Truss Network Research Articles

Effect of formalin preservation on studies on morphology and condition factor of the freshwater shark Wallago attu (Bloch and Schneider, 1801) using truss network system

The study was aimed to evaluate the effect of formalin preservatiion on the shape, size and condition factor of the freshwater silurid catfish Wallago attu (Bloch and Schneider, 1801) using length-weight relationship (LWR), condition factor and truss analysis. A total of 50 specimens of the fish were procured from the river Ganga at Kanpur region. The fresh and formalin preserved fish were measured for total length, standard length and body weight. Results of LWR revealed that the fresh and preserved fish followed different growth pattern and cube law. High values of r2 were obtained for the relationship between length and weight of the fish before and after preservation of the fish in formalin. Decrease in length and an increase in weight was observed in the preserved specimens of the fish due to the effect of formalin but were not statistically significant. The mean condition factor (K) showed significant (p<0.05) change in the fish after preservation. The univariate and multivariate analysis of truss measurements, two partially overlapping different convex hulls of the scatter plot of PC1 on PC2 and canonical discriminant functions showed highly significant morphometric heterogeneity between the specimens of fresh and preserved fish. The results of the present study suggest that fresh specimens of the fish should be used in research studies based on weight and linear body measurements of the fish. Keywords: Condition Factor, Formalin, Length-weight relationship, Truss network system

Genetic and morphological impact of the cultured gilthead sea bream (Sparus aurata Linnaeus, 1758) populations on wild stocks

Sparus aurata is a very important fish, in fisheries and aquaculture. In this study, the genetic impact of cultured gilthead sea bream S. aurata was examined on wild stocks in the Iskenderun Bay (Northeastern Mediterranean) by genetic and morphological analyzes. Morphometric traits of gilthead sea bream were investigated using a traditional set of measurements and network system. In total, 91 morphometric characters were measured for the Biomorph v3 of measurements and 14 for the truss network system. Results of discriminant function analysis (DF), three functions were produced, and the first two discriminant functions (DFs) explained 90.1% of between-group variability and plotting DF1 (55.2%) and DF2 (34.8%). Mitochondrial DNA sequencing of 16S rRNA region was found to be 830 base pair and 21 bp variable and 3 bp parsimony informative sites between populations. The nucleotide composition was found to be 21.1%, 24.1%, 32.9% and 21.1% for T, C, A and G, respectively. The average value of genetic diversity and genetic distance within populations was found to be 0.00316 and -0.00088, respectively. Haplotype diversity of the cultured population was found to be 0.6710 while the wild population was found to be 0.8333. In the present study, the morphological and molecular analysis identified cultured fish escapees in the wild data set sampled in the aquaculture sites. Despite a mixed gene pool in the wild population, as a result of a long-term culture period of fish originating from Mediterranean broodstocks, the molecular genetics tools identified potential cultured escapees.

Stock structure analysis of the endemic fish, Barbodes carnaticus (Jerdon 1849), for conservation in a biodiversity hotspot.

The population structure of Barbodes carnaticus species was studied using conventional (based on body morphometrics and meristic) and image-based analysis (truss network system) methods. The study was carried out with four stocks, namely Karnataka (KA) and Tamil Nadu (TN) stocks from the River Cauvery, Kerala (KE) stock from the River Chalakudy and farm-reared stock (CI) from Central Institute of Freshwater Aquaculture, Bangalore. A total of 27 morphometric, 9 meristic and 30 truss measurements were used in the study for the stock structure. Fifteen landmarks were used to generate 30 truss distance measurements. The principal component analysis (PCA), factor analysis (FA), discriminant function analysis (DFA) and cluster analysis (CA) were deployed to determine the variation using both the conventional and truss variables. Variations (86.9%) among the morphometric characters were explained by five principal components, while four principal components explain 96.01% of the variation among the truss distances. DFA using conventional method correctly classified 100% of the original grouped classes of the KA, KE and CI and 93.8% of TN stocks. The DFA employed with truss distance was classified into the stocks CI, KA, KE and TN, and the values are 100, 89.1, 8.6 and 6.1%, respectively. Factor analysis based on truss morphometry showed that factor one is related to body shape and factor two is related to head shape. Two clusters were identified in both the conventional and the truss distance analysis. Truss distance-based cluster showed that the KE and CI stocks are similar compared to the TN stock. In contrary, morphometry-based cluster showed the KE and TN stocks are similar compared to CI stock. The multivariate analysis showed that the farm-reared stock (CI) is different from the wild stocks (KA, KE and TN). This study explained that the combination of the conventional and image-based truss network analysis helps to discriminate various stocks of B. carnaticus. Based on the PCA, bilinear data models were generated using R 3.5.3 software for predicting the stock of each individual. Stock discrimination of this species was mainly due to the geographic isolation, river ecology and temperature variations. The stocks of B. carnaticus are highly exploited from the studied rivers, and the species is an important candidate for species diversification to enhance aquaculture production. Within stock variations are found to be minimum in the present morphometric study, hence the gene pool identification and marker study are required for better understanding of the stocks. This stock structure study may help to develop conservation programmes for this endemic species through a more scientific approach.

Truss network based morphometric and meristic variations among south-western populations of Macrognathus aculeatus in Bangladesh

Aim: Landmark-based truss network system was applied to examine the morphometric and meristic differences among four different populaces of Macrognathus aculeatus namely; Dhakuriabeel, Jashore (DBJ); Arial Kha River, Madaripur (AKRM); Nabaganga River, Jhenaidah (NRJ); Kopotakkha River, Jashore (KRJ) in Bangladesh. Methodology: Completely 80 samples of M. aculeatus were collected (20 individuals from each population) for this study. Six meristic characters, 6 morphometric characters and 15 truss-based morphometric characters were measured for one-way analysis of variance (ANOVA) and Tukey-HSD post hoc test. Discriminant function analyses were also performed by using morphometric and truss-based morphometric characters to detect potential characters responsible for population separation. Results: Completely 80 samples of M. aculeatus were collected (20 individuals from each population) for this study. Six meristic characters, 6 morphometric characters and 15 truss-based morphometric characters were measured for one-way analysis of variance (ANOVA) and Tukey-HSD post hoc test. Discriminant function analyses were also performed by using morphometric and truss-based morphometric characters to detect potential characters responsible for population separation. Interpretation: The baseline information derived from the present study would be useful for appropriate conservation of M. aculeatus populations as well as for the researchers for further investigation.

A note on the investigation of morphometric differentiation among mantis shrimp (Stomatopods) in South Madura Waters, Indonesia

Mantis shrimp is one of the fishery resources with a high species variation and wide distribution area that cause differences in morphometric characteristics and population mixing. This study purposed to identify and compare the morphometric characters of mantis shrimp (Stomatopods) as well species kindship in South Madura waters, Indonesia. The study was conducted from October 2019 until January 2020 with specimen collecting from three locations south of Madura. Three types of dominant mantis shrimps caught were chosen for the investigation of morphometric differentiation. Morphometric characters were observed with the standard method and truss network analysis (TNA). Based on certain morphological characters, those mantis shrimp species were Harpiosquilla harpax, Miyakella sp., and Oratosquillina sp. Kruskal Wallis analysis resulted in a difference of 14 from 15 characters with the standard method, and 47 from 56 characters with truss network analysis. Cluster analysis showed that Miyakella sp. and Oratosquillina sp. have a close relationship compared to Harpiosquilla harpax. Moreover, the dominant mantis shrimp populations in the south of Madura formed two species of kindship groups.

A morphometric approach into mackerel (Rastrelliger spp.) diversity in Fisheries Management Area 711 as a management base

Rastrelliger spp. is a small pelagic fish with important ecological and economic value in Indonesia. Genus Rastrelliger has three species with different distribution patterns, so they have diversity in morphology and populations in several waters. The study aimed to analyze the morphometric diversity of mackerel (Rastrelliger spp.) in Bintan, Bangka, and Ketapang waters (Fisheries Management Area 711) using standard morphometric methods and truss network analysis. 310 samples were collected from all locations based on simple random sampling. The growth pattern of Rastrellliger spp. was negative allometric in each location. The cluster analysis showed that the mackerel populations from Ketapang and Bangka formed one group, while Bintan tended to be different. Based on the discriminant analysis, there are overlapping populations between Ketapang and Bangka. The difference of morphometric characters on mackerel (Rastrelliger spp.) can indicate different species, so there is potential for more than one population or stock in FMA 711.

Assessment of morphological variation between stocks of bluefish, Pomatomus saltatrix (Actinopterygii, Perciformes, Pomatomidae), in the Aegean Sea, Black Sea, and Sea of Marmara

The population structure of the bluefish, Pomatomus saltatrix (Linnaeus, 1766), in Turkish waters is scarcely described in the literature. To identify any distinct population units of bluefish, and reaffirm the findings of a previous study, four areas were selected: the Aegean Sea, western Black Sea, eastern Black Sea, and the Sea of Marmara. In this study, truss network morphometrics, meristics, and otolith shape analyses were successfully applied for different population identification of the bluefish. Multivariate analysis of variance (MANOVA) revealed no differences for truss network morphometrics, meristic, and otolith shape characters between males and females. Hence, both sexes were combined for the discriminant function (DFA) and the Principal Component Analysis (PCA). Using univariate ANOVA based on the stepwise method revealed a highly significant difference among different locations for each truss-morphometrics and otolith shape characters. Furthermore, six out of seven meristic characters also showed significant differences between different areas. Based on PCA, 25 out of 27 truss-morphometric characters had a loading value above 0.70, which was considered significant in this study. The results of DFA show clear patterns of truss-morphometric character variations, forming four distinct clusters that were well separated from each other, indicating the existence of four morphologically differentiated populations of the bluefish. The proportion of the correctly classified Aegean Sea, western Black Sea, and eastern Black Sea bluefish samples to their original groups were 100%, demonstrating clear separation of these stocks from each other. Whereas up to 5% of the total samples of the Sea of Marmara were incorrectly classified, assigning to the eastern Black Sea. These findings were supported by meristic and otolith shape characters that also indicated four morphologically differentiated populations of the bluefish. However, their overall proportion of correct classification was relatively lower than the truss-morphometric traits method. The findings suggest the requirement of strategic assessment and management of each bluefish stock separately to use them sustainably in the future.

Stock characterization of Greater Lizardfish, Saurida tumbil (Bloch, 1795) along the west coast of India using morphological and molecular markers

ABSTRACT The Greater Lizardfish, Saurida tumbil, forms a significant fishery in the demersal trawls along the west coast of India. But, the information regarding the stock structure of this species is scarce. In the present study, the morphology of fish, otolith shape, meristic traits and mt-DNA D-loop region were used to delineate the stocks collected from the west coast of India. Samples were collected between October to November 2018 from three major fish landing centres namely Mumbai, Mormugao and Kochi. Truss network analysis and otolith shape indices revealed the morphological divergence for body and otolith shape among the sampling regions. However, meristic traits and D-loop sequences of mitochondrial DNA could not show variation among the stocks, suggesting the existence of a single panmictic population of S. tumbil. The results of the present study are useful to formulate the stock-specific management measures for S. tumbil along the west coast of India.

Morphometric Assessment of Golden Mahseer Populations in the Ganga River Basin, India

River fragmentation has adverse effects on fish populations due to restricted migratory pattern, resulting in risk of population extinction. It has been hypothesized that river fragmentation has led to changes in fish population structure. To test the hypothesis, 149 Golden Mahseer Tor putitora were collected from seven locations, including the Ganga River and upstream and downstream of two impounded headwater tributaries: the Bhagirathi and the Alaknanda rivers. Landmark–based “Truss” and “Geometric” morphometric differentiation was investigated to determine if variations in Golden Mahseer populations exist due to river impoundments. Truss network modeling yielded 90 size–corrected distance variables from 14 landmarks, following which analysis of variance (ANOVA), principal component analysis (PCA) and discriminant function analysis (DFA) were performed. Geometric morphometric modeling included relative warps, PCA, and canonical variate analysis (CVA). Both DFA and CVA plot illustrated overlapping, with DFA suggesting low percentage of classification between populations. Mahalanobis and Procrustes distances extracted from CVA were non‐significant (P &gt; 0.001) among populations. Results indicated the existence of single homogenous population of Golden Mahseer in the Ganga River. This study provides a basis for stock identification useful in developing a suitable plan towards conservation and restoration of Golden Mahseer populations in the fragmented Ganga River.

Intraspecific Phenotypic Variation in Nearly Threatened Mottled Nandus, Nandus nandus (Hamilton, 1822)

Understanding intraspecific phenotypic plasticity is a prerequisite of stock identification, evolutionary studies, sustainable utilization, and fishery conservation. In this study, intraspecific phenotypic plasticity was assessed in terms of the external features (i.e. meristic, morphometric, and truss-based morphometrics) of the wild Nandus populations from four freshwater sources in Southwestern Bangladesh. Fish samples were collected from Arial Kha River, Madaripur (AKRM, n=26); Nabaganga River, Jhenaidah (NRJ, n=22); Bohnni Baor, Gopalganj (BBG, n=26); and Dhakuria Beel, Jashore (DBJ, n=22). Meristic, morphometric, and truss network data were subjected to one-way ANOVA followed by post hoc (Tukey-HSD) test. The meristic counts of all the samples demonstrated significant differences only in one of the six characters. By contrast, significant differences were observed in 8 morphometric characters and 31 truss network data from 16 morphometric characters and 35 truss network data, respectively. Principal component (PCA) and canonical variate analyses (CVA) were also performed on morphometric and truss-based network data. Meristic and morphometric results from PCA and CVA showed that populations were completely intermingled, forming a compact cluster within intrapopulation levels, while truss morphometric characters formed a separate cluster. Three dendrograms independently based on phenotypic relationships among the individuals of the four populations also confirmed the absence of phenotypic differentiation among the population due to clustering of different groups. The baseline information resulting from the current study would be useful for genetic studies and further in situ conservation of Nandus populations in Bangladesh.

Genetic and morphological assessment of a vulnerable large catfish, Silonia silondia (Hamilton, 1822), in natural populations from India.

Silonia silondia is a commercially important fish distributed in Asian countries, which is under threat due to overexploitation. This study focuses on the morphological analysis and genetic variation of S. silondia individuals, through truss network and sequencing of two mitochondrial regions, respectively, from six wild populations of the Ganga and Mahanadi river systems in India. A total of 38 haplotypes was observed by analysing combined mitochondrial genes (cytochrome b + ATPase 6/8) in 247 individuals of S. silondia collected from six populations. Average haplotype and nucleotide diversities were 0.8508 and 0.00231, respectively. Genetic structure analysis showed the predominant cause of genetic variation to be within populations. The two clades were observed among the haplotypes and time of divergence from their most probable ancestor was estimated to be around 0.3949 mya. Analysis of combined mitochondrial genes in six populations of S. silondia resulted into three management units or genetic stocks. The truss network analysis was carried out by interconnecting 12 landmarks from digital images of specimens to identify phenotypic stocks. Sixty-five truss morphometric variables were analysed for geometric shape variation which revealed morphological divergence in River Son specimens. The present study presents molecular markers and genetic diversity data which can be critical input for conservation and management of differentiated populations and future monitoring of the genetic bottleneck. The morphological shape analysis clearly shows that variation in the insertion of adipose fin is an important parameter influencing the morphological discrimination.

Molecular and morphometric divergence of four species of butterflies (Nymphalidae and Pieridae) from the Western Himalaya, India.

Morphometric and molecular divergence among four butterfly species of the families Nymphalidae and Pieridae from the western Himalaya region were investigated using molecular tools, traditional morphometric measures and a truss network system. The considered species were Danaus chrysippus, Vanessa cardui, Pieris brassicae and Pieris canidia. Traditional taxonomy is sometimes unable to discriminate cryptic species or species that have close morphological features. Although taxonomists carefully examine external body features to differentiate the species; however, there is a risk for misidentification during a visual assessment of cryptic species. Therefore, we aimed to use the truss network system of 14 morphological landmarks interconnected to yield 90 variables about molecular taxonomy. Principal component analysis (PCA), discriminant function analysis (DFA) and cluster analysis (CA) were employed to determine morphometric variations. In the traditional analysis, 79, 68, 16 and 5 characters out of 90 were found significant (p < 0.05) for D. chrysippus, V. cardui, P. brassicae and P. canidia, respectively. One to seven principal components were extracted through PCA; they explained 87.5-100% of the total variance in samples. Notably, DFA correctly classified 100% of the original grouped cases and 100% of the cross-validated grouped cases. However, the variations were not the same for the two different methods (truss and traditional) employed for the analysis. We correctly identified all the species; the interspecies sequence divergence was between 0.1034 and 0.1398, and the intra-species sequence divergence range was 0.0001 to 0.0128 using the Cytochrome c oxidase subunit-I (COI) gene. The present studyprovides useful information about the application and complementary role of traditional with truss morphometric analysis for the precise identification and classification of the selected species.

Identifying blue swimming crab (Portunus pelagicus) stocks with truss network analysis approach in Indonesian Fisheries Management Area 712

Exploitation rate of the blue swimming crab (BSC) in Indonesian Fisheries Management Area (FMA) 712 is over-optimum level in 2016. Stocks concern in sustainable management is needed as an effort to maintain its availability. The objective of this study is to identify the stock unit of BSC based on Truss Network Analysis (TNA) of morphometric characters in FMA 712. The BSC was collect in five different locations, i.e. East Lampung, Lancang Island, Cirebon, Rembang, and Southern Madura. Measurements on TNA were carried out at 14 landmark points with 29 characters in carapace to analyze its morphometric characters. The cluster analysis showed that TNA method revealed two stocks units of BSC in FMA 712. The first stock was the BSC population of Southern Madura, and the other stock was the other four population. The longest Euclidean distance was found at Southern Madura indicating similarity level with other populations was low. The discriminant analysis demonstrated the different result. There were three group populations, which every population in one group were able to represent the other population, namely Lancang Island-Cirebon, East Lampung-Rembang, and Southern Madura. Regarding to this study, it is recommended to manage BSC in Southern Madura separately.

Intraspecific phenotype variations in olive barb Systomus sarana (Hamilton, 1822) population from different rivers is possibly linked to locomotive adaptations in caudal fin

Systomus sarana (Hamilton, 1822) [D1] is an economically important food fish species occurring throughout Indian rivers, which also has ornamental value. This study focused on morphological variations in S. sarana from five river basins across India, viz., Godavari, Mahanadi, Krishna, Middle Ganga and Lower Ganga. A truss network was constructed by interconnecting 12 landmarks to generate 65 morphometric variables extracted from digital images of specimens sampled from the study locations. Transformed truss measurements were subjected to Principal component analysis (PCA), Canonical discriminant function analysis (CDFA), Box plot and Thin plate spline (TPS) analyses. PCA identified eight truss variables with significant loadings, while CDFA designated two truss variables with potential for explaining discrimination between populations. Anterior attachment of dorsal membrane from caudal fin was identified to be the most important variable that presented variations across the river basins studied. Discriminant analysis correctly classified 70.5% of the specimens into their original populations. Thin plate spline for morphometric shape variation analysis indicated highest specimen-shape variations (warping) in Mahanadi basin. TPS-principal strain ratio on principal components (PC-1, PC-2) further revealed significant divergence among the populations in five river basins studied. Results of the study revealed variation in stocks of the species, on the basis of shape morphometry. The four significant parameters, differentiating specimens from different basins, were linked to caudal fin origin at dorsal side and the centre and possibly indicate plasticity in response to locomotive adaptations.

Lightweight mechanical metamaterials designed using hierarchical truss elements

Rotating unit systems constitute one of the main classes of auxetic metamaterials. In this work, a new design procedure for lightweight auxetic systems based on this deformation mechanism is proposed through the implementation of a hierarchical triangular truss network in place of a full block of material for the rotating component of the system. Using numerical simulations in conjunction with experimental tests on 3D printed prototypes, the mechanical properties of six types of auxetic structures, which include a range of rotating polygons and chiral honeycombs, were analysed under the application of small tensile loads. The results obtained show that there is almost no difference in the Poisson’s ratios obtained from the regular, full structures and the ones made from triangular truss systems despite the latter, in some cases, being 80% lighter than the former. This indicates that these systems could be ideal candidates for implementation in applications requiring lightweight auxetic metamaterial systems such as in the aerospace industry.

Significance of Traditional and Advanced Morphometry to Fishery Science

Morphometric characters of fishes are measurable or metric characters. Morphometrics is a more or less interwoven set of large statistical procedures to analyze variability in the size and shape of organisms. Morphometrics and phylogenetics of a species are combined to utilize existing phylogeny which addresses hypotheses of shape change through evolutionary time. Morphometric differences among stocks of a species are recognized as important to evaluate population structure and form a basis to identify stocks. Advancements in morphometrics used powerful tools for testing and displaying differences in shape, isolated shape from size variation and identifying stocks of species with unique morphological characteristics enabling better management of the species. Traditional or standard morphometry has been improvised from time to time with advanced methods by technological advancements like geometric morphometrics, image analysis, principal component analysis, truss network analysis and multivariate analysis as well as many more to update knowledge and get more accurate information. These advanced methods have strengthened earlier technologies to improve upgrade fishery research throughout the globe. Doi: 10.28991/HEF-2020-01-03-05 Full Text: PDF

3D face-centered-cubic cement-based hybrid composites reinforced by tension-resistant polymeric truss network

After 30 years of development, 3D printing technology (3DPT) is becoming increasingly popular in civil engineering field. However, weak interlayer performance and lack of reinforcement significantly limit its application. Nowadays, structural-functional integrated energy-efficient building materials, theoretically requiring a balance of mechanical properties and thermal resistance, are an emerging interdisciplinary research area in civil engineering field. This work proposed a novel digital cement-based manufacture technology by integrating 3D printing technology with casting technology. By incorporating tension-resistant polymeric truss network (PTN), a category of 3D face-centered cubic-inspired cement-based hybrid composites (FCHCs) is proposed. Results show the printed PTN can effectively improve the tension-resistant capacity of cement mortar. The FCHCs not only exhibit a multiple cracking damage characteristic but also a low thermal conductivity compared to traditional cement-based materials. A building energy simulation program was performed to evaluate the effectiveness of FCHCs on the energy savings. It is found that 34% heating energy could be saved for FCHCs building.

Morphometric variation of Cork fish (Channa striata Bloch, 1793) from nine populations in Sumatra Island, Indonesia

The geographic isolation and specific character of local habitat could cause variation of morphological characteristics among Cork fish population in different locations. The plasticity of cork fish to adapt to the environment factors possibly has an impact on the different of specific morphological on fish population at different habitat. This study was conducted to investigate the traditional morphometric measurement and truss network differentiation of cork fish populations in different locations at Sumatra Island. Total of 394 cork fish specimens (consisting of 198 males and 196 females) were collected from nine different areas of rivers, swamp and lakes. The samples were analysed based on 14 morphometric (M) and 21 Truss Network Measurement (TNM) characters to find out the significant correlation of the fish on each area sampling. The transformed datasets were proceeded to multivariate testing using Discriminant Function Analysis (DFA) and Cluster Analysis (CA). The results showed that cork fish had 20 distinguishing predominant characters in the head and back of the body. Based on cluster analysis, those fishes were divided into 4 groups depending on geographical isolation except one floodplain population that differs from the other population groups. We revealed that phenotypic dimorphism for female cork fish had a dominant size compared to males in head region and tail fin region. Cork fish in the lake had a dominant body height size compared to rivers and flooded swamps.

MORPHOLOGICAL VARIATION BETWEEN HATCHERY AND WILD ANABAS TESTUDINEUS, BASED ON TRUSS NETWORK ANALYSIS

Several body measurement methods used to identify stock have recently been criticized because of inherent biases and weaknesses. As an alternative, a new system of morphometric measurement called the truss network has been increasingly used for stock identification. Therefore, the morphometric differentiations between wild and captive populations of Anabas testudineus were carried out throughout this study using a truss network. Truss measurements based on anchored at ten homologous landmarks with 19 distances of 120 specimens were measured. Discriminant analysis was performed to investigate distinctions and patterns of morphological variations between wild and captive populations of Anabas testudineus. The findings support the use of the truss network to study the morphological variation among populations as it provides interesting perspectives for the study of biodiversity patterns.

Dataset for the morphological and erythrocytes parameters of Clarias gariepinus, Pangasianodon hypophthalmus, and their reciprocal hybrids.

Discrimination of different fishes can be done through different means which includes morphological appearance. When two fishes are successfully hybridized, they produce progenies that have shared morphology between their pure parent, hence, making morphometric characterization an important aspect of hybrid discrimination. However, erythrocyte characterization is also a simpler method for characterization. The dataset presented in this article represents the traditional morphological data, truss network data and erythrocyte data of pure and novel hybrids from reciprocal crosses of African catfish Clarias gariepinus and Asian catfish Pangasianodon hypophthalmus. Breeding of the broodstocks was done to produce pure and hybrid progenies which were maintained for a period of four to six months. Based on the cross combinations and morphotypes, traditional measurement of twenty-five morphological characters and five meristic counts were recorded. Thereafter pictures of the different fish groups were used to determine values of thirty-six distances between ten landmark points. The morphological abnormality of the hybrids at market size is also presented in this data article for the very first time. Blood was then collected from the caudal peduncle of ten fish per group and smeared on a slide for observation under a compound microscope (at 100 × magnification). Data gotten included erythrocytes parameters such as cell major axis, cell minor axis, nucleus major axis, nucleus minor axis cell area, nucleus area, cell volume, and nucleus volume. Data recording was through the Microsoft excel spreadsheet; which was also used to process the data to get the exclusive ranges of values for paired progenies. The data as presented is associated with the research article “Morphological characterization of the progenies of pure and reciprocal crosses of Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822)” [1]. The dataset presented in this article can be used for easy identification of the novel hybrid progenies of the African Catfish and Asian Catfish.