The present study was carried out to investigate the effectiveness of pyrolytic oil obtained from Triplochion scleroxylon sawdust which used as a preservative against fungi attack on Alstonia bonnei, Ceiba pentandra, Pterocarpus osun, Terminalia randii, Triplochiton scleroxylon wood. The wood samples used for the experiment were dimensioned into 20 x 20 x 60 mm and soaked for 72 hours to obtain appreciable absorption. The percentage absorption after soaking and weight loss after exposure to termite were determined. Data were analyzed using simple statistics and analysis of variance at α0.05. The highest and lowest percentage absorption was recorded for Terminalia randii and Alstonia bonnie with 23.20 % and 13.10 % respectively. Analysis of variance result indicates the significant difference in wood species used. The result obtained for weight loss revealed significant differences in wood species as well as in fungi also. The result further showed that those are taken as control recorded the highest weight loss to white and brown rot fungi. Pyrolytic oil possesses a great potential in the prevention of fungi attack as it contained phenolic compound.