This paper presents a mathematical-statistical method to help evaluate the temperature effect on BOD and SS residual ratio during and after a trickling filter plant treatment. Mathematical relationships are developed and brought to workable linear forms which are then used for statistical examination by the F test method. The data for this purpose is provided by regular measurements of wastewater quality in a full-scale treatment plant. While settling performance did not respond consistently to temperature changes, the secondary treatment and the overall plant performance provided good correlations with the correct representative temperatures.