Abstract

A proprietary formulation was designed that consisted minerals (zinc, magnesium, iron, calcium, selenium, and copper), vitamins (pyridoxine HCl, cyanocobalamin, ascorbic acid, and cholecalciferol), Panax ginseng extract, and cannabidiol isolate. The study was aimed to assess the potential of the novel test formulation (blessed) and per se to the animals with the Trivedi Effect® in male Sprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD). The test formulation consisted above mentioned ingredients was divided into two parts. One part was left aside as the untreated test formulation without any Biofield Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Treatment by renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of lipid peroxidation end product malondialdehyde (MDA) in liver tissues was significantly reduced by 34.59%, 34.91%, and 65.81% (p≤0.001) in test formulation treated with Biofield Energy (G5), Biofield Treated test formulation from day -15 (G7), Biofield Treatment per se with Biofield Treated test formulation from day -15 (G8) groups, respectively as compared to the disease control group (G2). Moreover, level of catalase enzyme in liver tissues was also increased by 8.64% in the G7 group as compared to the G2 group. Besides, in brain homogenate the level of glutathione peroxidase (GPx) was significantly increased by 433.94%, 266.97%, 133.94%, 467.89%, and 489.86% in the G5, Biofield Energy Treatment per se to animals from day -15 (G6), G7, G8, and Biofield Treatment per se animals plus untreated test formulation (G9) groups, respectively than G2. Antioxidant enzyme-like superoxide dismutase (SOD) was significantly (p≤0.001) increased by 14.16% in the G9 group as compared to the G2 group. Allover, results signified that the Biofield Treated test formulation significantly increased antioxidative parameters, could be able to give support against oxidative stress induced by free radical and to maintain a good human health.

Highlights

  • The human has a good antioxidant defense system to protect the cells from oxidative injury by the free radical to the cell membranes, lipids, proteins, and nucleic acids [1]

  • The current research work was designed with the aim of investigating the antioxidant potential of Biofield Energy Healing Treated test formulation supplemented with minerals and vitamins in Sprague Dawley rats

  • Lipid peroxidation is the process in which the membrane bound enzymes, proteins, and receptors are inactivated through loss of cell membrane integrity [37]

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Summary

Introduction

The human has a good antioxidant defense system to protect the cells from oxidative injury by the free radical to the cell membranes, lipids, proteins, and nucleic acids [1]. The current research work was designed with the aim of investigating the antioxidant potential of Biofield Energy Healing (the Trivedi Effect®) Treated test formulation supplemented with minerals and vitamins in Sprague Dawley rats. The National Center of Complementary and Integrative Health (NCCIH) has recommended CAM health care approach including Biofield Therapy in addition to other practices such as Qi Gong, rolfing structural integration, Tai Chi, Ayurvedic medicine, deep breathing, yoga, aromatherapy, natural products, chiropractic/osteopathic manipulation, massage, meditation, hypnotherapy, special diets, naturopathy, homeopathy, guided imagery, progressive relaxation, acupuncture, acupressure, healing touch, relaxation techniques, pilates, movement therapy, mindfulness, traditional Chinese herbs and medicines, essential oils, Reiki, cranial sacral therapy and applied prayer (as is common in all religions, like Buddhism, Judaism, Hinduism, and Christianity)

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