Mesopelagic fishes, which inhabit the Ocean Twilight Zone spanning ocean depths from 200-1000 m, are some of the most abundant vertebrates on earth, and are thought to play an important role in global ocean biogeochemical cycles. And yet there remain large uncertainties in their abundance, biomass, distribution, and behavior. Mesopelagic fishes are difficult to sample, or harvest for the purpose of commercial fisheries, due to their often remote and inhospitable habitats, and their ability to efficiently avoid trawl nets and cameras. As a result, there has been increased interest in using shipboard sonar systems to survey mesopelagic fishes, though little is known about their acoustic properties and target strengths. Recent advances in broadband acoustic scattering systems, post-processing analysis and approaches, and in situ platforms are being used to fill the technology gap and infer in situ target strengths of these elusive mesopelagic fishes. This talk presents an overview of the work done in this area to date, discusses the key challenges that still remain, and assesses the impact of broadband acoustic techniques in advancing our knowledge of the abundance, biomass, and distribution of mesopelagic fishes in the Ocean Twilight Zone. [Work supported by NSF and the WHOI OTZ Project.]