Natural convection heat transfer in cavities commonly occurs in various engineering problems. Specifically, the problem of a differentially heated cavity with oscillatory boundary conditions arises in the design of energy-saving strategies or energy evaluations within the building sector. This work addressed numerically and experimentally the natural convection in a full-scale room subjected to diurnal heating and nocturnal cooling. The experiment was instrumented with temperature sensors and an air velocity sensor, inspired by works reported in the literature. The numerical model involved solving the equations governing natural convection, considering turbulent bi-dimensional and three-dimensional flow in a transient state. This research allowed the selection of an appropriate turbulence model and evaluated the performance of the two-dimensional and three-dimensional approaches in simulations. Results showed that the k-omega SST model performed best in predicting velocity, while the standard k-omega model performed best in predicting temperature for the two-dimensional simulation, and the RNG k-epsilon model performed best for the three-dimensional simulation. Additionally, the three-dimensional simulation more accurately reproduced the chaotic fluctuations observed in experimental velocity data, demonstrating up to 30 % better estimation during periods of high velocities. The study identified the reversal of convective cells in the cavity, with an approximate duration of 2 h and 40 min in a real case. This study contributes to improving the understanding of turbulent natural convection in a differentially heated cavity with oscillatory boundary conditions. These findings have significant implications for various environmental and engineering applications, including the design of thermally comfortable buildings.