Palm Oil Mill Effluent Treatment Research Articles

Polymeric materials used for immobilization of microalgae for the bioremediation of palm oil mill effluent

The immobilization of microalgae on polymeric materials such as polyurethane foam (PUF) has shown distinct advantages over non-immobilized technique with significant pollutants degradation efficiency. However, the effect of volume and pore size of PUF on the microalgae performance to remove organics and ammonium has not been extensively investigated. In this study, a down flow hanging sponge (DHS) reactor packed with PUF carrier for the immobilization of microalgae was utilized in treating palm oil mill effluent. The result of chemical oxygen demand (COD) degradation indicates that smaller pore size (0.56 mm) and higher PUF volume (38.0 %) increased the total performance of DHS reactor. In addition, this study also found that the growth of microalgae was greatly influenced by different pore sizes and volumes of PUF.

Characterization and morphological study of microbes in treated palm oil mill effluents

Palm oil mill effluent (POME) is wastewater generated by palm oil milling. Due to its extremely polluting qualities, it must be treated before being discharged into the water course. This study was aimed to evaluate the bacterial growth of raw and treated POME as well as identifying indigenous microorganisms by determining the morphological characteristics of bacteria that were found in the POME. The bacterial growth was identified by bacterial enumeration of colony forming units (CFU). Besides, the morphological identification of bacteria was determined by using gram staining. The results show the best bacterial growth curve is from serial dilution factor of 10-6 with a total of 2.24 x 10-6 CFU/mL in raw POME and optimum growth on day seven. While for the treated POME, the total is 1.97 x 10-6 CFU/mL and recorded the optimum growth on day ten of incubation. The growth curve indicates the number of colonies in raw POME is higher than treated POME. It concluded that treated POME still has the bacteria although it has been treated. Apart from that, from the morphological identification by gram staining, the bacteria were Bacillus cereus, Bacillus subtilis, Staphylococcus aureus and Micrococcus luteus. From a gram staining, this research obtained all gram positive in purple colour from the POME samples. Two of them in treated POME were in Bacillus shape while the other two from raw and treated POME were in coccus shape, respectively.

Removal of Nutrients by Using Green Microalgae from Lab-Scale Treated Palm Oil Mill Effluent

The use of microalgae for the removal of contaminants such as total phosphorus (TP), total nitrogen (TN), chemical oxygen demand (COD), and other pollutants from palm oil mill effluent (POME) has recently received much attention. This study aimed to investigate the impact of POME as a nutrient on microalgal growth and the rate at which nutrients were removed. Three green microalgae species were isolated from Penang, Malaysia’s palm oil mill. The polyphasic approach, which combines morphological and molecular observations, was used for species identification. The three green microalgae were identified as Chlorella sorokiniana, Scenedesmus quadricauda, and Chlorococcum oleofaciens. All three green microalgae were cultivated in 25%, 50%, and 75% (v/v) of lab-scale palm oil mill effluent (LABT-POME) to investigate the growth of these three green microalgae in 100 mL of BBM. Under a light microscope and a scanning electron microscope (SEM), the morphological changes of those three green microalgae (before and after cultivation in 25%, 50%, and 75% dilution of (LABT-POME)) were observed. When cultivated in LABT-POME, C. oleofaciens showed the highest growth rate compared to the other two species of green microalgae. C. oleofaciens was able to remove more than 90% of total phosphorus (TP), total nitrogen (TN), and ammonia nitrogen from LABT-POME, as well as minimise soluble chemical oxygen demand (SCOD) by about 65%. The growth of C. oleofaciens was well fitted to the Verhulst growth kinetic model with an R2 value of 0.99 and a growth rate of 0.3195 day−1 (d−1). The results of this study show the ability of newly isolated green microalgae to remove nutrients (TP, TN, NH3–N, and SCOD) from POME, which could be used as an effective and environmentally friendly method to remove pollutants.

Structural Characterization of Polycrystalline Titania Nanoparticles on C. striata Biosilica for Photocatalytic POME Degradation.

The biosilica shell of marine diatoms has emerged as a unique matrix for photocatalysis, owing to its sophisticated architecture with hierarchical nanopores and large surface area. Although the deposition of titania nanoparticles on diatom biosilica has been demonstrated previously, their photocatalytic activity has been tested only for degradation of pure compounds, such as dyes, nitrogen oxide, and aldehydes. The efficiency of such photocatalysts for degradation of mixtures, for instance, industrial wastewaters, is yet to be investigated. Furthermore, reports on the lattice structures and orientation of nanotitania crystals on biosilica are considerably limited, especially for the underexplored tropical marine diatoms. Here, we report an extensive characterization of titania-loaded biosilica from the tropical Cyclotella striata diatom, starting from freshly grown cell cultures to photodegradation of wastewaters, namely, the palm oil mill effluent (POME). As Indonesia is the largest palm oil producer in the world, photocatalytic technology could serve as a sustainable alternative for local treatment of POME. In this study, we achieved a 54% loading of titania on C. striata TBI strain biosilica, as corroborated by XRF analyses, which was considerably high compared to previous studies. Through visualization using HR-TEM, supported by SAED and XRD analyses, nanocrystal TiO2 appeared to be trapped in an anatase phase with polycrystalline characteristics and distinct crystallographic orientations. Importantly, the presence of C. striata biosilica lowered the band gap of titania from 3.41 eV to around 3.2 eV upon deposition, enabling photodegradation of POME using a broad-range xenon lamp as the light source, mimicking the sunlight. Kinetic analyses revealed that POME degradation using the photocatalysts followed quasi-first-order kinetics, in which the highest titania content resulted in the highest photocatalytic activity (i.e., up to 47% decrease in chemical oxygen demand) and exhibited good photostability throughout the reaction cycles. Unraveling the structure and photoactivity of titania-biosilica catalysts allows transforming marine diatoms into functional materials for wastewater photodegradation.

Microwave physicochemical activation: an advanced approach to produce activated biochar for palm oil mill effluent treatment

Microwave physicochemical activation: an advanced approach to produce activated biochar for palm oil mill effluent treatment

Implementation of FeSO4·H2O as an Eco-Friendly Coagulant for the Elimination of Organic Pollutants from Tertiary Palm Oil Mill Effluent: Process Optimization, Kinetics, and Thermodynamics Studies

The biologically treated palm oil mill effluent (POME) urges further treatment to minimize the residual pollutant concentration for safe discharge in the nearest watercourse. In the present study, the post-treatment of biologically treated POME was conducted using ferrous sulfate monohydrate (FeSO4·H2O) as a coagulant. The influence of the FeSO4·H2O coagulation of POME was determined on the elimination of biochemical oxygen demand (BOD), suspended solids (SS), and chemical oxygen demand (COD) with varying flocculation time (min), slow mixing speed (rpm), coagulant doses (g/L) and pH. The FeSO4·H2O coagulation–flocculation experimental conditions were designed following the central composite design (CCD) of experiments and optimized by employing response surface methodology (RSM) based on the optimal SS, COD, and BOD elimination from POME. The maximum BOD, SS, and COD elimination achieved were about 96%, 97%, and 98%, respectively, at the optimized experimental condition. The surface morphology and elemental composition analyses of raw FeSO4·H2O and sludge generated after coagulation revealed that the FeSO4·H2O effectively removed the colloidal and suspended particles from POME. The well-fitted kinetic model equation was the pseudo-second-order kinetic equation to describe the FeSO4·H2O coagulation–flocculation behavior. The thermodynamics properties analyses revealed that the FeSO4·H2O coagulation of POME was non-spontaneous and endothermic. The residual SS, COD, and BOD in treated POME were determined to be 28.27 ± 5 mg/L, 147 ± 3 mg/L, and 6.36 ± 0.5 mg/L, respectively, lower the recommended discharged limits as reported by the Department of Environment (DoE), Malaysia.

Bioremediation strategies of palm oil mill effluent and landfill leachate using microalgae cultivation: An approach contributing towards environmental sustainability

Palm oil mill effluent (POME) is defined as the wastewater that contains high concentrations of organics, nutrients and oil and grease generated from the production process of palm oil. Therefore, proper discharge and management of POME is important to avoid deleterious impact on the environment. In fact, solid waste generation is a precursor for its disposal issues as most of the solid waste generated in developing nations is dumped into landfills. This has led to the threat posed by the generation of landfill leachate (LL). LL is a complex dark coloured liquid consisting of organic matter, inorganic substances, trace elements and xenobiotics. Hence, it is essential to effectively treat the landfill leachate before discharging it to avoid contamination of soil, surface & groundwater bodies. Conventional treatment methods comprises of physical, biological and chemical treatment, however, microalgal-based treatment could also be incorporated. Furthermore, with the benefits offered by microalgae in valorisation, the application of microalgae in POME and leachate treatment as well as biofuel production, is considerably viable. This paper provides an acumen of the microalgae-based treatment of POME and LL, integrated with biofuel production in a systematic and critical manner. The pollutants assimilation from wastewater and CO2 biosequestration are discussed for environmental protection. Cultivation systems for wastewater treatment with simultaneous biomass production and its valorisation, are summarised. The study aims to provide insight to industrial stakeholders on economically viable and environmentally sustainable treatment of wastewaters using microalgae, and eventually contributing to the circular bioeconomy and environmental sustainability.

Industry-driven mitigation measures can reduce GHG emissions of palm oil

Tropical peatland stores a large amount of carbon. In the last 20 years, drainage of Asian peat soil has increased to satisfy the demand of land for plantation agricultures. Industrial oil palm plantations occupy large areas of peatland in Indonesia and Malaysia, with associated GHG emissions and biodiversity loss, here referred to as nature occupation impact. This study performs a detailed Life Cycle Assessment (LCA) of 1 kg of palm oil for two case studies: PT SMART's Hanau and Sungai Rungau facilities in Central Kalimantan, Indonesia. The objective is to quantify the reduction in GHG emissions and nature occupation that has been achieved by implementing the following industry-driven measures: reducing the area of cultivated peat soil, reducing the peat drainage depth, and setting aside part of the land-bank for nature conservation. The results show that 1 kg of palm oil causes 2.72 and 2.25 kg CO 2 -eq./kg palm oil from Hanau and Sungai Rungau facilities respectively. These are 20%–34% lower than average RSPO certified palm oil and 49%–58% lower than average non-certified palm oil. Sungai Rungau achieves the reduction mainly due to a completely peat soil-free supply base. Hanau's peat emissions are instead 0.28 kg, compared to the 0.77 and 2.36 kg CO 2 -eq for RSPO certified and non-certified palm oil respectively, due to a very low drainage depth (18–25 cm compared to 57–73 cm in average of RSPO certified and non-certified respectively) and an overall lower share of oil palms on peat land. The impact on nature occupation is 24%–43% lower in Hanau and Sungai Rungau compared to non-certified oil and 4%–29% lower compared to RPSO certified respectively. About 8% of the total land bank of the Hanau supply-base has been set aside for nature conservation, reducing GHG emissions by 2% and nature occupation by 9%. Both Hanau and Sungai Rungau could also significantly reduce GHG emissions in the palm oil milling stage, by implementing biogas capture in palm oil mill effluent (POME) treatment. • Avoiding the cultivation of peat soil reduces the GHG emissions of palm oil production. • When oil palms are on peat soil, reducing the peat drainage depth reduces the GHG emissions. • Nature conservation initiatives reduce GHG emissions and nature occupation of palm oil production. • Capturing the biogas produced in palm oil mill effluents (POME) treatment reduces GHG emissions. • Using the captured biogas in boiler or biogas engine further abates the GHG emissions of palm oil.

Electrochemical oxidation of palm oil mill effluent using platinum as anode: Optimization using response surface methodology

This work investigates the electrochemical oxidation of palm oil mill effluent (POME) treatment using platinum (Pt) as anode and graphite as a cathode. The response surface methodology was used to investigate the relationships between different factors conditions (voltage, electrolysis time and chemical support) and responses of the treatment (chemical oxygen demand reduction, colour removal, and total oil removal). A quadratic mathematical model was chosen for all responses using Box-Behnken Design (BBD) with R2 0.9853 for COD reduction, R2 0.9478 for colour removal and R2 0.9185 for total oil removal. According to Derringer's function desirability, under the optimum condition (Voltage 15, electrolysis time 2 h, and 19.95 mg/L NaCl) of POME treatment, 84% of COD reduction, 98% of colour removal and 99% total oil of removal could be achieved. These results indicate that platinum as an anode material is effective for the electrochemical oxidation treatment of POME.

A critical analysis on biogas production and utilisation potential from palm oil mill effluent

The conventional method used for palm oil mill effluent (POME) treatment is associated with several environmental issues that affect the sustainable production of palm oil. Integrated POME treatment with a closed anaerobic digester is identified as a practical cleaner production approach to mitigate global issues on energy security and climate change and enhance the sustainability of the industry. This paper discusses the potential of capturing biogas to the palm oil industry and Malaysia's renewable energy (RE) and greenhouse gases (GHG) emissions targets. Approximately 75% GHG reduction could be achieved for every t of crude palm oil produced from palm oil mill with biogas capture. In total, 1750 million m3 biogas with GHG savings of 19.5 million t CO2eq can be achieved annually, if all palm oil mills in Malaysia capture the biogas. A 540 MW installed capacity or 37 million MMBtu in the form of bio-compressed natural gas (Bio-CNG) or biomethane, can supplement the nation's energy mix for the sustainable energy generation. These contribute towards enhancing the sustainability criteria and economic performance of the palm oil mills by adopting cleaner production technology of POME treatment for circular economy and better market access of palm oil products. Future work should emphasise on nationwide biogas implementation through synergising and strengthening private commitments and the government supports.

REMEDIATION OF PALM OIL MILL EFFLUENT (POME) USING SELECTED BIOLOGICAL TECHNIQUES: A MINI REVIEW

Undoubtedly, industrial activities have produced a large amount of waste, which has significantly contributed to the global environmental problem. The palm oil sector, which is considered a water-intensive industry, is one of the industrial sectors that have become a major source of severe water pollution concerns. Normally, 50% of the freshwater used during the extraction of crude palm oil will end up in the production of undesirable liquid waste. Thus, proper regulation and enforcement are required to control the discharge of such waste to ensure the sustainability of water resource management. Based on research databases such as Scopus, ScienceDirect, Web of Science and PubMed the total number of publications focused on palm oil mill effluent (POME) treatment has increased significantly over the past five years. The increase in publications shows the growing concern about the POME issue among the scientific community. This mini review focuses on selected biological treatment techniques for remediation of POME focusing on ponding systems, bioadsorption, activated sludge, microorganism and plant, aerobic biodegradation and integrated/hybrid treatment strategies. The summary of optimum operating conditions, characteristics of the treatment system, properties of materials and performance of each treatment are discussed in detail. To achieve a high level of contaminant removal performance, it is critical to discover the synergistic effect between the treatment procedures, materials employed, operational cost, and circumstances.

Performance of Membrane Bioreactor in Palm Oil Mill Effluent Treatment: An Overview

Malaysia is amongst the biggest palm oil manufacturer and exporter in the world associated with the high discharge of effluents. Membrane bioreactor (MBR) can be employed to treat the palm oil mill effluent (POME). However, data on MBR in treating POME is quite limited and could vary across different reported works. Thus, this study aims to provide some latest updates on the MBR technology in treating the POME. Based on the evaluation, separation of POME by using MBR can produce a good quality of effluent compared to conventional POME treatment. Mixed liquor suspended solids removal for a retention time of 11 days can be improved from 57.3% to 94.5 % using the MBR. According to analyses, when the MBR operating temperature falls into thermophilic region, the separation efficiency will start to decrease. Optimization of the MBR performance based on the operating temperature could be suggested in enhancing the overall MBR performance in treating POME.

Fouling studies on hydrophobic PVDF-bentonite hollow fiber membrane during membrane distillation of palm oil mill effluent

Membrane distillation (MD) is one of the emerging methods that can be adopted for oily wastewater treatment. However, the problems associated with membrane surface fouling may impede its separation efficiency. In this work, we performed a fouling study using self-synthesized polyvinylidene difluoride (PVDF)-bentonite hollow fiber membrane (HFM) to treat palm oil mill effluent (POME) via direct contact membrane distillation (DCMD). To date, there is no report on POME treatment using MD technology based on PVDF-bentonite HFM. The fouling tendency was identified based on the membrane's permeate flux and pollutant rejection. The fouled membranes after POME treatment were characterized based on its morphology, functional group, water contact angle, surface roughness and mechanical strength. The results showed that the average permeate flux of MD was 3.34 kg/m2·h over 72-h operation. The membrane showed good performance in separating pollutants with >95 % removal rate recorded for chemical oxygen demand, nitrate nitrogen, total suspended solid, total dissolved solid, color and turbidity. However, based on the post-characterization analysis, the used membranes still suffered from fouling after 72-h DCMD test. Thus, an in-depth investigation into the prevention and control of membrane fouling is still necessary for the POME treatment using DCMD.

Performance of Chlorella sp. and Multicultural Bacteria in Removing Pollutants from Nutrient-Rich Wastewater

The most common method of treating palm oil mill effluent (POME) is by using various types of bacteria communities. However, the utilization of microalgae in consuming the high nutrient content in wastewater offer additional benefit, particularly for CO2 sequestration. In this study, we proposed to evaluate the performance of multicultural bacteria obtained from municipal wastewater and Chlorella sp. for batch treatment of POME at different COD concentrations (ranges between 600 to 1,300 mg/L), microalgae species (C. vulgaris and C. pyrenoidosa) and speed of agitation (0 and 150 rpm). The results showed that between the bacteria and microalgae that are involved in POME treatment, microalgae give high removal of colour (93%) and ammoniacal nitrogen (95%). As for the COD and phosphate removal, both microorganisms show comparable performances. It was observed that C. pyrenoidosa was able to remove more colour compared to C. vulgaris where higher lipid yield production was obtained (47.6%). However, there is no significant impact of agitation on pollutant removal. This study also reveals that co-cultivation of different microalgae species does not affect the efficiency of the system. This study provides an important insight into developing an efficient and environmentally friendly method to treat wastewater by incorporating green technology in the treatment system

Reusability of Nano-Fe3O4/Polyvinylidene Difluoride Membrane for Palm Oil Mill Effluent Treatment

Membrane reusability determines the economic sustainability of separation process. However, the reusability of membrane was deduced by the antifouling properties, while the actual reuse performance of membrane using palm oil mill effluent (POME) is rarely reported. This study investigates the reusability of nano-Fe3O4/PVDF (PVDF1) membrane for POME treatment. The chemical oxygen demand (COD), total suspended solid (TSS) of permeate, molecular weight cut off (MWCO), pure water flux (PWF) and contact angle (CA) of membranes were also determined. Result showed that the neat membrane (PVDF0) and PVDF1 exhibited ~77°, ~342 L/(m2.h) and ~70°, 278 L/(m2.h) for CA and PWF, respectively, due to the small MWCO in PVDF1. The POME flux was maintained at ~16 L/(m2.h) in 5 cycles of filtration process. The COD rejection was higher for PVDF 1 compared to PVDF0, and PVDF1 showed consistent ~58 % COD and ~98 % TSS removal in all the cycles. The good performance of PVDF1 due to the presence of nano-Fe3O4, improved the membrane hydrophilicity and tailored the membrane pore size for POME treatment.
 HIGHLIGHTS
 
 Membrane reusability determines the economic sustainability of separation process
 Nano-Fe3O4 improves the hydrophilicity of PVDF membrane
 Nano-Fe3O4 tailors the pore size of PVDF membrane for POME treatment
 POME flux was maintained at ~16 L/(m2.h) for 5 cycles of filtration process
 The membrane can be reused for minimum 5 cycles with consistent COD and TSS removal
 
 GRAPHICAL ABSTRACT

Experimental investigation of biofilm carriers of varying shapes, sizes, and materials for wastewater treatment in fixed bed biofilm reactor: a qualitative study of biocarrier performance

AbstractBACKGROUNDBiofilm carriers were introduced in the early 1990s mainly to abate chemical oxygen demand (COD). However, studies have continued investigating few biocarriers repeatedly, without studying a large number of carriers in a single study under similar conditions.RESULTSThe current study investigated the performance of nine synthetic and eight natural biofilm carriers for 7 days of palm oil mill effluent (POME) treatment in terms of COD removal. A fixed bed biofilm reactor (FBBR) was 100% packed with biocarriers and operated at 35 °C. Performance based on the 7th day COD removal could be ranked in the following order: green ammonia absorption stone (91%), K1 micro (88%), coral sand (87.8%), volcanic stone (86.6%), Micro Media (85.5%), 1.6 cm BioBall (85%), pebble (82.4%), porous ceramic ring (82.3), K1 (82.1%), non‐porous ceramic ring (80.9%), brown ammonia absorption stone (78.6%), biological filter (77.7%), Ultra Media (76.8%), blue media (69.3%), 3.0 cm BioBall (68.8%), 2.5 cm BioBall (66.5%), and loofah sponge (63%).CONCLUSIONThe smallest carrier, K1 micro, yielded the highest COD removal (88%) on day 7 among the other synthetic carriers, while green ammonia absorption stones provided the highest quality effluent from day 1 (3565 mg COD L−1) to day 7 (780 mg COD L−1) and delivered the shortest start‐up compared with all natural and synthetic carriers. The discussion revealed that all carrier functionalities (i.e., scale, shape, surface pores, roughness, material, surface area, porosity, and durability) play a key role in deciding carrier quality. The current study also introduced ‘carrier efficiency limit’, which refers to the carrier's capacity to entrap biomass and immobilize microorganisms. © 2022 Society of Chemical Industry (SCI).

Recent Developments in Biological Processing Technology for Palm Oil Mill Effluent Treatment-A Review.

Simple SummaryPalm oil mill effluent (POME) requires treatment prior to discharge to the environment. Biological processing technology is highly preferable due to its advantages of environmentally friendliness, cost effectiveness, and practicality. These methods utilized various designs and modifications of bioreactors fostering effective fermentation technology in the presence of fungi, bacteria, microalgae, and a consortium of microorganisms. This review highlights the recent biological processing technology for POME treatment as a resource utilization.POME is the most voluminous waste generated from palm oil milling activities. The discharge of POME into the environment without any treatment processing could inflict an undesirable hazard to humans and the environment due to its high amount of toxins, organic, and inorganic materials. The treatment of POME prior to discharge into the environment is utmost required to protect the liability for human health and the environment. Biological treatments are preferable due to eco-friendly attributes that are technically and economically feasible. The goal of this review article is to highlight the current state of development in the biological processing technologies for POME treatment. These biological processing technologies are conducted in the presence of fungi, bacteria, microalgae, and a consortium of microorganisms. Numerous microbes are listed to identify the most efficient strain by monitoring the BOD, COD, working volume of the reactor, and treatment time. The most effective processing technology for POME treatment uses an upflow anaerobic sludge blanket reactor with the COD value of 99%, hydraulic retention time of 7.2 days, and a working volume of 4.7 litres. Biological processing technologies are mooted as an efficient and sustainable management practice of POME waste.

Performance Monitoring of Anaerobic Digestion at Various Organic Loading Rates of Commercial Malaysian Food Waste.

Application of anaerobic digestion (AD) has become common in treating palm oil mill effluent in Malaysia; however, employing AD in treating the organic fraction of municipal solid waste (OFMSW), especially food waste, is still scarce. This study aims to characterize the commercial Malaysian food waste (CMFW) and determine its potential as sustainable bioenergy feedstock through biogas production. The sample was digested via the biomethane potential (BMP) test with the variation of organic loading rates (OLRs), ranging from 0.38 to 3.83 gCOD/L. day, under mesophilic conditions. The digestion process was further evaluated in continuous operation using a 6-L continuous stirred-tank reactor (CSTR). The kinetic properties of the process were also determined. It was found that the CMFW had a significant amount of chemical oxygen demand of 230 g/L and an acidic pH of 4.5 with the carbon to nitrogen (C/N) ratio at 121:1. A maximum methane composition of 81% was obtained at 1.92 gCOD/L in the BMP test with specific methane production (SMP) at 0.952 L. CH4/L.COD fed. The biogas production was well-fitted with the modified Gompertz model with R 2 at 0.9983 and the maximum biogas potential production rate at Rm 0.1573 L/day, whereas in the CSTR operation, a maximum methane composition of 85% was produced at OLR 6 gCOD/L. day with the SMP of 1.13 L. CH4/L.COD fed. The CSTR system was in high stability as the pH was maintained in a range of 6.6–6.7, with an alkalinity ratio of 0.28. This study indicates the CMFW is a sustainable feedstock for biogas production in Malaysia. Toward a circular economy approach, the authorities shall introduce commercial scale CMFW AD as part of managing municipal solid waste issues in Malaysia.

Electrocoagulation treatment of raw palm oil mill effluent: Optimization process using high current application

In the electrocoagulation wastewater treatment process, extremely polluted water treatment requires an effective technique, and using high current is one of those. This study aims to optimize electrocoagulation parameters such as operation time, electrodes gap and the initial pH by applying high current intensity to treat palm oil mill effluent (POME) via Box–Behnken design (BBD) method. Chemical oxygen demand (COD), biological oxygen demand (BOD), and suspended solids (SS) were used as the response variables in the quadratic polynomial model. Most of the selected models in the analysis of variance (ANOVA) have shown significant results. A high connection between the parameters and dependent variables was surprisingly discovered in this study which the obtained value of R2 for removal percentage of COD, BOD and SS were 0.9975, 0.9984 and 0.9979 respectively. Optimal removal was achieved at 19.07 A of current intensity (equivalent to 542 mA/cm2 of current density), 44.97 min of treatment time, 8.60 mm of inter-electrode distance and 4.37 of pH value, resulted in 97.21%, 99.26% and 99.00% of COD, BOD and SS removal respectively. This optimized scheme of operating parameters combination offers an alternate choice for enhancing the treatment efficiency of POME and also can be a benchmark for other researchers to treat highly polluted wastewater.

Production of Fatty Acid Methyl Esther from Wastewater Grown Algae

Biodiesel is an alternative source of fuel that is non-toxic and biodegradable than conventional fossil-based fuel. In this research, biodiesel was produced by the cultivation of Chlorella sorokiniana in Palm Oil Mill Effluent (POME). The lipid content of the algae was obtained using the solvent extraction method. Gas-chromatography mass spectrometer was used for the identification of Fatty Acid Methyl Esther (FAME). Based on the results of biodiesel analysis, the components of FAME confirmed in microalgae biomass are mostly saturated fatty acid and they include tridecyclic acid (C13:0), myristic acid (C14:0), pentadecylic acid (C15:0), palmitic acid (C16:0) and stearic acid (C18:0). These indicated that the biomass of C. sorokiniana after POME treatment has the potential to be used for bioenergy production.