Triad Approach Research Articles

Groundwater quality assessment of one former industrial site in Belgium using a TRIAD-like approach

Contaminated industrial sites are important sources of pollution and may result in ecotoxicological effects on terrestrial, aquatic and groundwater ecosystems. An effect-based approach to evaluate and assess pollution-induced degradation due to contaminated groundwater was carried out in this study. The new concept, referred to as “Groundwater Quality TRIAD-like” (GwQT) approach, is adapted from classical TRIAD approaches. GwQT is based on measurements of chemical concentrations, laboratory toxicity tests and physico-chemical analyses. These components are combined in the GwQT using qualitative and quantitative (using zero to one subindices) integration approaches. The TRIAD approach is applied for the first time on groundwater from one former industrial site located in Belgium. This approach will allow the classification of sites into categories according to the degree of contaminant-induced degradation. This new concept is a starting point for groundwater characterization and is open for improvement and adjustment.

Integrated assessment of trace pollutants associated with the Korean coastal environment: Exampled from the sediment TIE and triad approaches

Here we present our triad approach followed by major findings based on sediment pollution studies that determined the chemical concentration, biological effect, and community structure associated with trace pollutants in the Korean coastal environment. Since the late-1990s, over several hundred sites along the Korean coast have been surveyed including sediment (including pore-water), water, and biological samples and analyzed for instrumental quantification, in vitro and in vivo bioassays, and benthic community indices. The instrumental data of persistent heavy metals and organic pollutants in Korean sediments generally suggest that concentrations of target pollutants are relatively low to moderate but their distribution is widespread across the coasts. The compositional pattern and spatial distribution suggests that their sources are independent of each other. However, there are local zones with extremely high concentrations of certain pollutants indicating point sources. In order to search for the relationship between chemical concentrations and biological responses, some selected data (n=123) were further analyzed in terms of mass balance analysis (sediment-TIE towards TEQ approach). Most of the dioxin-like activities were observed in the fractions containing dioxins, furans, and certain polycyclic aromatic hydrocarbons (PAHs) and about half of the samples showed significant estrogenic activities in the fractions of PAHs and alkylphenols. Direct relationship(s) between chemical and ecotoxicological data could not be fully addressed between component(s). However, a positive relationship between them became apparent in some cases, indicating a general good agreement between chemical stresses and biological responses. Overall, we found that a sediment triad approach for an integrated assessment of trace pollutants in the coastal environment was extremely useful. It would be much more powerful when all the components are fully and simultaneously analyzed and interpreted.

Balancing Multiple Objectives Using a Classification-Based Forest Management System in Changbai Mountains, China

Contemporary forest management often consists of multiple objectives, including restoration of human-impacted forested landscapes toward their range of natural variability (RNV) and sustainable levels of timber production. Balancing multiple management objectives is often challenging due to intrinsic conflicts between these objectives and a lack of reference conditions for evaluating the effectiveness of forest restoration efforts. We used a spatially explicit forest landscape model to assess how well a classification-based forest management (CFM) system could achieve multiple objectives in a Korean pine broadleaf mixed forest ecosystem at Changbai Mountain in Northeast China. The CFM system divided the forest landscape into three management areas (Commercial Forest, Special Ecological Welfare Forest, and General Ecological Welfare Forest), each with its own management objectives and prescriptions, but with an overall goal of increasing the ecological and economic sustainability of the entire landscape. The zoning approach adopted in the Chinese CFM system is very similar to the TRIAD approach that is being advocated for managing public forests in Canada. In this study, a natural disturbance scenario and seven harvest scenarios (one identical to the current harvest regime and six alternative scenarios) were simulated to examine how tree species composition, age structure, and timber production at the landscape level can be affected by different strategies under the CFM system. The results indicated that the current forest management regime would not only fail to reach the designated timber production level but also move the forest landscape far away from its RNV. In order to return the currently altered forest landscape to approach its RNV while providing a stable level of timber production over time, harvest intensities should be reduced to a level that is equivalent to the amount of timber removals that would occur under the natural disturbances; and the establishment of forest plantations is also required.

Wastewater canal Vojlovica, industrial complex Pancevo, Serbia: Preliminary ecotoxicological assessment of contaminated sediment

Effluents collected from the industrial complex of Pancevo, Serbia (oil refinery, petrochemical plant, and fertilizer factory) are discharged into a wastewater canal entering the Danube River. In this study, which was focused on sediment assessment, a complex triad approach consisting of chemical analysis, sediment toxicity tests and macrozoobenthos community analysis was applied. In toxicity tests on sediment elutriates, the following responses were registered - stimulatory effect in algal bioassay, no effect in acute test with Daphnia magna, and low to moderate toxicity in the conventional Vibrio fischeri test. Moderate to high toxicities were recorded in solid phase tests on Myriophyllum aquaticum and V. fischeri. High content of Hg, certain PAHs and noncharacterised sediment contaminants accumulated over years contribute not only to the registered toxicity, but also to the complete absence of macrozoobenthos. The obtained results proved that regularly measured conventional and priority pollutants are hardly ever the only toxic contaminants present in sediments. Toxicity tests, in particular the contact test, might guide towards a better selection of parameters to be regularly or occasionally monitored. In addition, complete sediment toxicity tests proved to be an appropriate method for assessing the bioavailability of the chemically detected contaminants. The analysis of the macrozoobenthos composition and structure as inevitable part of sediment risk assessment procedures integrates the effects of multiple stressors and gives a realistic insight into not only sediment contamination by toxic pollutants, but also the sediment status in general.

A Comparison of Pairs, Triads and Quads in Multi-Attribute Decision Making

The pair-wise comparison technique is a common approach for completing multi-attribute evaluations. However, this approach has limitations, especially for larger attribute sets, where the use of the technique is time-consuming because it requires a relatively large number of comparisons. The authors conducted an experiment to test the efficacy of three alternative approaches for eliciting preferences, specifically pairs, triads and quads. Ninety-three subjects used one of the three approaches to rank the importance of fifteen items. The results indicate that those employing the pair-wise approach took significantly longer than those using the triad or quad approach. In addition, the triad technique yielded more accurate results (compared to the pair and quad methods). Finally, the quad approach generated fewer intransitivities than the pair-wise or triad approaches. No differences were observed across the three techniques with respect to reliability or perceived ease of use. Implications are provided for both practitioners and researchers.

Expediting sustainable brownfields redevelopment by applying Triad using the membrane interface probe

AbstractRedevelopment and reuse plans are often based upon an expedited delineation and remediation life cycle, since delayed reuse usually has economic consequences. It has also become increasingly important to utilize sustainable practices to achieve investigation and remediation goals. In this article, the Triad approach is used to expedite the delineation of a source area within a municipal landfill to complete the remedial effort prior to construction of an urban civic center. The Triad approach uses the three elements of systematic project planning, dynamic work strategy, and real‐time measurement to expedite site characterization (Interstate Technology and Regulatory Council, 2003). In this article, the Triad sampling strategy consisted of two phases. The first phase included in situ screening of soil and groundwater using the membrane interface probe (MIP), and the second phase included confirmatory sampling via vertical profiles in the soil and groundwater. This study found that, using the MIP in a dynamic sampling strategy, a critical element of the Triad approach, combined with the proper placement of confirmatory samples, significantly reduced overall project cost and will expedite the site redevelopment. The use of the Triad approach also contributed to the integration of green and sustainable practices into the project. © 2010 Wiley Periodicals, Inc.

Effects of herbicides and freshwater discharge on water chemistry, toxicity and benthos in a Uruguayan sandy beach

Environmental water chemistry analysis and microcosm toxicity bioassays (MTB) were performed to assess lethality of herbicides on the mole crab Emerita brasiliensis in a sandy beach affected by a freshwater discharge (Andreoni canal) from rice crops. A 5-yr macrocosm field sampling (MFS) was conducted to evaluate freshwater effects on population abundance. Propanil was only detected at the inner portion of the Andreoni canal (IAC), whereas quinclorac and clomazone were found at the IAC and at the canal mouth (CM). A major propanil metabolite was detected at the CM. Herbicides were undetectable at 13 km from the CM. MTB showed an increased susceptibility to propanil with decreasing crab sizes. The MFS showed a drastic decrease in abundance towards the freshwater discharge, concurrently with decreasing salinities. The triad approach that included water analyses, toxicological experiments and long-term field sampling allowed rejecting relationships between herbicide exposure and mole crab lethal effects.

Assessing the sustainability of buildings using a framework of triad approaches

Five triads are presented that can be used as a framework to rank sustainable measures for the built environment in terms of space, transport, energy, water and materials. These new triads are all based on the same known principle of the Trias Energetica, which constitutes a three-step strategy to come to sustainable energy use. Most favourable measures which prevent energy use are defined as the top step and least favourable measures which only benefit the energy efficiency form the last step. In between, the renewable energy sources have found their position. Analogous to this Trias Energetica, the terms ‘Trias Toponoma’ for space-use, ‘Trias Poreutica’ for transport, ‘Trias Hydrica’ for water consumption and ‘Trias Hylica’ for materials are introduced and explained. The framework of triads can be utilized by city planners, architects, facility managers, house owners, real estate agents and other parties in the building sector to communicate and make decisions on adopting sustainable measures, which will help to decrease the environmental impact of the building sector and to stimulate the development of sustainable buildings.

Theoretical Fluid Mechanics

This special section includes four papers originally presented at the 5th AIAATheoretical Fluid Mechanics Conference (June 2008, Seattle, WA). The papers were included in the Memorial Session for Dr. Norman D. Malmuth, who passed away on 3 July 2007. It is recognized that contemporary problems in fluid mechanics and aerodynamics require an integrated approach that includes fundamental theoretical studies, massive numerical computations, and detailed experimental investigations. This triad approach has been formulated and actively promoted during recent years by the AIAA Fluid Dynamics Technical Committee. Dr. Malmuth was an active member of the Committee, and he strongly supported the concept. His own research was an excellent example of the triad approach. An outline of the role of theoretical studies can be found his lecture [1] presented at the 4th AIAA Theoretical Fluids Mechanics Conference (June 2005, Toronto, Canada). The papers included in the present section serve as a further illustration of how theoretical modeling and insights can be integrated with numerical simulations and experimental studies to significantly improve the understanding of complex problems in modern fluid mechanics. They support Dr. Malmuth’s statement [1]: “Theoretical modeling is an indispensable tool to the engineer and scientist in addition to current emphasis on familiarity and use of legacy codes as well as development of new ones. It still is a very important skill to know how to set up problems from first principles and make approximations as well as combine this capability with modern computational methods.” Anatoli Tumin Zvi Rusak Alexander Fedorov

Comparing different forest zoning options for landscape-scale management of the boreal forest: Possible benefits of the TRIAD

Forest management has been criticised in the last 20 years for its negative impact on the native species, structures and functions of the forest. Of many possible alternatives proposed to minimize these effects, the functional zoning (or TRIAD) approach is gaining popularity in North America. The goal of this approach is to minimize the negative environmental impacts of forestry while maintain timber supply by dividing the forest into three broad land-use zones: (1) conservation, (2) ecosystem management, and (3) wood production. In this study, we used a spatially explicit landscape model to simulate the effects of fire and six different forest management scenarios on a boreal mixedwood forest management unit in central Quebec. The management scenarios examined included the current practices scenario, a scenario proposed by the provincial government, and four TRIAD scenarios varying in the amount of forest allocated to each of the three zones. For each scenario, we examined the harvest volume, percentage old-growth forest or old forest managed to favour old-growth attributes, and effective mesh size of forest patches by 20-year age classes. With more area set aside for conservation and high-retention partial cut harvesting techniques designed to maintain the attributes of old-growth stands, all TRIAD scenarios resulted in higher percentages of stands with old-growth attributes than the current practices scenario and the government proposed scenario, and two of the four TRIAD scenarios also resulted in higher harvest volume over the long term. All forest management scenarios resulted in significantly lower effective mesh size than the fire-only scenario, but this difference was not as pronounced for the four TRIAD scenarios as for the current practice and government proposed scenarios. We conclude that the TRIAD approach has the potential to minimize some of the negative impacts of forestry on the landscape, while maintaining timber supply over the long term.

TRIAD zoning in Quebec: Experiences and results after 5 years

The TRIAD approach to forest management involves dividing the forest into 3 zones, each with its own management objectives, but with the overall goal of increasing the ecological and economic sustainability of the forest. For the past 5 years, we have been experimenting with TRIAD zoning in central Quebec, incorporating social interests into the original concept of TRIAD management. Results generally indicate that this approach is economically viable, socially acceptable, and preferable ecologically in this area. Although much remains to be done, thus far the consensus among the various project participants is that this approach may be a good fit for the public forest of Canada. Key words: TRIAD, functional zoning, sustainable forest management, ecosystem management, natural disturbance based management, intensive forestry, mixed plantations, partial cuts, eco-efficiency, conservation, new forestry, green accounting

Ecological risks of an old wood impregnation mill: application of the Triad approach.

Although many studies deal with the distribution and mobility of chromated copper arsenate (CCA) metals in soil, the ecotoxicity of CCA-contaminated soils is rarely studied. The Triad approach was applied to determine the ecological risks posed by a CCA mixture at a decommissioned wood impregnation mill in southern Finland. A combination of (1) chemical analyses; (2) toxicity tests with plants (aquatic: Lemna minor; terrestrial: Lactuca sativa), earthworms (Lumbricus rubellus), and enchytraeids (Enchytraeus albidus) conducted on contaminated soils, their aqueous extracts, and well water collected from the site; and (3) determination of the abundance of enchytraeids and nematodes and the bioaccumulation of metals in plants (horsetail) collected from the field were used to assess the actual risk. Although metal concentrations were low, L. minor growth appeared to be reduced by As contamination of the well water. In soil, metals were heterogeneously distributed with total concentrations of 14.8 to 4360 mg As/kg, 15.2 to 1740 mg Cr/kg, and 4.83 to 790 mg Cu/kg. In several samples, concentrations were above Finnish regulatory guideline values and exceeded the half maximal effective concentration (EC50) or 50% lethal concentration (LC50) values for the toxicity of the individual metals to earthworms and enchytraeids, indicating hazards to the ecosystem. (Bio)availability of metals was high, as indicated by weak electrolyte extractions and body residues in L. rubellus and E. albidus exposed in bioassays. Earthworm survival correlated significantly with body metal concentrations, but not with soil total metal concentrations. Enchytraeid responses in the soil bioassays were less sensitive to CCA metal exposure. Plant growth was affected by CCA pollution, with L. sativa root elongation correlating significantly with total and available As concentrations and L. minor development being significantly reduced in H2O extracts of the most contaminated soil sample. Abundance of enchytraeids and nematodes in the field was much lower than in nonpolluted Finnish soils but did not significantly correlate with CCA contamination. Arsenic accumulation in horsetail did not correlate with As concentrations in soil. Overall, the results of the 3 lines of evidence of the Triad approach indicate possible increased risks to the ecosystem at the most contaminated sites of the CCA treatment area.

Forest management, restoration, and designer ecosystems: Integrating strategies for a crowded planet

As the global human population increases, the demand to conserve, restore, create, and sustainably manage ecosystems will increase as well. Forested ecosystems are of particular interest because of the biodiversity they support and their diverse values to people. Developments in conservation, restoration forestry, and in the study of designer ecosystems provide a diverse set of tools with which to pursue sustainable forestry goals. Nonetheless, we suggest that sustainable forestry can only be achieved by fully considering ecological, economic, and social needs in landscapes. This will require a clear realization of the trade-offs in site-specific management approaches and a multifaceted, landscape-scale perspective for evaluation of sustainability criteria. We propose collaborative creation of Sustainable Forestry Portfolios as a means to encourage the breadth of thinking required to guide sustainable forest management. We discuss 3 examples of Sustainable Forestry Portfolios with relevance to different settings in the future: 1) the Triad Approach, 2) Forest Landscape Restoration, and 3) Urban Forestry. In all settings, sustainable forestry is not solely a technical problem, but a challenge that must be met through a multidimensional perspective, interdisciplinary collaboration, and with active engagement of the people that live and work in the landscape.

Solutions to the five key brownfield valuation problems

PurposeThe purpose of this study is to solve five key brownfield valuation problems.Design/methodology/approachThis aim is achieved by using doctoral research on integrating the scientific process into the appraisal process. The first objective is demonstrating why four of the problems require solutions prior to solving the first problem, a valuation procedure for formerly used sites. A second objective is to use empirical data from appraisals to reveal why existing methodology is not reliable – because it does not solve the four problems.FindingsThe resulting findings are that a developmental model that incorporates the Triad approach to quantifying environmental uncertainty, initially used in the USA, simulates a process used by buyers to establish the price paid for brownfields with contaminated land.Practical implicationsThe practical implication that results from this research is that valuers need to emulate the buyer's process when valuing this property type. Prescriptive procedures for valuation requiring the use of scientific methods, as used in the Triad process, need to be set forth to quantify the atypical uncertainties in valuing this property type. The results of this research should be of significant interest to all stakeholders that are involved in brownfield redevelopment, so that they can insure that their needs will be met by improved feasibility analysis.Originality/valueThis research is unique in that it is the first empirical test of the reliability of the valuation of brownfields that need to undergo a time‐consuming and often expensive soil remediation process.

Water and sediment quality survey of threatened and endangered freshwater mussel habitat in the Chipola River Basin, Florida

Reduced habitat quality may be contributing to the decline of freshwater mussels in southeastern rivers. As part of an ongoing evaluation of the quality of freshwater mussel habitat in Gulf of Mexico coastal rivers, the Chipola River was assessed during 2006 and 2007. Sediment sam- ples were collected at 8 sites along the Chipola River on May 2 and 3, 2006, and analyzed for conta- minants and tested in the laboratory for toxicity to Hyalella azteca using 29 d exposures to solid- phase sediment and 96 h exposures to sediment porewater. In addition, benthic macroinvertebrate populations were sampled at each site. Using the sediment quality triad approach (chemistry, toxic- ity and in situ benthic assemblages) to assess habitat quality, 3 sites (1, 4 and 8) were shown to be impaired, with Site 4 being the most impaired site. H. azteca survival in porewater exposures was reduced, and trace elements were elevated in sediments at these sites; however, concentrations were not considered exceedingly high. Water quality samples collected did not violate the State of Florida's water quality standards. The lack of concordance among the test metrics (in situ benthic assemblages were not impaired) at these sites suggests marginal habitat impairment.

Application of a new sediment contact test with Myriophyllum aquaticum and of the aquatic Lemna test to assess the sediment quality of Lake Skadar

Situated in the transboundary belt between Montenegro and Albania, Lake Skadar is the largest freshwater reservoir in Southeastern Europe. Because of the wide range of endemic, rare or endangered plant and animal species it supports, Lake Skadar and its extensive adjacent wetlands are internationally recognised as a site of significance and importance (Ramsar site). Within the last 10 to 20 years, Lake Skadar was exposed to intensive pollution. For the assessment of the ecotoxic load of the sediments sampled in Lake Skadar, a triad approach was recently applied. Overall, a complex spectrum of ecotoxic loads was elucidated. The aim of the present study was to use plant-based bioassays for assessing the sediment quality of Lake Skadar in order to facilitate and complement the triad test battery. The newly developed sediment contact test with Myriophyllum aquaticum and the aquatic growth inhibition test with Lemna minor were applied to native sediments and pore water, respectively, allowing the investigation of different toxicity-effects caused by particle-bound pollutants as well as pollutants in the interstitial water. This investigation is the first application of the novel sediment contact test with Myriophyllum aquaticum to lake sediments. Sediment samples were taken from nine selected sites at Lake Skadar and investigated by the sediment contact assay with Myriophyllum aquaticum. The pore water was extracted from these sediment samples to be analysed in the aquatic growth inhibition test with Lemna minor. The results of the sediment contact tests were compared with each other and with those of the aquatic growth inhibition test. Both applied macrophyte biotests revealed distinct changes in the growth behaviour of the two macrophytes subsequent to the exposure to the investigated natural sediments of Lake Skadar. The Myriophyllum sediment contact test revealed significant toxicity in the sediment samples from Radus and Kamenik, whereas the aquatic Lemna test showed inhibition effects for the samples from Sterbeq, Plavnica and Kamice. Data obtained with the newly developed Danio rerio contact test and the Arthrobacter globiformis contact test confirmed the Myriophyllum results. Analyses of the heavy metal content in the sediments revealed low or moderate contamination levels. Correlation analyses between the content of heavy metals in the sediments and growth inhibition of Myriophyllum aquaticum showed a significant correlation between Cr concentrations and growth inhibition. Comparable findings are available for a German river system. In contrast, no significant correlation between inhibition rates and concentration of metals could be observed with Lemna minor. It was shown that the newly developed sediment contact test with Myriophyllum aquaticum is applicable to lake sediments. In both the sediment contact test with Myriophyllum aquaticum on whole sediments and the aquatic growth inhibition test with Lemna minor on pore water, plant growth was influenced by the natural sediments and its components. Therefore, both test systems were found to be suitable for the detection of phytotoxic effects upon exposure to sediments. Myriophyllum aquaticum as test organism of the contact test grows directly in the sediment without an additional water-layer. Thus, it is able to detect toxicity caused by particle-bound phytotoxic substances as well as pore water-related contamination, while the floating Lemna minor can only detect effects emanating from pore water. Significant differences of the results were observed between these two test systems and, accordingly, the two different exposure scenarios. Hence, none of the tests can replace the other one and, as a consequence, both should be included into a test battery for the assessment of sediment toxicity. Both plant assays were shown to be reliable tools for the evaluation of the eco-toxicological risk potentials of pore water and solid-phase sediment. They should become a complement to the standardised test battery generally used for comprehensive hazard assessment.

Integration of bioavailability, ecology and ecotoxicology by three lines of evidence into ecological risk indexes for contaminated soil assessment

A Weight of Evidence approach was applied to define three integrated effect indexes estimating the impairment on terrestrial ecosystems caused by the stressor(s) of concern. According to a Triad approach, the integrated effect indexes combined the information provided by the measurement endpoints of each line of evidence (chemistry/bioavailability, ecology and ecotoxicology) and allowed to analyse the impairment degree highlighted by each measurement endpoint as difference from the reference condition. Multi-Criteria Decision Analysis (MCDA) was used for the aggregation of the complementary Triad information, including expert judgement and a weighted procedure based on the endpoint sensitivity and the sensitivity of the test for ecosystem effects. The developed methodology was implemented in the DSS-ERAMANIA, Module 2, and is presented in this paper as “Integrated Effect Indexes” (IEI) sub-module. The latter has been preliminary applied to the Acna di Cengio (Italy) contaminated site; the results of this application are presented and discussed.

Environmental Toxicity Testing in the Risk Assessment of a Metal Contaminated Abandoned Mining Site in Hungary

A three tiered, iterative Environmental Risk Assessment methodology, including preliminary Qualitative Risk Assessment, Quantitative Hazard Assessment and Site Specific Quantitative Risk Assessment, was established to assess the environmental risk of point and diffuse pollution of mining origin at catchment scale [1]. The model site was an abandoned Pb and Zn sulphide ore mine in Gyöngyösoroszi, Toka-valley, NE Hungary [2]. The Integrated Risk Model considers the sources identified by the GIS-based (Geographical Information System) pollution map, the transport routes shown by the GIS-based flow accumulation model and the receptors of different land uses in the catchment. The site-specific quantitative risk was characterised by the Soil Testing Triad [3]. The three elements of the Triad are: physico-chemical analyses of the soil and the contaminants, the biological characterisation and ecotoxicity testing of the contaminated soil, measuring the response of single species in laboratory bioassays, the natural response of the soil microflora and plants or the dynamic response of the whole soil in microcosms. The Triad approach strongly supports the characterisation of the site specific risk as well as the selection and planning of the suitable remediation option.

Using a Sediment Quality Triad Approach to Evaluate Benthic Toxicity in the Lower Hackensack River, New Jersey

A Sediment Quality Triad (SQT) study consisting of chemical characterization in sediment, sediment toxicity and bioaccumulation testing, and benthic community assessments was performed in the Lower Hackensack River, New Jersey. Chemistry data in sediment and porewater were evaluated based on the equilibrium partitioning approach and other published information to investigate the potential for chemical effects on benthic organisms and communities. Relationships were supported by laboratory toxicity and bioaccumulation experiments to characterize chemical effects and bioavailability. Benthic community results were evaluated using a regional, multimetric benthic index of biotic integrity and four heterogeneity indices. Evidence of slight benthic community impairment was observed in five of nine sediment sample stations. Severe lethal toxicity to amphipods (Leptocheirus plumulosus) occurred in four of these five stations. Although elevated total chromium concentrations in sediment (as high as 1900 mg/kg) were the rationale for conducting the investigation, toxicity was strongly associated with concentrations of polycyclic aromatic hydrocarbons (PAHs) rather than total chromium. PAH toxic units (SigmaPAH TU) in sediment and SigmaPAH concentrations in laboratory organisms from the bioaccumulation experiment showed a clear dose-response relationship with toxicity, with 0% survival observed in sediments in which SigmaPAH TU > 1-2 and SigmaPAH concentrations in Macoma nasuta were >2 micromol/g, lipid weight. Metals detected in sediment and porewater, with the possible exception of copper, did not correlate with either toxicity or levels in tissue, likely because acid-volatile sulfide levels exceeded concentrations of simultaneous extracted metals at all sample locations. The study reinforces the value of using multiple lines of evidence approaches such as the SQT and the importance of augmenting chemical and biological analyses with modeling and/or other approaches to evaluate chemical bioavailability and toxicity of sediments.

Validation of a FFQ to estimate the intake of PUFA using plasma phospholipid fatty acids and weighed foods records

Due to the growing knowledge about the role of specific fatty acids in health and disease, dietary intake measurements of individual fatty acids or classes of fatty acids are becoming increasingly important. The objective of this study was to evaluate the ability of the Nambour FFQ to estimate intakes of specific fatty acids, particularly PUFA. The study population was a sub-sample of adult participants in a randomised controlled trial of beta-carotene and sunscreen in the prevention of skin cancer (n 43). Dietary intake was assessed by a self-administered FFQ and a weighed food record (WFR). Non-fasting blood samples were collected and analysed for plasma phospholipid fatty acids. Median intakes on the FFQ were generally higher than the WFR except for the n-3 PUFA groups, where the FFQ estimated higher intakes. Correlations between the FFQ and WFR were moderate (r 0 x 32-0 x 59) except for trans fatty acids (r 0 x 03). Correlations between each of the dietary assessment methods and the plasma phospholipids were poor for all fatty acids other than the PUFA. Using the methods of triads approach, the FFQ validity coefficients for total n-3 fatty acids, total long chain n-3 fatty acids, EPA, arachidonic acid, docosapentaenoic acid and DHA were 0 x 50, 0 x 63, 0 x 45 and 0 x 62 and 0 x 62, respectively. For most fatty acids, the FFQ adequately estimates group mean fatty acid intakes and can adequately rank individuals; however, the ability of this FFQ to estimate trans fatty acids was poor.