Tubliseal EWT Research Articles

Dimensional changes of endodontic sealers-An in vitro model simulating a clinical extrusion scenario during 18 months.

To examine the dimensional changes of endodontic sealers during 18 months using three-dimensional (3D) surface scanning and subtraction radiography in a novel in vitro sealer-extrusion model. Fifty endodontically instrumented acrylic teeth were randomly allocated to five groups (n = 10) filled with Apexit Plus, AH Plus, BioRoot RCS, TubliSeal EWT, and gutta-percha (control). Freshly mixed sealers were intentionally extruded during obturation. All teeth were immersed in a physiologic solution for up to 18 months. Blinded 3D surface scans (resolution: ~10 μm) and digital radiographs of the teeth were obtained at baseline (immediately after obturation); and then after 1 week, and at 1, 3, and 18 months. For blinded assessment of sealer dimensional change, 3D models and radiographs were superimposed using specific software. Volumetric differences, root mean square (RMS), and area change from subtraction radiography measured at each period within each sealer group were thereafter calculated. Repeated measures analyses were done with Bonferroni adjustment for multiple comparisons; standard errors, p values, and 95% confidence intervals (CI) were reported. Analyses of the volumetric data confirmed significant, progressive material loss for Apexit Plus when compared to the other investigated sealers or the control group (p ≤ 0.02). Immersion period significantly influenced the volumetric dimensional changes of Apexit Plus already after 1 month (p < 0.01). For TubliSeal EW, the effect of the immersion period on the dimensional changes was noted after immersion for 3 months (p ≤ 0.02), while for BioRoot RCS this was evident only at 18 months (p < 0.01). Same trends were noted for the RMS data, whereas progressive dimensional changes using subtraction radiography only revealed significant changes for Apexit Plus (p = 0.01). The largest dimensional changes were shown by Apexit Plus, followed by Tubliseal EWT and BioRoot RCS. AH Plus remained stable throughout 18 months.

The flow rate of endodontic sealers in various consistencies

Introduction. The flow of endodontic sealer (ES) is the property that characterizes its velocity along the certain surface and depends on the friction force. The aim of this research was to measure the flow rate of three zinc oxide eugenol based ES?s in various consistencies exposed to the load of 2 kg. Material and methods. The experimental group included prepared samples of sealers protocoled by ADA specification No. 57 A) Endomethasone N in liquid:powder ratio of 1:5, 1:6, 1:7 (standard), 1:8 and 1:9 according to the manufacturer brochure depending on the clinical situation; B) Roth 801 as 1:7 (standard) and 1:8 mixtures C) Tubliseal EWT as standard preparation (base-catalyst 1:1). A volume of 0.05 ml sealer was spread on the glass plate and upon applying the load of 2 kg the diameter of sealer was measured. In the control group the sealer samples were loaded only by the weight of glass plate (0.1kg). Results. Based on measured diameters of sealers, all of them satisfied ADA requirements for the flow (d&gt;20mm) (Endomethasone - 20.7-27.8 mm; Roth 801- 29.6-30.0 mm; Tubliseal -39.9 mm). The thin consistency of sealers (1:5, 1:6) showed significantly higher flow than standard mixture (1:7) (p &lt; 0.05). Conclusion. Tubliseal EWT sealer showed the highest flow rate, significantly different than standard mixtures (1:7) of Endomethasone N and Roth 801.

Evaluation and comparison on the wettability of three root canal sealers after three different irrigant activation techniques: An in vitro study.

Background:Wettability of root canal sealers to the root canal wall plays a significant role in the attainment of a fluid-tight seal.Aims:This study aimed to evaluate and compare the wettability of three different root canal sealers on the root canal walls after three different irrigant activation techniques using the contact angle meter.Subjects and Methods:Thirty-six single-rooted mandibular premolars after decoronation and cleaning and shaping up to size #35 were randomly divided into three groups with 12 samples in each group (n = 12) based on the different activation techniques: Group I (passive irrigation with side-vented needle), Group II (manual dynamic agitation), and Group III (passive ultrasonic irrigation). Root segments were split longitudinally and were subdivided into three subgroups based on the root canal sealers tested with four samples in each subgroup (n = 4): Subgroup A (Tubliseal EWT), Subgroup B (AH Plus), and Subgroup C (Endosequence BC sealer). A controlled volume droplet of sealer was placed on each specimen and was subjected to the contact angle meter.Statistical Analysis:Data were statistically analyzed using the one-way ANOVA and Tukey’s post hoc test at a significance level of 0.05 (P < 0.05).Results:A statistically significant difference was seen among all the groups and subgroups with the highest contact angle value for Group III and Subgroup C and the least value for Group I and Subgroup A.Conclusions:Passive irrigation with side-vented needle and Tubli-Seal EWT showed better wettability on dentin.

Evaluation of Antimicrobial Efficacy of Root Canal Sealers againstEnterococcus faecalis:A Comparative Study

The study aimed to evaluate the efficacy of root canal sealer's antimicrobial activity against Enterococcus faecalis. Root canal sealers with the brands Endoflas FS, AH Plus, and Tubli-Seal EWT were selected. Enterococcus faecalis organisms' zone of inhibition was measured. Tukey post hoc tests and one-way analysis of variance (ANOVA) were used to compare the data among study groups and within its group. The data were said to be statistically significant with an established p-value less than 0.05. The maximum zone of inhibition (23.20 ± 1.202 mm) was shown by Endoflas FS sealer; AH Plus and Tubli-Seal showed the inhibition zone of (18.42 ± 1.023) and (16.88 ± 0.962) respectively, following Endoflas FS. The ANOVA test showed p < 0.0001, which is highly statistically significant. AH Plus and Endoflas FS showed a statistically significant difference of 0.04 and 0.001 in between groups, and there was no statistically significant difference in the Tubli-Seal group from Tukey post hoc test. The study showed that Endoflas FS sealer has a significant antimicrobial effect against E. faecalis. In clinical situation, despite perfect che-momechanical root canal preparation, persistence of microorganisms may reinfect the root canal. Therefore, endodontic root canal sealers play a major role in the eradication of bacteria. The polymicrobial nature of endodontic infection plays a main role during the usage of endodontic sealer with antimicrobial agents, which in turn reduces the failure of endodontic treatment.

AN INVITRO COMPARATIVE EVALUATION OF FRACTURE RESISTANCE OF ENDODONTICALLY TREATED TEETH OBTURATED WITH RESILON AND GUTTA-PERCHA

Abstract Aim: To compare and evaluate in-vitro the fracture resistance of endodontically treated teeth obturated with resilon &amp; epiphany sealer and guttapercha using different sealers. Epoxy resin based sealer AH-plus and zinc oxide eugenol based sealer-TubliSeal (EWT). Methodology: sixty four human single rooted maxillary anterior teeth, cleaned stored in 0.9% saline. All the teeth were decoronated to root length 14mm and bucco-lingual diameter of 5-7mm, After access openings teeth were instrumented using K3.06 up to final apical size 30/.06 and randomly allocated into 4 experimental groups (n=16 per group). Group 1(Control group): teeth were instrumented but not obturated, Group 2: Resilon cones and epiphany SE-sealer. Group 3: guttapercha cones and epoxy based sealer AH plus. Group 4: guttapercha and Tubli seal EWT. Coronal seal was done using IRM cement. Each of the specimens were tested for fracture resistance by instron universal testing machine. Results: Higher fracture resistance values were observed for group 2 (Resilon &amp; Epiphany SE sealer) followed by group 3(Guttapercha &amp; AH Plus sealer) and group 4(Guttapercha &amp; TubliSeal EWT) when compared to group1 (control-instrumented but not obturated). Conclusion: filling the root canals with contemporary polymer based root canal obturating system- Resilon increased the in vitro fracture resistance of endodontically treated teeth.

In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus.

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms, E. faecalis, C. albicans, and S. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity against E. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity against C. albicans and S. aureus compared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity against E. faecalis, C. albicans, and S. aureus.

A Comparative in vitro Evaluation of Vertical Root Fracture Resistance of Endodontically Treated Teeth using Different Obturating Materials

ABSTRACT Aim To compare the vertical root fracture resistance of endodontically treated teeth obturated using different obturating materials. Materials and methods Decoronated roots of 75 single canal lower second premolar teeth were divided randomly into 5 groups. The first group (Group I) served as a negative control. The remaining four Groups were cleaned and shaped using ProTaper Endodontic rotary files and obturated with guttapercha + TubliSeal EWT sealer (Group II), ThermaFil + AH Plus sealer (Group III), RealSeal + RealSeal sealer (Group IV) and GuttaFlow obturating system (Group V). The roots were then embedded in acrylic resin blocks and subjected to vertical load until fracture with a spreader in a universal testing machine and the forces were recorded. Results Fracture resistance of group I &gt; group IV &gt; group V &gt; group III &gt; group II, which were statistically significant. Conclusion Obturation of roots with resin based obturation material—RealSeal had increased VRF resistance compared to the gutta-percha obturation systems. Clinical significance Obturation of the root canals with resin based obturation system—Resilon reinforces the fracture resistance of endodontically treated teeth and contributes to the overall success of the endodontic treatment. How to cite this article Carlos RB, Makam S, Yaragonda VK, Murali H, Tyagi A, Rose R. A Comparative in vitro Evaluation of Vertical Root Fracture Resistance of Endodontically Treated Teeth using Different Obturating Materials. World J Dent 2014;5(2):113-117. Acronyms and abbreviations VRF: Vertical root fracture; ANOVA: Analysis of variance.

The effect of immediate and delayed post-space preparation using extended working time root canal sealers on apical leakage

Background/purposeThe purpose of this study was to compare the effect of different post-space preparation time intervals on the apical sealing ability of filling material using two different extended setting time root canal sealers. Materials and methodsA total of 100 single-canal teeth were incrementally cleaned with crown-down instrumentation using K3 rotary nickel–titanium instruments and randomly divided into four groups. There were 30 teeth in the experimental groups (Groups 1–3) and 10 teeth in the control group (Group 4). We obturated the teeth using a warm gutta-percha vertical compaction technique with pulp canal sealer (Group 1), Tubli-Seal EWT (Group 2), and pulp canal sealer EWT (Group 3). We then stored all the specimens in 100% humidity at 37°C for 1 week. We used heated pluggers to create post space at three different intervals postobturation (i.e., immediately after obturation, on Day 3, and on Day 7), leaving 5-mm gutta-percha filling at the apices. We then immersed the teeth in 2% methylene blue solution for 72 hours, and finally sectioned them longitudinally into two halves. The amount of apical dye leakage was measured under a stereomicroscope. ResultsThe results showed no significant difference (P > 0.05) in dye leakage between the standard and extended working time root canal sealers. Apical leakage tended to increase more severely in Group 1 at the three different intervals was considered. ConclusionExtended working time root canal sealers do not affect microleakage results using a warm gutta-percha vertical compaction technique. The sealing ability of extended working time root canal sealers on high heat conditions is as good as the standard working time root canal sealer during post-space preparation of different intervals.

A Comparative Evaluation of the Vertical Root Fracture Resistance of Endodontically Treated Teeth using Different Root Canal Sealers: An in vitro Study

The objective of this study is to evaluate the vertical root fracture resistance of endodontically treated teeth obturated with - Tubli-Seal EWT/Gutta-percha, AH Plus/Gutta-percha, Epiphany SE sealer/Epiphany point. Sixty-five single rooted premolars were decoronated and root length was 14 mm for each specimen. Fifty five teeth were enlarged up to ISO size 40 master apical file with stainless steel K-files using standardized preparation and remaining ten teeth were served as negative control. Then teeth were randomly assigned into different groups depending on sealer used for obturation as follows: Group 1: Negative control-no instrumentation was performed. Group 2: Positive control-gutta-percha with out the use of any sealer. Group 3: Experimental group-gutta-percha and Tubli-Seal EWT root canal sealer. Group 4: Experimental group-gutta-percha and AH Plus. Group 5: Experimental group-epiphany SE sealer and epiphany points. After 72 hours, the specimens were embedded in autopolymerizing resin leaving 7 mm of each root exposed and were subjected to fracture testing under universal testing machine at a crosshead speed of 1.0 mm per minute until the root fractured. Results were statistically analyzed using one-way ANOVA and independent t-test. Showed that Epiphany SE sealer/Epiphany points showed highest mean fracture resistance and Tubli-Seal EWT group showed the least fracture resistance of all the materials tested. There was no statistically significant difference among experimental groups. Epiphany SE sealer/Epiphany points demonstrated highest fracture resistance values than the other materials tested and intact tooth had highest resistance against vertical root fracture. Epiphany SE sealer/Epiphany points may be one of the materials of choice in the endodontic treatment of teeth.

Evaluation of the cytotoxicity of different root canal sealers on L929 cell line by MTT assay

The purpose of this study was to evaluate the cytotoxic effects of commercially available root canal sealers [Sealite Ultra (SU), Tubli-Seal (TS), Tubli-Seal EWT (TS-EWT), Pulp Canal Sealer (PCS), Pulp Canal Sealer EWT (PCS-EWT), Endomethasone N (En N), and Apexit Plus (AP)] on L929 cells by using MTT assay. After incubation with each sealer's extract at 37°C in a humidified air atmosphere containing 5% CO(2 )for 24 h, MTT (5 mg/mL) in saline was added into each well and further incubated at 37°C for 4 h. Formazan precipitate was dissolved in a buffer containing 23% sodium dodecyl sulfate and 50% N, N-dimethylformamide (pH 4.7). Optical densities of dissolved formazan were read using a microplate spectrophotometer. AP, TS, and TS-EWT showed no cytotoxicity at any dilution tested. Other sealers exhibited some degree of cytotoxicity at the 1/4 and 1/2 dilutions. PCS-EWT and SU exerted more potent cytotoxicity at 1/2 dilution than the other sealers.

“Evaluation of quality of apical seal in Resilon/Epiphany SE, gutta-percha/AH Plus and gutta-percha / tubli-seal EWT - filled by dye leakage approach - An in vitro study”

The purpose of this study is to evaluate the apical sealing ability of resilon and epiphany SE root canal sealer when compared with gutta-percha/AH Plus sealer and gutta-percha/tubli-seal EWT sealer using cold lateral condensation obturation technique using dye leakage approach. Sixty four human maxillary permanent central incisors were used with uniform root segment of 16mm. Samples were randomly divided into three experimental groups of 20 teeth each obturated by different combination of obturating materials and four teeth were prepared as controls. Teeth were obturated in group I-with resilon/epiphany SE sealer; in group II-with gutta-percha/AH plus sealer and in group III-with gutta-percha/tubli-seal EWT sealer. Stereomicroscopic evaluation showed mean value dye penetration for group I-2.350 ± 1.148mm, for group II-2.850 ± 0.961mm, and for group III- 4.150 ± 1.226mm. Resilon/Epiphany SE group had the least penetrtion followed by gutta-percha/AH Plus and maximum in guttapercha/ tubli-seal EWT

Evaluation of cytotoxicity of different root canal sealers and their effect on cytokine production.

Endodontic sealers are in direct contact with periradicular tissues and play a critical role in regeneration and pathogenesis of periradicular diseases. The aim of this study was to compare cytotoxicity of four different types of sealers including AH plus, Sankin, Tubliseal EWT and Apexit as well as their effect on cytokine release of L929 fibroblasts. In this experimental study, cells were cultured in Complete Medium Culture (CMC) and then divided into two test groups. In group 1, sealers were added to cell culture wells immediately after mixing. In group 2, sealers were added to cell cultures 3 hours after mixing. Cell viability was evaluated by MTT assay after 4, 24 and 168 hours. The amount of Interleukin-6 (IL-6) released in response to the sealers was also evaluated by ELISA technique on fibroblasts after 24 hour period. Data were analyzed using Kruskal-Wallis and Mann Whitey-U tests. Significant differences were seen in cytotoxicity in both groups (P<0.001). The least cytotoxic sealers were AH Plus and Sankin respectively, whereas Tubliseal EWT showed the greatest cytotoxicity. Production of IL-6 was significantly different among studied groups (P<0.001). The highest IL-6 level was observed in Tubliseal EWT and Sankin groups; which was statistically significant (P<0.001). AH plus has less cytotoxicity and induces less IL-6 release. Tubliseal EWT has greater cytotoxicity and induces more IL-6 release than other sealers. This should be considered during their routine use in root canal treatments.

The effect of temperature on viscosity of root canal sealers

To test the hypothesis that there was no significant (alpha = 0.05) change in viscosity of commercially available root canal sealers with increase in temperature using a high-performance Advanced Rheometric Expansion System (ARES) rheometer. Materials tested were Apexit, Tubliseal EWT, Grossman's, AH Plus and Ketac-endo. Cone-and-plate geometry was used (25-mm diameter, 0.1 radian and gap 0.051 mm). Measurements were carried out for steady-state viscosity at 25 and 37 degrees C in the shear rate range of 0.001-50 s(-1) at standardized relative humidity and within 30 min from the start of mixing. Five samples were taken for each sealer at each temperature. At 25 degrees C all sealers demonstrated shear thinning. At 37 degrees C Grossman's (powder : liquid ratio 2 : 1 and 3 : 1) and Ketac-endo had a rapid rise in viscosity and early set whereas the other sealers were shear thinning. On increasing temperature from 25 degrees C to 37 degrees C, Apexit, Tubliseal and AH Plus had reduced viscosity whereas Grossman's 2 : 1, Grossman's 3 : 1 and Ketac-endo had increased viscosity, which varied with the shear rate. The change in viscosity with change in temperature was significant (P < 0.05) for all sealers except AH Plus. There was a variation in the effect of increasing temperature on each sealer depending on the shear rate. With the exception of AH Plus, a significant (P < 0.05) change in viscosity was found, and the null hypothesis was rejected.

Cytotoxicity and genotoxicity of newly developed calcium phosphate-based root canal sealers

The purpose of this study was to compare the cytotoxicity by MTT test and genotoxicity by Ames test of new calcium phosphate-based root canal sealers (CAPSEAL I, CAPSEAL II) with commercially available resin-based sealers (AH 26, AH Plus), zinc oxide eugenol-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT), calcium hydroxide-based sealer (Sealapex), and tricalcium phosphate based sealers (Sankin Apatite Root Canal Sealer I, II, III). According to this study, the results were as follows: 1. The extracts of freshly mixed group showed higher toxicity than those of 24 h set group in MTT assay (p < 0.001). 2. CAPSEAL I and CAPSEAL II were less cytotoxic than AH 26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, Sealapex and SARCS II in freshly mixed group (p < 0.01). 3. AH 26 in freshly mixed group showed mutagenicity to TA98 and TA100 with and without S9 mix and AH Plus extracts also were mutagenic to TA100 with and without S9 mix. 4. Tubliseal EWT, Pulp Canal Sealer EWT and Sealapex in freshly mixed group were mutagenic to TA100 with S9 mix. 5. Among those of 24 h set groups, the extracts of SARCS II were mutagenic to TA98 with and without S9 mix and AH 26 showed mutagenic effects to TA98 with S9 mix. 6. No mutagenic effect of CAPSEAL I and CAPSEAL II was detected. 7. There is no statistically significant difference between CAPSEAL I and CAPSEAL II at MTT assay and Ames test in both freshly mixed group and 24 h set group.

A study of the rheological properties of endodontic sealers

To test the hypothesis that there would be no statistically significant difference in viscosity-related measures of endodontic sealers or change in these with strain rate, internal diameter or powder : liquid ratio in a capillary system. Materials used were Apexit, Tubliseal EWT, Grossman's sealer and Ketac-endo. Viscosity-related measures were tested in a two-plate test, and in a capillary rheometer. The mean values (n = 12) for thickness and diameter of material formed between two glass plates were tested with one-way analysis of variance. Pressure was applied to a capillary rheometer at strain rates 5 and 10 mm min(-1) in tubes of internal diameter 0.6 and 1.2 mm. Tubliseal EWT had a thinner film thickness than the other sealers (alpha = 0.05). The difference in diameter between Tubliseal EWT and the other sealers was significant apart from Apexit. Increased strain rate gave a significant increase (alpha = 0.05) in the flow of all sealers. Narrower tubes produced increased velocity, which was significant for all sealers, and reduced volumetric flow, which was significant for all sealers except Grossman's 2 : 1 (Wilcoxon signed rank test). Reduction in powder : liquid ratio of Grossman's significantly increased flow in narrow tubes and at higher strain rate (Mann-Whitney test). There was a significant difference between the flow of Tubliseal EWT and the other sealers tested in the two-plate test; capillary flow was affected by sealer, internal diameter, strain rate and powder : liquid ratio. The null hypotheses were rejected.

Cytotoxicity of resin-based root canal sealer, adseal

The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex). An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at 37℃ in humidified 5% CO 2-containing air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 ㎜ and height 5 ㎜ placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1, 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex

Polymicrobial coronal leakage of super EBA root-end fillings following two methods of root-end preparation

The aim of this study was to compose in vitro coronal leakage of a super EBA root-end filling material after two root-end cavity preparation techniques. A mixed anaerobic microbial marker was used. Forty-five extracted human teeth with straight, single root canals were prepared chemo-mechanically to a size 40 master apical file. The teeth were divided into experimental groups (35 teeth) and control groups (10 teeth). Forty teeth (35 experimental teeth and five negative control teeth) were obturated by lateral condensation of cold gutta-percha with Tubliseal EWT sealer. The remaining five teeth were not obturated and served as positive controls. These teeth were stored for 6 months in artificial saliva. The apical 3-4 mm of each root was resected perpendicular to the long axis of the root and a root-end cavity prepared to a depth of 3 mm using either a size 008 rosehead burr or an ultrasonic retroprep tip. Freshly mixed EBA cement was placed into the root-end cavity. The entire root surface of each tooth, except the cutting surface of the apical end, was sealed with nail varnish. The coronal part of each root canal was sealed with the cut end of a tube and placed in a bottle containing sterile Brain Heart Infusion Broth (BHIB). A marker consisting of Anaerobic streptococci and Fusobacterium nucleatum in BHIB was placed in each coronal chamber at 7-day intervals and daily observations were made for bacterial growth in the apical chamber for 60 days. All positive control teeth exhibited bacterial leakage within 48 h, whilst the apical chamber of negative control teeth remained uncontaminated throughout the test period. Fifty-nine percent (n= 10) of the specimens prepared with a burr showed leakage after 90 days, whilst only 22% (n= 4) of the ultrasonically prepared group showed leakage after the same time. The group prepared with ultrasonic tips showed statistically significant less specimens with leakage (P < 0.05) than the group prepared with burrs.

Coronal leakage of obturated root canals after long-term storage using a polymicrobial marker

This in vitro study investigated the effect of long-term storage on the coronal leakage of a microbial marker on teeth root filled with lateral condensation of cold gutta-percha and one of two sealers. Sixty single-rooted teeth were prepared chemomechanically to a size 40 master apical file. The teeth were divided into two groups of 20 teeth each and obturated with gutta-percha using either Apexit or Tubliseal EWT sealer. The teeth were stored for 6 months in artificial saliva and tested for leakage using a marker consisting of S. sanguis and P. intermedia. The teeth were checked for bacterial leakage daily for 90 days. All positive control teeth leaked after 24 h, while the negative control teeth remained uncontaminated throughout the test period. Leakage reached the apex through the experimental teeth at the earliest at 17 days and at latest at 88 days. Fifty percent and 70% of the specimens of the Apexit and Tubliseal EWT groups respectively showed leakage at 90 days. There was no statistically significant difference (p > 0.05) between the two groups.