In water bodies, where the bottom is visible to the sensor, remotely sensed data can produce erroneous estimates of water column constituents. The bottom impact depends upon depth and turbidity of the water body, color and composition of the bottom, and the wavelengths of spectral measurement. In the case of clear water, the maximum separability of the composite upwelling signals from bottom at varying depths was recorded in wavelength region between 400 and 900 nm. For water with an algal suspension of 478 □g/l, the maximum separability of upwelling signals was within 500 and 600 nm. In both clear and algal waters, bottom at depths greater than 50 cm did not impact the upwelling signals in wavelength range beyond 744 nm.