Biomedical or surgical devices undergo numerous bone structure modification processes, which require enhanced tribo-mechanical properties in biological fluid environments to avoid corrosive processes. Therefore, the following research shows a detailed study of the tribological response of [β-TCP/HA]n multilayer coatings deposited on AISI 302 steels, in order to simulate biomedical devices subjected to friction processes in biological fluid. Mechanical study determined that the coating with 50 bilayers showed an increase 26.3% in elastic modulus of and 107.7% in hardness compared to the coating with 1 bilayer. Subsequently, the tribological evaluation in a dry and biological fluid environment (Hartmann's solution) showed a reduction in the friction coefficient of 36.7% and 43.5%, respectively, when comparing the n = 50 bilayers with the n = 1 bilayer. This study concludes that a coating with n = 50 bilayers is superior to n = 1 bilayer when used as a protective coating on biomedical devices.