This study was aimed to investigate the green biosynthesis of silver nanoparticles (AgNPs) by Escherichia coli (E. coli) and Aspergillus fumigatus (A. fumigatus). The effects of AgNPs on selected microbial species including their own pathogens in vitro were examined. In addition, experimental infection in a healthy she camel was done by culture of Trichophyton verrucosum isolate from the natural infection. The animal was kept in a separate pen until the development of skin lesions. Afterwards, prepared fungal AgNPs was applied topically to cover the whole infected area. The treatment was done twice daily for six days. The current findings revealed that, AgNPs biosynthesis was fast and formed within 7 and 10 minutes for A. fumigatus and E. coli, respectively. Photometric analysis of the liquid media obtained from both microorganisms containing silver ion showed a peak around 430-450nm and 420-450nm, respectively. TEM micrographs indicated formation of well-dispersed AgNPs ranged from 3.4-26.4nm and 64.9nm for A. fumigatus and E. coli, respectively. Zetasizer analysis indicated that AgNPs produced by A. fumigatus and E. coli average was 125.3 nm and 182.9 nm, respectively. Inhibition zone diameter and minimum inhibitory concentration (MIC) of AgNPs produced by A. fumigatus was larger and lower, respectively than that of AgNPs produced by E. coli and relevant antibiotics and antifungal for all studied microorganisms. Experimental infection with Trichophyton verrucosum induced alopecia, erythema, numerous small subcutaneous nodules and brownish blackish crusts with hyperkeratosis of infected she camel. The treatment with current prepared fungal AgNPs induced a pronounced relieve and disappearance of lesions. The current study concluded that fungal and bacterial synthesised AgNPs have antibacterial and antifungal effect even against their own pathogens. However, A. fumigatus AgNPs was more efficient than that of E. coli due to smaller particle size and subsequent higher penetrating ability. The fungal AgNPs led to disappearance of skin lesions that resulted from experimental infection with Trichophyton verrucosum. Long term studies are recommended to investigate the most effective dose of fungal AgNPs against different fungal infection.