Lead-bismuth eutectic (LBE) is a liquid with low Prandtl number and high thermal conductivity, generally used as the coolant in lead-cooled fast reactors of Generation-IV. The traditional Reynolds analogy is not appropriate for LBE, hence the theoretical analysis and experimental measurements are studied to interpret the flow and heat transfer of LBE in this article. In this way, a single-rod experiment on flow and heat transfer for LBE is performed and a new empirical correlation is fitted. To analyze the behavior of flow and heat transfer for low Prandtl number fluids, and also to validate the turbulence model and new correlation in the hexagonal rod bundles. The CFD simulations are performed for the XJTU 7-rod bundle, the KIT 19-rod bundle, and the ENEA 37-rod bundle based on ANSYS Fluent. The convective heat transfer coefficient of LBE, the temperature of heating wall at different sub-channels, and the temperature non-uniformity are investigated in this paper, to reveal the flow and heat transfer characteristics of LBE. The results show that the deviations of the section averaged Nusselt number are in 25%, and the deviations of the local temperature of LBE and heating wall are in 10℃. Grid spacers will result in a 30% rise in the section averaged Nusselt number, which will improve the turbulent intensity and heat transfer. Temperature non-uniformity is reduced as the Peclet number is increased. The validations demonstrate that the new empirical correlation provides accurate convective heat transfer coefficient predictions for LBE flow and heat transfer in hexagonal rod bundles with a relative derivation in 20%, which can be used as a reference for additional LBE simulations and experiments.