Tree-ring Stable Carbon Research Articles

Central European Agroclimate over the Past 2000 Years

Abstract Central Europe has experienced a sequence of unprecedented summer droughts since 2015, which had considerable effects on the functioning and productivity of natural and agricultural systems. Placing these recent extremes in a long-term context of natural climate variability is, however, constrained by the limited length of observational records. Here, we use tree-ring stable oxygen and carbon isotopes to develop annually resolved reconstructions of growing season temperature and summer moisture variability for central Europe during the past 2000 years. Both records are independently interpolated across the southern Czech Republic and northeastern Austria to produce explicit estimates of the optimum agroclimatic zones, based on modern references of climatic forcing. Historical documentation of agricultural productivity and climate variability since 1090 CE provides strong quantitative verification of our new reconstructions. Our isotope records not only contain clear expressions of the medieval (920–1000 CE) and Renaissance (early sixteenth century) droughts, but also the relative influence of temperature and moisture on hydroclimatic conditions during the first millennium (including previously reported pluvials during the early third, fifth, and seventh centuries of the Common Era). We conclude that Czech agricultural production has experienced significant extremes over the past 2000 years, which includes periods for which there are no modern analogs. Significance Statement As temperatures increase, droughts are becoming a growing concern for European agriculture. Our study allows recent extremes to be contextualized and helps to better the understanding of potential drivers. Stable carbon and oxygen isotopes in oak tree rings were analyzed to reconstruct year-to-year and longer-term changes in both temperature and moisture over central Europe and the past 2000 years. We combine these proxy-based climate reconstructions to model how well crops were growing in the past. The early fifth and the early sixteenth centuries of the Common Era were most likely characterized by extreme conditions beyond what has been experienced in recent decades. Our reconstructions of natural variability might be used as a baseline in projections of future conditions.

The North American Monsoon buffers forests against the ongoing megadrought in the Southwestern United States.

The US Southwest has been entrenched in a two-decade-long megadrought (MD), the most severe since 800 CE, which threatens the long-term vitality and persistence of regional montane forests. Here, we report that in the face of record low winter precipitation and increasing atmospheric aridity, seasonal activity of the North American Monsoon (NAM) climate system brings sufficient precipitation during the height of the summer to alleviate extreme tree water stress. We studied seasonally resolved, tree-ring stable carbon isotope ratios across a 57-year time series (1960-2017) in 17 Ponderosa pine forests distributed across the NAM geographic domain. Our study focused on the isotope dynamics of latewood (LW), which is produced in association with NAM rains. During the MD, populations growing within the core region of the NAM operated at lower intrinsic and higher evaporative water-use efficiencies (WUEi and WUEE , respectively), compared to populations growing in the periphery of the NAM domain, indicating less physiological water stress in those populations with access to NAM moisture. The disparities in water-use efficiencies in periphery populations are due to a higher atmospheric vapor pressure deficit (VPD) and reduced access to summer soil moisture. The buffering advantage of the NAM, however, is weakening. We observed that since the MD, the relationship between WUEi and WUEE in forests within the core NAM domain is shifting toward a drought response similar to forests on the periphery of the NAM. After correcting for past increases in the atmospheric CO2 concentration, we were able to isolate the LW time-series responses to climate alone. This showed that the shift in the relation between WUEi and WUEE was driven by the extreme increases in MD-associated VPD, with little advantageous influence on stomatal conductance from increases in atmospheric CO2 concentration.

Differences in the Responses of Tree-Ring Stable Carbon Isotopes of L. sibirica and P. schrenkiana to Climate in the Eastern Tianshan Mountains

The eastern Tianshan Mountains are located in the arid interior of Asia, where tree growth is especially sensitive to climate. The ratio of stable carbon isotopes (δ13C) in the tree rings can provide information on changes in atmospheric CO2 concentrations, water availability, and physiological processes within the tree. In particular, the use of tree-ring δ13C values as a proxy for past atmospheric CO2 concentrations has gained widespread acceptance. In this study, detrended stable carbon isotope chronologies (13Ccorr) of Larix sibirica Ledeb. and Picea schrenkiana Fisch. et Mey was established using tree-ring samples from high elevations in the eastern Tianshan Mountains of Xinjiang, China. The relationships between the tree-ring 13Ccorr and different climatic factors were explored using the correlation function and collinearity analysis. Our results demonstrate that the tree-ring δ13Ccorr of L. sibirica is significantly and negatively correlated with precipitation and relative humidity during the growing season. The main climate factor affecting the stable carbon isotope fractionation of L. sibirica during the growing season is relative humidity during the growing season. The tree-ring δ13Ccorr of P. schrenkiana is significantly and negatively correlated with the mean temperature, mean minimum temperature, precipitation, and vapor pressure deficit from the end of the previous growing season and throughout the current growing season, especially in summer. However, it is significantly and positively correlated with relative humidity, indicating that the relationship between the climate factors and the tree-ring stable carbon isotope fractionation of P. schrenkiana is more complex. Further analysis showed that summer temperature and summer precipitation jointly controlled the tree-ring stable carbon isotope fractionation of P. schrenkiana at a high elevation. This research has important implications for our understanding of past and future climate change, as well as for the development of effective strategies to mitigate and adapt to these changes. This study also contributed to the development of a more in-depth understanding of the effects of climate change on tree growth in extremely arid environments and provided evidence to support effective forest management in arid regions.

The effects of intrinsic water-use efficiency and climate on wood anatomy.

Climate warming may induce growth decline in warm-temperate areas subjected to seasonal soil moisture deficit, whereas increasing atmospheric CO2 concentration (Ca) is expected to enhance tree growth. An accurate understanding of tree growth and physiological processes responding to climate warming and increasing Ca is critical. Here, we analyzed tree-ring stable carbon isotope and wood anatomical traits of Pinus tabuliformis from Qinling Mountains in China to understand how lumen diameter (LD) determining potential hydraulic conductivity and cell-wall thickness (CWT) determining carbon storage responded to climate and Ca. The effects of climate and Ca on intrinsic water-use efficiency (iWUE) were isolated, and iWUE values due to only-climate (iWUEClim) and only-CO2 effects (iWUECO2) were obtained. During a low-iWUE period, the influences of climate on earlywood (EW) LD and latewood (LW) CWT prevailed. During a high-iWUE period, CO2 fertilization promoted cell enlargement and carbon storage but this was counteracted by a negative influence of climate warming. The limiting direct effects of iWUEClim and indirect effects of climate on EW LD were greater than on LW CWT. P. tabuliformis in temperate forests will face a decline of growth and carbon fixation, but will produce embolism-resistant tracheids with narrow lumen responding to future hotter droughts.

Tree-Ring Stable Carbon Isotope-Based Mean Maximum Temperature Reconstruction in Northwest China and Its Connection with Atmospheric Circulations

The inter-annual stable carbon isotope ratio (δ13C) of three tree-ring cores of P. euphratica (Populus euphratica Oliv.) was determined from Ejina Oasis in Northwest China. A robust and representative δ13C chronology is generated from the three δ13C series using an arithmetic mean method. After eliminating the influence of the δ13C from elevated atmospheric carbon dioxide (CO2) concentration, we obtained a carbon isotopic discrimination (Δ13C) chronology. According to the significant correlation between the tree-ring Δ13C and instrumental data, we reconstructed the mean maximum temperature anomalies from previous December to current September (TDS) for the period 1901–2011. The reconstruction explained 43.6% of the variance over the calibration period. Three high-temperature periods (1929–1965, 1972–1974, and 1992–2006) and three low-temperature periods (1906–1926, 1966–1968, and 1975–1991) were found in the reconstructed series. Comparisons between the reconstructed TDS and the observed mean temperature from previous December to current September in Anxi meteorological station and the temperature index in north-central China demonstrated the reconstructed TDS has the advantage of reliability and stability. The significant spatial correlation declared that the reconstruction has a broad spatial representation and can represent the temperature variation characteristics in a wide geographical area. In addition, we found that the area of Ejina Oasis is smaller (larger) when the mean maximum temperature is higher (lower), which may be due to a conjunction effect of natural and anthropogenic activities. Significant periodicities and correlations suggested that the TDS variations in Ejina Oasis were regulated by solar radiation and atmospheric circulations at the interannual and interdecadal time scales.

Responses of Tree Growth and Intrinsic Water Use Efficiency to Environmental Factors in Central and Northern China in the Context of Global Warming

The Loess Plateau is a fragile ecological zone that is sensitive to climate change. The response, adaptation, and feedback of tree growth in forest ecosystems to global warming and CO2 enrichment are urgent scientific issues. Intrinsic water use efficiency (iWUE) is an important indicator for understanding forest ecosystem adaptability to climate change and CO2 enrichment. In this study, tree-ring width, tree-ring stable carbon isotope ratio (δ13C), and iWUE of P. tabulaeformis Carr. were established. Climate response analysis showed that temperature was the main limiting factor affecting radial tree growth and that relative humidity significantly affected the stable carbon isotope fractionation of tree rings. During 1645–2011, the iWUE increased by 27.1%. The responses of iWUE to climate factors and atmospheric CO2 concentrations (Ca) showed that the long-term variation in iWUE was affected by Ca, which could explain 69% of iWUE variation, and temperature was the main factor causing iWUE interannual variation. The ecosystem of P. tabulaeformis showed a positive response to rising Ca, as its carbon sequestration capacity increased. In response to global warming and CO2 enrichment, rising Ca promoted increases in iWUE but ultimately failed to offset the negative impact of warming on tree growth in the study area.

Investigation of age trends in tree-ring stable carbon and oxygen isotopes from northern Fennoscandia over the past millennium

Although tree-ring stable carbon (δ13C) and oxygen (δ18O) isotopes are increasingly used for climate reconstructions, it remains unclear whether isotopic ratios from the two chemical elements and different tree species exhibit age-related trends that require removal prior to any paleoclimatic interpretation. Here, we present 2,355 δ13C and 2,237 δ18O decadal measurements of living and relict Scots pines (Pinus sylvestris L.) from northern Fennoscandia to investigate the occurrence of isotope-specific age trends at both the individual tree and chronology level between 941 and 2010 CE, together with total-ring width and maximum density data. We show that δ13C values increase by ∼0.035‰ per 10 years of tree age, and therefore require detrending, which is not the case for δ18O that only contains minor changes related to age. This study provides independent evidence for the unique paleoclimatic value of stable δ18O isotopic ratios from the cellulose of living and relict pine wood to reconstruct high-to low-frequency climate variability. Conversely, caution is advised when information from diverse tree-ring parameters, species and regions is combined in multi-proxy climate reconstructions.

Tree-Ring Stable Carbon Isotope as a Proxy for Hydroclimate Variations in Semi-Arid Regions of North-Central China

Carbon and oxygen isotope ratios (δ13C and δ18O) were measured in annual tree-ring cellulose samples dated from 1756 to 2015 CE. These samples were extracted from Chinese pine (Pinus tabulaeformis Carr.) trees located in a semi-arid region of north-central China. We found that tree-ring δ13C and δ18O values both recorded similar climatic signals (e.g., temperature and moisture changes), but found that tree-ring δ13C exhibited a stronger relationship with mean temperature, precipitation, average relative humidity, self-calibrating Palmer drought severity index (scPDSI), and standard precipitation evaporation index (SPEI) than δ18O during the period 1951–2015 CE. The strongest correlation observed was between tree-ring δ13C and scPDSI (previous June to current May), which explains ~43% of the variance. The resulting 130-year reconstruction reveals severe drought events in the 1920s and a sustained drying trend since the 1980s. This hydroclimate record based on tree-ring δ13C data also reveals similar dry and wet events to other proxy data (i.e., tree-ring width and historical documentation) that have allowed reconstructions to be made across the northern fringe of the Asian summer monsoon region. Our results suggest that both large-scale modes of climate variability (e.g., El Niño-Southern Oscillation, Pacific Decadal Oscillation, and North Atlantic Oscillation) and external forcing (e.g., solar variability) may have modulated moisture variability in this region. Our results imply that the relationship between tree-ring δ18O and local climate is less well-characterized when compared to δ13C and may be affected more strongly by the influences of these different atmospheric circulation patterns. In this semi-arid region, tree-ring δ13C appears to represent a better tool with which to investigate historical moisture changes (scPDSI).

Response of Temperature-limited Forests to Recent Moisture Changes Derived from Tree-ring Stable Carbon Isotopes

Response of Temperature-limited Forests to Recent Moisture Changes Derived from Tree-ring Stable Carbon Isotopes

Recent European drought extremes beyond Common Era background variability

Europe’s recent summer droughts have had devastating ecological and economic consequences, but the severity and cause of these extremes remain unclear. Here we present 27,080 annually resolved and absolutely dated measurements of tree-ring stable carbon and oxygen (δ13C and δ18O) isotopes from 21 living and 126 relict oaks (Quercus spp.) used to reconstruct central European summer hydroclimate from 75 bce to 2018 ce. We find that the combined inverse δ13C and δ18O values correlate with the June–August Palmer Drought Severity Index from 1901–2018 at 0.73 (P < 0.001). Pluvials around 200, 720 and 1100 ce, and droughts around 40, 590, 950 and 1510 ce and in the twenty-first century, are superimposed on a multi-millennial drying trend. Our reconstruction demonstrates that the sequence of recent European summer droughts since 2015 ce is unprecedented in the past 2,110 years. This hydroclimatic anomaly is probably caused by anthropogenic warming and associated changes in the position of the summer jet stream. European summer droughts in recent years are anomalously severe compared with those of the previous 2,000 years, according to a synthesis of annually resolved tree-ring carbon and oxygen isotope records.

The dendroclimatic value of oak stable isotopes

Tree-ring stable oxygen and carbon isotope ratios (δ18O and δ13C) are an important archive for climate reconstructions. However, it remains unclear whether the polyvinyl acetate emulsion, often used for the preservation and fixation of wood samples, influences δ18O and δ13C signals. Further uncertainties are associated with the possible effects of geographical origin and cambial age of historical samples. Here, we present annually-resolved and absolutely-dated δ18O and δ13C measurements of 21 living oaks (Quercus robur and Q. petraea) from the Czech Republic. We find that the δ18O and δ13C signals in the extracted alpha-cellulose are not affected by polyvinyl acetate treatment. Covering the entire 20th century and reaching until 2018 CE, our dataset reveals spatial and temporal coherency within and between the individual δ18O and δ13C chronologies of different oak species, sample locations, and tree ages. Highly significant (p < 0.01) Pearson’s correlation coefficients of the site-specific δ13C and δ18O chronologies range from 0.48–0.77 and 0.36–0.56, respectively. The isotopic inter-series correlations of Q. robur and Q. petraea from the same site are 0.75 and 0.43 for the mean δ13C and δ18O values, respectively. Significant (p < 0.01) correlations of 0.49 and 0.84 are found for δ13C and δ18O, respectively, when all measurements from all sampling locations and tree ages are included. Our study shows that non-pooled oak δ18O and δ13C measurements from both species, different locations, and diverse tree ages can be combined into robust isotopic chronologies for climate reconstructions.

Retrospective Analysis of Tree Decline Based on Intrinsic Water-Use Efficiency in Semi-Arid Areas of North China

Long-term tree growth is significantly affected by climate change, which have become a global concern. Tree-ring width and isotopic information can show how trees respond to climate change on a long-term scale and reveal some phenomena of tree decline or death. In this study, we used isotopic techniques and investigated annual changes in carbon isotope composition and tree-ring width of Populus simonii Carr. in Zhangbei, as well as trends in tree-ring carbon discrimination (Δ13C) and iWUE in normal, mildly declining and severely declining trees, in order to make a retrospective analysis and further understand the process of tree decline. We found that there were significant differences (p &lt; 0.01 **) in δ13C, Δ13C, ci and iWUE at different decline stages, meaning that the δ13C and iWUE could be new indicators of tree health. The iWUE of all groups increased significantly, while the growth rate of declined P. simonii was much higher than that of normal growth P. simonii. According to the analysis, there may be a threshold of iWUE for healthy trees, which once the threshold value is exceeded, it indicates that trees are resistant to adversity and their growth is under stress. Similarly, the changing trend of BAI supports our conclusion with its changes showed that tree growth became slower and slower as degradation progressed. iWUE inferred from tree-ring stable carbon isotope composition is a strong modulator of adaptation capacity in response to environmental stressors under climate change. Elevated annual temperatures and increased groundwater depth are all contributing to the decline of P. simonii in north China.

Disentangling the effects of atmospheric CO2 and climate on intrinsic water-use efficiency in South Asian tropical moist forest trees.

Due to the increase in atmospheric CO2 concentrations, the ratio of carbon fixed by assimilation to water lost by transpiration through stomatal conductance (intrinsic water-use efficiency, iWUE) shows a long-term increasing trend globally. However, the drivers of short-term (inter-annual) variability in iWUE of tropical trees are poorly understood. We studied the inter-annual variability in iWUE of three South Asian tropical moist forest tree species (Chukrasia tabularis A.Juss., Toona ciliata M. Roem. and Lagerstroemia speciosa L.) derived from tree-ring stable carbon isotope ratio (δ13C) in response to variations of environmental conditions. We found a significantly decreasing trend in carbon discrimination (Δ13C) and an increasing trend in iWUE in all the three species, with a species-specific long-term trend in intercellular CO2 concentration (Ci). Growing season temperatures were the main driver of inter-annual variability of iWUE in C. tabularis and L. speciosa, whereas previous year temperatures determined the iWUE variability in T. ciliata. Vapor pressure deficit was linked with iWUE only in C. tabularis. Differences in shade tolerance, tree stature and canopy position might have caused this species-specific variation in iWUE response to climate. Linear mixed effect modeling successfully simulated iWUE variability, explaining 41-51% of the total variance varying with species. Commonality analysis revealed that temperatures had a dominant influence on the inter-annual iWUE variability (64-77%) over precipitation (7-22%) and atmospheric CO2 concentration (3-6%). However, the long-term variations in iWUE were explicitly determined by the atmospheric CO2 increase (83-94%). Our results suggest that the elevated CO2 and concomitant global warming might have detrimental effects on gas exchange and other physiological processes in South Asian tropical moist forest trees.

Growth decline of Pinus Massoniana in response to warming induced drought and increasing intrinsic water use efficiency in humid subtropical China

Pinus Massoniana is the most widely distributed coniferous species in southern China and one of the most distributed species for plantation in China. It is not uncertain about the responses of tree growth to the combined effects of regional drying and the increase in the intrinsic water-use efficiency (iWUE) due to increased atmospheric CO2. This study addressed this issue by comparing the tree growth patterns as represented by three tree-ring width chronologies with climate variables and three iWUE series derived from three tree-ring stable carbon isotope discrimination chronologies (Δ13C) from Pinus Massoniana in Daiyun Mountain, central Fujian province of China. Among these chronologies, we reported the first tree-ring carbon isotope discrimination chronologies (Δ13C) from Fuzhou area spanning last 210 years. It was found that tree radial growth is mainly limited by dry condition from May to October. Growth limitation by cold condition was only found in one high altitude site (780m) in early spring and late autumn. The tree-ring carbon discrimination was enhanced under conditions with low relative humidity and sufficient sunshine in late summer and autumn. In general, the iWUE showed a significantly increasing trend since the 1850s for all the sites in response to the increase in atmospheric CO2. However, the growth promotion of the increased iWUE on tree growth could not compensate the growth limitation caused by drought. Especially since the 1960s, a growth decline was found at two drought stressed sites at low altitudes. On the other hand, the increase in temperature of spring and autumn and iWUE has most likely enhanced tree growth at the high altitude site.

Instantaneous and long-term CO2 assimilation of Platycladus orientalis estimated from 13C discrimination

• A simple, nondestructive method to estimate CO 2 assimilation. • The method combined sap flow measurements and stable carbon isotope discrimination. • The method could estimate CO 2 assimilation on different time scales. Long-term forest productivity and carbon sequestration capacity are affected by climate change, and have become a global concern. In this study, we provide a simple and nondestructive method to determine tree CO 2 assimilation across multiple time scales. The new method combines sap flow and stable carbon isotope discrimination ( Δ 13 C) measurements to estimate carbon assimilation. We analyzed variability and conducted paired-sample t -tests to compare CO 2 assimilation estimated from gas exchange measurements and the new approach to verify its accuracy and applicability. Gas exchange and isotopic measurements both showed that the CO 2 assimilation rate in the morning was higher than in the afternoon, and peak values occurred around 10–11 a.m., which may have been due to nocturnal water storage and higher stomatal conductance in the morning. Diurnal, monthly, and annual CO 2 assimilation of P. orientalis variations were related to water supply conditions. Compared with previous research, we experimented using tree-ring stable carbon isotope discrimination ( Δ 13 C) and sap flow measurement to estimate annual CO 2 assimilation and the results were consistent with other traditional methods. Platycladus orientalis is effective and responsive to water supply, which explained why it adapts well in semi-arid areas. The new method for estimating CO 2 assimilation was accurate and applicable in semi-arid areas around Beijing.

Divergent growth responses to warming and drying climates between native and non-native tree species in Northeast China

Increased intrinsic water use efficiency enhances tree growth of native species, but not that of non-native species under warming and drying climates in Northeast China. Climate change significantly affects forest ecosystems. However, little is known about whether non-native and native tree species show similar responses to global warming. We found different trends in the basal area increment (BAI) and tree-ring stable carbon isotope ratio (δ13C) of two non-native (Pinus sylvestris var. mongolica and Populus × xiaozhuanica) and two native (Pinus tabuliformis and Ulmus pumila) tree species during the warming and drying periods from 1985 to 2014. The BAI of non-native tree species was stable, whereas that of the native tree species exhibited a significant increase. A significant increase in tree-ring δ13Ccorr (corrected for atmospheric changes in δ13C) for non-native tree species indicated increasing water stress. The intrinsic water use efficiency (iWUE, derived from tree-ring δ13C) of both non-native and native tree species increased significantly. However, the magnitude of the increase in iWUE was higher in non-native tree species than in native tree species, indicating that non-native tree species suffered stronger water stress. Increasing iWUE but no increase in BAI for non-native tree species suggested that water stress reduced stomatal conductance and, consequently, reduced carbon uptake. In contrast, increased iWUE accompanied by an enhanced BAI for native tree species indicated an increase in photosynthetic capacity induced by CO2 fertilization. These findings suggest that non-native tree species would experience greater mortality under extreme drought conditions once water stress passes a physiological threshold. However, native tree species would suffer only slightly due to benefiting from CO2 fertilization.

Tree-ring isotopes capture interannual vegetation productivity dynamics at the biome scale

Historical and future trends in net primary productivity (NPP) and its sensitivity to global change are largely unknown because of the lack of long-term, high-resolution data. Here we test whether annually resolved tree-ring stable carbon (δ13C) and oxygen (δ18O) isotopes can be used as proxies for reconstructing past NPP. Stable isotope chronologies from four sites within three distinct hydroclimatic environments in the eastern United States (US) were compared in time and space against satellite-derived NPP products, including the long-term Global Inventory Modeling and Mapping Studies (GIMMS3g) NPP (1982–2011), the newest high-resolution Landsat NPP (1986–2015), and the Moderate Resolution Imaging Spectroradiometer (MODIS, 2001–2015) NPP. We show that tree-ring isotopes, in particular δ18O, correlate strongly with satellite NPP estimates at both local and large geographical scales in the eastern US. These findings represent an important breakthrough for estimating interannual variability and long-term changes in terrestrial productivity at the biome scale.

Tree-ring δ18O from an Alpine catchment reveals changes in glacier stream water inputs between 1980 and 2010

ABSTRACTThe tree-ring stable carbon and oxygen isotope chronologies from two forest sites located in the Forni Glacier forefield (Italy)—one along the glacier stream (GL) and the other toward the valley slope (SL)—were analyzed with the aim of disentangling the precipitation and glacier meltwater inputs in source water δ18O, as reflected by the tree-ring cellulose δ18O. The cellulose δ18O from the GL trees has a negative correlation with winter and summer temperatures, whereas the cellulose δ18O from the SL trees has a positive correlation with precipitation δ18O. The isotopic signature of the source water at the GL site is also influenced by waters of glacial origin, as confirmed by the 18O-depleted glacier meltwater inputs (GMWI_δ18O) estimated by means of an isotope model. The GMWI_δ18O values are consistent with the mean difference measured between the δ18O in the glacier stream and in the precipitation and the winter and summer temperature explains up to 37 percent of the GMWI_δ18O variance. Our results show an increasing influence of glacier meltwater throughout the past decade for the GL site. Our analysis opens new opportunities to reconstruct changes in water regimes of the glacier streams by means of the tree-ring cellulose δ18O.

Tree-ring stable carbon isotope-based June–September maximum temperature reconstruction since AD 1788, north-west Thailand

The first study of tree-ring stable carbon isotopes in Thailand has demonstrated that stable carbon isotope in northwestern Thailand represents a promising proxy for the temperature reconstruction of core-monsoon periods. A tree-ring δ13C chronology was constructed based on four cores covering the period of 1788–2013. After removing the long-term decreasing trend reflecting atmospheric CO2 concentrations, the ∆13C chronology was able to capture both temperature and hydro-climate signals. ∆13C chronology showed particularly strong and significant negative correlation (r = –0.62, p < 0.0001) with June–September maximum temperature (CRU TS 3.24). The maximum temperature was reconstructed, which explained 37.8% of the variance in the instrumental maximum temperatures over the period of 1901–2013. The maximum temperature reconstruction revealed that four cooler and three warmer periods, as well as a slightly increasing temperature trend, occurred during the late seventeenth to mid-eighteenth centuries, which were followed by severe temperature fluctuations during the twentieth century century. While the sea surface temperature anomaly in the Indian Ocean might not affect the maximum temperature, its unstable relationship with the El Niño-Southern Oscillation (ENSO) was detected. In addition, a close relationship was observed between the maximum temperature and ENSO during the negative phase of the Pacific Decadal Oscillation (PDO), but this relationship was lost during the positive phase of the PDO.

Long-term trends in leaf level gas exchange mirror tree-ring derived intrinsic water-use efficiency of Pinus cembra at treeline during the last century

The ability of treeline conifers in the Central European Alps to cope with recent climate warming and increasing CO2 concentration is still poorly understood. We determined basal area increment (BAI) and tree ring stable carbon isotope ratios (δ13C) of Pinus cembra trees from 1925 through 2013. Stable isotope ratios and BAI were compared with leaf level gas exchange measurements carried out in situ between 1934 and 2012, and thus, provided new insights into long-term trends of tree-ring derived intrinsic water-use efficiency (iWUE). Mature P. cembra trees at treeline responded to increasing Ca and air temperature with a parallel increase in maximum net CO2 uptake rate at ambient CO2 (Amax) and tree-ring-derived intercellular CO2 partial pressure (Ci). Amax tripled and was positively correlated to BAI and Ci. The latter increased in parallel with ambient CO2 concentration and stomatal conductance. In contrast to the instantaneous gas exchange parameters, δ13C derived iWUE informs about the long-term changes in the carbon water relations. These data showed three changes in the iWUE chronosequences, which could be identified with different long term gas exchange patterns: (1) from stomatal controlled functioning from 1925 to 1981, to a situation where (2) both net CO2 fixation (A) and leaf conductance for water vapour (gw), responded to the environment from 1982 to 1997, and (3) back to a stomata controlled pattern over iWUE from 1998 onwards. This temporal pattern was also mirrored in leaf level gas exchange assessments, suggesting a parallel increase of A and gw of P. cembra at treeline during the last nine decades.