Abstract
Purpose: To investigate the In Vitro activity of incorporated antifungal agents like Fluconazole and Ocimum sanctum oil (Tulsi) in the denture soft liners to reduce the risks associated with the biofilms of Candida albicans.Materials and Methods: In the current study, the minimum inhibitory concentration (MIC) of two antifungal agents namely Fluconazole and O. sanctum (Tulsi oil) against C. albicans (ATCC 10231) was evaluated to examine their effect in reducing the count of candida and its biofilms. Analysis of physical properties such as surface roughness and hardness of soft denture liners (test and control) were also performed. The effect of both antifungal agents was also observed on cell morphology of candida cells using scanning electron microscopy (SEM).Results: Studies confirmed MIC value for fluconazole and O. sanctum oil as 600 and 400 µg/ml respectively. Surface hardness and roughness of soft denture material (test) remained unaltered. Finally, SEM studies also proved the effectiveness of incorporated antifungal agents on the cell morphology of C. albicans at their respective MIC values.Conclusions: This approach allows the prolonged drug release in the oral cavity which simultaneously treats the injured denture bearing tissues and also the infection, biofilms of candida without compromising on their physical properties. These studies are significant and have tremendous medicinal and therapeutic relevance.
Highlights
Denture soft liners are widely used for restoring the health of abused or inflamed tissues in the oral cavity which is brought about by the hard denture materials by providing cushioning and evenly distributing the load on the oral tissues during mouth movements
We investigated the effect of these inclusions on the hardness and surface roughness of the soft liner
A biofilm forming strain C. albicans (ATCC 10231) was selected for antifungal assays to mimic the characteristics of oral microflora
Summary
Denture soft liners are widely used for restoring the health of abused or inflamed tissues in the oral cavity which is brought about by the hard denture materials by providing cushioning and evenly distributing the load on the oral tissues during mouth movements. These liners surface harbor and support the adhesion, colonization of several microbes, especially pathogens like Candida albicans that result in lesion formation in denture wearers [1]. We wish to state a noteworthy observation here that in comparison with other plant extracts, O. sanctum oil has not been exploited thoroughly for prosthodontics purposes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
