Treatment T1 Research Articles

Effect of different levels of nitrogen in combination with nano urea on growth, yield and quality of crossandra (Crossandra infundibuliformis L.) cv. Lakshmi

An investigation on the effect of different levels of nitrogen in combination with nano urea on growth, yield and quality of crossandra (Crossandra infundibuliformis L.) cv. Lakshmi was carried out in the Floriculture unit, Department of Horticulture, Faculty of Agriculture, Annamalai University, Chidambaram during the year 2022-2024. The experiment was laid out in Randomized Block Design with 14 treatments and 3 replications. Among the different treatments, the treatment T5 (100% PK + 75% N through urea + 0.4% Nano urea (3 sprays)) resulted in increased growth attributes such as plant height (85.21 cm), stem girth (7.95 cm), number of branches/plant (24.51), number of leaves/plant (99.29), leaf area (95.41 cm2) and chlorophyll content (0.840 mg/g). With regards to yield attributes, the treatment T8 (100% PK + 50% N through urea + 0.4% Nano urea (3 sprays)) showed early spike emergence (37.54 days), time taken for 1st flowering (44.83 days) with an increase in number of spikes/plant (36.27), spike length (14.21 cm) and number of flowers/spike (39.21). 100 flower weight (8.62 g), flower yield/plant (51.03 g), flower yield/plot (974.76 g/plot), estimated flower yield/ hectare (32.49 q/ha) and quality attributes like xanthophyll content (69.42 mg/g), carotenoid content (57.76 mg/g), shelf life of flowers (74.88 hours), longevity of flower in the plant (95.52 hours) and flowers with an excellent quality of visual scoring (9.16) were also observed the maximum in the treatment T8. The treatment control (T1) had the lowest value for all the growth, yield and quality attributes. Hence, it is concluded that the treatment T8 (100% PK + 50% N through urea + 0.4% Nano urea (3 sprays)) was found to be the best combination for improving the growth, yield and quality of crossandra cv. Lakshmi.

Influence of N, P, K, Rhizobium and Vermicompost on Soil Health Parameters and Yield of Green Gram (Vigna radiata L.) var. Samrat

An experiment was conducted on “Influence of N, P, K, Rhizobium and Vermicompost on Soil Health Parameters and Yield of Green Gram (Vigna radiata L.) var. Samrat”. during zaid season 2023-2024 at the central research farm, department research farm of Soil Science and Agricultural Chemistry, The design applied was 3x3 RBD having three levels each of vermicompost and N.P.K i.e., 0, 50, and 100% respectively and three levels of rhizobium treated seed @ 0, 50, 100%. The result obtained in treatment T9 (N25P50K25 kg ha-1 + vermicompost @ 5 t ha-1 and rhizobium @ 200g 10 kg-1) has showed a slight change in soil pH i.e., 6.79 and EC i.e., 0.208 dS m-1. The post-harvest soil samples have showed significant increase in pore space, water holding capacity and organic carbon i.e., about 49.20 %, 47.59 % and 0.49 % respectively. Whereas available N, P and K found to be 334.12 kg ha-1, 34.36 kg ha-1, and 204.28 kg ha-1 respectively. The increase in NPK (kg ha-1) was found to be significant among all treatments in green gram cultivation and soil quality improvement. The best results among all treatment combination with respect to plant height (65.76 cm), number of leaves plant-1 (44.34), number of branches plant-1 (14.58), number of grain pod-1 (14.28), number of pod plant-1 (42.11) and seed yield q ha-1 (16.67) were found in treatment combination T9 (N25P50K25 kg ha-1 + vermicompost @ 5 t ha-1 and Rhizobium @ 200g 10 kg-1. T9 gave the highest gross return with ₹ 1,49,030 and the benefit of ₹ 29,849.13 with highest cost benefit ratio i.e., 1:1.38. vermicompost along with other combinations has showed improvement in soil health as well as in crop development and yield. Study has also revealed that the application of N, P, and K with vermicompost was an excellent source for fertilization than sole fertilizers.

Enhancing Yield and Nitrogen Utilization of Boro Rice with Urea Super Granules

A field experiment was conducted at the Soil Science Field Laboratory of Bangladesh Agricultural University, Mymensingh during boro season to assess the comparative performance of prilled urea (PU) and urea super granule (USG) on the growth, yield, and nitrogen uptake of BRRI dhan29. The experiment was laid out in a randomized complete block design with three replications. The treatment combinations were T1: Control, T2: PU at 7 days after transplanting (DAT)+27 DAT+47 DAT, T3: PU at 10 DAT+30 DAT+50 DAT, T4: PU at 15 DAT+35 DAT+55 DAT, T5: USG at transplanting, T6: USG at 7 DAT, T7: USG at 10 DAT and T8: USG at 10 DAT+30 DAT where PU and USG were applied @ 216 kg N ha-1. PU was applied as surface broadcasting whereas USG was placed at 6-8 cm depth by hand between four hills at every alternate row. The results revealed that all the treatments showed better performances than control (T1). Results found that yield components, yield, and nitrogen uptake of BRRI dhan29 responded significantly to deep placement of USG than PU. Treatment T6 (USG at 7 DAT) produced the highest grain and straw yields of 6.56 t ha-1 and 6.95 t ha-1 respectively. Treatment T6 (USG at 7 DAT) also showed the highest grain yield increase (57.16%) and straw yield increase (52.37%) over control. The highest grain, straw, and total N uptake of 87.28, 32.71 and 120 kg ha-1 respectively were obtained in the treatment T6 (USG at 7 DAT). The overall results clearly indicate that deep placement of USG at 7 DAT in rice field increases rice yield and efficient uptake of the applied N. So, treatment T6 (USG at 7 DAT) could be recommended as best treatment for reducing N losses, conserving environment and improving sustainable production of rice.

Impact of Integrated Nutrient Management on Yield, Soil, and Plant Nutrient Status in Sweet Orange (Citrus sinensis L.) cv. Phule Mosambi

ABSTRACT Impact of integrated nutrient management on plant and soil nutrient status in sweet orange (Citrus sinensis L.) cv. Phule Mosambi was investigated for two consecutive years i.e. 2019 and 2020 at the Horticultural Research Farm, College of Horticulture, Anand Agricultural University, Anand. The experiment was laid out in completely randomized design (CRD) comprised of 11 treatments replicated thrice. Significantly higher yield was recorded with T11 [50% Recommended Dose of Nitrogen (RDN) + 20 kg farmyard manure (FYM)/tree +25% nitrogen (N) from Castor Cake + 10 ml Bio NPK Consortium + Micronutrient foliar spray (1% Grade IV)] (17.35 and 17.01 t/ha) which was at par with T10 (15.92 and 15.56 a t/ha) during 2019 and 2020, respectively. Amongst the treatments, T11 had increased the nutrient contents of soil as well as in plant and recorded maximum available nitrogen (N), available potassium (K), soil boron (B) content, plant nitrogen (N) content, plant iron (Fe) content, plant copper (Cu) content and plant boron (B) content in both the years whereas available phosphorous (P) was found higher in treatment T7 in both the years. Treatment T8 recorded highest soil copper (Cu) content in both the years. As far as plant zinc (Zn) content is concerned, it was found higher in treatment T9 in both the years.

Impact of Foliar Application of Boron on Yield and Yield Attributes, Assimilate Supply, Production Efficiency and Fertilizers Productivity of Indian Mustard (Brassica juncea) in Field Experiments

Field experiments were conducted for three consecutive rabi seasons from 2018 to 2020 to assess the impact of foliar application of boron fertilizer on yield and yield attributes, assimilate supply, production efficiency and fertilizers productivity of Indian mustard [Brassica juncea (L.) Czern. &Coss.]. The results showed that the number of primary branches/ plant (7.00 to 8.08), number of siliquae/plant (427.2 to 468.8), number of seeds/siliqua (13.03 to 14.01), test weight of seed (3.60 to 3.80 g) and plant dry matter production (38.10 to 41.20 g/plant) were significantly increased with T2 treatment (100% NPKS + foliar application of Boron @ 0.5% Borax) as compared to farmer’s practice T1 (100% NPKS + without boron) during three years of experiments. Significantly higher seed yield (22.5 to 24.7 q/ha) of the mustard crop was also reported with T2 (100% NPKS + foliar application of Boron @ 0.5% Borax) as compared to farmers’ practice T1 (100% NPKS + without boron) (seed yield 19 to 22.5 q/ha) during the studies. Similarly, Treatment T2 resulted in significantly higher production efficiency and assimilates supply of mustard crop than that of T1 (farmer’s practice). The fertilizer productivity in the mustard crop that is directly related to seed yield and the partial factor of productivity of N, P, K and S fertilizers (PFPNPKS) were also increased significantly with the T2 compared to farmers practiceT1(farmer’s practice), during 3 years of studies. The highest gross return and net return and benefit-cost ratio were obtained with T2 (100% NPKS + foliar application of Boron @ 0.5% Borax) treatment over T1 (100% NPKS + without boron).

Impact of Nutrient Management Practices on Exchangeable K, Ca, Mg, CEC and Correlations with Soil Properties Under Maize-wheat Cropping System

In order to study the impact of nutrient management practices on exchangeable K, Ca, Mg, CEC and its correlations with soil properties in two depths (0-15 cm and 16-30 cm) under maize-wheat cropping system, a long term experiment was conducted at farm research centre of department of soil science falling under the jurisdiction of Birsa Agricultural University. The experiment consisted of nine treatments replicated thrice in a randomized block design were selected for the study. The selected treatments were T1-control, T2-100% N only where 100% N was applied through Urea fertilizers,T3- FYM where 100% N was supplied through FYM (full dose 22 t/ha), T4-100% NP here 110 and 90 kg ha-1were supplied through Urea and SSP fertilizers, respectively, T5-100% NPK here 110:90:70 kg ha-1 were supplied through Urea: SSP: MOP fertilizers, T6- ½ (N+FYM)+P (A-X/2)+K (B-Y/2) (INM) where 50% of the nutrient need was supplied through Urea, SSP & MOP fertilizer and the rest 50% need through FYM and T7-100% NPK + Lime where NPK applied through Urea, SSP & MOP fertilizer along with lime application in LR once in 4 years, T8-Lime+ FYM+ P (A-X)+K (B-Y), the 100% N need of the crop was met by application of FYM whereas the P & K need was met by the application of SSP & MOP fertilizer, T9-Lime+N the 100% N dose was supplied through Urea along with lime. The results indicated that exchangeable K was found to be significantly highest in T8 than rest of the treatments followed by T7 and T5 while lowest was in T4 treatment in both depths respectively. The exchangeable Ca2+ was found to be highest in treatment T7 followed by T8 and T3 than rest of the other treated plot while lowest amount was found in T2 in both the depths. The trend of exchangeable magnesium was found to be significantly different in 0-15 cm and 15-30 cm soil depths as the highest amount was observed in treatments such as T8 followed by T3 and T6 in surface depths (0-15 cm) while in sub-surface depths (15-30 cm) the highest amount was found in treatments T3 followed by T8 and T6 while the lowest amount was found in T2 treated plot in both depths. Cation exchangeable capacity (CEC) is one the vital soil parameters concerning to soil fertility measured in terms of c mol (p+) kg-1 of soil and it is significantly varied in surface soils as well as in sub-surface soil depths. The CEC was significantly higher in FYM treated plot as compared to Non-FYM treated plot. Therefore, the highest CEC was recorded in T3 treated plot than rest of the other treatment while the lowest one was recorded in T2 plot in surface soils as well as sub-surface soils. The correlation coefficient (r) values worked out among the various soil parameters at these depth of soil where pH was significantly and positively correlated with exchangeable Ca2+ and exchangeable Mg2+. Similarly organic carbon shows positive correlation with exchangeable Mg2+ and exchangeable K+. Whereas EC and CEC shows non-significant but positive correlation with the parameters taken under study. The result of the present study will be highly useful for improving the soil fertility by using suitable and judicious combination of nutrient management with various cropping system side by side and hence provides a scientific scope for further research work.

Effect of Different INM Packages on Vegetative, Flowering, Yield and Economics of China Aster cv Kamini under High Hill Condition of Uttarakhand

The present investigation was conducted to develop a sustainable INM package for economical production China aster crop under high hill condition of Uttarakhand. The experiment was conducted at Floriculture farm, COH, VCSG, UUHF, Bhrasar from March to September 2022. The treatments were 12, replicate thrice in Randomized Complete Block Design. The results showed that maximum plant spread (22.86 cm), number of leaves plant–1 (219.33), leaf area (38.56 cm2), earliness in days taken to 1st bud appearance and flower opening (68.630 and 82.707days, respectively) and maximum flowering duration (23.430 days) were recorded in T7 (50% RDF + 25% FYM + 25% Vermicompost + Azotobacter (5 mL L–1)). However, maximum number of flowers plant-1 and plot–1 (36.80 and 441.53, respectively), stalk length (38.40 cm), flower diameter (7.26 cm), individual flower weight (3.86 g), flower yield plant–1 and bed–1 (142.89 g and 1.71 kg), seed yield plant–1 (3.330 g) and test weight (2.477 g), shelf and vase life (4.50 and 12.300 days, respectively) and C:B from flowers (1: 2.19) were recorded from the plants grown in plots applied with T8 (50% RDF + 25% FYM + 25% Neem cake + Azotobacter (5 mL L–1)). So, from the present investigation it can be concluded that treatment T8 was found effective in economical production of China aster cv Kamini under high hill condition of Uttarakhand.

Influence of Organic Inputs on the Performance of Strawberry (Fragaria x ananassa Duch.) cv Festival

An experiment was conducted to find out the effect of organic inputs on yield attributing characters and quality of strawberry cv. Festival was carried out in Garo Hills region of Meghalaya, India during 2022-2023 at Department of Horticulture, North-Eastern Hill University, Tura Campus in West Garo Hills District of Meghalaya. The experiment was laid out in Completely Randomized Design with eight different treatments which were replicated thrice. Treatment T2 (vermicompost 250g/plant) was found to be superior over other treatments for parameters like number of shoots (6.67-11.00), number of leaves (9.33-26.00), leaf area (31.50 cm2-104.50 cm2), total numbers of runners (7.00) and yield parameters like number of fruit set (13.00), fruit set percentage (81.61%), fresh fruit weight (16.55g), fruit length × diameter (34.00 mm × 30.00 mm) and yield of the plant (495.26g). Whereas, T1(vermicompost 300g/plant) recorded the highest in number of flowers (2.66-4.00), duration of flowering (107.00 days) and minimum days taken for flowering (43.00 days). Meanwhile, it was also recorded that T2(vermicompost 250g/plant) was found to have maximum total soluble solids (TSS) (10.47°Brix), total sugar (4.38%), reducing sugar (2.33%), non-reducing sugar (2.05%), titratable acidity (1.80%), ascorbic acid (16.46 mg/100g) and anthocyanin content (23.92 mg/100g). The overall findings indicate the potential of vermicompost (250g/plant) in organic production of strawberry.

Harnessing the power of native biocontrol agents against wilt disease of Pigeonpea incited by Fusarium udum

Fusarium wilt, caused by (Fusarium udum Butler), is a significant threat to pigeonpea crops worldwide, leading to substantial yield losses. Traditional approaches like fungicides and resistant cultivars are not practical due to the persistent and evolving nature of the pathogen. Therefore, native biocontrol agents are considered to be more sustainable solution, as they adapt well to local soil and climatic conditions. In this study, five isolates of F. udum infecting pigeonpea were isolated from various cultivars and characterized morphologically and molecularly. The isolate from the ICP 8858 cultivar displayed the highest virulence of 90%. Besides, 100 endophytic bacteria, 100 rhizosphere bacteria and three Trichoderma spp. were isolated and tested against F. udum isolated from ICP 8858 under in vitro conditions. Out of the 200 bacteria tested, nine showed highest inhibition, including Rb-4 (Bacillus sp.), Rb-11 (B. subtilis), Rb-14 (B. megaterium), Rb-18 (B. subtilis), Rb-19 (B. velezensis), Eb-8 (Bacillus sp.), Eb-11 (B. subtilis), Eb-13 (P. aeruginosa), and Eb-21 (P. aeruginosa). Similarly, Trichoderma spp. were identified as T. harzianum, T. asperellum and Trichoderma sp. Notably, Rb-18 (B. subtilis) and Eb-21 (P. aeruginosa) exhibited promising characteristics such as the production of hydrogen cyanide (HCN), cellulase, siderophores, ammonia and nutrient solubilization. Furthermore, treating pigeonpea seedlings with these beneficial microorganisms led to increased levels of key enzymes (POD, PPO, and PAL) associated with resistance to Fusarium wilt, compared to untreated controls. In field trials conducted for four seasons, the application of these potential biocontrol agents as seed treatments on the susceptible ICP2376 cultivar led to the lowest disease incidence. Specifically, treatments T2 (33.33) (P. aeruginosa) and T3 (35.41) (T. harzianium) exhibited the lowest disease incidence, followed by T6 (36.5) (Carbendizim), T1 (36.66) (B. subtilis), T4 (52.91) (T. asperellum) and T5 (53.33) (Trichoderma sp.). Results of this study revealed that, P. aeruginosa (Eb-21), B. subtilis (Rb-18) and T. harzianum can be used for plant growth promotion and management of Fusarium wilt of pigeonpea.

HOLOTHURIA SCABRA METHANOL EXTRACT INHIBITS CANCER GROWTH THROUGH TGF-β/PI3K/PTEN SIGNALING PATHWAY IN BREAST CANCER MICE MODEL.

Molecules and cytokines can be targeted in cancer therapy. Transforming growth factor-beta (TGF-β) is a cytokine that acts on protein kinase receptors in the plasma membrane. The signaling pathway of TGF-β can trigger the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) pathway, a signal transduction pathway important in cancer growth and development. However, this PI3K/AKT cascade can be inhibited by phosphatase and tensin homolog (PTEN) tumor suppressor genes. To determine the inhibitory effect of Holothuria scabra methanol extract (HSE) on breast cancer growth through the TGF-β/PI3K pathways and PTEN tumor suppressor gene on a breast cancer (BC) mice model. Female C57BL6 mice were subcutaneously injected with carcinogen DMBA 1 mg/kg body weight (BW) and fed a high-fat diet (HFD). Mice were randomly divided into five groups (n = 6): negative control (NC) administered with a standard diet, positive control (PC) administered with DMBA and HFD, and three treatment groups (T1, T2, and T3) treated with HSE doses of 0.33, 0.66, and 0.99 g/kg BW for 12 weeks. TGF-β concentration in the blood serum of mice was assessed by ELISA and the PIK3CA and PTEN gene expression by qRT-PCR. The treatment with HSE resulted in a significant decrease in TGF-β concentrations in the blood sera of treatment groups T1 (35.31 ± 17.33), T2 (43.31 ± 17.42), and T3 (48.67 ± 20.94) pg/mL compared to the PC group (162.09 ± 11.60) pg/mL (p < 0.001). However, only HSE at a dose of 0.99 g/kg BW decreased the PIK3CA gene expression (p = 0.026), and at a dose of 0.66 g/kg BW increased the PTEN expression up to 4.93-fold. HSE is capable of inhibiting the TGF-β/PIK3CA pathway and increasing the PTEN gene expression.

Effect of different organic sources and their combination on growth, yield and economics of millet crop- pearl millet

The present investigation was carried out to identify the effects of different combinations of organic nutrition and its role in the growth, yield and economics of the pearl millet variety Shri Ram Pro 90. From the above experimental finding, it may be concluded that the treatment T3 (100% Neem cake) was found to be best in terms of growth parameters like the highest number of effective tillers (6.82 tillers), tallest plant (128.48 cm) at 60 DAS and yield parameters like longest length of ear head (38.23 cm); thickest girth of ear head (15.57 cm); maximum number of grains per ear (2013.00 grains); highest grain yield per hectare (2787.79 kg/ha) of Pearl millet. The highest net return was found in the T2 (100% Vermicompost), followed by T3 (100% Neemcake).

Effect of Organic Manure and Biofertilizers on the Growth, Yield and Productivity of Guava under Meadow Orcharding

The study was conducted to investigate the effect of organic manure and biofertilizers on the growth, yield and productivity of guava under meadow orcharding at Horticulture Research Farm, Department of Agronomy, Naini Agricultural Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj. In this experiment, various organic sources (Farm yard manure (30 kg/plant/y), vermicompost (30 kg/plant/y) and neem cake (3 kg/plant/y) along with various biofertilizer combinations (Azotobacter, Azospirillum, PSB) were tested on five-year-old guava cultivar 'Allahabad Safeda' to study its effect on growth, fruiting and yield. The results obtained showed higher fruit weight (218.00 g), fruit length (7.60 cm), fruit diameter (7.42 cm), maximum number of fruits produced per plant (26.64 fruits/plant), maximum yield per plant of 5.81 kg/plant and maximum yield per ha (23.12 t/ha) with the application of T9. The highest total soluble solids (12°Brix) and total sugar content of fruit (8.26%) with the application of T9. Application of nutrients through organic along with biofertilizers improve soil health in terms of mean microbial population in the rhizosphere of root zone soil as compared to control. From the present study, the application of biofertilizers with the combination of organic manures has a beneficial effect on the availability of major nutrients in the soil to help in maintaining of the soil fertility and plant growth of the fruits at a desired level. It can be concluded that, for better growth, yield and quality guava fruits, the guava trees should be fertilized with (FYM (30kg/plant) +Azospirillum @250g/tree +PSB @250g/tree +Neem cake @3kg/tree) per year with treatment T9.

Economics of Fieldpea (Pisum sativum L.) as Influenced by Phosphorus Levels and Bio-fertilizers in Central Plains of Uttar Pradesh, India

A field experiment was conducted at the student instructional farm of CSAUA&amp;T, Kanpur, during the Rabi season of 2021-22 and 2022-23. Sandy loam was the texture of the experimental soil. There are twelve treatment involving combinations of phosphorus and biofertilizers with three replication of factorial randomized block design (FRBD). A fieldpea variety IPFD 6-3 was planted according to recommended agronomic practices. The results of these studies indicate that fieldpea cultivation over a two-year period is consistent with T12 (P90 R1 PSB1), the most economically effective treatment and also recorded the highest economics parametric values i.e., maximum cost of cultivation of ₹35850 &amp; ₹36700, gross return of ₹ 106055 &amp; ₹118535, net return of ₹70205 &amp; ₹ 81835 and benefit to cost ratios were 1.96 &amp; 2.23 followed by the treatment T11.

Bioprocess Design and Evaluation of Hydrothermal Hydrolysates from Sargassum sp. for Enhancing Arthrospira platensis Growth and Protein Content

The proliferation of Sargassum biomass in various coastal areas has led to environmental and socio-economic problems. However, due to their unique composition, these biomasses offer versatile applications, prompting research into their potential in third-generation biorefineries. In this study, the hydrothermal processing of Sargassum sp. was evaluated under specific conditions at 190 °C/50 min and 150 °C/30 min. The resulting hydrolysates (liquid phase) were used as alternative culture media for cultivation. Nine treatments for the cultivation of Arthrospira platensis were assessed, varying the concentration of hydrothermal hydrolysates (HH) at 190 °C/50 min: T1 (5% v/v), T2 (10% v/v), and T3 (15% v/v). T4 (5% v/v), T5 (10% v/v), and T6 (15% v/v), maintaining the same HH conditions, and with the addition of 0.7 g/L NaNO3; and treatments T7, T8, and T9 had concentrations of 5%, 10%, and 15% of HH, respectively, at 150 °C/30 min with the addition of 0.7 g/L NaNO3, respectively. Each treatment was inoculated with 15% (v/v) of A. platensis. Growth kinetics were performed by sampling every three days for 24 days. Quantification of soluble proteins was performed for the best conditions of biomass production. The microalgae demonstrated the ability to grow under mixotrophic medium conditions and to utilize the available carbon sources in the culture medium. Treatment 4 has the highest biomass, with an Xmax (g/L) of 1.94 ± 0.06 and a protein production of 24.17 ± 0.86% (w/w). Therefore, this microalgal biomass can be used in the food matrix according to the biorefinery concept.

Effects of Different Qualities of Light and Phytohormones on Seed Germination, Seedling Growth and Establishment of Papaya (Carica papaya) cv. Pusa Nanha

The present study was undertaken to study the effects of different qualities of light and phytohormones (GA3 @ 200ppm) on papaya seed germination, seedling growth, and establishment. The experiment was laid in Randomized Block Design. The experiment comprises of eight treatments viz. T0 {Natural Light (95000 lux)}, T1{Shade Net/Green House (Light 21500 lux)}, T2 {Polyhouse (light 7750 lux)}, T3 {Partially shaded area (light 2800 lux)}, T4 {Fully shaded area (light 880 lux)}, T5 {Incandescent Light (290 lux)}, T6 {Fluorescent Light (3000 lux)}, T7 {Growth Chamber Light (light 2500 lux)}. Among different treatments T6 performed best in terms of Seed germination, Seedling growth and Establishment of Papaya. The findings revealed that treatment T6 {Fluorescent Light (3000 lux)} + GA3 (200 ppm) performed best in term of Days to Germination (8 Days), Germination percentage (94.44%), Number of leaves (9.6), Plant height (16.23 cm), stem vigour index (1444.93), Leaf area (394.4 cm2), Chlorophyll content (41.47 SPAD value) and stem girth (0.37 cm), Establishment Percentage (93.33%). The highest B:C ratio was found in the same Treatment, T6 {Fluorescent Light (3000 lux)} + GA3 (200 ppm), with 3.5.

BIOESTIMULANTE Y TIEMPOS DE IMBIBICIÓN SOBRE LA GERMINACIÓN DE SEMILLAS DE MARACUYÁ (Passiflora edulis S., Passifloraceae

&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Passion fruit (&lt;em&gt;Passiflora edulis&lt;/em&gt; S.) is a tropical fruit of socio-economic interest in Peru, used as an ingredient in the food industry, esthetics and medicine. However, this crop has a low germination power, so it is necessary to carry out pre-germination treatments to increase the germination percentage and the initial development of the seedlings. &lt;strong&gt;Objective:&lt;/strong&gt; to evaluate the effect of different biostimulants at 12 h and 24 h of hydration on passion fruit seed germination. &lt;strong&gt;Methodology: &lt;/strong&gt;passion fruit seeds of large and round fruits, free of pathogens, were collected. The aryl-free seeds were treated: T0 (distilled water), T1 (sulfur 25 g/10 L), T2 (sulfur 50 g/10 L), T3 (biol 3%), T4 (biol 5%), T5 (cinnamon 25%), T6 (cinnamon 50%), T7 (buttermilk 25%) and T8 (buttermilk 50%) for 12 h and 24 h imbibition. The variables evaluated were: water absorbed, imbibition rate (IR), germination percentage (GP), germination speed (GS), germination index (GI), first day of germination (FDG). &lt;strong&gt;Results:&lt;/strong&gt; The highest water absorption occurred in the first 9 hours. Treatments T4, T5 and T6 significantly improved the GP, GS, GI, FDG at 12 h imbibition. However, at 24 h imbibition these values decreased. GP presented a high positive correlation with GS and GI. Likewise, GS presented a high negative correlation with FDG. &lt;strong&gt;Implications: &lt;/strong&gt;The use of biostimulants increases germination. &lt;strong&gt;Conclusions:&lt;/strong&gt; Cinnamon extract at 25% and 50%, and biol 5% at 12 h of imbibition have significantly improved the variables evaluated compared to imbibition at 24 h. The positive correlation between the variables indicates a direct relationship between them.&lt;/p&gt;

Effect of INM on Growth Indices, Yield Attributes, Yield and Economics of Wheat (Triticum aestivum L. emend. Fiori &amp; Paol.)

The experiment was conducted at farmers field in Haridwar district of Uttarakhand during 2019-20 and 2020-21. The experiment consisted of eleven treatments viz. control (T1), 100 % RDF (T2), FYM @ 10 t/ha (T3), VC @ 5t/ha (T4), 75 % RDF + FYM @ 10t/ha (T5), 75 % RDF + VC @ 5 t/ha (T6), 50 % RDF + FYM @ 10 t/ha (T7), 50 % RDF + VC @ 5 t/ha (T8), 50 % RDF + FYM @ 10 t/ha + VC @ 5t/ha (T9), 75 % RDF + FYM @ 10 t/ha+ Azotobacter + PSB (T10), 75 % RDF + VC @ 5 t/ha+ Azotobacter + PSB (T11). The treatments were replicated thrice and were laid out in randomized block design (RBD). Wheat variety used for the experimentation was PBW 343. The net plot size was 5 m X 5 m and crop spacing was 20cm between rows. The results reported that wheat crop with the application of 75 % RDF + VC @ 5 t/ha+ Azotobacter + PSB (T11) performed better in terms of growth parameters and yield under field condition compared to some of the treatments. Significantly higher B: C (2.9) ratio was calculated for 75 % RDF + FYM @ 10 t/ha+ Azotobacter + PSB (T11). Percent increase of 5.5 % and 3.6 % was recorded during 2019-20 and 2020-21, respectively in grain yield with the application of 75 % RDF + VC @ 5 t/ha+ Azotobacter + PSB (T11) over 100 % RDF (T2). Maximum net returns were estimated in treatment T10 (75 % RDF + FYM @ 10 t/ha+ Azotobacter + PSB) of Rs. 97288 and Rs. 102005 followed by treatment T11 (75 % RDF + VC@ 5 t/ha+ Azotobacter + PSB) during both the years. Sowing on 20th November with GW 451 produced the highest returns and benefit-cost ratio. These results suggest that combined application of 75 % RDF + vermicompost @ 5 t/ha+ Azospirillum + PSB or 75 % RDF + FYM @ 10 t/ha+ Azotobacter + PSB is effective to get higher yield net return in wheat cultivation, providing valuable insights for farmers and researchers.

Effect of Different Sources of Nitrogen and Bio Fertilizers on Growth, Yield and Quality of Cherry Tomato [Solanum lycopersicum L.) Var. Cerasiforme] in Polyhouse Conditions

The present investigation was carried out at Research Farm, Department of Horticulture, Naini Agricultural Institute, SHUATS, Naini, Prayagraj, Uttar Pradesh during the Kharif-2022 with a view to identify the effect of different sources of nitrogen and bio fertilizers on growth, yield and quality of cherry tomato” [Solanum lycopersicum L.) var. cerasiforme] in polyhouse conditions. The experiment was laid in Randomized block design (RBD) with 9 treatments and 3 replications with different combination in RDF and application of organic nutrition. Under this experiment, overall, 10 treatment were taken. From the above experimental finding it may be concluded that the treatment T9 (75% N through urea + 25% N through FYM + Azotobacter (4 kg ha-1) + PSB (4 Kg ha-1) was found to be best in the terms of growth parameters like highest plant height (283.35 cm) at 120 DAT, maximum number of branches per plant (72.33 branches) at 120 DAT. In terms of earliness, it was found to have minimum days to attain 50% flowering (55.93 DAT). In terms of yield T9 had highest number of fruits per cluster (5.57 fruits), and fruit yield per hectare (25.88 t ha-1).

Control biológico de Ralstonia solanacearum y su efecto en el crecimiento vegetativo de banano orgánico

The banana (Musa AAA) is one of the main economically important crops worldwide. Currently, it faces a serious problem of plant death caused by the bacterium Ralstonia solanacearum (Smith) variety 2. The objective of this research was to evaluate the effect of biological control of Moko disease and its relationship with the vegetative growth of banana seedlings under field conditions. Four treatments were employed: absolute control (T0), Trichoderma spp. (T2), Bacillus spp. (Bio-remedy) (T3), and ADMF® (T4) with three replications in a completely randomized block design. The incubation period, the incidence percentage, and the area under the disease progress curve (AUDPC) were evaluated, along with plant survival and height, pseudostem diameter, and leaf emission rate. At 113 days after transplanting (dat), treatments T1, T2, and T3 showed higher seedling survival. Plant height (111-145 cm), pseudostem diameter (7.43-11.28 cm), and leaf emission rate (11.74-13.15 leaves) exhibited significant differences among treatments. Treated plants showed the lowest AUDPC (between 576.1 and 1435.4 units) compared to untreated plants (3156.55 units). Trichoderma, Bacillus, and ADMF® were demonstrated to reduce disease incidence and promote banana vegetative growth, proving to be viable sustainable alternatives for this crop. Keywords: Musa AAA, Trichoderma, Bacillus, ADMF®, Ralstonia solanacearum, AUDPC.

#2153 Difelikefalin: a new solution against itching in CKD-aP patients

Abstract Background and Aims Chronic kidney disease-associated pruritus (CKD-aP) is one of the most common and burdensome symptom affecting patients with advanced chronic kidney disease, with an estimated prevalence of 53.3%. The clinical presentation is extremely variable, from minor lesions to true scratch injuries, and significantly affects the patient's quality of life (QoL), sleep quality and mood, as well as being associated with increased mortality. At present, high-quality evidence on which to base treatment is limited. Currently proposed first-line therapeutic options include optimization of dialysis treatment, emollient agents, UVB light, antihistamines, gabapentin and pregabalin. Recently difelikefalin, a k-opioid receptor agonist, has been the first Food and Drug Administration (FDA) approved drug for moderate-severe CKD-aP treatment. The aim of our study is to demonstrate the efficacy of this drug in relieving symptoms in CKD-aP patients. Method For this preliminary retrospective observational study, 19 patients (9 females, 10 males) with CKD-aP performing dialysis at our Dialysis Center were recruited. All of them had appropriate Kt/V values and had previously been treated with first-line medications. The other main demographic and clinical characteristics of our population, such as calcium-phosphate metabolism and inflammation's indexes, can be seen in Table 1. Our population underwent the Worst Itch Numeric Rating Scale (WI-NRS) by which the presence, intensity, and impact on sleep of this symptom were investigated. They also were subjected to questions that investigate their quality of life (SF-PCS12, SF-MCS12). All patients completed those questionnaires before starting Difelikefalin treatment (t0) and after one month of treatment (t1), 15 of them were evaluated also after three months (t3) and 10 patients, those who first started therapy, reached the assessment at six months (t6). Results Statistical analysis of our preliminary study shows very encouraging data regarding the efficacy of difelikefalin on CKD-aP and sleep quality. As shown in Table 2, we recorded a significant decrease (p value &amp;lt;.001, ƞ2 0.552) in the intensity of itching, most noticeable in the first month, confirming that the effect of this drug is evident and considerable already after four weeks. Our data also demonstrate a significant and lasting positive effect of difelikefalin on sleep quality (p value 0.006, ƞ2 0.592), as shown in Table 3. We found no significant data on the improvement in the QoL of the patients in the study, probably due to the difficulty in standardising this complex feature and due to the limited size of the statistical sample. Furthermore, it should be considered that 3 of the patients enrolled in the study had to discontinue the medication due to the onset of side effects such as diarrhea and headache. Conclusion The most influential limitation of this retrospective observational study is the small number of recruited patients, in addition to the fact that not all of them reached a 6-months treatment evaluation. Our aim is to complete the study by enrolling a larger number of patients from our Dialysis Centre and to monitor the effect of difelikefalin during a longer treatment period, in order to achieve a more accurate analysis and significative results applicable on a larger scale. As far as we can currently evaluate, this drug is significantly effective in reducing CKD-aP and thus in improving the quality of sleep.