Tulsi Oil Research Articles

In vitro antibacterial activity of antibiotics and plant essential oils against Escherichia coli MTCC443 supported through the molecular docking and pharmacokinetics study.

Most of the Escherichia coli turned into serious pathogens or developed antibiotic resistance, mainly due to their ability to show different phenotypic traits. In order to overcome the resistance to these antibiotics, the use of essential oils (EOs) is of great significance against highly pathogenic microorganisms. This study has been made to compare the in vitro antibacterial activity and further validated the same through the molecular docking study of 13 antibiotics such as ciprofloxacin, chloramphenicol, erythromycin, ampicillin, cefotaxime, rifampicin, kanamycin, vancomycin, streptomycin, penicillin, nalidixic acid, trimethoprim, and polymyxin, and 10 EOs such as garlic, tulsi, neem, clove, thyme, peppermint, coriander, tea, lavender, and eucalyptus against the target protein (DNA gyrase) of E. coli MTCC443. E. coli Microbial Type Culture Collection 443 was found to be highly sensitive to ciprofloxacin (zone of inhibition [ZOI], 2.5cm ±0.1) and chloramphenicol (ZOI, 1.8cm ±0.1), whereas garlic oil (ZOI, 5.5cm ±0.1) and coriander oil (ZOI, 4.4cm ±0.1) were found comparatively most effective. Further, the in silico investigation observed the same; ciprofloxacin (binding affinity: -7.2kcal/mol) and chloramphenicol (binding affinity: -6.6kcal/mol). Penicillin (binding affinity: -4.2kcal/mol) and polymyxin (binding affinity: -0.3kcal/mol) were found to be least effective against the tested microbe, whereas vancomycin (binding affinity: +0.8kcal/mol) had no effect on it. Garlic (binding affinity: -7.8kcal/mol), coriander (binding affinity: -6.8kcal/mol), peppermint (binding affinity: -6.2kcal/mol), and neem (binding affinity: -6.2kcal/mol) oil exhibited the potent antibacterial activity against E. coli MTCC443, whereas thyme (binding affinity: -6.1kcal/mol), tea tree (binding affinity: -4.9kcal/mol), and tulsi (binding affinity: -3.8kcal/mol) oil were observed moderately effective. Eucalyptus (binding affinity: -2.9kcal/mol) and lavender (binding affinity: -2.8kcal/mol) oil were found to be the least effective among all the oils tested. The pharmacokinetics and networking were performed to the pharmacology of the potential compounds.

ANTIMICROBIAL ACTIVITY OF TULSI EXTRACT – A Review

The therapeutic value of tulsi is enormous. Tulsi has also been demonstrated in studies to be beneficial for diabetes by lowering blood glucose levels. The identical research demonstrated a noteworthy decrease in overall cholesterol levels when using Tulsi. According to another study, Tulsi's antioxidant qualities are what provide it a positive impact on blood glucose levels. The best treatment for severe acute respiratory syndrome is Rama tulsi. Its leaf juice relieves cough, bronchitis, fever, and colds. Another ear drop that is utilized is tulsi oil. Malaria can be cured with tulsi. It works well for cholera, headaches, sleeplessness, hysteria, and indigestion. Every day, millions of people consume fresh Tulsi leaves. Tulsi, the "queen of herbs," has been used for generations. KEYWORDS: Ocimum sanctum, antibacterial action, leaf extract, good diffusion, and Ocimum tenuiflorum

Larvicidal activity of essential oils of Haridra and Tulsi against Aedes aegypti and Anopheles larvae

An attempt is made in the present study to analyse the larvicidal activity of Haridra and Tulsi against Aedes and Anopheles larvae. Larval bioassays were carried out at different concentrations 50-500ppm. The larval mortality of fourth instar larvae of A. aegypti and Anopheles after 24h of treatment was observed separately in control, 50, 62.5, 100, 150, 250, 300 and 500 ppm concentrations. Based on the Probit analysis, Significant effect (Estimate adh_conc = 3.069, 95% C.I. [2.082, 4.057], z = 6.093, p<.05) of Haridra oil extract concentration for Aedes (adh_conc) on the Probit response or mortality was observed. The median lethal Concentration, LC50= 166.353, 95% C.I. [134.745, 203.268], 90% Lethal Concentration, LC90 = 435.065, 95% C.I. [328.259, 712.462] were recorded. Statistical analysis revealed that significant effect (Estimate adt_conc = 3.369, 95% C.I. [2.229, 4.509], z = 5.791, p<.05) of Tulsi oil extract on Aedes larvae concentration (adt_conc) on the Probit response or larvae mortality (adt_mort) was observed. The median Lethal Concentration, LC50= 105.051, 95% Confidence Intervals (C.I). [81.451, 127.428], 90% Lethal Concentration, LC90 = 252.242, 95% C.I. [199.229, 376.772] of essential oil of Tulsi oil against Aedes aegypti larvae was recorded. No mortality of Aedes larvae was recorded in the control group. Essential oil of Haridra at different concentrations shows a significant effect (Estimate anh_conc = 2.038, 95% C.I. [1.180, 2.896], z = 4.655, p<.05 against the mortality of Anopheles Larvae on the Probit response or mortality. The median Lethal Concentration, LC50 = 124.823, 95% Confidence interval CI. [84.002, 164.336], 90% Lethal Concentration, LC90 = 530.978, 95% C.I. [349.474, 1333.827] of essential oil of Curcuma longa against Anopheles larvae was recorded. (Estimate ant_conc = 2.257, 95% C.I. [1.135, 3.200], z = 4.694, p<.05) of Tulsi oil - extract on Anopheles Larvae concentration (ant_conc) on the Probit response or mortality (ant mort) was observed. Median Lethal Concentration, LC50 = 339.419, 95% Confidence Interval (C.I.) [259.239, 533.727], 90% Lethal Concentration, LC90 = 1254.519, 95% C.I. [717.77, 4574.12] of essential oil of Tulsi oil against Anopheles larvae. Larvicidal activity of both the plants against Aedes larvae at different concentration was significant. But LC50 and LC90 values of Ocimum sanctum (Tulsi) against mortality of Aedes aegypti were less as compared to Curcuma longa (Haridra). It revealed that Tulsi showed better larvicidal activity against Aedes larvae as compared to Haridra. It was also observed that LC50 and LC90 values of Haridra against Anopheles larvae were less than Tulsi. It revealed that Haridra showed better larvicidal activity against Anopheles larvae as compared to Tulsi.

Preparation and Evaluation of Herbal Toothpaste

Targeted regions in India include the Japanese Chain, Eastern Himalayas, Western Ghats, and Andaman & Nicobar Island. There are 45000 ancient medicinal plant species there. However, only 3000 plants have been officially identified as having potential for health. Practitioners from antiquity employ over 6000. India is the world's top producer of medicinal plants and is known as the "World Botanical Garden." Toothpastes are complicated abrasive and surfactant combinations; anticavity humectants (to avoid dryness and improve the pleasant mouth feel); agents like fluoride; tartar control components; pH buffers; and binders, which give things form and consistency. Natural components in herbal toothpaste are more socially acceptable than Due to their safety, chemical-containing toothpastes are commonly used in oral dental care today. and effectiveness in lowering and effectiveness in avoiding tooth caries and other dental conditions that this Creation is susceptible. We incorporate plant extracts like peppermint oil, ginger oil, and oil, Tulsi oil, clove oil, and neem leaf oil; camphor and honey are also included. These extracts have wound-healing, anti-ulcer, anti-caries, anti-bacterial, and as well as some special qualities like anti-fungal and anti-cancer. Preparing, evaluating, and comparing Lab Made Products is the primary goal of the current activity. Toothpaste with commercial herbal natural toothpaste. During the investigation, commercial The quality of herbal toothpastes like Vicco has been assessed. every marketed Herbal toothpaste manufactured in laboratories and tooth pastes made from herbs that had been tested complied. In accordance with the criteria established by the Bureau of Indian Standards. Detect F-, Pb, As, and stability research. The evaluation of all the parameters revealed that the lab-created formulation is roughly equivalent and hardly ever produces greater results than advertised formulation. Hence It was determined that the chosen Labmade formulation was of high grade.

Formulation and development of herbal ingredients loaded Emulgel

The present study aims to develop and evaluate an emulgel that contains curcumin, clove oil, and basil oil for the treatment of mouth ulcers. Emulgels have become a potentially effective method of delivering hydrophobic medications. Menthol was used as a penetration enhancer, which also provides cooling effect to ulcers, whereas clove oil also acts as a penetration enhancer. The herbal medicines such as clove oil, tulsi (basil) oil, curcumin, and menthol have very good antiulcer activity. Clove oil has analgesic and antioxidant activity whereas, basil oil obtained from ocimum basilicum plants has antimicrobial and analgesic activity. Curcumin is obtained from curcuma longa which acts as anti-inflammatory and as antiseptic, whereas menthol provides cooling to the ulcers or oral sore. Five formulations were prepared and evaluated for viscosity, spreadability, extrudability, diffusion tests, and short-term stability. At the end of the five-hour investigation, the optimised formulation (E3G3S5T2) had showed drugs (clove, basil, and curcumin) release of 79 ± 3.2%, 72.22 ± 3.1%, and 72.07 ± 4.8% respectively. From the study, it can be suggested that the emulgel can be delivered and used topically and has better penetrating capabilities.

Benefit of Tulsi for General and Dental Medicine

Tulsi has a significant amount of therapeutic value. Studies have shown that Tulsi can help people with diabetes by lowering the amount of glucose in their blood. Tulsi is a powerful medicine that can treat severe cases of acute respiratory syndrome. The juice extracted from its leaves is said to be effective in treating coughs, bronchitis, fever, and colds. The ear drop that is utilised is also made from tulsi oil. Tulsi is beneficial in the treatment of malaria. It is highly efficient against conditions such as cholera, indigestion, headaches, hysteria, and insomnia. Every day, millions of people all around the world consume the fresh leaves of the Tulsi plant. Tulsi is particularly useful for supporting the functions of the heart, blood vessels, liver, and lungs, and it can also help manage blood pressure and blood sugar levels. In nearly all of the old ayurvedic writings that have been preserved, OS has been praised highly for the excellent medicinal powers it possesses. It has a taste that is pungent and astringent, and the effect that it has on the body is one that is hot, light and dry. It is said that the seeds of this plant have a chilling effect on the humans who consume them. Tulsi has various different therapeutic characteristics that can be found in its roots, leaves, and seeds. It has been given upon us by Mother Nature a very rich botanical richness, and throughout the United States you can find a wide variety of plant species growing in their natural habitats. Traditional medicine, modern medicine, modern drugs, nutraceuticals, food ingredients, folk medicine, pharmaceuticals and the chemicals that are used to make synthetic drugs all get most of their drugs from plants. Plants used for medical purposes have a significant economic worth in many parts of the world. Tulsi, which is also known as Ocimum sanctum Linn, is a too useful plant that is used in India's traditional medical practises. In the next round of studies, the healing properties of this plant will be looked into, since it has been shown to help with a broad range of oral and systemic conditions

In-vitro trials to evaluate the efficacy of green microparticles for the control of cattle tick, Rhipicephalus (Boophilus) microplus

ABSTRACT Ticks are haematophagous ectoparasites that infest all domestic animals and humans. They gain medical and veterinary significance due to the variety of aetiological agents that they can transmit. Acaricides have played a pivotal role in tick control until the surge of resistance to most of the commonly employed acaricides. Alternate tick control strategies have since gained significant attention and semiochemical assisted tick control is one such strategy. Herein, we attempted to employ an eco-friendly strategy to lure and kill ticks. Sustained-release microparticles in the form of porous calcium alginate beads incorporating the attraction sex pheromone, 2,6-DCP, and tulsi oil were prepared, and their efficacy was assessed using petri-dish bioassay. There was 100% mortality of the larvae and partially fed males exposed to these microparticles after 24 hours of exposure.

Environmentally Sound Approach for Fabrication of Antibacterial/Anti-UV/Anti-crease and Fragrant Denim Fabrics

A green approach for imparting multifunctional properties namely antibacterial, anti-UV, anti-crease and fragrance property to indigo dyed cotton denim fabrics using environmentally benign finishing, hosting and bioactive agents was investigated. In the current work, pre-loading of MCT-βCD, as a hosting agent, via ester-crosslinking using CA/SHP, as formaldehyde-free crosslinking system using pad-dry-cure method, followed by post-treatment with selected bio active agents namely Clove oil, Lavender oil, Tulsi oil and Vanillin R were carried out to add new, durable and desirable functional properties. The obtained results demonstrated that the variation in the efficacy and durability of the imparted functional properties is governed by extent of loading the modified βCD, number and availability of hydrophobic hosting-cavities, type and chemical constituents of the guest molecule and its extent of inclusion into the created cavities via formation of host-guest complex, as well as its retention or release during the washing process. The remarkable improvement in antibacterial activity against the pathogenic bacteria followed the decreasing order: Tulsi≥ Clove> Vanillin> Lavender>> None. The obtained results also demonstrated that, the imparted anti-UV capability of post-loaded denim samples follows the descending order: Vanillin>Tulsi>Clove>Lavender>>None. The imparted functional properties showed a high durability even after 15 washes.

Antimicrobial activity of some Essential oils and Extracts from Natural sources on Skin and Soft tissue infection causing microbes: An In-vitro Study

Introduction: Skin and soft tissue infections (SSTIs) constitute the diverse group of infections with varied clinical presentation and severity. SSTI’s are the most common infections treated in hospital today posing diagnostic and therapeutic challenges. Natural herbs have been widely used as a potential source of therapeutic substances all over the world. A study was conducted to determine the antimicrobial effect of some natural extracts on important SSTI causing pathogens. Materials and methods: In this study the antimicrobial potential of essential oil of Cinnamomum zeylanicum, Eugenia caryophyllata, oil of Cymbopogon citratus, Melaleuca alternifolia, Mentha piperita, Ocimum sanctum and extracts of Curcuma longa, Azadirachta indica and Cassia fistula were analysed against common skin and soft tissue infections (SSTIs) causing microorganisms. The organisms include methicillin resistant Staphylococcus aureus (MRSA), methicillin sensitive Staphylococcus aureus (MSSA), Streptococcus pyogenes, Streptococcus agalactiae, Cutibacterium acnes, E.coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Bacteroides fragilis. The antimicrobial assay was performed by agar punch well method with different concentrations of essential oil and extracts. High performance liquid chromatography (HPLC) was performed in order to identify the active constituents present in the extracts. Further a formulation was made, with the powdered extract (neem, turmeric, bark of golden shower) and cinnamon oil as the chief component to look for any probable synergetic activity. Results and discussion: In this study all the essential oils showed good antimicrobial activity than the crude extracts. Significant activity against the Gram negative organisms was exhibited by all the products used in the study. Cinnamon and Clove oil had an excellent activity against all the organism whereas tea tree, mint and Tulsi oil showed better results against the Gram positive organisms including acne vulgaris causing organisms. HPLC results emphasised the presence of eugenol and cinnamaldehyde in clove and cinnamon oil respectively. A significant zone of inhibition was observed from the formulation prepared. Hence we indicate that the natural products used in this study have therapeutic properties and therefore could be considered as an alternative medication in treatment of SSTIs.

Erratum to: Incorporation of Fluconazole and Ocimum Sanctum Oil in Soft Denture Liners to Treat Biofilms of Candida albicans Associated with Denture Stomatitis

Purpose: To investigate the In Vitro activity of incorporated antifungal agents like Fluconazole and Ocimum sanctum oil (Tulsi) in the denture soft liners to reduce the risks associated with the biofilms of Candida albicans.Materials and Methods: In the current study, the minimum inhibitory concentration (MIC) of two antifungal agents namely Fluconazole and O. sanctum (Tulsi oil) against C. albicans (ATCC 10231) was evaluated to examine their effect in reducing the count of candida and its biofilms. Analysis of physical properties such as surface roughness and hardness of soft denture liners (test and control) were also performed. The effect of both antifungal agents was also observed on cell morphology of candida cells using scanning electron microscopy (SEM).Results: Studies confirmed MIC value for fluconazole and O. sanctum oil as 600 and 400 µg/ml respectively. Surface hardness and roughness of soft denture material (test) remained unaltered. Finally, SEM studies also proved the effectiveness of incorporated antifungal agents on the cell morphology of C. albicans at their respective MIC values.Conclusions: This approach allows the prolonged drug release in the oral cavity which simultaneously treats the injured denture bearing tissues and also the infection, biofilms of candida without compromising on their physical properties. These studies are significant and have tremendous medicinal and therapeutic relevance. Keywords: Antifungal Agents; Biofilms; Candida; Scanning Electron Microscope; Soft Liners; Hardness; Surface Roughness; Minimal Inhibitory Concentration; Denture Stomatitis.

Incorporation of Fluconazole and Ocimum Sanctum Oil in Soft Denture Liners to Treat Biofilms of Candida albicans Associated with Denture Stomatitis

Purpose: To investigate the In Vitro activity of incorporated antifungal agents like Fluconazole and Ocimum sanctum oil (Tulsi) in the denture soft liners to reduce the risks associated with the biofilms of Candida albicans.Materials and Methods: In the current study, the minimum inhibitory concentration (MIC) of two antifungal agents namely Fluconazole and O. sanctum (Tulsi oil) against C. albicans (ATCC 10231) was evaluated to examine their effect in reducing the count of candida and its biofilms. Analysis of physical properties such as surface roughness and hardness of soft denture liners (test and control) were also performed. The effect of both antifungal agents was also observed on cell morphology of candida cells using scanning electron microscopy (SEM).Results: Studies confirmed MIC value for fluconazole and O. sanctum oil as 600 and 400 µg/ml respectively. Surface hardness and roughness of soft denture material (test) remained unaltered. Finally, SEM studies also proved the effectiveness of incorporated antifungal agents on the cell morphology of C. albicans at their respective MIC values.Conclusions: This approach allows the prolonged drug release in the oral cavity which simultaneously treats the injured denture bearing tissues and also the infection, biofilms of candida without compromising on their physical properties. These studies are significant and have tremendous medicinal and therapeutic relevance.

Tulsi: The Queen of Medicinal Herbs

Tulsi has got the great medicinal value. Studies have also shown Tulsi to be effective for diabetes, by reducing blood glucose levels. The same study showed significant reduction in total cholesterol levels with Tulsi. Another study showed that Tulsi's beneficial effect on blood glucose levels is due to its antioxidant properties. The Rama Tulsi is the effective remedy for the severe acute Respiratory Syndrome. Juice of its leaves gives relief in cold, fever, bronchitis and cough. Tulsi oil is also used as the ear drop. Tulsi helps in curing malaria. It is very effective against indigestion, headache, hysteria, insomnia and cholera. The fresh leaves of Tulsi are taken by the millions of people every day. For over the centuries Tulsi (the queen of herbs) has been known for its remarkable healing properties. Modern scientific research offers impressive evidence that Tulsi reduces stress, enhances stamina, relieves inflammation, lowers cholesterol, eliminates toxins, protects against radiation, prevents gastric ulcers, lowers fevers, improves digestion and provides a rich supply of antioxidants and other nutrients. Tulsi is especially effective in supporting the heart, blood vessels, liver and lungs and also regulates blood pressure and blood sugar. Recent studies suggest that Tulsi may be a COX-2 inhibitor, like many modern painkillers, due to its high concentration of eugenol (1-hydroxy-2- methoxy-4- allylbenzene). The anti-flu property of Tulsi has been discovered by medical experts across the world quite recently. Tulsi improves the body's overall defence mechanism including its ability to fight viral diseases.

Ex vivo comparative evaluation of efficacy of disinfecting ability of garlic oil, neem oil, clove oil and tulsi oil with autoclaving on endodontic K files, tested against aerobic bacteria

Aim: This study aims for comparative evaluation of efficacy of disinfecting ability of garlic oil, neem oil, clove oil and tulsi oil with autoclaving on endodontic k files tested against aerobic and anaerobic bacteria. Materials and Methods: 50 endodontic K-file were used in oral cavity as sample size, which were immersed into 4 different herbal oils and were checked for its disinfecting ability with three different methods. Results: In this study garlic oil is more effective against aerobic bacteria when compared to neem oil, clove oil and tulsi oil. Conclusion: Garlic oil can be used as an alternative method to autoclave against aerobic bacteria but autoclave is a gold standard disinfecting method for sterilization.

A critical review on traditional herbal plant ‘tulsi’

The medicinal value of Tulsi is fantastic. Studies have also found that Tulsi has been beneficial in lowering blood glucose levels in diabetes. In the same analysis, overall cholesterol levels with Tulsi were greatly decreased. Another research found that its antioxidant effects had a positive effect on blood glucose. The Rama Tulsi is the powerful treatment for extreme acute airborne syndrome. Juice from her leaves provides frost, flu, bronchitis and cough relief. As an ear drop, tulsi oil is used too. In cure of malaria, Tulsi aids. It is particularly effective against indigestion, fever, hysteria, sleeplessness, cholera. Millions of people each day taking the new leaves of Tulsi. Tulsi, the medicinal queen, is renowned for its excellent healing properties for many generations. Modern science study has demonstrably demonstrated the reduction and resilience of stress, inflammation reduce, cholesterol reduction, contaminants removal, radiation safety, gastric ulcers avoidance, fevers reduction, metabolism and a high degree of antioxidant and other nutrients. This research often offers remarkable outcomes. Tulsis protects the heart, blood vessels, liver and lungs in particular and controls blood pressure and blood sugar. Recent studies indicate that Tulsi, like many modern painkillers, may be a COX-2 inhibitor due to its strong eugenol concentration (1-hydroxy-2methoxy-4-allylbenzene). Tulsi is also promising to guard against toxicity and cataracts. The oil from the Karpoora Tulsi is used primarily for herbal washing. It also uses the oil against bacteria and insects. Tulsi can keep the dreaded swine or H1N1 flu in the background, but can also lead to rapid recovery of a person suffering from Ayurvedic practises. “Medical researchers worldwide have recently uncovered Tulsi's anti-flu property. Tulsi strengthens the global system of defence, including its capacity to suppress infectious disease.

Bioefficacy of fungicides, plant extracts and essential oils against Exserohilum turcicum (Pass.) Leonard and Suggs causing Northern leaf blight of maize

Eight fungicides, ten botanicals and ten essential oils were taken for their in vitro evaluation against Exserohilum turcicum (Pass.) Leonard and Suggs incited of Northern Leaf Blight of Maize. Among all the tested fungicides (Azoxystrobin 23%, Thiophenate methyl 75%, Propiconazole 25%, Tebuconazole 25.9%, Captan 50%, Zineb 75%, Cymoxanil 8%+Mancozeb 64% and Zineb 68%+Hexaconazole 4%), propiconazole 25% showed maximum inhibition of mycelial growth (92.22%) under in vitro conditions at 5ppm concentration. Among all the tested botanicals Heena (Lawsonia inermis) was found to be most effective in inhibiting mycelial growth (71.11%) at 10% concentration. Lemon tulsi oil was found best in inhibiting mycelial growth (71.30%) of Exserohilum turcicum, among all the tested essential oils at 50ppm concentration. Fungicides, plant extracts and essential oils showing good results under in vitro conditions were tested under glasshouse conditions. Foliar spray of Propiconazole 25%EC@0.1% showed reduced disease incidence (23.33%) compared to control (46.33%). Application of 5% of Heena extracts reduced the disease incidence (26.67%). Plants when sprayed with Lemon tulsi oil showed reduced disease incidence (24.17%). The study reveals that not only the fungicides but also the natural plant extracts can greatly contribute to reduce the incidence of disease because of the antifungal compounds present in plants.

COMPARATIVE STUDY OF ANTIMICROBIAL ACTIVITY OF LEMONGRASS (CYMBOPOGON CITRATUS), CLOVE (SYZYGIUM AROMATICUM), AND TULSI (OCIMUM) ESSENTIAL OILS AGAINST FOODBORNE PATHOGENS

Objective: The present work aims to compare the essential oils (EOs) of Lemongrass (Cymbopogon citratus), Clove (Syzygium aromaticum), and Tulsi (Ocimum sp.) having antimicrobial activity and to find the most effective EO against different types of foodborne microorganisms.
 Methods: The EOs were investigated for its antimicrobial activity against different types of Gram-negative and Gram-positive bacteria such as Escherichia coli, Micrococcus luteus, Staphylococcus aureus, and Bacillus cereus and fungal strains such as Aspergillus niger, Candida albicans, Chaetomium globosum, and Penicillium funiculosum by agar well diffusion method. The antimicrobial actions of these EOs were evaluated by calculating the zone of inhibition.
 Results: Of the three EOs used in the study, the effect of clove oil was found greater against all the microorganisms followed by lemongrass and tulsi EOs. All the microorganisms used in the study were found sensitive to clove EO, wherein the highest zone of inhibition was observed in A. niger (41.56±5.05 mm) and P. funiculosum (40.34±4.83 mm), and C. globosum (39.53±1.69 mm) and smallest in S. aureus (9.77±0.93) and E. coli 1(11.07±0.52). Moreover, it was also found that EOs exhibit more sensitivity toward Gram-positive bacteria than Gram-negative bacteria.
 Conclusion: The successful effectiveness of EO can play an important role in resolving the major problem of human health arising due to the use of chemical preservative. It was found that EO of clove oil was the most effective against fungal and bacterial strains than lemongrass and tulsi EOs. Furthermore, clove oil is less costly than lemongrass and tulsi oil; it can be more economic and promising to be used as a food preservative against foodborne pathogens.

A review on: Holy basil and mediterranean basil (Genus Ocimum): The unique medicinal plant

In the present review on Tulsi, an effort has been done to Medicinal properties of Tulsi. The therapeutic uses of plant are considered as safe, inexpensive & efficient as their ease of availability. The basil plant consumed herbs, Ocimum genus in plant family Lamiaceae. Tulsi to be effective for diabetes, The Rama Tulsi is the effective remedy for the severe acute Respiratory Syndrome. Juice of its leaves gives relief in cold, fever, bronchitis and cough. Tulsi oil is also used as the ear drop. Tulsi helps in curing malaria. It is very effective against indigestion, headache, hysteria, insomnia and cholera. The fresh leaves of Tulsi are taken by the millions of people every day. Tulsi reduces stress, enhances stamina, relieves inflammation, lowers cholesterol, eliminates toxins, protects against radiation, prevents gastric ulcers, lowers fevers, improves digestion and provides a rich supply of antioxidants and other nutrients. Tulsi is especially effective in supporting the heart, blood vessels, liver and lungs and also regulates blood pressure and blood sugar. Recent studies suggest that Tulsi may be a COX-2 inhibitor, like many modern painkillers. Tulsi improves the body's overall defence mechanism including its ability to fight viral diseases. It is good source of vitamin A and C, Calcium, Zinc, Iron, Chlorophyll and it possess antibacterial, insecticidal properties. In different parts of the world, several types of basil are cultivated, some of the widely used varieties can be categorized in two groups – holy basil and Mediterranean basil.

Antimicrobial Coating of Cotton Twill Tape with Neem Oil, Eucalyptus Oil and Tulsi Oil for Medicinal Application

Antimicrobial Coating of Cotton Twill Tape with Neem Oil, Eucalyptus Oil and Tulsi Oil for Medicinal Application

An in vitro Comparative Evaluation of Efficacy of Disinfecting Ability of Garlic Oil, Neem Oil, Clove Oil, and Tulsi Oil with autoclaving on Endodontic K Files tested against Enterococcus faecalis.

Aim To comparatively evaluate the efficacy of disinfecting ability of garlic oil, neem oil, clove oil, and tulsi oil with autoclaving on endodontic K files tested against Enterococcus faecalis.Materials and methods Fifty endodontic K files were exposed to the test micro-organism and checked for its disinfecting ability using three different methods.Result Garlic oil, clove oil, tulsi oil and autoclave showed considerable effectiveness against E. faecalis except neem oil.Conclusion Garlic oil, clove oil and tulsi oil are an effective disinfectant and can be used as an alternative to autoclaving against the test micro-organism.Clinical Significance Herbs and herbal extracts are a natural and harmless way of controlling infection. These products are readily available and comparable to gold standard, thus can have its applications in rural India.How to cite this article Hugar S, Patel PM, Nagmoti J, Uppin C, Mistry L, Dhariwal N. An in vitro Comparative Evaluation of Efficacy of Disinfecting Ability of Garlic Oil, Neem Oil, Clove Oil, and Tulsi Oil with autoclaving on Endodontic K Files tested against Enterococcus faecalis. Int J Clin Pediatr Dent 2017;10(3):283-288.

Antinociceptive activity of Tulsi Amrit (A Polyherbal Formulation) in selective pain induced models in rats

Ocimum sanctum commonly known as Tulsi has been used for thousands of year in the ayurveda for its diverse healing properties. This study was conducted to evaluate the antinociceptive activity of tulsi oil (Tulsi Amrit) in pain induced rat models . The rats were divided into 4 Groups of 6 animals each (n=6). Group 1 – served as control, Group 2- was treated with standard drug piroxicam and Group 3 an Group 4 – were treated with test substance at two dose levels. The models selected to study the effect on nociception were hot plat assay, cold plate test in rats. The tusi oil demonstrated significant ( P < 0.0001) antinociceptive activity at the doses (100 and 200mg/kg body weight P.O) tested compared to standard piroxicam 20mg/kg. The tulsi oil (Tulsi amrit) was found to have a significant ( P < 0.0001) inhibitory effect on hot plate at both the doses as well on cold plate. The study reveals antinociceptive activity of Tulsi Amrit.