This paper describes an application of PIV (particle image velocimetry) and two-tracer PLIF (planar laser-induced florescence) techniques to optimize the in-cylinder flow and to visualize two fuels distribution simultaneously for developing a fuel stratification engine. This research was carried out on a twin-spark four-valve SI engine. The PIV measurement results shows that a strong tumbling flow was produced in the cylinder as the intake valves were shrouded. The flow exhibited a symmetrical distribution in the plane perpendicular to the cylinder axis from the early stage of intake until the late stage of compression. This flow pattern helps to stratify the two fuels introduced from separate ports into two regions laterally. The stratification of fuels was observed visually by the two-tracer PLIF technique. During the PLIF measurement, two tracers, 3- pentanone and N, N-dimethylaniline (DMA), were doped into two fuels, hexane and iso-octane, respectively. Their fluorescence emissions were separated by two optical band-pass filters and recorded by a single ICCD camera simultaneously via an image doubling system. The PLIF measurement result shows that two fuels were well stratified.