In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus.

Duddi Narendra Nirupama,Hulimangala Hosakote Lingareddy Usha,Roshni Sharma,Mohan Thomas Nainan,Sethumadhavan Muralidharan,Rajendran Ramaswamy,Soham Gupta

doi:10.1155/2014/383756

Duddi Narendra Nirupama, Hulimangala Hosakote Lingareddy Usha + Show 5 more

Open Access

https://doi.org/10.1155/2014/383756

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Abstract

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms, E. faecalis, C. albicans, and S. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity against E. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity against C. albicans and S. aureus compared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity against E. faecalis, C. albicans, and S. aureus.

Highlights

Microbes and microbial products are the main etiologic factors of pulpitis and apical periodontitis
The results of Agar diffusion test (ADT) are highly influenced by solubility and diffusibility of the test agent through the agar, and, this test is not suitable to assay water insoluble materials [13]. This could be overcome by direct contact test (DCT) which was used in this study and was introduced by Weiss et al It is a turbidometric method which detects the bactericidal and bacteriostatic effect of endodontic sealers and root end filling materials
Of the four sealers tested, significant antimicrobial activity was observed for AH Plus and iRoot SP against the positive control (P < 0.05)

Summary

Introduction

Microbes and microbial products are the main etiologic factors of pulpitis and apical periodontitis. The results of ADT are highly influenced by solubility and diffusibility of the test agent through the agar, and, this test is not suitable to assay water insoluble materials [13] This could be overcome by direct contact test (DCT) which was used in this study and was introduced by Weiss et al It is a turbidometric method which detects the bactericidal and bacteriostatic effect of endodontic sealers and root end filling materials. In the present study we evaluated the antimicrobial activity of four root canal sealers: an epoxy resin-based sealer, AH Plus (Dentsply International Inc., York, PA); zinc oxide-eugenol based sealer, Tubliseal EWT (Sybron Endo Corporation, Orange, CA); polymethacrylate resin-based sealer, EndoRez (Ultradent, South Jordan, UT); calcium hydroxide-calcium silicate complex sealer, iRoot SP (Innovative Bioceramix, Vancouver, Canada) against E. faecalis, C. albicans, and S. aureus by DCT. By using DCT was the direct antimicrobial activity of the tested endodontic sealers assessed, and components of endodontic sealers capable of diffusing into the medium were assessed and statistically compared

Materials and Methods

Results

Discussion