Tree Trunks Research Articles

Host specificity of two co‐occurring nesting‐forms of the bag‐shelter moth Ochrogaster lunifer (Lepidoptera: Notodontidae) is congruent with there being cryptic species

AbstractThe Bag‐shelter moth, Ochrogaster lunifer (Lepidoptera: Notodontidae), is an Australian endemic species of Thaumetopoeinae with a univoltine life cycle and gregarious, herbivorous larvae. It is variable throughout its range across the continent, most noticeably by the species of host tree used and nest‐building behaviour. It has long been considered a species complex by some taxonomists, and the lack of gene flow between populations of ground‐nesters and above‐ground nesters at the same sites provides strong evidence for at least two species. We tested the specificity of host use and nesting behaviour of ground‐nesting and trunk‐nesting forms of O. lunifer by transplanting field‐collected egg masses to the other form's host, either in their natural position or in the position used by the other form. At the study site, ground‐nesting and trunk‐nesting (tree‐hugger) O. lunifer coexist. Ground‐nesting larvae are found on Acacia spp., and egg masses are laid at the base of host tree trunks where nests develop whereas tree‐hugger larvae are found on Blakella tessellaris, egg masses are laid in the outer canopy, and nests develop on the trunk or large branches. Of the 47 egg masses of O. lunifer transplanted to the unnatural host, only one cohort of tree‐hugger larvae developed through to adult moths. Placing an egg mass in its unnatural position on its natural host did not prevent the larvae from developing and successfully establishing nests for either nesting‐form, although all the ground‐nesters moved position (from the canopy to the ground) and the tree‐huggers mostly did not (from the ground to the canopy). Only the tree‐hugger form of O. lunifer developed a viable nest on the unnatural host species, established a nest at the unnatural oviposition site, and shared that nest with larvae of the other nesting‐form, suggesting tree‐hugger larvae are somewhat plastic in their behaviour and ecology. Our findings add to previous studies indicating that Acacia is likely the ancestral host of O. lunifer though large areas of its range remain unstudied.

Synergizing a Deep Learning and Enhanced Graph-Partitioning Algorithm for Accurate Individual Rubber Tree-Crown Segmentation from Unmanned Aerial Vehicle Light-Detection and Ranging Data

The precise acquisition of phenotypic parameters for individual trees in plantation forests is important for forest management and resource exploration. The use of Light-Detection and Ranging (LiDAR) technology mounted on Unmanned Aerial Vehicles (UAVs) has become a critical method for forest resource monitoring. Achieving the accurate segmentation of individual tree crowns (ITCs) from UAV LiDAR data remains a significant technical challenge, especially in broad-leaved plantations such as rubber plantations. In this study, we designed an individual tree segmentation framework applicable to dense rubber plantations with complex canopy structures. First, the feature extraction module of PointNet++ was enhanced to precisely extract understory branches. Then, a graph-based segmentation algorithm focusing on the extracted branch and trunk points was designed to segment the point cloud of the rubber plantation. During the segmentation process, a directed acyclic graph is constructed using components generated through grey image clustering in the forest. The edge weights in this graph are determined according to scores calculated using the topologies and heights of the components. Subsequently, ITC segmentation is performed by trimming the edges of the graph to obtain multiple subgraphs representing individual trees. Four different plots were selected to validate the effectiveness of our method, and the widths obtained from our segmented ITCs were compared with the field measurement. As results, the improved PointNet++ achieved an average recall of 94.6% for tree trunk detection, along with an average precision of 96.2%. The accuracy of tree-crown segmentation in the four plots achieved maximal and minimal R2 values of 98.2% and 92.5%, respectively. Further comparative analysis revealed that our method outperforms traditional methods in terms of segmentation accuracy, even in rubber plantations characterized by dense canopies with indistinct boundaries. Thus, our algorithm exhibits great potential for the accurate segmentation of rubber trees, facilitating the acquisition of structural information critical to rubber plantation management.

Soil chemical responses to fertilization, with or without a cover crop, in an olive orchard in southwestern Buenos Aires (Argentina)

Our objective was to study the effect of fertilization on soil chemical traits on an olive orchard (artificially irrigated), considering areas with or without a cover crop, in southwestern Buenos Aires, Argentina, during the period 2020/2021. Fertilization treatments were (1) organic manure applied to the soil near the tree trunk; inorganic fertilization applied to the (2) soil or (3) to the leaves of Olea europaea L. trees; and (4) unfertilized control. Seeding of Vicia benghalensis L. and Avena sativa L. around subplots (one per each of the four studied treatments) constituted the areas with a cover crop. Subplots which were not seeded corresponded to the control areas. Soil pH was lower (p<0.05) under organic and inorganic soil fertilization. In areas without a cover crop in April 2021, soil nitrate concentrations were greater (p<0.05) under organic soil fertilization than in the control. At 0-20 cm soil depth, P concentrations were greater (p<0.05) under organic and inorganic soil fertilizations than in the other treatments. The greatest (p<0.05) K concentrations were found in the organic fertilization treatment. Organic soil fertilization on areas without a cover crop showed greater values for the soil chemical studied traits.

Effects of fertile islands on soil organic carbon density and labile organic carbon distribution in elm (Ulmus macrocarpa Hance)-dominated sparse wood grasslands in southeastern Inner Mongolia, China

In semi-arid savanna grasslands, the sparse distribution of trees often forms what are commonly known as fertile islands. However, the effect of these trees on soil organic carbon (SOC), microbial biomass carbon (MBC), and dissolved organic carbon (DOC), remains poorly understood. In this study, the spatial distribution patterns of SOC, MBC, and DOC were investigated across soil profiles (0–5, 5–10, 10–30, 30–50, and 50–100 cm) at three distances from the tree trunk in an elm-dominated savanna ecosystem in Northeast China. Our results suggest that SOC, MBC, and DOC were significantly influenced by both the distance from the tree and the soil depth. The research provides evidence that elm trees can form fertile island effects, thereby enhancing the contents of SOC and labile organic carbon fractions.

THE ISSUES OF MEDIEVAL RITUAL OAKS IN THE MIDDLE AND UPPER DNIEPER BASIN

In 2023 the abstracts of N. Khamayko, O. Pashkovsky, P. Goldin, M. Kubliya, and E. Yanish «Scientific studies of the cult “Oak of the Slavs» were published. The publication is focused on three pagan ritual oaks that were discovered in the Dnieper and Desna interfluve in 1909, 1975 and 1978/1979. The mandibles of pigs were embedded into these trees. The last two oaks, according to 14C indicators, are dated within the third quarter / late 7th — late 10th / mid-11th centuries. The main part of the mentioned publication is the results of laboratory archaeozoological research of animal remains from an oak trunk raised from the bottom of the Dnipro in 1975 conducted by employees of the Institute of Zoology of the National Academy of Sciences of Ukraine in 2020 and 2023. The researches have shown that the bones belong mainly to domestic or hybrid (semi-domestic/semi-wild) sows; the age of most animals is up to 3 years or more; the time of death of three animals falls on the second half of the annual cycle, and in two cases the period of death is determined by the winter season. According to most laboratory indicators, the bone remains of animals from the oak of 1975 were similar or identical to the corresponding indicators of the pig mandibles from the oaks of 1978/1979 maintained by the Institute of Zoology of the National Academy of Sciences of Ukraine in 1987. This proves the similarity between the analyzed specimens not only at the level of the main zoological and biological components. One can see the similarity or identity at the level of the content of the pagan ritual, during which the jaws of sows were inserted into tree trunks. The main indicators of archaeozoological and dendrological (regarding the oak in 1978/1979) research, as well as historical and ethnographic research, convince that the mentioned ritual trees are the realities of the calendar rituals of the autumn and winter seasons (in particular, perhaps, the rituals of productive magic) of local agrarians, and not the rituals of traveling merchants-wives, as was considered until recently. In the mentioned publication, the authors also touched upon the question of the ethnocultural attribution of the analyzed ritual oaks. Having based on a number of facts mainly of a general historical nature, in particular: 1) the appearance of the Vikings on the lands of the Lower Desna — the Middle Dnieper in the period of early Rus’; 2) the spread of the cult of trees and animals among pagan Scandinavians; 3) calibrated dating range of ritual oaks in 1975 and 1978/1979 within 776—938 years, i.e. largely within the Early Rus area; 4) change in the 10th century Slavic archaeological cultures of the Middle Dnieper region to the archaeological culture of Old Rus, they put in doubt (although not directly) the probable belonging of these trees to the local Slavs. However, this attempt lacks concrete and therefore convincing evidence of a historical, archaeological or historical-ethnographic nature. There is no reason to assume a significant influence of the Scandinavian newcomers on the spiritual sphere of the autochthons, especially on their calendar rituals of the agricultural cycle.

Epiphytic Patterns Impacting Metabolite Diversity of Drynaria roosii Rhizomes Based on Widely Targeted Metabolomics.

Drynaria roosii Nakaike, a fern widely distributed in China and some countries in Southeast Asia, is a commonly used herbal medicine in tonic diets and Chinese patented medicine. The metabolites of its dried rhizomes are easily affected by the epiphytic pattern, whether on rock tunnels (RTs) or tree trunks (TTs). The current research focused on rhizomes from these two patterns, RTs and TTs (further divided into subclasses TA, TB, TC, and TD, based on trunk differences) and conducted a widely targeted metabolomics analysis. A total of 1435 components were identified across 13 categories, with flavonoids, amino acids, and their derivative, lipids, identified as the main components. They accounted for 19.96%, 12.07%, and 12.14% of all metabolites, respectively. The top five flavonoids in TB were eriodicty-ol-7-O-(6″-acetyl)glucoside, quercetin-3-O-sophoroside (baimaside), dihydrochar-cone-4'-O-glucoside, morin, and hesperetin-7-O-glucoside, with relative contents 76.10, 24.20, 17.02, 15.84, and 14.64 times higher than in RTs. Principal component analysis revealed that samples with different epiphytic patterns clustered into five groups. The RT patterns revealed unique metabolites that were not detected in the other four epiphytic species (TA, TB, TC, and TD), including 16 authenticated metabolites: 1 alkaloid, 1 amino acid derivative, 7 flavonoids, 2 lignans, 1 lipid, 1 alcohol, 1 aldehyde, and 2 phenolic acids. These differences in epiphytic patterns considerably affected the accumulation of both primary and secondary metabolites. The comparison of diversity between RTs and TTs can guide the selection of a cultivation substance and the grading of collective rhizomes in the wild. This comprehensive analysis of D. roosii rhizome metabolites also offers fundamental insights for identifying active components and understanding the mechanisms underlying their potential pharmacological activities.

Ubiquity of methanogenic archaea in the trunk of coniferous and broadleaved tree species in a mountain forest.

Wetwood of living trees is a habitat of methanogenic archaea, but the ubiquity of methanogenic archaea in the trunk of various trees has not been revealed. The present study analysed methanogenic archaeal communities inside coniferous and broadleaved trees in a cold temperate mountain forest by culture-dependent or independent techniques. Heartwood and sapwood segments were obtained from the trunk of seven tree species, Cryptomeria japonica, Quercus crispula, Fraxinus mandshurica, Acer pictum, Aesculus turbinata, Magnolia obovata, and Populus tremula. Amplicon sequencing analysis of 16S rRNA genes showed that Methanobacteriaceae predominated the archaeal communities and Methanomassiliicoccaceae also inhabited some trees. Real-time PCR analysis detected methanogenic archaeal mcrA genes from all the tree species, with a maximum of 107copiesg-1 dry wood. Digital PCR analysis also detected mcrA genes derived from Methanobacterium spp. and Methanobrevibacter spp. from several samples, with a maximum of 105 and 104copiesg-1 dry wood. The enumeration by the most probable number method demonstrated the inhabitation of viable methanogenic archaea inside the trees; 106cellsg-1 dry wood was enumerated from a heartwood sample of C. japonica. Methanogenic archaea related to Methanobacterium beijingense were cultivated from a heartwood sample of Q. crispula and F. mandshurica. The present study demonstrated that the inside of various trees is a common habitat for methanogenic archaeal communities and a potential source of methane in forest ecosystems.

Tree-Climbing Behavior of a Forest-Dwelling Ungulate: The Formosan Serow.

Ungulates are terrestrial herbivores, basically adapted to running fast on the ground; tree-climbing behavior has been reported only in seven species, and five of them live in open habitats (Capra hircus, C. aegagrus, C. falconeri, C. cylindricornis, Oreotragus oreotragus). Tree-climbing behavior may also be evolved in ungulates inhabiting dense forests with abundant trees; however, this has rarely been reported in such species (Moschus leucogaster, M. moschiferus), probably due to the difficulty of observing in the wild. The numerous publicly available records in social networks hold potentially valuable information on the atypical behaviors of wild ungulates. Here, we explored the tree-climbing behavior of a forest-dwelling ungulate, the Formosan serow in Taiwan, a subtropical island, by extracting information from online social media platforms. We researched images and videos of Formosan serows through Facebook and YouTube and collected a total of 15 tree-climbing events. In these materials, Formosan serows climbed 10 tree species, including evergreen coniferous and broad-leaved trees, and a variety of parts, ranging in height from 0.6 to 4 m, and from branches of shrubs to trunks of tall trees. Tree-climbing behavior was recorded throughout Taiwan and from lowlands to subalpine zones, suggesting that tree climbing may be a common behavior in this species. Foraging while climbing trees was frequently observed (53.3%), suggesting that the purpose or benefit for climbing is to obtain additional food other than plants growing near the ground surface. In contrast to other tree-climbing ungulates, Formosan serows climbed trees not only in winter, but also in other seasons, when food is relatively abundant. This is the first scientific report of tree-climbing behavior in the Formosan serow that is typically a forest dweller.

Fungal-plant interaction: a pathogenic relationship between Ganoderma segmentatum sp. nov. and Vachellia nilotica.

The diversity of Ganoderma remains largely unexplored, with little information available due to fungiphobia and the morphological plasticity of the genus. To address this gap, an ongoing study aims to collect and identify species with this genus using nuclear ribosomal DNA regions called the "Internal Transcribed Spacer" (ITS1-5.8S-ITS2 = ITS). In this study, a new species, Ganoderma segmentatum sp. nov., was discovered on the dead tree trunk of the medicinal plant, Vachellia nilotica. The species was identified through a combination of morpho-anatomical characteristics and phylogenetic analyses. This new species was closely related to Ganoderma multipileum, G. mizoramense, and G. steyaertanum, with a 99% bootstrap value, forming a distinct branch in the phylogenetic tree. Morphologically, G. segmentatum can be distinguished by its frill-like appearance on the margin of basidiome. Wilt or basal stem rot, a serious disease of trees caused by Ganoderma species and V. nilotica, is brutally affected by this disease, resulting in substantial losses in health and productivity. This Ganoderma species severely damages V. nilotica through deep mycelial penetration in the upper and basal stems of the host species. Pathogenic observational descriptions of G. segmentatum on dead tree trunks showed the exudation of viscous reddish-brown fluid from the basal stem portion, which gradually extended upward. Symptoms of this disease include decay, stem discoloration, leaf drooping, and eventual death, which severely damaged the medicinal tree of V. nilotica.

Impact of Wood Structure Variability on the Surface Roughness of Chestnut Wood

Wood constitutes a unique and valuable material that has been used from ancient times until nowadays in a wide variety of applications, in which the surface quality of wood often constitutes a critical factor. In this study, the influence of different wood areas and therefore, of different anatomical characteristic areas of chestnut wood (Castanea sativa Mill.) on the surface quality, was thoroughly studied, in terms of surface roughness. Five different chestnut tree trunks were harvested, from which five different disks were obtained corresponding to five different trunk heights. Surface roughness was measured on these disks on the transverse, radial, and tangential planes, on the areas of sapwood and heartwood, measuring the roughness in each point both vertically and in parallel to the wood grain. The results revealed that the examined roughness indexes (Ra, Rz, Rq) follow a parallel path to one another. In the case of all surfaces (transverse, radial, tangential) of the disks examined, when the measurement was implemented perpendicularly to the wood grain, a significantly higher roughness was recorded, compared to the wood grain measurements being implemented in parallel with the wood grain. Significant differences between heartwood and sapwood roughness were not demonstrated, although sapwood often appeared to exhibit a higher surface roughness than heartwood sites. Among the roughness values of the three different surfaces, the highest roughness in the vertical-to-wood-grain measurements was recorded by tangential surfaces, with slightly lower values on the transverse surfaces and the lowest roughness on radial surfaces. Meanwhile, for the measurements in parallel with the wood grain, the transverse surfaces presented significantly higher roughness values compared to the tangential and radial surfaces. Significant roughness differences were not detected among the surfaces at different trunk heights. Although, significant differences in roughness were recorded among different trees, it was observed that all the studied trees align with the identified and described within-tree trends.

Soil Physicochemical Properties under Selected Avocado Cultivars in Ethiopian Smallholder Agroforestry

This study investigated the impact of three avocado cultivars on selected soil physicochemical properties in Central Ethiopia, to enhance the knowledge on the influence of avocado cultivars on soil physicochemical properties, and assist smallholders in cultivar selection in agroforestry. Trees planted in farmers´ fields 8 years earlier were revisited. Soil samples were collected from 0–20 cm and 20–40 cm depth at three radial distances from trees (1 m and 2 m from tree trunk, and at 5 m from the canopy edge as a control). Soil texture, bulk density, moisture content, pH, electrical conductivity, soil organic carbon, total nitrogen and available phosphorus were determined. Soil moisture content and electrical conductivity were overall higher and bulk density was lower (p < 0.05) under the canopies of the avocado trees than in the control and soil organic carbon, total nitrogen and available phosphorus tended to increase in spite of nutrient inputs to the control whilst the trees were unfertilized. Differences between the studied cultivars were small, but Hass tended to have the largest impact on soil nutrient levels, whilst Ettinger and Nabal tended to have a somewhat larger effect on the soil organic carbon concentration. Integrating these avocado cultivars on farms can improve soil fertility in the study area. However, for optimal agricultural soil health and sustainable avocado production, cultivar and site-specific soil management practices must be applied.

Comparing YOLOv8 and Mask R-CNN for instance segmentation in complex orchard environments

Instance segmentation, an important image processing operation for automation in agriculture, is used to precisely delineate individual objects of interest within images, which provides foundational information for various automated or robotic tasks such as selective harvesting and precision pruning. This study compares the one-stage YOLOv8 and the two-stage Mask R-CNN machine learning models for instance segmentation under varying orchard conditions across two datasets. Dataset 1, collected in dormant season, includes images of dormant apple trees, which were used to train multi-object segmentation models delineating tree branches and trunks. Dataset 2, collected in the early growing season, includes images of apple tree canopies with green foliage and immature (green) apples (also called fruitlet), which were used to train single-object segmentation models delineating only immature green apples. The results showed that YOLOv8 performed better than Mask R-CNN, achieving good precision and near-perfect recall across both datasets at a confidence threshold of 0.5. Specifically, for Dataset 1, YOLOv8 achieved a precision of 0.90 and a recall of 0.95 for all classes. In comparison, Mask R-CNN demonstrated a precision of 0.81 and a recall of 0.81 for the same dataset. With Dataset 2, YOLOv8 achieved a precision of 0.93 and a recall of 0.97. Mask R-CNN, in this single-class scenario, achieved a precision of 0.85 and a recall of 0.88. Additionally, the inference times for YOLOv8 were 10.9 ms for multi-class segmentation (Dataset 1) and 7.8 ms for single-class segmentation (Dataset 2), compared to 15.6 ms and 12.8 ms achieved by Mask R-CNN's, respectively. These findings show YOLOv8's superior accuracy and efficiency in machine learning applications compared to two-stage models, specifically Mask-R-CNN, which suggests its suitability in developing smart and automated orchard operations, particularly when real-time applications are necessary in such cases as robotic harvesting and robotic immature green fruit thinning.

Биологическая эффективность способа инъекции гербицидов и их смесей в стволы деревьев нежелательных пород

Целью работы являлось совершенствование регулирования состава и густоты древостоев способом инъекции гербицидов и их смесей в стволы деревьев лиственных пород для повышения лесоводственной эффективности, снижения химической и токсической нагрузки на лесные экосистемы. Объектами исследований являлись отдельные деревья ольхи серой и осины вегетативного происхождения в фазе жердняка в смешанных насаждениях. В результате анализа отечественной и зарубежной литературы по проблеме были отобраны перспективные для изучения гербициды – торнадо (водный раствор, 360 г/л глифосата в виде изопропиламинной соли) и арбонал (водорастворимый концентрат, 250 г/л имазапира) и заложены полевые опыты с отобранными гербицидами. В четырех полевых опытах определялась биологическая эффективность действия гербицидов и их смесей на деревья ольхи серой и осины в смешанных насаждениях. В результате установлена высокая эффективность действия гербицида арбонал в дозах 0,08 и 0,16 мл/дерево, а также смесей торнадо (0,12–0,25 мл/дерево) + арбонал (0,06–0,08 мл/дерево) на ольху серую. По действию на осину наиболее эффективным было применение торнадо 0,20 и 0,55 мл/дерево, арбонала (0,16 мл/дерево) и смесей торнадо (0,20 мл/дерево) + арбонал (0,08 мл/дерево) и торнадо (0,25 мл/дерево) + арбонал (0,06 мл/дерево). Ольха серая проявила большую устойчивость к примененным гербицидам в год обработки, однако следует ожидать усиления действия гербицидов в следующем вегетационном сезоне. Установлено, что применение гербицидов торнадо и арбонал, а также их смесей в различных сочетаниях весьма перспективно для подавления нежелательной древесной растительности способом инъекции в смешанных насаждениях. The aim of the work was to improve the regulation of the composition and density of forest stands by injecting herbicides and their mixtures into the trunks of hardwood trees to increase silvicultural efficiency, reduce the chemical and toxic load on forest ecosystems. The objects of research were individual trees of gray alder and aspen of vegetative origin in the pole stage in mixed stands. As a result of the analysis of domestic and foreign literature on the problem, promising herbicides for study were selected – tornado (aqueous solution, 360 g/l glyphosate in the form of isopropylamine salt) and arbonal (water-soluble concentrate, 250 g/l imazapyr) and field experiments were laid with selected herbicides. In four field experiments, the biological effectiveness of the action of herbicides and their mixtures on gray alder and aspen trees in mixed plantations was determined. As a result, the high efficiency of the herbicide arbonal at doses of 0.08 and 0.16 ml/tree, as well as mixtures of tornado (0.12–0.25 ml/tree) + arbonal (0.06–0.08 ml/tree) was established on gray alder. According to the effect on aspen, the most effective was the use of tornado 0.20 ml/tree and 0.55 ml/tree), arbonal (0.16 ml/tree) and mixtures of tornado (0.20 ml/tree) + arbonal (0.08 ml/tree) and tornado (0.25 ml/tree) + arbonal (0.06 ml/tree). Alder gray showed greater resistance to the applied herbicides in the year of treatment, but we should expect an increase in the effect of herbicides in the next growing season. It has been established that the use of herbicides tornado and arbonal, as well as their mixtures in various combinations, is very promising for the suppression of unwanted woody vegetation by injection in mixed stands.

Crash-perching on vertical poles with a hugging-wing robot

Perching with winged Unmanned Aerial Vehicles has often been solved by means of complex control or intricate appendages. Here, we present a method that relies on passive wing morphing for crash-landing on trees and other types of vertical poles. Inspired by the adaptability of animals’ and bats’ limbs in gripping and holding onto trees, we design dual-purpose wings that enable both aerial gliding and perching on poles. With an upturned nose design, the robot can passively reorient from horizontal flight to vertical upon a head-on crash with a pole, followed by hugging with its wings to perch. We characterize the performance of reorientation and perching in terms of impact speed and angle, pole material, and size. The robot robustly reorients at impact angles above 15° and speeds of 3 m ⋅ s−1 to 9 m ⋅ s−1, and can hold onto various pole types larger than 28% of its wingspan in diameter. We demonstrate crash-perching on tree trunks with an overall success rate of 73%. The method opens up new possibilities for the use of aerial robots in applications such as inspection, maintenance, and biodiversity conservation.

Time Optimization Analysis Of Drainage Projects (Case Study: Surya Indah II Complex Drainage Construction)

The rapid development of the world of construction means that competition in the construction services business must be able to compete. Therefore, careful consideration is required to choose a method so that a construction project can run effectively and efficiently with results that meet the objectives, one of which is the Surya Indah II Complex Drainage Construction. In a development project, considerable processing is required, taking into account the increasing size of the project and the complexity of the dependencies between one part of the work and another in a project to achieve the desired results. Proper scheduling along with adequate resource allocation contributes to project success. This is comparable to the Surya Indah II Complex Drainage Development Project in Banjarbaru City, which has a contract value of 1,480,920,041 billion. This project was supposed to be completed in 180 calendar days; however, it was delayed due to a variety of issues, including bad weather, a shortage of labor, and tardy mobilization. According to the data analyzed in this study, the CPM (critical path method) approach has a critical route. The total project period using the CPM approach is 170 days, calculated based on the longest critical route. Work networks studied using the critical path include mobilization work, tree trunk cutting, concrete demolition, asphalt cutting, box culverts, and other tasks. Using the CPM approach, you can cut the job duration by 2.8% of the contract value. The solution to speed up the duration obtained using the CPM method is to add workers, add productivity tools, add working hours to activities that are on the critical path, and add the Earned Value method for optimal results.

A morphological observation on the Cenozoic beetle Elektrocateres rappsilberi gen. et sp. nov. (Lophocateridae) using digital microscopy and synchrotron X-ray microtomography

ABSTRACT Elektrocateres rappsilberi gen. et sp. nov. is described from Bitterfeld or Saxonian amber. It is the first representative of the superfamily Cleroidea (Coleoptera) recorded in this amber, from which only 10 families of beetles were known until now. The new genus and species was studied using classical stereomicroscopy, as well as digital microscopy and synchrotron-radiation-based micro-computed tomography. The fossil is related to the rare extant monotypic genera Trichocateres Kolibáč, 2010 and Carinicateres Kolibáč, 2021 from tropical forests of Southeastern Asia. It is assumed that Elektrocateres rappsilberi gen. et sp. nov. would be predatory, hunting other arthropods on tree branches and trunks; however, its mouthparts are probably still on halfway between herbivory or fungivory and predatory.

Trunk distortion weakens the tree productivity revealed by half-sib progeny determination of Pinus yunnanensis

Twisted trunks are not uncommon in trees, but their effects on tree growth are still unclear. Among coniferous tree species, the phenomenon of trunk distortion is more prominent in Pinus yunnanensis. To expand the germplasm of genetic resources, we selected families with excellent phenotypic traits to provide material for advanced generation breeding. The progeny test containing 93 superior families (3240 trees) was used as the research material. Phenotypic measurements and estimated genetic parameters (family heritability, realistic gain and genetic gain) were performed at 9, 15, and 18 years of age, respectively. The genetic evaluation yielded the following results (1) The intra-family variance component of plant height (PH) was greater than that of the inter-family, while the inter-family variance components of other traits (diameter at breast height (DBH), crown diameter (CD), height under branches (HUB), degree of stem-straightness (DS)) were greater than that of the intra-family, indicating that there was abundant variation among families and potential for selection. (2) At half rotation period (18 years old), there was a significant correlation among the traits. The proportion of trees with twisted trunks (level 1–3 straightness) reached 48%. The DS significantly affected growth traits, among which PH and DBH were the most affected. The volume loss rate caused by twisted trunk was 18.06-56.75%, implying that trunk distortion could not be completely eliminated after an artificial selection. (3) The influence of tree shape, crown width, and trunk on volume increased, and the early-late correlation between PH, DBH and volume was extremely significant. The range of phenotypic coefficient of variation, genetic variation coefficient and family heritability of growth traits (PH, DBH, and volume) were 44.29-127.13%, 22.88-60.87%, and 0.79–0.83, respectively. (4) A total of 21 superior families were selected by the method of membership function combined with independent selection. Compared with the mid-term selection (18 years old), the accuracy of early selection (9 years old) reached 77.5%. The selected families’ genetic gain and realistic gain range were 5.79-19.82% and 7.12-24.27%, respectively. This study can provide some useful reference for the breeding of coniferous species.

ANT SPECIES DIVERSITY (HYMENOPTERA, FORMICIDAE) IN THE PURWODADI BOTANICAL GARDENS, EAST JAVA, INDONESIA

The ant species diversity in tropical Asia has been investigated in several locations, however, these studies mainly focused on well preserved forests or plantations. The ant fauna in urban parks has only been investigated in a limited number of locations in Singapore, Hong Kong, Macao, and Indonesia. We investigated the ant fauna in the Purwodadi Botanical Gardens, East Java, and compared it to the ant fauna obtained in the Bogor Botanical Gardens. In the Purwodadi Botanical Gardens, we employed the following sampling methods that were also carried out in Bogor: (1) visual searching of ants on tree trunks, (2) visual searching of litter and top-soil ants by hand sifting (3) visual searching of ant colonies, (4) visual searching of foraging workers. In total, 109 species in 44 genera from 9 subfamilies were collected. The number of species collected from litter and soil in Purwodadi was remarkably smaller than that in Bogor, while the ant species diversity on tree trunks was similar to that of Bogor. Of the109 species, 68 were common to Bogor. Arboreal ants were more similar than ground ants between Bogor and Purwodadi.

The Impact of Fallen Tree Trunks on the River Flow Path on the Characteristics of the Hyporheic Area Using Comsol Software (Case Study: Garmabdasht River, Gorgan)

The Impact of Fallen Tree Trunks on the River Flow Path on the Characteristics of the Hyporheic Area Using Comsol Software (Case Study: Garmabdasht River, Gorgan)

The The ISOLATION AND MOLECULAR IDENTIFICATION OF BACTERIA ASSOCIATED WITH SOIL SURROUNDING THE ROOT OF CITRUS (Citrus sinensis L.) TREE

Microbiological processes occur in the soil around the roots of land plants. This dynamic area, where plants and microorganisms interact, is crucial for the health and productivity of these plants. This study was aimed at isolating and molecularly identifying bacteria associated with the soil surrounding the root of an orange tree growing in the University of Ilorin, Nigeria to be able to know their roles, whether beneficial or harmful. Securing the soil is a way of having a food-secured world. Soil samples were collected at four different points from an orange tree trunk into separately labeled Ziploc bags using a separate sterile hand trowel for each collection. The labeled ziploc bags were brought to the University of Ilorin's Biology Laboratory for physicochemical analysis of the soil sample and isolation of bacteria using serial dilution method. Results of the physicochemical assessment of the bulked sample indicated that it is a sandy-loam soil with pH value of 6.7 (slightly neutral). Four bacterial species, i.e. Bacillus cereus, Lysinibacillusmacroides, Leucobacterkomogatae, and Alcaligenesfaecalis were isolated and identified molecularly. According to existing literature, the four bacterial species identified in this study are known for their abilities to enhance plant development and solubilize phosphorus, which are crucial for improving supply of nutrient.