Abstract This paper investigated the film-cooling effectiveness of diffusion slot holes in a turbine nozzle guide vane. The pressure-sensitive paint measurement technique was employed to obtain the film-cooling effectiveness at a density ratio of DR = 1.5. The mainstream Reynolds number based on the axial chord length and the exit velocity was 6 × 105. The mainstream turbulence intensity was approximately 3.7%. Three diffusion slot hole geometries with cross-sectional aspect ratios (ASs) of 2.3, 3.4, and 4.9 were tested and compared with a typical fan-shaped hole. The experiments were performed at three typical hole row locations on the pressure surface (PS) and suction surface (SS). The average blowing ratios varied from M = 0.5 to 2.5. The results showed that throughout the blowing ratio range, on the PS, a substantially higher film-cooling effectiveness than the fan-shaped hole is always obtained from the diffusion slot hole with a large aspect ratio (AS = 4.9); on the SS, the diffusion slot hole with a small AS (AS = 2.3). The influence of hole row positioning is inconsistent for diffusion slot holes with different ASs. The diffusion slot hole is less affected by the PS when the AS is moderate and less affected by the SS when the AS is large. The film-cooling effectiveness of the diffusion slot holes is basically the lowest where the PS has a maximum concave curvature and the highest where the SS has a large favorable pressure gradient.