The impact of train-induced vibrations originating from underground tunnels has emerged as a significant environmental issue within urban areas. A multitude of studies, encompassing both numerical analyses and experimental investigations, have been undertaken to explore and address this concern. In the experimental tests, the dynamic characteristics of tunnels and soil has generally been studied using physical models with a single tunnel under a 1 g (gravity) environment. However, twin tunnels are commonly constructed in the field, and the stress of the soil is unrealistically simulated in 1 g models. In this paper, the dynamic characteristics of twin tunnels were studied by centrifuge testing. In this paper, results are presented from scaled model tunnel tests conducted at 50 g for four different conditions: a single tunnel, vertical twin tunnels with 60 mm spacing, horizontal twin tunnels with 60 mm and 120 mm spacing. Train load and sweep load were applied by a parallel pre-stressed actuator at the tunnel invert. Accelerometers were employed to gauge the dynamic response of both the tunnels and the surrounding soil. The experimental results illustrate that the interaction between twin tunnels has a clear amplification effect on the dynamic response of the loading tunnel, with the maximum peak particle acceleration (PPA) increasing by up to 43 %. This amplification phenomenon is reduced as the space between the twin tunnels is increased. The experimental results also indicate that the interaction between twin tunnels has a comparatively lower impact on the dynamic response of the surrounding soil when contrasted with the dynamic response of the tunnels themselves.