Tropical Seagrass Research Articles

A preliminary study of carbon dioxide and methane emissions from patchy tropical seagrass meadows in Thailand.

Seagrass meadows are a significant blue carbon sink due to their ability to store large amounts of carbon within sediment. However, the knowledge of global greenhouse gas (GHG) emissions from seagrass meadows is limited, especially from meadows in the tropical region. Therefore, in this study, CO2 and CH4 emissions and carbon metabolism were studied at a tropical seagrass meadow under various conditions. CO2 and CH4 emissions and carbon metabolism were measured using benthic chambers deployed for 18 h at Koh Mook, off the southwest coast of Thailand. The samples were collected from areas of patchy Enhalus acoroides, Thalassia hemprichii, and bare sand three times within 18 h periods of incubation: at low tide at 6 pm (t0), at low tide at 6 am (t1), and at high tide at noon (t2). Seagrass meadows at Koh Mook exhibited varying CO2 and CH4 emissions across different sampling areas. CO2 emissions were higher in patchy E. acoroides compared to patchy T. hemprichii and bare sand areas. CH4 emissions were only detected in vegetated areas (patchy E. acoroides and T. hemprichii) and were absent in bare sand. Furthermore, there were no significant differences in net community production across sampling areas, although seagrass meadows were generally considered autotrophic. Koh Mook seagrass meadows contribute only slightly to GHG emissions. The results suggested that the low GHG emissions from Koh Mook seagrass meadows do not outweigh their role as significant carbon sinks, with a value 320 t CO2 -eq. This study provided baseline information for estimating GHG emissions in seagrass meadows in Thailand.

The contributions of primary sources to fauna and the trophic structure in tropical seagrass areas: A case study from Thai waters

AbstractSeagrass provides habitat and resources for various organisms in coastal areas. However, the productivity of a seagrass meadow might vary by its size, which can influence the contribution of primary sources to fauna and the food web structure. This study uses stable isotopes to investigate the contribution of sources to faunas and the associated food web structure in two tropical seagrass meadows. The carbon (δ13C) and nitrogen (δ15N) stable isotopes of primary sources such as mangrove leaf, coastal and riverine particulate organic matter, seagrass material and associated fauna (fish, bivalves, gastropods, crab, shrimp) were investigated from samples collected seagrass meadows of different sizes, at Libong Island and Tharai Island in Thailand. The contribution of the primary sources to the fauna diet showed that seagrass material was the main food source for fauna in both sites. Moreover, the trophic niche did not overlap among the groups of each fauna guild, reflecting the support seagrass provides to resident fauna. The study emphasizes the important role of seagrass as a habitat and feeding ground in tropical coastal ecosystems.

A Comparative Analysis on Impact of Extraction Methods on Carotenoids Composition, Antioxidants, Antidiabetes, and Antiobesity Properties in Seagrass Enhalus acoroides: In Silico and In Vitro Study.

Enhalus acoroides, a tropical seagrass, is known for its significant contribution to marine ecosystems and its potential health benefits due to bioactive compounds. This study aims to compare the carotenoid levels in E. acoroides using green extraction via ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) and to evaluate the biological properties of these extracts against oxidative stress, diabetes, and obesity through in silico and in vitro analyses. E. acoroides samples were collected from Manado City, Indonesia, and subjected to UAE and MAE. The extracts were analyzed using UHPLC-ESI-MS/MS to identify carotenoids, including β-carotene, lutein, lycopene, β-cryptoxanthin, and zeaxanthin. In silico analysis was conducted to predict the compounds' bioactivity, toxicity, and drug-likeness using WAY2DRUG PASS and molecular docking with CB-Dock2. The compounds C3, C4, and C7 demonstrated notable interactions, with key metabolic proteins and microRNAs, further validating their potential therapeutic benefits. In vitro assays evaluated antioxidant activities using DPPH and FRAP assays, antidiabetic properties through α-glucosidase and α-amylase inhibition, and antiobesity effects via lipase inhibition and MTT assay with 3T3-L1 cells. Results indicated that both UAE and MAE extracts exhibited significant antioxidant, antidiabetic, and antiobesity activities. MAE extracts showed higher carotenoid content and greater biological activity compared to UAE extracts. These findings suggest that E. acoroides, mainly when extracted using MAE, has promising potential as a source of natural bioactive compounds for developing marine-based antioxidant, antidiabetic, and antiobesity agents. This study supplements existing literature by providing insights into the efficient extraction methods and the therapeutic potential of E. acoroides carotenoids.

Trace metal pollution gradients in a tropical seagrass ecosystem

Trace metals are one of the most serious pollutants in tropical seagrass meadows given their persistence and toxicity. Whereas quantity is frequently measured, there is no information on the spatial extent of metal pollution in these systems. Here, we use an island in Indonesia (Barang Lompo) as a model system to study the impact radius of two major and eight trace metals in sediment and seagrass leaves. We provide evidence for exponential decay in both the metal pollution index and concentrations of most metals with increasing distance from the island (k = −0.01 to −0.08 m–1). Consequently, there is an impact radius of approximately 100 m around the island. The comparative analysis of both seagrass species further revealed interspecific differences in metal loads. This study highlights the importance of assessing the spatial extent of metal pollution in addition to its quantity.

Acidification alleviates the inhibition of hyposaline stress on physiological performance of tropical seagrass Thalassia hemprichii

Since the Industrial Revolution, increasing atmospheric CO2 concentrations have had a substantial negative impact influence on coastal ecosystems because of direct effects including ocean acidification and indirect effects such as extreme rainfall events. Using a two-factor crossover indoor simulation experiment, this study examined the combined effects of acidification and hyposaline stress on Thalassia hemprichii. Seawater acidification increased the photosynthetic pigment content of T. hemprichii leaves and promoted seagrass growth rate. Hyposaline stress slowed down seagrass growth and had an impact on the osmotic potential and osmoregulatory substance content of seagrass leaves. Acidification and salinity reduction had significant interaction effects on the photosynthesis rate, photosynthetic pigment content, chlorophyll fluorescence parameters, and osmotic potential of T. hemprichii, but not on the growth rate. Overall, these findings have shown that the hyposaline stress inhibitory effect on the T. hemprichii physiological performance and growth may be reduced by acidification.

Coastal dolphins provide foraging opportunities to benthic-feeding seabirds in a tropical seagrass ecosystem

Abstract In marine ecosystems, predators can affect community and ecosystem dynamics through a variety of processes such as foraging facilitation. Here, we report evidence of foraging facilitation between common bottlenose dolphins (Tursiops truncatus) and double-crested cormorants (Nannopterum auritum) in the Caribbean seagrass-dominated atoll of Turneffe, Belize using aerial drone observations conducted in 2015-2017. While dolphins exhibited occasional aggressive behaviours toward the cormorants, the latter frequently followed dolphin movements, suggesting opportunistic pursuit of dolphins for prey access during dolphin bottom foraging activity. Our observations underscore the intricate ecological relationships among marine predators and highlight the need to quantify the mutual benefits and costs of such interactions as coastal ecosystems are rapidly changing.

High-resolution, precision mapping of seagrass blue carbon habitat using multi-spectral imaging and aerial LiDAR

Blue carbon ecosystems, particularly seagrass meadows, provide a myriad of critical ecosystem services through their role in supporting biodiversity, protecting shorelines, and sequestering and storing carbon. Despite their increased recognition for advancing global marine conservation efforts, our knowledge of the location and spatial extent of seagrass ecosystems globally remains relatively poor, due to the logistical and operational challenges of accurately mapping seafloor habitat using remote sensing techniques. Here we develop and validate an aerial LiDAR approach for mapping tropical seagrass blue carbon habitat in The Bahamas, by integrating full waveform bathymetric LiDAR with multi-spectral imaging. The results demonstrate accuracies between 92 and 98% for predicting seagrass types, over an extensive (e.g., >1000 km2) study area whilst utilizing robust ground-truthing techniques. These findings demonstrate the value of the aerial LiDAR approach used here for ongoing bathymetric surveys of seagrass habitat in The Bahamas and throughout similar ecosystems in the wider Caribbean, which should provide key insights for coastal management and conservation efforts.

Comparative effects of heat stress on photosynthesis and oxidative stress in Halophila ovalis and Thalassia hemprichii under different light conditions

This study investigated the physiological responses of two tropical seagrass species, Halophila ovalis and Thalassia hemprichii, to heat stress under varying light conditions in a controlled 5-day experiment. The experimental design included four treatments: control, saturating light, heat stress under sub-saturating light, and heat stress under saturating light (combined stress). We assessed various parameters, including chlorophyll fluorescence, levels of reactive oxygen species (ROS), antioxidant enzyme activities, and growth rates. In H. ovalis, heat stress resulted in a significant reduction in the maximum quantum yield of photosystem II (Fv/Fm) regardless of the light condition. However, the effects of heat stress on the effective quantum yield of photosystem II (ɸPSII) were more pronounced under saturating light conditions. In T. hemprichii, saturating irradiance exacerbated the heat stress effects on Fv/Fm and ɸPSII, although the overall photoinhibition was less severe than in H. ovalis. Heat stress led to ROS accumulation in H. ovalis and reduced the activity of superoxide dismutase (SOD) and ascorbate peroxidase in the sub-saturating light condition. Conversely, T. hemprichii exhibited elevated SOD activity under saturating light. Heat stress suppressed the growth of both seagrass species, regardless of the light environment. The Biomarker Response Index indicated that H. ovalis displayed severe effects in the heat stress treatment under both light conditions, while T. hemprichii exhibited moderate effects in sub-saturating light and major effects in saturating light conditions. However, the Effect Addition Index revealed an antagonistic interaction between heat stress and high light in both seagrass species. This study underscores the intricate responses of seagrasses, emphasizing the importance of considering both local and global stressors when assessing their vulnerability.

Carbon and nitrogen deposits of macroalgal origin on a tropical seagrass meadow

Carbon and nitrogen deposits of macroalgal origin on a tropical seagrass meadow

Rapid seagrass meadow expansion in an Indian Ocean bright spot

The areal extent of seagrass meadows is in rapid global decline, yet they provide highly valuable societal benefits. However, their conservation is hindered by data gaps on current and historic spatial extents. Here, we outline an approach for national-scale seagrass mapping and monitoring using an open-source platform (Google Earth Engine) and freely available satellite data (Landsat, Sentinel-2) that can be readily applied in other countries globally. Specifically, we map contemporary (2021) and historical (2000–2021; n = 10 maps) shallow water seagrass extent across the Maldives. We found contemporary Maldivian seagrass extent was ~ 105 km2 (overall accuracy = 82.04%) and, notably, that seagrass area increased threefold between 2000 and 2021 (linear model, + 4.6 km2 year−1, r2 = 0.93, p < 0.001). There was a strongly significant association between seagrass and anthropogenic activity (p < 0.001) that we hypothesize to be driven by nutrient loading and/or altered sediment dynamics (from large scale land reclamation), which would represent a beneficial anthropogenic influence on Maldivian seagrass meadows. National-scale tropical seagrass expansion is unique against the backdrop of global seagrass decline and we therefore highlight the Maldives as a rare global seagrass ‘bright spot’ highly worthy of increased attention across scientific, commercial, and conservation policy contexts.

Seasonal effects and trophic pressure shape the responses of species interactions in a tropical seagrass meadow to marine heatwaves

Species interactions are influenced by changes to the environment, such as seasonal variations in temperature, and human‐driven warming including marine heatwaves (MHWs). Alteration of species interactions, particularly those involving foundation species, can shape ecosystem structure, stability and dynamics. Marine habitats, notably seagrass meadows, are threatened by human‐driven environmental changes including MHWs which have the potential to alter trophic interactions through effects on various community members including seagrasses, epiphytic algae, and epiphytic algae grazers. Here we examined the effects of a simulated marine heatwave (control versus + 4°C) in different seasons and grazer occurrence on seagrass traits, epiphytic algae growth, grazer biomass and grazing rate. We found the season in which the MHW occurred affected the seagrass response and grazer influence. In winter, the MHW had positive effects on seagrass growth and nitrogen content and caused significant decreases in epiphytic algae growth. However, in summer, grazer presence increased seagrass growth and biomass, but growth was reduced by the interaction with the MHW. The season in which the MHW occurred affected the magnitude of change in leaf tissue isotopic values and C:N ratio, with greater changes occurring in summer. Epiphytic algal growth was markedly reduced by the interaction between all three factors, leading to the near lack of epiphyte growth in summer with grazers present under the MHW. Summer was also associated with a greater increase in snail biomass (most notably under MHW conditions), and increased snail grazing rate. From these results, we show that winter MHWs can drive increased growth of seagrasses but minimal impacts on grazers, while in summer increased grazer activity can interact with elevated temperatures from a MHW to increase their algal consumption. By examining responses across multiple trophic levels and distinct seasons, we achieve a more representative and realistic depiction of human‐induced environmental impacts on ecosystems.

Monitoring vegetation patterns and their drivers to infer resilience: Automated detection of vegetation and megaherbivores from drone imagery using deep learning

Ecological pattern theory has highlighted spatial vegetation patterns that can be used as indicators of ecosystem resilience. Combining this spatial pattern theory with aerial imagery from drones and automated image processing with deep learning methods, we show how monitoring of natural ecosystems can be enhanced through quantifying vegetation spatial patterns. We demonstrate this approach in a tropical seagrass ecosystem with a high abundance of turtles that generate vegetation patches when grazing. Past field observations suggest that patch size and density reflect the seagrass meadow resilience, but understanding the natural variation in vegetation patchiness is crucial. Employing the deep learning methods of semantic segmentation and object detection, we quantify vegetation patchiness metrics and turtle distribution across 12 ha of seagrass meadow in the years 2012 and 2022. The resulting output facilitates spatial and temporal comparisons, revealing areas of low resilience. In 2012, turtle grazing across the entire site yielded vegetation patch sizes averaging 2 ± 0.2 m2 (95% confidence interval). Reduced patch sizes of 0.24 ± 0.05 m2 and 0.67 ± 0.6 m2 at the reef edge and beach slope respectively, in conjunction with a reduced patch density, indicated lower resilience at the seagrass meadow edges. Analysis of the 2022 dataset indicates a general decrease in patch size over time, suggesting declining resilience. A retraining experiment of the semantic segmentation model was conducted where the initial model was retrained on the 2022 dataset and demonstrated the adaptability of the deep learning methods. Despite using different equipment, the model achieved high accuracy with only 5–10 additional training images. By providing the tools to conduct these analyses, we aim to stimulate the uptake of deep learning for enhancing the data obtained from aerial imagery to improve the monitoring and conservation of natural ecosystems.

A matter of choice: Understanding the interactions between epiphytic foraminifera and their seagrass host Halophila stipulacea

In sub/tropical waters, benthic foraminifera are among the most abundant epiphytic organisms inhabiting seagrass meadows. This study explored the nature of the association between foraminifera and the tropical seagrass species H. stipulacea, aiming to determine whether these interactions are facilitative or random. For this, we performed a "choice" experiment, where foraminifera could colonize H. stipulacea plants or plastic "seagrasses" plants. At the end of the experiment, a microbiome analysis was performed to identify possible variances in the microbial community and diversity of the substrates. Results show that foraminifera prefer to colonize H. stipulacea, which had a higher abundance and diversity of foraminifera than plastic seagrass plants, which increased over time and with shoot age. Moreover, H. stipulacea leaves have higher epiphytic microbial community abundance and diversity. These results demonstrate that seagrass meadows are important hosts of the foraminifera community and suggest the potential facilitative effect of H. stipulacea on epiphytic foraminifera, which might be attributed to a greater diversity of the microbial community inhabiting H. stipulacea.

Paleoecological and paleobiogeographic implications of a seagrass-indicating foralgal skeletal assemblage: Retracing the Burdigalian Quilon Limestone (Kerala Basin, SW India)

Seagrasses are marine angiosperms documented in shallow-marine, soft bottom settings across the Cenozoic. They proliferated globally after their divergence from other alismatid monocots in the late Cretaceous followed by an adaptation to the marine environment. Detailed evaluation of seagrasses in the geological archives is of utmost importance to understand their responses to climatic and environmental alterations in the deep time perspective. Here we examine the lower Miocene Quilon Limestone from the Kerala Basin (southwest India) that encompasses a Pseudotaberina-Halimeda floatstone signalling a robust photozoan foralgal skeletal assemblage. This is characterized by the dominant soritid foraminifer Pseudotaberina and the calcareous green alga Halimeda, in association with other foraminifera and representatives from various biotic groups that indicate a tropical seagrass paleoenvironment. Abundant soritids together with various bryopsidalean Halimeda species indicate light and temperature as the major ecological drivers regulating the Quilon Limestone seagrass paleocommunity during the early Miocene (Burdigalian). The spatio-temporal distribution patterns of Halimeda also indicate temperature as the most prominent ecological constraint determining its dispersal and evolution at multiple latitudes. A well illuminated substrate is envisaged to support the development and calcification of the Halimeda thalli. Abundance of K-strategist foraminifera with minor occurrence of suspension-feeding bryozoans and some gastropods, bivalves indicate a low-nutrient environment.

Identification of anthropogenic source of Pb and Cd within two tropical seagrass species in South China: Insight from Pb and Cd isotopes

Seagrass beds are susceptible to deterioration and heavy metals represent a crucial impact factor. The accumulation of heavy metal in two tropical seagrass species were studied in South China in this study and multiple methods were used to identify the heavy metal sources. E. acoroides (Enhalus acoroides) and T. hemperichii (Thalassia hemperichii) belong to the genus of Enhalus and Thalassia in the Hydrocharitaceae family, respectively. Heavy metal concentrations in the two seagrasses followed the order of Cr > Zn > Cu > Ni > As > Pb > Co > Cd based on the whole plant, and their bioconcentration factors were 31.8 ± 29.3 (Cr), 5.7 ± 1.3 (Zn), 7.0 ± 3.8 (Cu), 3.0 ± 1.9 (Ni), 1.2 ± 0.3 (As), 1.7 ± 0.9 (Pb), 9.1 ± 11.1 (Co) and 2.8 ± 0.6 (Cd), indicating the intense enrichment in Co and Cr within the two seagrasses. The two seagrasses were prone to accumulate all the listed heavy metals (except for As in E. acoroides), especially Co (BCFs of 1124) and Cr (BCFs of 2689) in the aboveground parts, and the belowground parts of both seagrasses also accumulated most metals (BCFs of 27) excluding Co and Pb. The Pb isotopic ratios (mean 208Pb/204Pb, 207Pb/204Pb and 206Pb/204Pb values of 38.2054, 15.5000 and 18.3240, respectively) and Cd isotopic compositions (δ114/110Cd values ranging from –0.09‰ to 0.58‰) within seagrasses indicated the anthropogenic sources of Pb and Cd including coal combustion, traffic emissions and agricultural activities. This study described the absorption characteristics of E. acoroides and T. hemperichii to some heavy metals, and further demonstrated the successful utilization of Pb and Cd isotopes as discerning markers to trace anthropogenic origins of heavy metals (mainly Pb and Cd) in seagrasses. Pb and Cd isotopes can mutually verify and be helpful to understand more information in pollution sources and improve the reliability of conclusion deduced from concentrations or a single isotope.

Population genomics unveils the century-old invasion of the Seagrass Halophila stipulacea in the Mediterranean Sea

The tropical seagrass Halophila stipulacea invaded the Eastern Mediterranean Sea in the late nineteenth century and progressively spread throughout the basin ever since. Its spread is expected to continue north-westward as the Mediterranean Sea becomes warmer, potentially changing the seagrass biogeography of the basin. Given the power of genomics to assess invasion dynamics in non-model species, we report the first ddRAD-seq study of H. stipulacea and small-scale population genomic analysis addressing its century-old Mediterranean invasion. Based on 868 SNPs and 35 genotyped native (Red Sea) and exotic (from Cyprus, Greece, and Italy) samples, results suggest that genetic structure was high, especially between major geographic discontinuities, and that exotic populations maintain comparably lower genetic diversity than native populations, despite 130 years of invasion. The evidence of high heterozygosity excess, coupled with previously reported male-dominated and rare flowering records in the exotic range, suggests that clonal propagation likely played a pivotal role in the successful colonization and spread of H. stipulacea in the Mediterranean. This shift in reproductive strategy, particularly evident in the Italian populations located closest to the western boundary and representing more recent establishments, underscores the importance of this cost-effective mode of reproduction, especially during the initial stages of invasion, raising questions about the species future expansion trajectory. Our findings serve as a catalyst for future research into the species’ invasion dynamics, including deciphering the intricate roles of acclimatization and rapid adaptation, important for a comprehensive assessment of invasion risks and improving management strategies aimed at conserving seagrass ecosystems.

Diversity and density of marine intertidal gastropods in tropical seagrass beds at Oransbari Bay, South Manokwari - West Papua

Gastropod communities are one of the important components of the food chain in seagrass meadows. This study aims to investigate the diversity of gastropods in the distribution of the seagrass beds at Oransbari Bay. This study was carried out in November 2022, using the line transect method. Data collection is carried out at three stations, where each station is divided into three transects. A total of 36 species consisting of 16 families and 5 orders, were recorded in this study. The total density of gastropods found in the waters of Oransbari Bay ranged from 5.77-9.43 ind/m2, with an average of 6.99 ± 2.10 ind/m2. Conus sp. has the highest density value of any gastropod. Meanwhile, for seagrasses, five species from four clans and two families were obtained. The percent cover of seagrass ranges from 17.05 ± 3.30% – 58.90 ± 22.90% with an average of 40.18 ± 21.3%. Halophila minor and Thallasia hempricii showed a positive correlation with gastropod density. The value of the gastropod diversity index is in the medium category, the gastropod evenness index is in the stable category, and the gastropod dominance index is in the low category. The results of this study provide a base for a proper gastropod checklist at Oransbari Bay. Future research is recommended that involves a more detailed studies on the feeding ecology and habitat preference of the gastropods.Keywords:DiversityDistributionGastropodsSeagrass bedsOransbari bay

Plant growth and development of tropical seagrass determined rhizodeposition and its related microbial community

In the recent study, we investigated the seasonal variations in root exudation and microbial community structure in the rhizosphere of seagrass Enhalus acoroides in the South China Sea. We found that the quantity and quality of root exudates varied seasonally, with higher exudation rates and more bioavailable dissolved organic matter (DOM) during the seedling and vegetative stages in spring and summer. Using Illumina NovaSeq sequencing, we analyzed bacterial and fungal communities and discovered that microbial diversity and composition were influenced by root exudate characteristics s and seagrass biomass, which were strongly dependent on seagrass growth stages. Certain bacterial groups, such as Ruegeria, Sulfurovum, Photobacterium, and Ralstonia were closely associated with root exudation and may contribute to sulfur cycling, nitrogen fixation, and carbon remineralization, which were important for plant early development. Similarly, specific fungal taxa, including Astraeus, Alternaria, Rocella, and Tomentella, were enriched in spring and summer and showed growth-promoting abilities. Overall, our study suggests that seagrass secretes different compounds in its exudates at various developmental stages, shaping the rhizosphere microbial assemblages.

BIOACTIVE COMPOUNDS, PHENOL CONTENT AND ANTIOXIDANT ACTIVITY OF TROPICAL SEAGRASS Halodule pinifolia

Intertidal seagrasses are exposed to a high level of sunlight, temperature, and desiccation daily, which can lead to increase in reactive radical species producing. In order to survive, they may produce some bioactive compounds and may change the content of nutrient and non-nutrient. In this experiment, tropical seagrass Halodule pinifolia collected from the coastal area of Pangumbahan Sukabumi Indonesa was evaluated their potential nutritional value and natural antioxidant compound. Fresh seagrass H. pinifolia contained ash, protein and fat of 14.89, 9.74 and 2.13 g/100 g dry matter; whereas the amounts of soluble dietary fiber, insoluble dietary fiber were 12.84 and 27.23 g/100 g dry matter, respectively. The highest content of total phenol was found in ethyl acetate extract (0.31 mg GAE/g dry matter), followed by methanol and hexane extracts of 0.18 and 0.12 mg GAE/g dry matter, respectively. The extract of ethyl acetate also had the highest activity on DPPH-scavenging measured by IC50 value of 214.38 ppm in compared to methanol and hexane extracts. All of extracts contained bioactive compounds of steroid, flavonoid and phenol hydroquinone; whereas triterpenoid was only found in the extract of hexane.

Temporal and spatial variations of air-sea CO2 fluxes and their key influence factors in seagrass meadows of Hainan Island, South China Sea

Seagrass ecosystems have received a great deal of attention for contributing to uptake of atmospheric CO2, and thereby helping to mitigate global climate change (‘blue carbon’). Carbon budgets for seagrass ecosystems are developed by estimating air-sea CO2 fluxes. Data for air-sea CO2 flux for tropical seagrass ecosystems are lacking, which is problematic for constraining global seagrass carbon budgets. Here, we sought to address this important data gap for tropical seagrass ecosystems (dominated by Thalassia hemprichii and Enhalus acoroides) from the Hainan Island of South China Sea, while also testing what the main factors driving the variations of air-sea CO2 fluxes are. We found that air-sea CO2 fluxes exhibited a U-shape diurnal variability from 6 a.m. to 6 a.m. of the next day, with the highest and lowest air-sea CO2 fluxes values at early morning and afternoon, respectively. Biological processes were the driving force for mediating diurnal variations of seawater pCO2. The pCO2, sea in different seasons displayed a trend of increasing from spring, reaching maximum in summer and then a decreasing trend after summer, where water temperature, wind speed and seagrass growth mainly drove the variations. This resulted in net uptake of CO2 in all seasons except during summer in our study seagrass ecosystems, with greater negative values found in autumn (−3.63 ± 0.76 mmol m−2 d−1) than those in winter (−2.84 ± 0.60 mmol m−2 d−1). While the nutrient loading induced seagrass biomass changes (especially the seagrass T. hemprichii), which mediated the air-sea CO2 fluxes changes among different seagrass meadows. Net annual CO2 uptake potential under low nutrient loading (−0.77 ± 0.16 mol m−2 yr−1) was 23–54 % greater than high nutrient loading seagrass meadows, with the average annual air-sea CO2 flux of the three seagrass meadows as −0.64 ± 0.13 mol m−2 yr−1. These results suggest that tropical seagrass meadows of Hainan Island are a significant CO2 sink of atmospheric CO2, but this capacity can be diminished by nutrient loading. Scaling up, we estimate the annual atmospheric CO2 uptake by seagrass meadows of Hainan Island (total area 55.28 km2) was 1544 t of CO2 yr−1, equivalent to the annual emissions from the wholesale, retail, accommodation and catering industries of 164,000 tourists in Hainan Island. With carbon neutrality becoming an important part of global climate governance, this study provides timely information for capitalising on the ability of seagrasses to contribute to natural climate solutions.