Tuberous Roots Research Articles

Purple sweet potato polysaccharide ameliorates concanavalin A-induced hepatic injury by inhibiting inflammation and oxidative stress

BackgroundAutoimmune hepatitis (AIH) is a prevalent liver disease that can potentially lead to hepatic fibrosis and cirrhosis. The prolonged administration of immunosuppressive medications carries significant risks for patients. Purple sweet potato polysaccharide (PSPP), a macromolecule stored in root tubers, exhibits anti-inflammatory, antioxidant, immune-enhancing, and intestinal flora-regulating properties. Nevertheless, investigation into the role and potential mechanisms of PSPP in AIH remains notably scarce. PurposeOur aim was to explore the possible protective impacts of PSPP against concanavalin A (Con A)-induced liver injury in mice. MethodsPolysaccharide was isolated from purple sweet potato tubers using water extraction and alcohol precipitation, followed by purification through DEAE-52 cellulose column chromatography and Sephadex G-100 column chromatography. A highly purified component was obtained, and its monosaccharide composition was characterized by high performance liquid chromatography (HPLC). Mouse and cellular models induced by Con A were set up to investigate the impacts of PSPP on hepatic histopathology, apoptosis, as well as inflammation- and oxidative stress-related proteins in response to PSPP treatment. ResultsThe administration of PSPP significantly reduced hepatic pathological damage, suppressed elevation of ALT and AST levels, and attenuated hepatic apoptosis in Con A-exposed mice. PSPP was found to mitigate Con A-induced inflammation by suppressing the TLR4-P2X7R/NLRP3 signaling pathway in mice. Furthermore, PSPP alleviated Con A-induced oxidative stress by activating the PI3K/AKT/mTOR signaling pathway in mice. Additionally, PSPP demonstrated the ability to reduce inflammation and oxidative stress in RAW264.7 cells induced by Con A in vitro. ConclusionPSPP has the potential to ameliorate hepatic inflammation via the TLR4-P2X7R/NLRP3 pathway and inhibit hepatic oxidative stress through the PI3K/AKT/mTOR pathway during the progression of Con A-induced hepatic injury. The results of this study have unveiled the potential hepatoprotective properties of purple sweet potato and its medicinal value for humans. Moreover, this study serves as a valuable reference, highlighting the potential of PSPP-1 as a drug candidate for the treatment of immune liver injury.

Structural, Thermal, Pasting and Digestion Properties of Starches from Developing Root Tubers of Sweet Potato.

Three sweet potato varieties with white-, yellow- and purple-fleshed root tubers were harvested at 100, 120, 140 and 160 days after planting (DAP). Their starch structural, thermal, pasting and digestion properties were measured to reveal the influences of harvesting dates on the physicochemical properties of sweet potato root tuber starch. Though starches from different varieties displayed some differences in physicochemical properties due to their different genetic backgrounds, they were influenced by harvesting date in similar ways. Starches isolated from root tubers at 100 and 160 DAP exhibited lower granule sizes than those at 120 and 140 DAP. The amylose content was higher in root tubers at 100 and 120 DAP than at 140 and 160 DAP. Starches from root tubers at 100 DAP exhibited CA-type X-ray diffraction patterns, and then the B-type crystallinity gradually increased at later harvesting dates. The different harvesting dates had no significant effects on the short-ranged ordered structure and lamellar thickness of starch, but the lamellar peak intensity decreased significantly at later harvesting dates. Starch had a lower gelatinization temperature and a wider gelatinization temperature range in root tubers at 140 and 160 DAP than at 100 and 120 DAP. The higher peak viscosity and lower pasting temperature were associated with the late harvesting date. The digestion of starch had slight differences among root tubers at different harvesting dates. The harvesting dates of root tubers played more important roles in starch properties than the variety. This study would be helpful for breeders, farmers and sweet potato starch users.

Improving the yield and nutritional quality of cassava (Manihot esculenta Crantz) in the rainforest agro-ecological zone of southeast Nigeria through agronomic biofortification with micronutrients

Cassava (Manihot esculenta Crantz) is an important staple crop, especially for resource-poor populations. Cassava-based diets are rich in calories but grossly deficient in essential mineral elements. A field study in southeastern Nigeria aimed at increasing yields and concentrations of bioavailable mineral elements in a β-carotene cassava variety, UMUCASS 38 (TMS 01/1371), through agronomic biofortification. A randomized complete block design experiment in triplicate, with foliar application of Zn (3.0 kg ha−1 Zn as ZnSO4), I (4.0 kg ha−1 I as KI), Se (0.25 kg ha−1 Se as Na2SeO3) and Zn + Se + I in combination at vegetative, tuber initiation and bulking stages of cassava. Results indicated neither phytotoxicity nor altered physical and chemical soil properties. Application of Zn or Zn + Se + I at the vegetative stage significantly increased cassava tuber yields. More Zn, Se, and I accumulated in fresh and processed cassava tuber products (gari, lafun), especially when Zn + Se + I was applied at tuber initiation stage. The combined application of Zn + Se + I consistently outperformed sole applications indicating a synergistic interaction effect between the three elements. It is concluded that in the Zn-deficient soils of southeast Nigeria, yield of cassava root tubers can be significantly increased by foliar application of Zn-containing fertilizer at the vegetative stage, but for optimal accumulation of elemental Zn, Se, and I, a combined Zn + Se + I fertilizer be applied at the tuber initiation stage.

Enhancing the nutritional quality of fufu with a starter culture

This study investigates the nutritional enrichment of fufu, a staple African food, by controlling the fermentation of cassava root tuber using a starter culture. Lactic acid bacteria (LAB) were isolated from fermented cassava and analyzed for their technological properties. The physicochemical parameters, proximate and antinutrient content of the fufu samples were determined by standard analytical methods. Twelve LAB were identified as Lactobacilli plantarum (42%), L. acidophilus (25%), L. fermentum (17%), L. brevis (8%), and L. mesenteroides (8%). The LAB isolates produced lactic acid, diacetyl, and hydrogen peroxide ranging from 1.90-2.90, 1.30-2.10, and 1.10 -2.90 mg/mL respectively. Lactobacillus plantarum (FF8) was selected as a starter culture due to its exceptional ability to produce antimicrobial substances, leading to higher yields of lactic acid, diacetyl, and hydrogen peroxide, reducing the fermenting medium's pH. The pH changes in starter-induced fermented fufu (SIFF) and spontaneous fermented fufu (SFF) samples from 0 to 96 hours were 7.10 - 2.60 and 7.10 - 3.30, respectively, while the Total Titratable Acidity (TTA) increased from 0.71-1.79 and 0.28-0.51, respectively. Starter-induced fermented fufu (SIFF) has higher protein, fat, sodium, potassium, iron, zinc, phosphorus, and Vit. C, B1, and A content of 2.93, 0.23 (%) 596.4, 270.9, 8.93, 1.67, 296.67, 5.28, 0.24, and 0.31 (mg/100g) respectively, compared to spontaneous fermented fufu and a significant decrease in antinutrient content, such as cyanide, saponin, and phytates of 0.05, 0.16, and 0.06 (mg/100g), respectively. The study found that L. plantarum FF8 used as a starter culture, improves the nutritional value of fufu and reduces anti-nutrients, suggesting potential health benefits for consumers.

Effects of Farming Conditions on the Nutritional Contents of Manihot esculenta Planted in four Regions of Niger Delta, Nigeria

All through human existence, the ability to cultivate crops to better human life has been affected by the nature of the soil; different crops strive in different regions. The relationship between humans, the earth and food production affirms soil as the foundation of crop production. Hence, the objective of this paper as to the effects of farming conditions on the nutritional contents of Manihot esculenta planted in four regions of Niger Delta, Nigeria. Using appropriate standard methods. Data obtained revealed that the moisture contents varied in the order; herbicide > oil-spilled > inorganic fertilizer > normal. The amount of water-soluble vitamins varied in the order; normal > oil spilled > inorganic fertilizer > herbicide. The amount of crude lipids varied in the order; herbicide > normal > inorganic fertilizer > oil-spilled. The amount of hydrogen cyanide varied in the order; inorganic fertilizer > herbicide > oil-spilled > normal. The amount of carbohydrate varied in the order; inorganic fertilizer > oil-spilled > normal > herbicide. The amount of crude protein and fiber varied in the order; herbicide > normal > inorganic fertilizer > oil-spilled. Root tubers from the normal practice was most abundant in ash contents, while that from the use of herbicide was the least. These results indicated that the normal practice showed commended amount of moisture content, water soluble vitamins and ash content; the herbicide induced land produced cassava tuber with the highest amount of crude lipids, crude fibre and crude proteins; that with the use of inorganic fertilizer produced the highest amount of hydrogen cyanide. All practices were adoptive for carbohydrate formation, except the herbicide induced soil.

SNP Markers Associated with Some Root, Stolon, and Tuber Traits in Tetraploid Potatoes (Solanum tuberosum L.) Grown Under Diverse Growing Systems

AbstractNumerous loci, environmental factors, and their interactions have an impact on the phenotypic diversity of several significant traits in plants. One approach put forth in recent years for genetic research and finding quantitative trait loci (QTLs) responsible for the specific trait is association mapping. The purpose of the current study was to pinpoint the genetic underpins of significant underground traits in potato. A panel of 192 diverse tetraploid potato genotypes from different countries were grown under different growing conditions (i.e., aeroponics and pot) to study root, stolon and tuber traits. Significant differences (P ≤ 0.01) were found between the genotypes for all examined traits, and the heritability (H2) of the traits ranged from 0.74 to 0.94. Genotyping was carried out using the SolCAP 25K array. 21,226 polymorphic SNPs were used for association mapping of underground traits. A GWASpoly R package was implemented for the marker-trait associations, and 78 genomic regions were found associated with the traits under investigation.. The history of potato breeding was reflected in LD patterns. The identified SNPs have their putative gene functions related to the root and stolon architecture and tuber growth (i.e., WRKY transcription factor, MAPK, the GTP cyclohydrolase 1 (i.e., GTPCHI), Glutathionyl-hydroquinone reductase, and pyrophosphate—fructose 6-phosphate 1-phosphotransferase subunit alpha (PFPase). The results of the present study provides a framework that could be helpful for future potato breeding programs to increase tuber production and reduce the challenges of feeding the world's population in the years to come.

Genome-Wide Identification and Expression Analysis of the Starch Synthase Gene Family in Sweet Potato and Two of Its Closely Related Species.

The starch synthase (SS) plays important roles in regulating plant growth and development and responding to adversity stresses. Although the SS family has been studied in many crops, it has not been fully identified in sweet potato and its two related species. In the present study, eight SSs were identified from Ipomoea batatas (I. batata), Ipomoea trifida (I. trifida), and Ipomoea trlioba (I. trlioba), respectively. According to the phylogenetic relationships, they were divided into five subgroups. The protein properties, chromosomal location, phylogenetic relationships, gene structure, cis-elements in the promoter, and interaction network of these proteins were also analyzed; stress expression patterns were systematically analyzed; and real-time polymerase chain reaction (qRT-PCR) analysis was performed. Ipomoea batatas starch synthase (IbSSs) were highly expressed in tuber roots, especially Ipomoea batatas starch synthase 1 (IbSS1) and Ipomoea batatas starch synthase 6 (IbSS6), which may play an important role in root development and starch biosynthesis. At the same time, the SS genes respond to potassium deficiency, hormones, cold, heat, salt, and drought stress. This study offers fresh perspectives for enhancing knowledge about the roles of SSs and potential genes to enhance productivity, starch levels, and resistance to environmental stresses in sweet potatoes.

Review on the Therapeutic Role of Asclepias tuberosa Seeds in Diabetes Management

Diabetes mellitus (DM) presents a significant global health challenge characterized by elevated blood glucose levels, leading to debilitating complications. Despite various therapeutic approaches, effective glucose regulation remains elusive, often accompanied by adverse effects from current medications. Medicinal plants, including Asclepias tuberosa, have shown promise in managing blood glucose levels with fewer side effects. This review highlights the potential of Asclepias tuberosa as herbal antidiabetic agents based on recent literature, suggesting avenues for further research and therapeutic development in DM management. Considering the urgent need for safer and more effective antidiabetic treatments, exploring the therapeutic potential of Asclepias tuberosa presents a promising avenue for addressing this growing public health concern. Future studies should focus on elucidating the mechanisms of action, conducting clinical trials to validate the efficacy and safety of Centaurea-based interventions in DM management, and exploring synergistic effects with existing therapies.
 Furthermore, within the context of utilizing plant materials for the treatment of Diabetes mellitus, particular attention is directed towards the utilization of Asclepias tuberosa seeds. Asclepias tuberosa, commonly known as butterfly weed or pleurisy root, is a medicinal plant native to North America. Traditionally, various parts of this plant, including the roots, leaves, and seeds, have been used in folk medicine for their purported therapeutic effects
 Keywords: Diabetes mellitus, Asclepias tuberosa, antidiabetic agents, herbal remedies, blood glucose regulation.

Assessment of heavy metals and human health risk associated with the consumption of crops cultivated in industrial areas of Maputo, Mozambique

This study aimed to evaluate heavy metals concentrations in soils and vegetables (cabbage, lettuce, and cassava) cultivated at Matola and Beluluane Industrial Parks, and to assess health risks linked to their consumption through estimated daily intake, hazard index (HI), and incremental lifetime cancer risk. Concentrations of Al, As, Co, Cd, Cr, Ni, Pb, and Zn were determined in the two sites. Soil concentrations of As at Beluluane site and As, Cd, and Cr at Matola site exceeded reference limits of the Food and Agriculture Organization/World Health Organization, showing heavy metal contamination. At Beluluane site, all studied vegetables presented As and Pb levels higher than reference limits, Cd concentrations were higher than the reference limit in cabbage, lettuce, and cassava leaves. At Matola site crops concentrations of As, Cd, Cr, and Pb exceeded the reference limits. Zinc exceeded the reference limit in all crops except in cabbage. HIs for vegetables from Beluluane exceeded 1.0 in cabbage (2.66), lettuce (2.27), and cassava leaves (2.37). Likewise, at Matola, HIs exceeded 1.0 in lettuce (1.67), cassava leaves (1.65), and root tubers (13). We found that vegetables cultivated in industrial parks present high carcinogenic risk due to heavy metal contamination, rendering them unsuitable for human consumption.

An Overview of Approaches of Cassava Processing and Cassava Based Recipe Preparation in Ethiopia

Cassava (Manihot esculenta Crantz) is tuberous roots, drought tolerant, which is high in carbohydrate content and a cheap source of food. However it needs removal of toxic substance which is called hydrogen cyanide (HCN) which can be easily removed. There are two types of common preparation methods, preparing it by drying it in the sun and preparing it by boiling. Removing toxic cyanide by drying is less effective, however boiling is better method of removing cyanide from cassava roots. Cassava is used as a raw material in different food industries. In Ethiopia, there is cassava processing industry around Addis Ababa located at Dukem area. There are different trends of cassava based recipe preparations. Especially in Ethiopia there are exotic cassava based recipe items. Some of the trends in Ethiopia as formulated by Jimma Agricultural Research Center are; cassava bread with different flour compositions, cassava with corn flour composite recipe, cassava with teff flour composite recipe, Cassava syrup stew, Cassava porridge, Cassava biscuits and so on are exotic recipe with good sensorial values and proximate compositions. Due to its drought tolerant nature and nutritional excellence; cassava production, utilization and popularization is best strategy for food system resilience in the world wide.

Molecular Genetic Diversity of Cassava (Manihot esculenta CRANTZ) in the Maritime Region of Togo using Simple Sequence Repeat (SSR) Markers

Cassava is a plant cultivated for its calorie-rich leaves and tuberous roots. Unfortunately, genetic improvement of cassava is limited by the lack of information on its genetic variability. The objective of the present work was to study the genetic and molecular diversity of cassava cultivars using microsatellite markers (SSR) in the Maritime region of Togo. A total of 95 cassava individuals from 7 cultivars were evaluated using 7 SSRs. The average polymorphic information content was 0.74 and the average number of alleles per locus was 7. The average observed heterozygosity was lower than the expected average heterozygosity for the 7 cultivars, showing positive fixation indices and presence of inbreeding. Molecular analysis of variance revealed greater molecular variation within cultivars (94%). The dendrogram constructed using the Neighbor Joining method resulted in 3 groups. The high genetic diversity observed could be due to the exchange of cuttings by growers. This diversity would be valuable for efficient germplasm management and for effective use of the material in breeding.

Effect of axillary bud number on stem cuttings of sweet potato cv. 'Blesbok' productivity using sack gardening technology

Increasing urbanisation and the rise in global food prices, which results in severe hunger and micronutrient deficiencies, necessitates the identification of innovative, but affordable vegetable growing-based strategies, that can increase access to food by utilising the limited space available in rural and urban areas. Therefore, sack gardening technology could be a solution to both the lack of arable land and water scarcity in urban and rural areas. The objective of the study was to determine whether axillary bud number will have an effect on the agronomic parameters of sweet potato cv. 'Blesbok' produced using the sack gardening technology. The number of axillary buds per stem cutting, namely, 1 (control), 2, 3, 4, and 5 buds, were arranged in RCBD, with 10 replicates. The number of axillary buds had significant effects on vine length (VL), fresh shoot mass (FSM), number of shoots (NOS) and dry shoot mass (DSM). However, no significant effects were observed on fresh tuberous root mass (FTRM), chlorophyll content (CC), stem diameter (ST), tuberous root diameter (TRL), tuberous root length (TRL), dry tuberous root mass (DTRM) and number of tuberous roots (NTR). Relative to the control VL, FSM and DRM was reduced by 8-37, 30-55 and 26-55%, in stem cuttings having 2, 3, 4 and 5 buds, respectively, whereas the NS was increased by 8-17%. The quadratic relationship models were explained by 95, 92, 96 and 98% in VL, NOS, FSM and DSM, respectively. In conclusion, stem cuttings containing 1 and 2 axillary buds showed better growth parameters when grown under sack gardening technology.

Multi-omics profiling reveals an extensive regulation network shift in the domestication process of the traditional Chinese medicinal herb Rehmannia glutinosa Liboschitz (Orobanchaceae)

Plant domestication plays a vital role in human evolution and society development. Contrasted to the major crops, the domesticated procedure of medicinal herbs remains largely unknown. Here we analyzed the epigenomic, transcriptomic, and metabolomic profiling of three wild and nine cultivated samples of Dihuang (Rehmannia glutinosa Liboschitz), a well-known traditional Chinese medicinal herb, to elucidate the genetic differentiation and regulation mechanism during domestication. The results unraveled that tremendous differentiation existed among wild and cultivated types from genomes to metabolites. The cultivars presented significantly more methylated sites than the wild type and conjoint analyses indicated that hypermethylation of key genes was generally related to their down-regulated expressions and up-regulated metabolites in the pathways of monoterpenoid biosynthesis and carbohydrate metabolism involved in main components production and tuberous root enlargement. We also identified the key gene related to plant circadian rhythms and potential adaptation in the domestication of Dihuang. This work unveiled the systematic transformation from wild to cultivated Dihuang and provides new insights into the regulatory networks of genetic architectures, benefiting germplasm collection, cultivation, and utilization in the future.

Fertirrigação com cobre na cultura da beterraba em região semiárida

ABSTRACT Copper is an essential element for beet; however, it needs to be made available in adequate quantities since this nutrient, which is a heavy metal, can cause toxicity to plants and/or humans. This study aimed to evaluate the agronomic performance of beet fertigated with copper in a semi-arid region of Brazil. Two experiments were carried out in complete randomized blocks, with five treatments (0, 1.5, 3.0, 4.0, and 6.0 kg ha-1 of copper) and four replicates. In 2019, the copper content was within the adequate range for beet. In 2021, fertigation with copper above 0.8 kg ha-1 led to contents in the phytotoxicity range, which may explain the reduction in total and tuberous root dry mass accumulation. Fertilization with copper increased marketable yield in 2019 (17.32 t ha-1 at the dose of 3.9 kg ha-1 of copper), while, in 2021, there was no fit of the equations. Copper fertilization altered the tuberous root pH but did not influence the other tuberous root quality components. Plants fertilized with copper accumulated more copper in the leaves than in the tuberous root, with a maximum accumulation of 6.0 kg ha-1 of copper in 2019 and 2021. Fertilization with 3.9 kg ha-1 suits soils with low copper content.

Relictithismia kimotsukiensis, a new genus and species of Thismiaceae from southern Japan with discussions on its phylogenetic relationship.

The family Thismiaceae, known as "fairy lanterns" for their urn- or bell-shaped flowers with basally fused tepals, consists of non-photosynthetic flowering monocots mainly in tropical regions, extending into subtropical and temperate areas. Here, we propose a new mycoheterotrophic genus, Relictithismia Suetsugu & Tagane (Thismiaceae), with its monotypic species Relictithismia kimotsukiensis Suetsugu, Yas.Nakam. & Tagane from Kimotsuki Mountains in the Osumi Peninsula, Kagoshima Prefecture, Kyushu Island, southern Japan. Relictithismia resembles Haplothismia Airy Shaw in having a cluster of tuberous roots, a feature previously observed only in this genus within the family Thismiaceae. However, it differs in having solitary flowers (vs. 2-6-flowered pseudo-raceme in Haplothismia), anther thecae largely separated (vs. connate), and the presence of an annulus (vs. absent). Additionally, Relictithismia differs from the geographically overlapping genus Thismia Griff. in its stamen structure and the position of the annulus. In Relictithismia, the stamens lack connectives, and its free filaments arise from the annulus located inside the perianth mouth, while in Thismia, the stamens typically have connate connectives, forming a staminal tube pendulous from the annulus located at the mouth of the floral tube. Our morphological and phylogenetic data indicated that R. kimotsukiensis holds an early-diverging position within the family, situated outside the Old World Thismia clade. This paper offers an extensive description and color photographs of R. kimotsukiensis, complemented by notes on its phylogenetic relationship and evolutionary history.

Assessment of Yellow and White Fleshed Cassava Tuberous Root Cultivars Reveals Different Responses to Post-harvest Physiological Deterioration

Identification of post-harvest physiological deterioration (PPD) tolerance in cassava is crucial, as PPD significantly hampers the cassava tuberous root industry by shortening storage periods post-harvest and diminishing product quality. Characteristics linked to PPD tolerance encompass high carotenoid levels and low dry matter content (DMC). This study aimed to evaluate the PPD responses of six yellow fleshed and ten white-fleshed cassava tuberous roots and determine the source of PPD tolerance. PPD and DMC assessments were conducted using standard methods at three storage periods: 2, 5, and 10 days after harvest (DAH). The k-means clustering analysis revealed six clusters, each corresponding to distinct PPD symptom patterns and tolerance statuses. Cluster 1, comprising three yellow-fleshed and three white-fleshed cassava cultivars, demonstrated PPD tolerance with minimal symptoms up to 5 DAH. Clusters 2 and 3 exhibited a moderate PPD response with elevated symptoms at 5 DAH, comprising three yellow-fleshed and three white-fleshed cassava cultivars. Clusters 4 to 6 displayed a sensitive response to PPD, showcasing a significant increase in symptoms at 5 and 10 DAH, with four white-fleshed cassava cultivars identified within this cluster. These findings underscored the presence of PPD tolerance in both yellow-fleshed and white-fleshed cassava tuberous roots. The correlation between PPD and DMC was significant only at 2 DAH, displaying a moderate positive correlation. Consequently, this study identified three cultivars, “Carvita-25”, “Manggu”, and ““Ubi Kuning””, with high DMC and low PPD incidence at 5 DAH, suggesting their suitability for further breeding programs.

Influencia de la fertilización inorgánica sobre parametros productivos de dos variedades de camote

In order to determine the effect of inorganic fertilization treatments on the productive behavior of the sweetpotato varieties "INIAP-Toquecita" and "INIAP-Buena Vista", this research was carried out during the dry season (June–December 2022) at the Estación Experimental Portoviejo del Instituto Nacional de Investigaciones Agropecuarias-INIAP, in the Colón parish, canton Portoviejo, province of Manabí, Ecuador. A randomized complete block design was used with three replications, and the following variables were analyzed: percent yield, total number of guides per plant, length of guides, mass of 100 guides, foliage yield, and number and yield of commercial roots. The inorganic fertilization treatments under study were: 1. N+P2O5+K2O+S+Mg+B; 2. N+P2O5+K2O+S+Mg+B+Zn+Mn+Fe; 3. 50 % more N+P2O5+K2O+S+Mg+B with respect to treatment 1; 4. N+P2O5+K2O; and 5. no fertilizers (control). It was evidenced that the inorganic fertilization treatments implemented on the variety "INIAP-Buena Vista" outperformed the results of the control, while treatments 1 and 2 of inorganic fertilization implemented on the variety "INIAP-Toquecita" outperformed the control. It was observed that the most favorable scenario for maximizing the yield of commercial tuberous roots in the variety "INIAP-Buena Vista" was treatment 3, which had 50 % more macro and micronutrients per hectare added to the soil with respect to treatment 1. The varieties evaluated did not show nutritional deficiencies during the production cycle (120 days).

Waterlogging Hardening Effect on Transplant Stress Tolerance in Pinus densiflora

Waterlogging induces oxidative damage by accumulation of reactive oxygen species due to stomatal closure. Plants alter their physiological and molecular mechanisms to reduce and adapt to oxidative stress. This mechanism of adaptation to stress, known as hardening, can support future stress tolerance. Pinus densiflora seedlings were grown under waterlogging treatment for three years and then transplanted to another site to identify the waterlogging hardening effect on transplanting. Transcriptome analysis was conducted before and after transplanting, and physiological factors were measured after transplanting. After transplanting, wounding stress is the main cause of transplant stress, and 13 genes related to phenylpropanoid were upregulated for the recovery of wounded roots in waterlogged hardened seedlings. The leaf starch and soluble sugar content of the waterlogged hardened seedlings were 50.3% and 40.5% lower due to the formation of cell walls. However, auxin-related genes were downregulated in waterlogging hardened seedlings, resulting in a lower tendency for height growth in hardened waterlogged seedlings. Waterlogging hardening mitigated transplant stress by wounding more than non-hardening, whereas waterlogging hardening may negatively affect seedling height. Our study provides evidence for the hardening effect of long-term waterlogging on transplanted P. densiflora seedlings.

Agronomic performance of beet as a function of fertilization with boron

Boron (B) is a micronutrient that plays a role in the growth and development of plants. In beetroot (Beta vulgaris L.), B allows increased yield and accumulation of dry mass. This work aimed to evaluate the agronomic performance of beetroot fertigated with B in the municipality of Mossoró, RN. To this end, two field experiments were conducted in Mossoró, RN, Brazil, designed in complete randomized blocks. Five doses of B (0, 1.0, 2.0, 3.0, and 4.0 kg ha−1 of B) were tested, with four replications. The treatments did not influence the plants’ height, the number of leaves per plant, or the postharvest quality characteristics of the tuberous root. The maximum total and commercial yield of tuberous roots was 22.22 and 21.29 t ha−1, respectively, fertilizing with 4.0 kg ha−1 of B. This dose also promoted the highest accumulation of B in the plant. Using doses above those evaluated can promote greater yield of tuberous beetroot. Therefore, further studies are necessary to determine the recommended dose of B for beets grown in semi-arid regions.

First Report of Apiospora arundinis Causing Wet Root Rot of Pseudostellaria heterophylla in China.

Pseudostellaria heterophylla is one of the traditional medicines in China. From 2020 to 2022, postharvest wet root rot disease was observed with an incidence of 2~5% on the tuberous roots of the harvested P. heterophylla in Zherong county, Fujian province, China, which usually occurs under damp and unventilated conditions. The symptoms of the disease were as follows: white mycelia grew on the surface of tuberous root initially and gradually wrapped around the roots, the internal root tissue turned yellow and became wet decay finally. To identify the causal agent, a total of 20 samples with symptomatic tuberous roots were collected. Small pieces (3 mm2) were treated by surface disinfection with 75% ethanol and 1% NaOCl, then rinsed 3 times with sterile water. These treated pieces were transferred onto potato dextrose agar (PDA) and incubated at 25°C in the dark for 7 d. Ten pure cultures were obtained using single-spore isolation method. The fungus colonies initially produced white aerial mycelium, subsequently exhibited yellow pigmentation. Mycelia were consisted of smooth, hyaline, branched, and septate hyphae. The conidia were solitary or clustered, brown or dark brown, smooth, ellipsoidal to spherical, 6.66 (5.50-7.81)×5.65 (4.17-7.22) µm (n=50) in size. The conidiophores were hyaline or pale brown and produced conidiogenous cells, which were pale brown, smooth, ampulliform, and 10.14 (8.82-15.30) um long (n=50). Based on these morphological characteristics, the fungus was identified as the genus Apiospora (Arthrinium). The rDNA-ITS region and partial β-tubulin gene (BenA) were amplified using the primers ITS1/ITS4 (White et al. 1990) and Bt2a/Bt2b (Glass and Donaldson 1995), respectively. The sequences of isolates FJAT-32563 and FJAT-32564 were deposited in GenBank (ITS, OM920984 and OM920985; BenA, OM953823 and OM953824). All sequences had more than 99% similarity with those of A. arundinis strain CBS:106.12 (ITS, KF144883; BenA, KF144973). In the multilocus phylogenetic analysis (ITS + BenA), the two isolates clustered together with other strains of A. arundinis with 100% bootstrap support. The isolates were therefore identified as A. arundinis based on both morphological and molecular characteristics. To confirm the pathogenicity, fresh tuberous roots were selected and surface disinfected, then the roots were immersed with a quarter length in the conidial suspension (106/mL) for 30 min, whereas the control roots were immersed with sterile water (n=30). They were placed in petri dish with wet filter paper at 25±2℃, maintaining 80% relative humidity in the dark. The white aerial mycelium appeared at 5 days after inoculation, and wet root rot decaying occurred after inoculation for 21 days. The symptoms were similar to those described above, whereas the control roots were asymptomatic. The same fungus was re-isolated from the infected roots, showing similar morphological characteristics and molecular traits. Koch's postulates were completed and the pathogenicity test for the isolates has been repeated thrice. Previously, A. arundinis was reported to infect peach and sugarcane (Ji et al. 2020; Liao et al. 2022). To our knowledge, this is the first report of A. arundinis causing wet root rot of P. heterophylla in China. The disease would be a potentially new threat to this medicinal plant.