subsequent processes and the resultant fabric quality. In our previous studies, maximum hairiness and its theoretical model have been proposed, which indicate the maximum fiber ends of a staple yarn having the potential to protrude out of yarn bodies and become hairy. On this basis, the relative hairiness index (RHI) is developed in this study to evaluate the fiber end tucking and securities of yarns. This index is treated as a ratio of the measured hairiness of sample yarns and the maximum hairiness of ring yarns in the same twist level and yarn count. A lower RHI indicates more fiber ends being tucked into yarn bodies, and a slower increment of the RHI with the increasing winding times represents more stable securities of fiber ends in yarns. The experimental results demonstrate that the RHI can directly reveal the effectiveness of different spinning parameters and methods in tucking and securing fiber ends; also, the changes of the RHI with increasing winding times visually present the stableness of fiber ends in various yarns experiencing abrasion, as well as predict the possibility of the potential fiber ends being pulled out to form hairiness during successive processes. The proposed RHI, therefore, provides a significant reference for the spinning process design and yarn quality control.

Twisting in improving processing of waste-derived yarn into high-performance reinforced composite

Waste cotton fibers was spun into yarns for potential use as high performance composites in order to recycle the ever-increasing waste textiles generation and solve the disposal problem. This study investigates the effects of different twist levels (550–1050 TPM), twist ratios (0.1–0.5 plied yarn twist to single yarn twist), and epoxy resin impregnation rates (0.2–1.0 cm/s) on tensile properties of single yarns, plied yarns, and yarn reinforced composites derived from waste cotton fibers. The detection of the failure mechanisms of the examined yarns was confirmed via SEM analysis and images captured by high-speed camera. The twist factor at low twist level (550 TPM), twist ratio (0.1) and impregnation rate (0.3 cm/s) were determined as optimum twist factors to produce plied yarn-reinforced composite with high tensile strength of 286 MPa. The optimum twist of single yarn reinforced composites was 0.76 times the optimum twist of the single yarn alone. Twisting significantly influenced the manufacturing of high-performance composites from waste-derived yarn. The obtained results provide a new paradigm for high value utilization of waste textile through conversion into high-performance composites.

LncRNA SNHG3 Promotes Gastric Cancer Cells Proliferation, Migration, and Invasion by Targeting miR-326.

The function and possible mechanism of lncRNA Small Nucleolar RNA Host Gene 3 (SNHG3) in GC have not been fully studied. The aim of our study was to investigate the role of SNHG3 in the proliferation, migration, and invasion of GC cell lines. The expressions of SNHG3, miR-326, and TWIST in GC9811-P GC cell lines were detected by RT-qPCR. Western blotting was performed to detect the protein levels of TWIST and EMT-related genes. Luciferase reporter gene analysis and RNA immunoprecipitation (RIP) analysis confirmed the interaction between lncRNA SNHG3, miR-326, and TWIST. CCK-8 and Transwell assays were performed to detect cell proliferation, invasion, and migration abilities. The results showed that lncRNA SNHG3 and TWIST were highly expressed in GC cell lines, while miR-326 was expressed to a low degree. Moreover, lncRNA SNHG3 knockdown or miR-326 overexpression significantly inhibited cell proliferation, migration, and invasion of GC cell lines. In addition, TWIST overexpression can reverse the inhibition of lncRNA SNHG3 knockdown or miR-326 overexpression on cell proliferation, migration, and invasion. In conclusion, lncRNA SNHG3 may promote GC progression through the miR-326/TWIST axis, which may provide a new diagnostic and prognostic biomarker for GC.

Autophagy modulates FSS-induced epithelial-mesenchymal transition in hepatocellular carcinoma cells.

Hepatocellular carcinoma is a highly fatal disease and threatens human health seriously. Fluid shear stress (FSS), which is caused by the leakage of plasma from abnormally permeable tumor blood vessels and insufficient lymphatic drainage, has been identified as contributing pathologically to cancer metastasis. Autophagy and epithelial-mesenchymal transition (EMT) are both reported to be involvedin cancer cell migration and invasion, but little has been revealed about the interaction between autophagy and EMT under a tumor mechanical microenvironment. Here, we identified that exposure to 1.4 dyne/cm2 FSS could promote the formation of autophagosomes and significantly increase the expressions of autophagy-related markers of beclin1 and ATG7, and the ratio of LC3Ⅱ/Ⅰin both of HepG2 and QGY-7703 cells. The FSS loading also elevated the levels of mesenchymal markers N-cadherin, Vimentin, Twist, Snail, and β-catenin, while the epithelial markers E-cadherin showed a decrease. Once the autophagy was blocked by 3-methyladenine (3-MA) or knocking ATG5 down, the occurrence of FSS-induced EMT was inhibited dramatically according to the expression and translocation of E-cadherin, N-cadherin, and β-catenin. Given the effect of EMT on cell migration, we observed that inhibition of autophagy could impede FSS-induced cell migration. Collectively, this study demonstrated that autophagy played a crucial role in FSS-induced EMT and cell migration in hepatocellular carcinoma.

Expression pattern and prognostic impact of glycoprotein non-metastatic B (GPNMB) in triple-negative breast cancer

Glycoprotein non-metastatic B (GPNMB) is a transmembrane protein overexpressed in numerous cancers including triple-negative breast cancers (TNBC). It has been linked to promote cancer aggressiveness and implicated as a novel target for GPNMB-expressing cancers. In current study, we aimed to explore the clinical significance of GPNMB in TNBC. Among 759 specimens, immunohistochemistry (IHC) exhibited GPNMB expressions were variable in different subtypes and significantly higher in TNBC. Kaplan–Meier analysis revealed GPNMB overexpression in TNBC was associated with worse prognosis especially distant metastasis (P = 0.020, HR = 2.515, CI 1.154–5.480). Multivariate analysis showed GPNMB expression was an independent prognostic factor in terms of recurrence and distant metastasis (P = 0.008, HR = 3.22, CI 1.36–7.61; P = 0.017, HR = 3.08, CI 1.22–7.74). In silico analysis showed high mRNA expression of GPNMB was associated with distant metastasis and GPNMB was overexpressed in TNBC. Furthermore, GPNMB positively correlated with epithelial–mesenchymal transition (EMT) regulators, mesenchymal marker vimentin, MMP and integrins. The protein levels of Twist and MMP2 were upregulated by GPNMB overexpression in TNBC cells. GPNMB-enhanced cell invasion was attenuated by broad spectrum MMP inhibitor (GM 6001) and the selective inhibitor of MMP-2 (ARP100). In summary, GPNMB expression is prevalent in TNBC and may be implicated as a prognostic biomarker in patients with TNBC.

Lovastatin Inhibits EMT and Metastasis of Triple-Negative Breast Cancer Stem Cells Through Dysregulation of Cytoskeleton-Associated Proteins.

Triple-negative breast cancer (TNBC) is more aggressive and has poorer prognosis compared to other subtypes of breast cancer. Epithelial-to-mesenchymal transition (EMT) is a process in which epithelial cells transform into mesenchymal-like cells capable of migration, invasion, and metastasis. Recently, we have demonstrated that lovastatin, a 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitor and a lipid-lowering drug, could inhibit stemness properties of cancer stem cells (CSCs) derived from TNBC cell in vitro and in vivo. This study is aimed at investigating whether lovastatin inhibits TNBC CSCs by inhibiting EMT and suppressing metastasis and the mechanism involved. In the present study, we found that lovastatin dysregulated lysine succinylation of cytoskeleton-associated proteins in CSCs derived from TNBC MDA-MB-231 cell. Lovastatin inhibited EMT as demonstrated by down-regulation of the protein levels of Vimentin and Twist in MDA-MB-231 CSCs in vitro and vivo and by reversal of TGF-β1-induced morphological change in MCF10A cells. Lovastatin also inhibited the migration of MDA-MB-231 CSCs. The disruption of cytoskeleton in TNBC CSCs by lovastatin was demonstrated by the reduction of the number of pseudopodia and the relocation of F-actin cytoskeleton. Combination of lovastatin with doxorubicin synergistically inhibited liver metastasis of MDA-MB-231 CSCs. Bioinformatics analysis revealed that higher expression levels of cytoskeleton-associated genes were characteristic of TNBC and predicted survival outcomes in breast cancer patients. These data suggested that lovastatin could inhibit the EMT and metastasis of TNBC CSCs in vitro and in vivo through dysregulation of cytoskeleton-associated proteins.

Differentiated pre-adipocytes promote proliferation, migration and epithelial-mesenchymal transition in breast cancer cells of different p53 status.

Breast cancer progression and metastasis are associated with stromal cells in the tumor microenvironment. Adipocytes are the most abundant cells surrounding breast stromal tissue, promote tumor progression through the induction of Epithelial-to-Mesenchymal Transition (EMT) which is negatively regulated by tumor suppressor protein p53. In this study aimed to investigate the role of p53 in the progression of breast cancer after mature adipocyte-conditioned medium (CM) application. The proliferative effect of CM obtained from differentiated pre-adipocytes were assessed by MTS assay. 20% CM increased cell proliferation in breast cancer cells, T-47D (mutant p53) and MCF-7 (wild-type p53). The migration and invasion capacity were evaluated by scratch and transwell assays, respectively. CM significantly enhanced migration and invasion capacity in T-47D compared to MCF-7. Gene and protein expressions were detected by qRT-PCR and Western Blot analysis, respectively. CM markedly increased expression levels of Cyclin D1, PI3K, MMP9, Snail and Twist in T-47D compared to MCF-7. However, CM did not change E-Cadherin level in T-47D while downregulated in MCF-7 cells. Also, the protein levels of NFκB p65, p-Akt, Snail, and Vimentin were upregulated in both cells. Overall, the findings highlight how the p53 status affects mature adipocyte-mediated proliferation, migration, and aggressive behavior of breast cancer cell lines. Targeting the tumor microenvironment may represent a promising approach for preventing breast cancer progression and metastasis.

Processing and Tensile Properties of Twisted Core-Shell Yarns Fabricated by Double Nozzle Electrospinning Device

In this study, a double nozzle electrospinning device consisting of a take-up/twister unit applied to develop core-shell structured nanofibrous yarns with potential applications in the production of functional textile materials. The process was performed by introducing a pre-electrospun core yarn into the electrical field between two oppositely charged nozzles, where nanofibers cover it by a certain arrangement owning to the twisting procedure. Herein, the main goal was to investigate how the core and shell structures influenced by process parameters, can contribute to the ultimate mechanical properties of the electrospun core-shell yarn. Accordingly, the same solution of nylon 66/formic acid was used for electrospinning of both core and shell nanofibers. The response surface methodology (RSM) was applied to study the effect of twisting rate, take-up speed, and twist amount of core yarn (called pre-twist) on the morphological and tensile properties of electrospun core-shell yarns. SEM images confirmed that the nanofibers were assembled surround the core with a certain angle to the axis to form a twisted core-shell yarn. The take-up speed caused considerable effects on the yarn diameter by changing the number of fibers formed in the triangle zone of the electrospinning process. The diameter of the core-shell yarns decreased significantly by increasing the twist rate and take-up speed. Improvements in tensile stress were generally realized at low twist levels and higher take-up speeds. The contribution of the core on the mechanical behavior of core-shell yarn was also considered in detail. The electrospun core-shell yarns showed superior (∼37 %) max stress when using a core yarn with low pre-twist amounts.

Small molecule inhibitor of nicotinamide N-methyltransferase shows anti-proliferative activity in HeLa cells

The anti-proliferative effects of 5-methylquinolinium (5MQ) of nicotinamide N-methyltransferase (NNMT) have not been previously investigated on a cervical cancer cell line. NNMT is a metabolic enzyme that is correlated with tumour progression and metastasis. 5MQ is a small molecule inhibitor of NNMT. 0.1–500 μM of 5MQ was tested on the HeLa epithelial cervical cancer cell line. Cell viability was assessed with the MTT test. TWIST, ZEB1, SERPIN1, SIRT1, CD16, mRNA and various protein expression levels were analysed with Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western Blotting, respectively. 5MQ significantly inhibited HeLa cell proliferation in a concentration and time-dependent manner. Increased cell shrinkage, loss of cellular adhesions and apoptotic bodies were observed in HeLa cells after 5MQ treatment. Following treatment with 5MQ, ZEB1, SIRT1, CD16 mRNA levels were increased while TWIST and SERPIN1 mRNA levels were reduced. Expressions of oncogenic proteins phospho-Akt and SIRT1 were decreased. 5MQ can effectively inhibit HeLa cell proliferation without apparently affecting HEK-293 cell proliferation. IMPACT STATEMENT What is already known on this subject? NNMT is a cytosolic enzyme involved in tumour progression, metastasis and treatment resistance. It was overexpressed in many human malignancies. 5-amino-1-methylquinolinium (5MQ) is a novel small molecule inhibitor of NNMT that has shown promising results in the treatment of obesity and in senescent muscle regeneration. 5MQ has not been tested on the HeLa cervical cancer cell line, previously. What do the results of this study add? In this study, 5MQ was tested on the HeLa cervical cancer cell line for the first time and the molecular changes associated with 5MQ treatment were analysed. 5MQ demonstrated significant anti-proliferative activity on HeLa cells, which displayed morphological signs of apoptosis. Treatment of HeLa cells with 5MQ led to an increase in ZEB1, SIRT1 mRNA while TWIST mRNA was decreased. Phospho-Akt and Sirtuin1 protein expressions were decreased. What are the implications of these findings for clinical practice and/or further research? 5MQ can effectively inhibit HeLa cell proliferation without apparently affecting HEK-293 cell proliferation. 5MQ treatment was associated with a decrease in the expression of phospho-Akt and Sirtuin1 proteins, both of which have been reported to maintain tumour progression. 5MQ can further be investigated and modified for anti-cancer therapy.

Twist activates miR-22 to suppress estrogen receptor alpha in breast cancer.

TWIST1 (Twist) is a basic helix-loop-helix transcription factor that is overexpressed in many cancers and promotes tumor cell invasion, metastasis, and recurrence.In this study, we demonstrate that Twistupregulates expression of microRNA 22 (miR-22) which, in turn, downregulates estrogen receptor alpha (ER) expression in breast cancer. Initial analysis of miR-22 and Twist expression in a panel of breast cancer cell lines showed a direct correlation between Twist and miR-22 levels with miR-22 being highly expressed in ERnegative cell lines. Overexpressing Twist caused increased miR-22 levels while downregulating it led to decreased miR-22 expression. To characterize the upstream promoter region of miR-22, we utilized rapid amplification of cDNA ends and identified the transcription start site and the putative promoter region of miR-22. Mechanistically, we determined that Twist, in combination with HDAC1 and DNMT3B, transcriptionally upregulates miR-22 expression by binding to E-boxes in the proximal miR-22 promoter. We also established that miR-22 causes an increase in growth in 3D but not 2D cultures. Importantly, we observed a directcorrelation between increased breast cancer grade and Twist and miR-22 expression. We also identified two potential miR-22 binding sites in the 3'-UTR region of ER and confirmed by promoter assays that miR-22 regulates ER expression by binding to both target sites. Theseresults reveal a novel pathway of ER suppression by Twist through miR-22 activation that could potentially promote the ERnegative phenotype in breast cancers.

Effect of twist level on the mechanical performance of S-glass yarns and non-crimp cross-ply composites

High modulus/high strength continuous fibres are used extensively for manufacturing textile preforms, as a reinforcement, for composites due to their excellent specific properties. However, their brittle behaviour and tendency to separate easily into individual filaments or bundles can lead to damages during manufacturing processes such as weaving and braiding. Thus, the critical step in the development of an optimal yarn for textile-reinforced composites is to find an optimum twist, which results in a minimum loss of properties of the composite laminates, while maintaining good processability and sufficient strength for textile and/or composite manufacturing. In this study, twist level has been varied to improve the handling and tensile properties of S-glass yarns (i.e. tensile strength). Varying levels of yarn twist (15–40 twists metre−1) were employed to study its impact on the tensile properties (i.e. tensile strength, modulus, elongation at break etc.). Furthermore, the effect of twist on the tensile properties of non-crimp cross-ply composites produced via vacuum infusion process was studied. It was observed that mechanical performance (i.e. tensile strength properties) of twisted yarns is improved up to 30 twists metre−1 while it is deteriorated at 40 twists metre−1. At yarn level, the experimental results were compared with theoretical estimations utilizing existing models for twisted yarns properties. Discrepancies were observed between experimental and theoretical results especially for high level of twist. The tensile strength and elongation of S-glass cross-ply composites at all levels of twist were higher compared to the composite laminates manufactured by using non-twisted yarns. At composite level, the experimental results were also computed employing rule of mixture and good agreement was observed between experimental and predicted results.

Expression of Twist, Slug and Snail in esophageal squamous cell carcinoma and their prognostic significance.

Esophageal cancer is one of the most common types of malignancy worldwide. At present, surgical resection is the main treatment for esophageal cancer, but recurrence and distant metastasis are the main causes of mortality. The transcription factors Twist, Slug and Snail regulate epithelial-mesenchymal transition and thereby participate in tumor invasion and metastasis. The aim of the present study was to investigate the expression of Twist, Slug and Snail in esophageal squamous cell carcinoma (ESCC) and their prognostic significance. The expression of Twist, Slug and Snail in 229 paraffin-embedded ESCC and matched normal mucosal tissues was detected by immunohistochemistry. The expression differences of Twist, Slug and Snail in the ESCC and normal tissues were compared by χ2 test, and the associations between the three proteins and the clinicopathological parameters of ESCC were analyzed. The expression levels of Twist, Slug and Snail in 29 fresh frozen ESCC and matched normal mucosal tissues were detected by reverse transcription-quantitative PCR. The correlations among Twist, Slug and Snail in ESCC were examined by Pearson's correlation analyses. In addition, single factor and multivariate Cox regression analyses were used to analyze the influence of Twist, Slug and Snail on the prognosis of ESCC. Twist was found to be highly expressed in ESCC. The difference of Slug expression in ESCC was associated with differentiation degree, TNM stage and vascular invasion, but no significant association was observed between Snail expression and any clinicopathological parameters. In ESCC, there were significant differences in protein expression between Twist and Snail, and Slug and Snail. The mRNA expression level of Twist in ESCC was significantly higher than that in normal esophageal mucosa. However, the mRNA expression of Slug in normal esophageal mucosa was higher than that in ESCC, and the mRNA expression levels of Twist and Snail were positively correlated in ESCC. Kaplan-Meier analysis of 229 patients with ESCC revealed that Snail influenced the overall survival, as did the co-expression of Twist and Snail. Nerve invasion was also identified as an independent factor affecting the progression-free survival of ESCC. The results indicate that Twist is highly expressed, Slug may be a tumor suppressor, and Snail is an independent prognostic factor in ESCC. Twist and Snail are positively correlated, and the simultaneous inhibition of Twist and Snail protein expression may be beneficial for prolonging the overall survival of patients with ESCC.

Study on the Properties of Cotton Yarn with Modified Yarn Path in Ring Spinning

Spinning triangle is a critical area that influences the physical and mechanical properties of yarn. Previous researchers have done several works related to spinning triangle by modifying yarn path primarily considering only one side diagonal yarn path meaning that either left or right diagonal yarn path. The others used single yarn count and twist level on different variety of raw materials. This research was produced cotton ring yarns by varying count (36 Ne, 40 Ne) and twist level (1014 TPM, 1080 TPM) using right and left diagonal paths in Z-twist direction. The yarns were tested and it was observed that 25%, 0.34%, 37% and 25% reduction in hairiness, CVm%, thin place and thick place respectively with slight decreasing of tenacity in left diagonal path yarn. In right diagonal path yarn there is 3%, 16%, 59% and 14% increasing of yarn hairiness, CVm%, thin place and thick place respectively with 10% reduction of tenacity. Therefore, the left diagonal path has a significant improvement of ring spun yarn quality in terms of physical properties. On the other hand, the right diagonal path yarn were deteriorated all the physical and tensile properties of ring spun yarn. This is occurring due to the mismatching of right diagonal path with Z-twist direction.

Recombinant Human IL-11 Promotes Lung Adenocarcinoma A549 Cell Growth and EMT through Activating STAT3/HIF-1α/EMT Signaling Pathway.

Interleukin-11 (IL-11) could promote invasion and metastasis of cancer cells, however, its mechanism is unclear. This study aimed to investigate the effects of recombinant human IL-11 (rhIL-11) on lung cancer cell metastasis and growth. Human lung cancer cell, A549, was cultured and subcutaneously injected into mice to establish Xenograft tumor models. Tumor models were divided into control, rhIL-11 transplantation (250 μg/kg/day), and rhIL-11 transplantation (500 μg/kg/day) group. Tumor volumes were recorded and measured 6 times. Hypoxia- Inducible Factor 1α (HIF1α), snail, slug, Signal Transducers/Activators of Transcription-3 (STAT3), E-cadherin, twist, and vimentin levels were evaluated using western blot and Real-Time PCR (RT-PCR). Sizes of subcutaneous tumors increased following measurement time. rhIL-11 treatment significantly enhanced HIF1α and STAT3 expression in rhIL-11 treatment groups compared to the control group (p<0.05). However, no remarkable differences were discovered between rhIL-11 (250 μg/kg/day) and rhIL-11 (500 μg/kg/day) group (p>0.05). rhIL-11 treatments significantly increased twist, and slug expressions compared to control group (p<0.05), especially for rhIL-11 (500 μg/kg/day) treatment, which triggered significantly higher effects on twist and slug expressions compared to those in the control group (p<0.05). Vimentin and snail mRNA levels were significantly up-regulated and E-cadherin level was significantly down-regulated in rhIL-11 treatment groups compared to the control group (p<0.05). Meanwhile, rhIL-11 at a dosage of 500 μg/kg/day triggered remarkably higher effects on vimentin, snail, and E-cadherin expressions compared to those in rhIL-11 (250 μg/kg/day) group (p<0.05). rhIL-11 transplantation promoted growth and Epithelial-Mesenchymal Transition (EMT) of A549 cells, which might be associated with STAT3/HIF-1α/EMT signaling pathway activation.

Magnesium Isoglycyrrhizinate Induces an Inhibitory Effect on Progression and Epithelial-Mesenchymal Transition of Laryngeal Cancer via the NF-κB/Twist Signaling.

BackgroundMagnesium isoglycyrrhizinate (MI) was extracted from roots of the plant Glycyrrhiza glabra, which displays multiple pharmacological activities such as anti-inflammation, anti-apoptosis, and anti-tumor. Here, we aimed to investigate the effect of MI on the progression and epithelial–mesenchymal transition (EMT) of laryngeal cancer.MethodsForty laryngeal cancer clinical samples were used. The role of MI in the proliferation of laryngeal cancer cells was assessed by MTT assay, Edu assay and colony formation assay. The function of MI in the migration and invasion of laryngeal cancer cells was tested by transwell assays. The effect of MI on apoptosis of laryngeal cancer cells was determined by cell apoptosis assay. The impact of MI on tumor growth in vivo was analyzed by tumorigenicity analysis using Balb/c nude mice. qPCR and Western blot analysis were performed to measure the expression levels of gene and protein, respectively.ResultsWe identified that EMT-related transcription factor Twist was significantly elevated in the laryngeal cancer tissues. The expression of Twist was also enhanced in the human laryngeal carcinoma HEP-2 cells compared with that in the primary laryngeal epithelial cells. The high expression of Twist was remarkably correlated with poor overall survival of patients with laryngeal cancer. Meanwhile, our data revealed that MI reduced cell proliferation, migration and invasion and enhanced apoptosis of laryngeal cancer cells in vitro. Moreover, MI decreased transcriptional activation and the expression levels of NF-κB and Twist, and alleviated EMT in vitro and in vivo. MI remarkably inhibited tumor growth and EMT of laryngeal cancer cells in vivo.ConclusionMI restrains the progression of laryngeal cancer and induces an inhibitory effect on EMT in laryngeal cancer by modulating the NF-κB/Twist signaling. Our finding provides new insights into the mechanism by which MI inhibits laryngeal carcinoma development, enriching the understanding of the anti-tumor function of MI.

Investigating the effects of some process parameters on the quality of coarse compact yarn produced from carded roving

This project work aimed to investigate the interactive effects of carded roving parameters (i.e., roving hank and twist) and break draft of ring frame on the quality of compact yarn such as evenness, strength and hairiness. However, in this work, compact spun yarns produced from carded roving hanks of 0.70Ne and 0.90Ne while the roving twist per meter inserted at two levels (i.e., 40 and 45) for each of hank. Moreover, the break draft values of ring frame kept at three levels (i.e., 1.15, 1.25 and 1.35) for each type of roving. However, 100% cotton compact yarns of 24Ne and 32Ne produced for this experimental investigation. However, results showed that yarn unevenness (U%) and imperfection index (IPI) values of compact yarn decreased for the finer roving hank (0.90Ne) irrespective of yarn fineness. It was also noticed that low twisted roving improved yarn evenness during coarser yarn (i.e., 24Ne) production. Regarding the count strength product (CSP) value, increasing levels of roving twist deteriorated the CSP results in general. Trend of CSP results also revealed that finer roving produced higher levels of CSP results for comparatively finer yarn production. As far as yarn hairiness is concerned, it was observed that finer roving (0.90Ne) having higher levels of twist reduced yarn hairiness irrespective of yarn fineness without any exception. Finally, it can be said that this study will help the researchers for comprehending the influences of the process parameters during the production of compact yarn from carded roving.

Impact of temperature on survival and spinal development of golden pompano Trachinotus ovatus (Linnaeus 1758)

This study investigated the impact of water temperature on survival, skeletal development and the molecular mechanism of temperature-induced spinal deformity in golden pompano Trachinotus ovatus. Fish larvae were subjected to three temperatures (26, 29 and 32 °C) from 0 to 32 days post-hatch (DPH). On 32 DPH, significant higher growth rate was observed in fish cultured at 32 °C, but the survival rate and RNA/DNA ratio were significantly lower than fish culture at 26 and 29 °C. At the end of this study, the highest spinal deformity rate was observed in fish cultured at 32 °C. The ambient temperature should be kept at or below 29 °C for the best growth performance. The levels of BMP2, BMP4, BMP5, Sox9, twist and vimentin were significantly regulated by ambient temperature. High temperature may up-regulate the expression of a series of signaling factors during bone formation and down-regulate the expression of sox9, affecting the production of ECM components, thereby affecting the development of bone and cartilage and leading to spinal deformities. The results from the present study provide new evidence to understand the molecular mechanism of spinal deformities and bone development golden pompano.

Osteoglycin silencing exerts inhibitory effects on myocardial fibrosis and epithelial/endothelial-mesenchymal transformation in a mouse model of myocarditis.

Osteoglycin (Ogn), a class III SLRP member with multiple glycosylation sites, has been proposed to be engaged in cardiac dysfunction and adverse remodeling in human heart failure following myocardial infarction. However, the underlying mechanism remains to be elucidated. Thus, we sought to define the role of Ogn in regulation of the Wnt pathway on myocardial fibrosis and epithelial/endothelial-mesenchymal transformation (EMT/EndMT) in mice with myocarditis. The pathological changes are observed, while hematoxylin-eosin staining and picric acid Sirius red staining were conducted in successfully constructed myocarditis mouse models. Immunohistochemistry and enzyme-linked immunosorbent assay were adopted to determine Ogn and β-catenin levels and serum procollagen propeptide concentrations in the mouse myocardial tissues, respectively. Expression of Ogn and Wnt signaling pathway-related factors were measured by reverse transcription quantitative polymerase chain reaction and western blot assay, cell viability by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell cycle distribution and apoptosis by flow cytometry. We saw indicative pathological changes accompanied by many Ogn and β-catenin positive cells and increased serum procollagen propeptide, in the mouse myocardial tissues. Loss function assays showed reduced levels of Ogn, β-catenin, LRP6, TGF-β1, Twist, FSP-1, α-SMA and higher levels of E-cadherin and VE-cadherin, together with decreased proliferation rate, as well as increased apoptosis rate, indicating that the Wnt signaling pathway, proliferation were inhibited while apoptosis was enhanced with upon gene silencing. Coherently, depletion of Ogn inhibits myocardial fibroblasts proliferation and EMT/EndMT while facilitating myocardial fibroblasts apoptosis in myocarditis through the Wnt signaling pathway, thus serving as an intervention target for the molecular treatment of myocarditis.