Trivedi Effect Research Articles

Elasticity Profile of Skin, Neuronal, Cardiac, and Skeletal Muscle Cells after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation

The present study aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on elasticity of skin, heart, muscle, and neuronal cells in the H9C2 (rat cardiomyocytes), C2C12 (mouse myoblast cells), HaCaT (human keratinocytes), and SH-SY5Y (human neuroblastoma cells) cell line in DMEM medium. The test formulation constituents were divided into two parts; one section was defined as untreated test formulation (UT), while the other portion of test formulation received Biofield Energy Healing/Blessing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing/Blessing Treatment and divided as Biofield Energy Treated/Blessed (BT) and untreated (UT) test items. MTT data showed that the test formulation in various concentrations was found as safe and nontoxic in the tested concentrations with viability range from 73% to 307%. Young’s modulus (YM) is a measure of cell stiffness, a decrease in YM value indicates increase elasticity of the cells and vice-versa. YM in H9C2 cells were decreased by 9.6% and 66.1% in the BT-DMEM + UT-TI group at 0.1 and 1 µg/mL respectively, as compared with untreated test group. However, C2C21 cells showed increased YM by 443.9% at 1 µg/mL in the UT-DMEM + BT-TI group, while 869.6% increased YM in the BT-DMEM + UT-TI group at 1 µg/mL as compared with untreated test group. However, 314% increased YM was reported in the BT-DMEM + BT-TI group at 1 µg/mL as compared with the untreated test group. However, the value of YM was significantly decreased in the HaCaT cell line by 247.7% (at 1 µg/mL), 225.8% (at 0.1 µg/mL), and 97.9% (at 1 µg/mL) in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. In addition, YM was significantly decreased in the SH-SY5Y cell line by 92.6%, 18.1%, and 26.6% at 1 µg/mL in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. Overall, the results showed the significant decreased YM among the SH-SY5Y, HaCaT, and H9C2 cells, while it was increased in the C2C21 cell line. Thus, the mechanical properties of cells such as cellular function, including shape, motility, differentiation, division, and adhesion to its surrounding extracellular matrix were improved. Overall, it can be useful in many disease progressions with improved cellular elasticity and its associated complications/symptoms.

Evaluation of Immunomodulatory Effect of a Novel Test Formulation in D-Galactose-Induced Aging Dysfunction in Sprague Dawley Rats

The aim of the study was to evaluate the immunomodulatory activity of the Biofield Treated/Blessed proprietary test formulation consisting of essential ingredients viz. minerals (zinc, magnesium, iron, and copper) and vitamins (B6, B12, and D3) in male Sprague Dawley rats. Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Healing Treatment/Blessing, while the other part was defined as the Biofield Energy Treated/Blessed sample, which received the Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi remotely. Additionally, three group of animals were also received Biofield Energy Treatment per se (at day -15) under similar conditions. The parameters were assessed such as immune biomarkers (IgM, IgG, IgA, IgE, CD4+, CD8+, and CD28+), biochemistry and hematology and histopathology. The experimental results showed IgG level was significantly increased by 10.70% and 8.03% in the G6 (Biofield Energy Treatment per se at day -15) and G8 (Biofield Treatment per se to animals plus Biofield Treated test formulation from day -15) groups, respectively as compared with untreated test formulation (G4). Additionally, CD8+ count was significantly increased by 20.67% in the G8 group, while CD28+ count was significantly increased by 11.70%, 8.32%, and 9.82% in the G7 (Biofield Energy Treated test formulation at day -15), G8, and G9 (Biofield Treatment per se (day -15) to animals plus untreated test formulation) groups, respectively after Biofield Energy Treatment to the animals as compared with the untreated test formulation. In hematological analysis, platelet count was increased in the G5, G6, G7, G8, and G9 groups by 40.69%, 27.95%, 26.67%, 38.58%, and 28.28%, respectively compared with the disease control (G2) group. Biochemical parameters showed significant decrease in the level of creatinine by 32.14% in the G9 group as compared with the G2 group. Further, animal body weight, feed intake, relative organ weight, and histopathological findings of all the tested groups did not show any abnormal findings with respect to the safe and non-toxic treatment strategies. Overall, the experimental data concluded that the Biofield Energy Treated/Blessed test formulation showed considerable improved cellular and humoral immune response as compared with the untreated test formulation. Thus, the Trivedi Effect®-Biofield Energy Healing Treatment per se and the test formulation has the significant capacity for immunomodulatory effect, stress management and anti-aging by improving overall health.

Effect of Biofield Treatment on Blood, Hepatic, and Cardiac Biomarkers in Vitamin D3 Deficiency Diet (VDD) Induced Sprague Dawley Rats

A novel proprietary formulation was designed that consist of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The study objective was to evaluate the impact of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for immunomodulatory activity. The test formulation was divided into two parts. One part was denoted as the untreated test formulation without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of total leukocytes count (TLC) were significantly increased by 23.1% and 17.44% in the Biofield Energy Treated test formulation (G5) and Biofield Energy Treated test formulation from day -15 (G7) groups, respectively compared with the disease control group (G2) induced by VDD. Moreover, the level of neutrophils was significantly increased by 71.68%, 92.92% (p≤0.01), 42.48%, and 49.56% in the G5, G7, Biofield Energy Treatment per se plus Biofield Energy Treated test formulation from day -15 (G8), and Biofield Energy Treatment per se animals plus untreated test formulation (G9) groups, respectively compared with the G2 group. Monocyte level was also increased by 23.53%, 17.65%, and 29.41% in the G5, Biofield Energy Treatment per se to animals from day -15 (G6), and G9 groups, respectively compared to the G2 group. Hepatic biomarker like alkaline phosphatase (ALP) was significantly reduced by 14.98% and 23.07% (p≤0.05) in the G7 and G9 groups, respectively as compared to the G2 group. Besides, cardiac biomarker like creatine kinase myocardium band (CK-MB) was also significantly (p≤0.05) reduced by 20.99% in the G6 group compared with the G2. Altogether, results suggested that the Biofield Treated test formulation and Biofield Energy Treatment per se significantly increased immune-related parameters, which might be beneficial for the management of immune-compromised patients as well as to boost-up the immunity in healthy peoples. The results showed a significant slowdown of disease progression and all other disease-related complications/symptoms in the preventive Biofield Energy Treatment group per se and the Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control group.

Structural Characterization and Isotopic Abundance Ratio Analysis of the Consciousness Energy Healing Treated Ofloxacin

Ofloxacin is an antibiotic, useful against the number of bacterial infections. This scientific investigation was performed to identify the impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of ofloxacin using sophisticated analytical techniques. Ofloxacin sample was divided into control and treated parts. Only the treated ofloxacin received the Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The LC-MS spectra of both the samples of ofloxacin at retention time 3 minutes exhibited the mass of the protonated molecular ion peak at m/z 362.17 (M+H)+. The chromatographic peak area% of the treated ofloxacin (52.4%) was increased by 2.03% compared to the control sample (51.36%). The LC-MS based isotopic abundance ratio of PM+1/PM in the Biofield Treated ofloxacin was significantly increased by 22.43% compared with the control sample. Similarly, the GC-MS based isotopic abundance ratio of PM+1/PM in the Biofield Treated ofloxacin was significantly increased by 19.24% compared with the control sample. The LC-MS and GC-MS based isotopic abundance ratio of PM+1/PM (2H/1H or 15N/14N or 13C/12C or 17O/16O) was significantly increased in the Biofield Treated ofloxacin as compared to the control sample. Thus,2H, 15N, 13C, and17O contributions from (C18H21FN3O4)+ to m/z 363.17 in the treated ofloxacin were significantly increased compared with the control sample. The increased isotopic abundance ratio of the Trivedi Effect®-Consciousness Energy Healing Treated ofloxacin may increase the intra-atomic bond strength and increase its physical stability. The new form of treated ofloxacin would be more stable, better soluble, and bioavailable compared to the control sample. It would be more useful to design efficacious pharmaceutical formulations that might offer better therapeutic response against infections in the urethra, urinary tract, gonorrhoea, pneumonia, infectious diarrhoea, bronchitis, cellulitis, bacterial infection of the eye and ear, multidrug-resistant tuberculosis, prostatitis, otitis media, plague, etc.

Study of the Consciousness Energy Healing Treatment and It’s Effect on the Isotopic Abundance Ratio of Ascorbic Acid (Vitamin C)

Ascorbic acid is a water-soluble vitamin (Vitamin C) essential for both the plants and animals for the metabolic process. In this study, the liquid chromatography-mass spectrometry (LC-MS) analytical technique was used to characterize the structural properties and isotopic abundance ratio to evaluate the effect of the Trivedi Effect®-Consciousness Energy Healing Treatment on L-ascorbic acid compared to the control sample. The ascorbic acid sample was divided into control and treated parts. Only the treated part received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Mahendra Kumar Trivedi. The control and treated samples showed a chromatographic peak at retention time (Rt) 1.8 minutes exhibited the deprotonated molecular ion peak at m/z 175 (M-H)- (calculated for C6H7O6-, 175.02) in the mass spectra. The peak area of the treated sample (12817614.01) was significantly increased by 8.81% compared to the control sample (11779918.9). The LC-MS based isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in the treated ascorbic acid was significantly increased by 23.22% compared with the control sample. Thus,13C, 2H, and17O contributions from (C6H7O6)- to m/z 176 in the treated ascorbic acid were significantly increased compared with the control sample. The increased isotopic composition of the treated ascorbic acid might have altered the neutron to proton ratio in the nucleus. The changes in isotopic abundance could be due to changes in nuclei possibly through the interference of neutrino particles via the Trivedi Effect®-Consciousness Energy Healing Treatment. The increased isotopic abundance ratio and peak area of the treated ascorbic acid may increase the intra-atomic bond strength and its stability. This novel ascorbic acid after the Trivedi Effect®-Consciousness Energy Healing Treatment would be very useful to design more efficacious pharmaceutical formulations against scurvy, obesity, cardiovascular diseases, hypertension, rheumatoid arthritis, Alzheimer's disease, cancer, etc.

Evaluation of the Isotopic Abundance Ratio of Consciousness Energy Healing Treated L-Tryptophan Using LC-MS Spectrometry

L-tryptophan is an essential α-amino acid, necessary for the normal growth in newborns, nitrogen balance in adults, protein synthesis, precursor of serotonin, melatonin, niacin, and albeit inefficiently in human, also the precursor of indole alkaloids and auxins in plants. This current study was designed to investigate the impact of the Trivedi Effect®-Biofield Energy Healing Treatment (Blessing) on the structural properties and the isotopic abundance ratio of L-tryptophan using LC-MS analytical technique. L-tryptophan sample was divided into two parts, one part of L-tryptophan was considered as the control sample (no Biofield Energy Treatment was provided), while the second part was treated with the Trivedi Effect®-Consciousness Energy Healing Treatment/Blessing remotely by a renowned Biofield Energy Healer, Dahryn Trivedi and termed as the treated sample. The mass spectra of both the control and treated samples with respect to the chromatographic peak at retention time (Rt) 2.1 minutes exhibited the mass of the molecular ion peak adduct with hydrogen ion at m/z 205.08 (calcd for C11H13N2O2+, 205.1), along with low molecular fragmented mass peaks at m/z 188, 159, and 102 for C11H12N2O2+, C10H11N2+, and C8H6+, respectively were also observed. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14Nor17O/16O) in the treated L-tryptophan was significantly increased by 35.93% compared with the control sample. Hence,the 13C, 2H, 15N, and 17O contributions from C11H13N2O2+ to m/z 206.08 in the treated L-tryptophan was significantly increased compared to the control sample. It could be hypothesized that the changes in the isotopic abundance and mass peak intensities due to the modification in nuclei possibly through the interference of neutrino particles using the Trivedi Effect®-Consciousness Energy Healing Treatment. The Biofield Energy Treated/Blessed L-tryptophan with increased stable isotopic abundance ratio might have changed the physicochemical properties with higher force constant in the molecule. The new form of treated L-tryptophan would be a better and more stable in the supplements, nutraceutical, and pharmaceutical formulations, which would be advantageous for the prevention and treatment of pellagra, depression, kynurenine. It could also maintain the normal label of tryptophan and avoid increase of its metabolite, lower the neurotoxin and a metabotoxin behavior, glutaric aciduria type I (glutaric acidemia type I) disorder, eosinophilia-myalgia syndrome (EMS), incurable and sometimes fatal flu-like neurological condition, etc. As tryptophan is the precursor for the plant hormones like indole alkaloids and auxins, hence, this treated L-tryptophan would be advantageous for the improvement of yield, productivity, and quality of crops and other plants.

Characterization of the Consciousness Energy Healing Treated Cholecalciferol Using LC-MS and GC-MS Spectrometry

Vitamin D3 (cholecalciferol) is a fat-soluble vitamin, which widely used for the prevention and treatment rickets, osteoporosis, arthritis, Parkinson’s and Alzheimer’s diseases, autoimmune disease, dementia, glucose intolerance, etc. The impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of cholecalciferol were evaluated using LC-MS and GC-MS spectroscopy. The test sample cholecalciferol was divided into control and treated parts. Only, the treated cholecalciferol was received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the samples at retention time (Rt) ~22 minutes exhibited the mass of the molecular ion peak at m/z 385.25 (calcd for C27H45O+, 385.35). The LC-MS based isotopic abundance ratio of PM+1/PM in the treated cholecalciferol was increased by 0.74% compared with the control sample. But, the GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated cholecalciferol was significantly increased by 66.39% and 62.69%, respectively compared with the control sample. Hence,13C, 2H, 17O, and 18O contributions from C27H44O+ to m/z 386 and 387 in the treated cholecalciferol were significantly increased compared with the control sample. The isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O) and PM+2/PM (18O/16O) in the treated cholecalciferol were significantly increased as compared to the control sample. The increased isotopic composition of the Trivedi Effect®-Consciousness Energy Healing Treated cholecalciferol might have altered the neutron to proton ratio in the nucleus via the possible mediation of neutrino. The increased isotopic abundance ratio of the treated cholecalciferol may increase the intra-atomic bond strength, increase its stability. The new form of cholecalciferol would be better designing novel pharmaceutical formulations that might be more stable and more efficacious for the prevention and treatment of various diseases such as vitamin D deficiency, rickets, osteoporosis, arthritis, multiple sclerosis, cancer, diabetes mellitus, mental disorders, cardiovascular diseases, hypertension, infections, influenza, cognitive impairment in older adults, Parkinson’s and Alzheimer’s diseases, autoimmune disease, dementia, glucose intolerance, multiple sclerosis, etc.

Evaluation of Biofield Energy Healing Based Test Formulation on Thyroid Biomarkers in Vitamin D3 Deficiency Diet (VDD) Induced Animal Model

This present study evaluated the impact of Consciousness Energy Healing Treatment (the Trivedi Effect) on a novel test formulation in maleSprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for the estimation of thyroid biomarkers such as calcitonin, parathyroid (PTH),thyroid stimulating hormone (TSH), and thyroxine (T4). A novel proprietary test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The novel test formulation was divided into two parts; one part was defined as untreated test formulation, while the other of test formulation and the three group of animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of calcitonin (pg/mL) was significantly increased by 22.7%, 4.2%, 44.9%, 72%, and 67.7% (p≤0.01) in the Biofield Energy Treated test formulation group (G5), Biofield Energy Treatment per se (G6), 15 days pre-treatment of Biofield Energy Treated test formulation (G7), 15 days pre-treatment of Biofield Energy Treated test formulation to the Biofield Energy Treated animals (G8), and Untreated test formulation to the Biofield Energy Treated animals (G9) groups, respectively as compared with the untreated group (G4). However, the level of TSH was reported to be decreased by 23.3%, 20.4%, and 37.3% in the G7, G8, and G9 respectively as compared with the G4. The level of PTH was decreased by 30.6% and 10.9% in the G6 and G9, respectively, while it was increased by 9.7% in the G5 as compared with the G4. Similarly, the level of T4 was increased by 40.5%, 20.7%, and 20.8% in the G5, G7, and G8 groups respectively as compared with the G4. In conclusion, the Biofield Treated test formulation and Biofield Energy Treatment per se significantly improved the thyroid biomarkers in serum that can be used against many thyroid disorders such hyperthyroidism, goitre, thyroid nodules, thyroid cancer, thyroid hormone resistance, Hashimoto’s thyroiditis, and many more. Overall, the results showed the significant slowdown the disease progression and disease-related all other complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) compared with the disease control group. Keywords: Biofield Treatment; Thyroid Biomarkers; The Trivedi Effect; Thyroxine; Vitamin D3 deficiency diet; PTH; Thyroid Disorders

Antioxidant Activity of Biofield Treated Proprietary Test Formulation Supplemented with Vitamins and Minerals in Vitamin D3 Deficiency Diet (VDD) Induced Sprague Dawley Rats

A proprietary formulation was designed that consisted minerals (zinc, magnesium, iron, calcium, selenium, and copper), vitamins (pyridoxine HCl, cyanocobalamin, ascorbic acid, and cholecalciferol), Panax ginseng extract, and cannabidiol isolate. The study was aimed to assess the potential of the novel test formulation (blessed) and per se to the animals with the Trivedi Effect® in male Sprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD). The test formulation consisted above mentioned ingredients was divided into two parts. One part was left aside as the untreated test formulation without any Biofield Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Treatment by renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of lipid peroxidation end product malondialdehyde (MDA) in liver tissues was significantly reduced by 34.59%, 34.91%, and 65.81% (p≤0.001) in test formulation treated with Biofield Energy (G5), Biofield Treated test formulation from day -15 (G7), Biofield Treatment per se with Biofield Treated test formulation from day -15 (G8) groups, respectively as compared to the disease control group (G2). Moreover, level of catalase enzyme in liver tissues was also increased by 8.64% in the G7 group as compared to the G2 group. Besides, in brain homogenate the level of glutathione peroxidase (GPx) was significantly increased by 433.94%, 266.97%, 133.94%, 467.89%, and 489.86% in the G5, Biofield Energy Treatment per se to animals from day -15 (G6), G7, G8, and Biofield Treatment per se animals plus untreated test formulation (G9) groups, respectively than G2. Antioxidant enzyme-like superoxide dismutase (SOD) was significantly (p≤0.001) increased by 14.16% in the G9 group as compared to the G2 group. Allover, results signified that the Biofield Treated test formulation significantly increased antioxidative parameters, could be able to give support against oxidative stress induced by free radical and to maintain a good human health.

Evaluation of Vitamin D3 metabolite (25-OH Vit D3), Neurotransmitter (ACh), and the Expression of Proinflammatory Cytokines (IL-6 and TNF-α) in Tissue Homogenate after Administration of Biofield Energy Healing-based Novel Proprietary Test Formulation and Biofield Treatment per se to the Animals in Vitamin D3 Deficiency Diet (VDD)-induced Sprague Dawley Rats

A novel proprietary test formulation was designed which included minerals, vitamins, β-carotene, cannabidiol isolate,and Panax ginseng extract. This present study was evaluated the impact of the Trivedi Effect® on novel proprietary test formulation in male Sprague Dawley rats, fed with vitamin D3 deficiency diet (VDD). The novel test formulation was divided into two parts; one part was defined as untreated test formulation, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of 25-OH Vit. D3 was measured in brain homogenate, which was found to be increased by 20.13%, 24.12%, 45.86%, 14.79%, and 29.96% in the G5 group treated with Biofield Treated test formulation, Biofield Energy Treatment per se to the animals (G6), 15 days pre-treatment of Biofield Energy Treated test formulation (G7), Biofield Energy Treatment per se plus Biofield Energy Treated test formulation from day -15 (G8), and untreated test formulation to the Biofield Energy Treated animals (G9) groups respectively, as compared with the disease control (G2) group. Brain acetylcholine (ACh) level was increased by 61.33% in the G7 group as compared with the untreated test formulation (G4) group. The expression of interleukin-6 (IL-6) was significantly reduced by 43.44% (p≤0.01), 30.93%, 21.42%, 45.99% (p≤0.01), and 60.85% (p≤0.01), respectively as compared with the G4. Lung pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α) level was significantly reduced in the G5, G6, G7, and G8 by 24.86%, 32.55% (p≤0.01), 30.12% (p≤0.01), and 42.69% (p≤0.01), respectively, as compared with the G4 group. Altogether, the Biofield Treated test formulation and/or per se treatment to the animals significantly improved the levels of active form of vitamin D3 metabolite (25-OH Vit D3) and neurotransmitter (ACh); consequently significantly lowered the expression of proinflammatory cytokines (IL-6 and TNF-α). Therefore, the energized test formulation or per se treatment could be effectively useful against neuronal damage and inflammation for the management of brain disorders such as Alzheimer’s disease, dementias, brain cancer, epilepsy and other seizure disorders, mental disorders, and Parkinson’s. Thus, the results showed a significant slowdown of disease progression and all other disease-related complications/symptoms in the preventive Biofield Energy Treatment group per se and the Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control group.

Evaluation of Anti-oxidation and Therapeutic Effect of Biofield Energy Healing Based Novel Test Formulation Using TNBS (Tri Nitro Benzene Sulfonic Acid) - Induced Ulcerative Colitis in Sprague Dawley Rats

The aim of the study was to evaluate the antioxidant potential of Biofield Energy Healing (the Trivedi Effect®) based test formulation using TNBS-induced colitis animal model. Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was treated with Biofield Energy Treatment by Mr. Mahendra Kumar Trivedi and defined as the Biofield Energy Treated test formulation. The colon tissue was used for the estimation of anti-oxidation activity for catalase (CAT), glutathione (GSH), lipid peroxidation (LPO) product, myeloperoxidase (MPO), superoxide dismutase (SOD), and glutathione peroxidase (GPx) using standard procedure. The antioxidant results showed that the CAT level was significantly increased by 95.4% (p≤0.001), 72.3%, 47.6%, and 13.9% in the Biofield Energy Treated test formulation (G5), Biofield treatment per se to animals (-15 days)(G6), Biofield Energy Treatment per se to animals plus Biofield Energy Treated test formulation (-15 day) (G8), and Biofield Energy Treatment per se to animals plus untreated test formulation (G9) groups, respectively as compared to the untreated test formulation group (G4). Further, colon GSH activity was found to be significantly increased by 23.2% (p≤0.05) 15.4%, and 15.5%, in G5, G6, and G9 groups, respectively with respect to G2 group. In addition, colon LPO activity data suggested that it was decreased by 12%, 17%, 18%, and 19.1% in G5, G6, Biofield Energy Treated test formulation (-15 day) (G7), and G8 groups, respectively, as compared with the G2 group. The level of MPO showed a significant (p≤0.001) reduced level by 27.9%, 22%, 14.5%, 16.6%, and 25.3% in G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. The level of colon SOD was increased by 16.7% and 14.2% in the G5 and G9 groups, respectively as compared with the untreated test formulation, G4 group. Colon GPx level was increased by 177.6%, 71.4%, 71.4%, 161.2%, and 114.3% in G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. Thus, it can be concluded that the Trivedi Effect®-Consciousness Energy Healing based test formulation and Biofield Energy per se has significant colon anti-oxidation profile, which can be used to improve many autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Assessment of Physical, Thermal and Spectral Properties of Consciousness Energy Treated Cholecalciferol

Cholecalciferol (vitamin D3) is used nowadays in nutraceuticals regarding the prevention and treatment of vitamin D deficiency and associated diseases. This study was done to analyse the effect of the Trivedi Effect® - Energy of Consciousness Treatment on the physicochemical, thermal, and spectral properties of cholecalciferol using PSA, PXRD, DSC, TGA/DTG, FT-IR, and UV-Vis analysis. For this study, the cholecalciferol sample was divided into control/ untreated and Biofield Energy Treated vitamin D3. The treated vitamin D3 sample received Biofield Energy Treatment (the Trivedi Effect®) remotely for ~3 minutes by Mr. Mahendra Kumar Trivedi, who was located in the USA, while the test samples were located in the research laboratory in India. The treated sample was designated as the Biofield Energy Treated sample. The PSA analysis showed that the particle size values at d10, d50, d90, and D(4, 3) of the treated sample were significantly decreased by 5.80%, 16.49%, 17.52%, and 16.23%, respectively compared to the control sample. However, the specific surface area of the treated cholecalciferol was significantly increased by 7.26% compared to the control sample. Besides, the PXRD analysis revealed that the relative intensities regarding the characteristic diffraction peaks in the treated sample were significantly altered from -42.56% to 22.42%, along with -41.69% to 72.71% alterations in the crystallite sizes, compared with the control sample. Also, the treated sample showed 2.80% decrease in the average crystallite size. The DSC analysis showed a slight increase (0.24%) in the melting point of the treated sample along with 3.68% increase in the latent heat of fusion (ΔH) compared to the control sample. Also, the decomposition temperature of the treated sample was decreased by 0.29%, whereas the ΔH was increased by 5.79%, compared to the control sample. Moreover, the TGA/DTG analysis revealed the significant decrease in weight loss in the 1st and 3rd step of degradation of the treated sample by 18.58% and 89.81%, respectively, along with 1.83% increase in the maximum thermal degradation temperature compared with the control sample. Overall, the thermal stability of the treated cholecalciferol sample was observed to be increased in comparison to the control sample. Thus, the Trivedi Effect® might be used to produce a different polymorph of cholecalciferol, which possesses the improved qualities in terms of appearance, dissolution, absorption, bioavailability, and thermal stability as compared with the untreated sample. Thus, the Biofield Energy Treated cholecalciferol might be used in designing of better nutraceutical and pharmaceutical formulations possessing improved therapeutic response regarding the treatment of vitamin D deficiency associated diseases.

Investigation on the Impact of Biofield Energy Treatment on the Physical, Thermal and Spectroscopic Characteristics of Zinc Chloride

Zinc is an essential trace mineral that plays an important role in the human body as a regulator for different enzymatic systems. It is used in various supplements in the form of zinc chloride to meet the daily requirements. This study was designed with the aim to investigate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment on the various physical, thermal, and spectral properties of zinc chloride. The study involves dividing the zinc chloride sample in two parts, followed by keeping the first part untreated and named it the control sample. Besides, The Trivedi Effect®- Biofield Energy Healing Treatment was provided to the other part remotely by the renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and the sample was termed as Biofield Energy Treated sample. Later on, both the samples were analyzed using PXRD, PSA, DSC, UV-Vis, and FT-IR analytical techniques. The PXRD analysis revealed the significant changes in the crystallite sizes (from -66.68% to 25.02%) and the relative peak intensities (from -81.35% to 141.22%) along with 26.46% decrease in the average crystallite size of the Biofield Energy Treated sample, compared with the control sample. Additionally, the treated sample showed reduction in the particle sizes d10, d50, d90, and D(4,3) values by 92.18%, 23.24%, 14.48%, and 29.31%, respectively as compared to the control sample. However, the surface area of the treated sample was observed to be significantly increased by 224.57%, compared to the control sample. The DSC analysis revealed slight increase in the melting and decomposition temperature of the Biofield Energy Treated sample by 0.84% and 3.62%, respectively; along with the significant increase in the latent heat of melting and decomposition by 24.85% and 123.23%, respectively, compared to the control sample. It showed that the thermal stability of the treated sample was significantly increased. However, the UV-Vis and FT-IR studies revealed that the structural properties of the control and treated samples were remained same. The overall study represents that The Trivedi Effect®- Energy of Consciousness Healing Treatment may be used to generate a polymorphic form of zinc chloride, which might possess enhanced solubility, dissolution and bioavailability profile along with improved thermal stability, compared to the control sample. Such properties of the treated zinc chloride may ensure its efficacy, stability and safety during the process of shipment, handling, and storage; thus, The Trivedi Effect®-Energy of Consciousness Healing Treated zinc chloride would be useful for designing the better formulation with enhanced bioavailability, stability and efficacy profile.

Evaluation of Physicochemical, Spectral and Thermal Properties of Energy of Consciousness Healing Treated Iron Sulphate

Iron sulphate is used in the treatment of iron deficiency anaemia and other chronic disorders such as heart and kidney diseases. This study has the objective to analyze the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment on the physicochemical, spectroscopic and thermal properties of iron sulphate using various analytical techniques. In this, the test compound, i.e., iron sulphate was divided into two parts; one as control (without Biofield Energy Treatment), and the other as Biofield Energy Treated, which received the Biofield Energy Treatment remotely by the renowned Healer, Mr. Mahendra Kumar Trivedi. The PXRD analysis of the Biofield Energy Treated sample showed significant alterations in the range of -39.49 to 301.40% in the relative intensities, and from -15.40 to 33.36% in the crystallite sizes, compared with the control sample. The average crystallite size of the treated sample was also increased by 4.98% as compared to the control sample. The particle sizes in the treated sample at d10, d50, d90 and D(4,3) values were significantly increased by 67.12%, 47.72%, 33.18% and 42.01%, respectively; whereas, the specific surface area was significantly reduced by 38.39%, compared with the control sample. The TGA thermograms showed three steps of thermal degradation in which, the weight loss of Biofield Energy Treated sample in the first and second step was reduced by 5.82% and 16.09%, respectively, while, it was increased by 6.78% in the third step, compared to the control sample. The total weight loss in the treated sample was also reduced by 2.76%, along with slight alteration in the maximum thermal decomposition temperature, compared with the control sample. The DSC analysis showed the decrease in the melting temperatures of the 1st, 2nd and 4th peaks by 8.24%, 19.29%, and 0.61%, respectively, while 4.57% increase in the 3rd peak of the treated sample, compared with the control sample. The latent heat of fusion (ΔH) corresponding to the 1st, 2nd, 3rd and 4th peaks of the treated sample also showed alterations by -92.29, -86.29, 60.92, and 6.37%, respectively, compared with the control sample. The Trivedi Effect®-Consciousness Energy Healing Treatment might produce a novel polymorphic form of iron sulphate having increased crystallite and particle size along with enhanced thermal stability. It may help in improving the quality, safety and stability during the process of handling, storage, and shipment of the iron sulphate with better therapeutic response against iron deficiency anaemia.

Physicochemical, Thermal and Spectroscopic Characterization of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract

Ashwagandha root extract is widely used in nutraceutical preparations due to its broad range of pharmacological activities. The objective of this study was to analyse the impact of Biofield Energy Treatment (The Trivedi Effect®) on the physicochemical, thermal and spectral properties of ashwagandha root extract by using various analytical techniques. The sample of ashwagandha root extract was divided into two parts in which one part was kept untreated and called as control sample; while the other part was provided with the Consciousness Energy Healing Treatment remotely by the Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and termed as Biofield Energy Treated sample. The study reported that the particle size values at d10, d50, d90, and D (4,3) in the Biofield Energy Treated sample was significantly decreased by 9.23%, 9.04%, 11.86%, and 10.59%, respectively compared with the control sample. Thus, the surface area of the treated sample was significantly increased by 10.75% compared to the control sample. The PXRD analysis showed that the control as well as the Biofield Energy Treated samples was amorphous in nature. The DSC analysis showed that the onset evaporation temperature and latent heat of vaporization were significantly altered by 2.18% and-10.29%, respectively in the Biofield Energy Treated sample compared with the control sample. The TGA analysis indicated the four step thermal degradation of both the samples. The 1st, 2nd, 3rd, and 4th steps of thermal degradation of the treated sample showed an alteration in the weight loss by -6.59%, 1.97%, -1.80%, and -8.65%, respectively along with 0.81% reduction in the total weight loss, compared with the control sample. Besides, the maximum thermal degradation temperature (Tmax) in the control sample was observed at 234.17°C and 365.12°C for the two broad peaks, which was decreased by 1.73% and 0.82%, respectively compared to the control sample. The overall analysis suggests that the Energy of Consciousness Healing Treatment might enhance the solubility, absorption, and bioavailability profile of ashwagandha root extract along with altered thermal stability. Such altered properties might help in designing better pharmaceutical and nutraceutical preparations; thereby provide better therapeutic response against various diseases such as amnesia, arthritis, anxiety, cancer, impotence, neurodegenerative, and cardiovascular diseases.

Investigation of Impact of Biofield Energy Healing Treatment on Physicochemical, Thermal and Spectral Properties of Magnesium Gluconate

Magnesium plays important role in the functioning of cells, nerves, muscles, and bones of human body. It is incorporated in the form of magnesium gluconate in various pharmaceutical and nutraceutical supplements due to its wide applications. This study was done with the objective to analyse the effect of The Trivedi Effect®- Energy of Consciousness Healing Treatment on the physical, thermal, and spectral properties of magnesium gluconate. In this regard, the sample of magnesium gluconate was divided in two parts, in which first part was kept untreated and termed as control sample. Besides, the second part was remotely treated by the renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi with The Trivedi Effect®- Biofield Energy Healing Treatment and termed as Biofield Energy Treated sample. Consequently, both the samples were analyzed using PXRD, PSA, DSC, TGA/DTG, UV-Vis, and FT-IR analytical techniques. The PXRD analysis revealed that the crystallite sizes and relative peak intensities of the treated sample showed variations in the range of-25.01% to 185.79% and 6.99% to 32.99%, respectively along with 13.03% increase in average crystallite size as compared to the control sample. The particle sizes d10, d50, d90, and D(4,3) values of the treated sample were significantly decreased by 3.70%, 21.88%, 22.03%, and 21.16%, respectively, along with 10.75% increase in the surface area as compared to the control sample. The DSC studies showed minor decrease in melting point (0.10%) with 3.74% increase in the latent heat of fusion of the treated sample, compared to the control sample. Besides, The TGA/DTG analysis represented alterations in the weight loss pattern and maximum thermal degradation temperature (Tmax) of the treated sample. The analysis revealed three steps thermal degradation, in which the variation was observed from -6.79% to 10.14% in terms of weight loss along with 0.24% increase in the total weight loss of the treated sample, compared to the control sample. The Tmax of the first two peaks were found to be increased by 6.90% and 1.28%, while 3rd and 4th peaks were slightly decreased by 0.57% and 0.24% in the treated sample, compared to the control sample. The Energy of Consciousness Healing Treatment might produce a new polymorphic form of magnesium gluconate, which may have a better solubility, absorption and bioavailability profile along with altered thermal stability compared to the untreated sample. Thus, the Biofield Energy Treated magnesium gluconate would be helpful in designing the better nutraceutical/pharmaceutical formulations with better therapeutic responses against the magnesium deficiency.

Chromatographic and Spectroscopic Evaluation of the Consciousness Energy Healing Treated Ashwagandha Root Extract

Ashwagandha (Withania somnifera) extract is a popular health supplement, used in many nutraceutical and pharmaceutical formulations for good health benefits along with the prevention and treatment of various human diseases. The purpose of this study was to evaluate the impact of The Trivedi Effect®-Consciousness Energy Healing Treatment on the characteristic properties of the phyto-compounds present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts, one part of the sample was termed as untreated/control sample, while the other part of the sample received The Trivedi Effect®-Biofield Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and termed as Biofield Energy Treated sample. The LC-ESI-MS data revealed that the chromatographic peak area% of the phytoconstituents present in the treated sample was significantly altered in the range of 31.13% to 20.61% compared to the control sample. Similarly, the average peak area of the treated sample was significantly improved by 20.61% compared to the control sample. A total of 14 withanolides such as viscosa lactone B, dihydrowithanolide D, withanolide A, withanolide B, etc. were proposed with m/z 489, 473, 471, and 455 at the retention times (Rt ) of 13.2, 14.5, 14.8, and 18.2 minutes, respectively. At Rt 13.2 minutes (m/z: 489), the peak area% and mass peak intensity of the phytocompound in the treated ashwagandha were significantly increased by 2.57% and 14.83%, respectively compared with the control sample. Similarly, at Rt 14.5 minutes (m/z: 473), peak area% was increased by 3.76%, but the mass peak intensity was significantly decreased by 26.48% in the treated sample compared with the control sample. At Rt 14.8 minutes (m/z: 471), peak area % was same, but the mass peak intensity was increased by 2.74% in the treated ashwagandha compared with the control sample. Subsequently, at Rt 18.2 minutes (m/z: 455), peak area% was increased 1.01%, whereas the peak intensity was significantly decreased by 10.90% in case of the treated sample compared to the control sample. These findings intensify to assume that The Trivedi Effect®-Consciousness Energy Healing Treatment might be responsible for the overall improvement of relative concentrations and altered relative abundance of the phytoconstituents in the ashwagandha root extract compared to the control sample. This Biofield Energy Treated ashwagandha root extract would be helpful to design better nutraceutical/pharmaceutical formulations against inflammatory diseases, immunological disorders, sexual disorders, arthritis, stress, cancer, diabetes, aging, and other chronic infections.

The LC-MS and GC-MS Based Isotopic Abundance Ratio Analysis of the Consciousness Energy Healing Treated Silver Sulfadiazine

Silver sulfadiazine is a topical antibiotic helps to prevent and treat wound infections in patients with severe burns. This research was performed to evaluate the impact of the Trivedi Effect®- Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of silver sulfadiazine using spectroscopic and chromatographic techniques. The test sample silver sulfadiazine sample was divided into control and treated parts. Only the treated part of silver sulfadiazine was received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the samples at retention time (Rt ) 4.1 minutes exhibited the mass of the protonated molecular ion peak at m/z 251.08 (calculated for C10H11N4 O2 S+ , 251.06) was found to be sulfadiazine. The chromatographic peak area of the treated silver sulfadiazine was significantly increased by 20.35% compared to the control sample. The result indicated that the solubility profile of the treated silver sulfadiazine must have increased significantly after the Biofield Energy Treatment compared with the control sample. The LC-MS based isotopic abundance ratio of PM+1/PM in the treated sulfadiazine was significantly increased by 31.88% compared with the control sample. Similarly, the GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated sulfadiazine was significantly increased by 9.74% and 15.13%, respectively compared with the control sample. Hence, 13C, 2 H, 15N, 17O, 33S, and 34S contributions from (C7 H9 N2 O2 S)+ to m/z 186 and 187 in the treated sulfadiazine were significantly increased compared to the control sample. Thus, the isotopic abundance ratios of PM+1/PM (2 H/1 H or 13C/12C or 15N/14N or 17O/16O or 33S/32S) and PM+2/PM (34S/32S) in the treated sulfadiazine were significantly increased compared to the control sample. The changes in isotopic abundance and peak area might be due to changes in nuclei possibly via the interference of neutrino particles controlled by the Trivedi Effect®-Consciousness Energy Healing Treatment. The increased isotopic abundance ratio of the treated silver sulfadiazine might improve the chemical bond strength, led to increase the stability in the body. This novel silver sulfadiazine would be more efficacious in pharmaceutical formulations which might offer better solubility, bioavailability, and therapeutic response against bacterial infections, superficial and partial thickness burn injuries, etc.

Isotopic Abundance Ratio Analysis of the Consciousness Energy Healing Treated Flutamide using LC-MS and GC-MS Spectrometry

Flutamide is used to treat prostate cancer, which acts by blocking the action of exogenous testosterone binding through its androgen receptor. This study was designed and performed to investigate the impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of flutamide using LC-MS and GC-MS spectroscopy. Flutamide sample was divided into control and treated parts. Only the treated flutamide received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a famous Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the samples at retention time (Rt ) 3.4 minutes exhibited the mass of the deprotonated molecular ion peak at m/z 275.08 [M-H]-. The peak area of the treated sample was significantly increased by 92.42% compared to the control sample, which indicated that the solubility profile of the treated sample was significantly increased compared to the control sample. The LC-MS based isotopic abundance ratio of PM+1/PM (2 H/1 H or 13C/12C or 15N/14N or 17O/16O) in the treated flutamide was significantly increased by 59.48% compared with the control sample. The GC-MS based isotopic abundance ratio of PM+1/ PM in the treated flutamide sample was increased by 10.39% compared with the control sample. The results indicated that the 13C, 2 H, 15N, and 17O contributions from (C11H11F3 N2 O3 )+ to m/z 277 in the treated sample were significantly increased compared with the control sample. The isotopic abundance ratio of PM+1/PM (2 H/1 H or 13C/12C or 15N/14N or 17O/16O) in the treated flutamide was significantly increased compared to the control sample. The changes in the peak area and isotopic abundance could be due to changes in nuclei possibly through the interference of neutrino via the Trivedi Effect®-Consciousness Energy Healing Treatment. The new form of treated flutamide was formed that might have increase the chemical bond strength, stability, solubility, bioavailability, and efficacy, which could be very much useful to design more efficacious pharmaceutical formulations against prostate cancer, androgen-dependent skin and hair conditions including acne, seborrhea, hirsutism, and scalp hair loss, hyperandrogenism, as well useful for feminizing hormone therapy aimed at transgender women.

The Effect of Novel Proprietary Test Formulation for Immunomodulatory activity by Assessing the CD Biomarkers and Colon Microscopy in TNBS-induced Ulcerative Colitis (UC) Rats Model

The aim of the present study was to evaluate the immunomodulatory action of Biofield Energy Healing (the Trivedi Effect®) Treatment to the animals and to the novel proprietary test formulation in tri-nitro benzene sulphonic acid (TNBS)-induced male Sprague Dawley rats. The test formulation consists of minerals (zinc, magnesium, iron, and copper) and vitamins (B6 , B12, and D3 ). Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Healing Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Additionally, three group of animals were also received Biofield Energy Treatment per se under similar conditions. The cellular biomarkers like cluster of differentiation-4 (CD4+ ), CD8+ , and CD28+ , biochemistry and hematology along with histopathologic profile were evaluated. The percentage of CD4+ count was increased by 8.87% in the G6 (Biofield Energy Treatment per se at day -15) group as compared to the untreated test formulation (G4) group. While the expression of CD8+ count was increased by 10.12% and 13.01% in the G6 and G7 (Biofield Energy Treated test formulation at day -15) groups, respectively as compared with the disease control (G2) group. Moreover, the level of CD28+ was significantly (p≤0.001) increased by 15.50% in the G5 (Biofield Energy Treated test formulation) group compared to the G2 group. In hematological analysis, platelet count was increased in the G7 by 8% compared with the G4. The level of uric acid was decreased by 40.47%, 13.8%, and 14.28% in the G6, G8 (Biofield Energy Treatment per se to animals plus Biofield Energy Treated test formulation at -15 day), and G9 (Biofield Energy Treatment per se to animals plus untreated test formulation) groups, respectively as compared with the G4 group. Histopathologic findings of all the tested groups did not show any abnormal findings with respect to the safe and nontoxic treatment strategies, which indicated that, the test formulation and Biofield Energy Treatment per se ameliorates colon inflammation in the TNBS-induced colitis rats by decreasing histopathologic lesions. Overall, the experimental data concluded that the Biofield Energy Treated test formulation showed considerable improved cellular and humoral immune response as compared with the untreated test formulation. Thus, the Trivedi Effect®-Biofield Energy Healing Treatment per se and the test formulation considerable improved cellular immune response, immunomodulatory effect, and gut health function.