Traumatic Ulcer Healing Research Articles

Efficacy of silver nanoparticle gel on healing of traumatic oral ulcers compared with triamcinolone oral paste: An experimental study on rats.

There are many treatment protocols for relieving pain and accelerating oral ulcer healing. The purpose was to evaluate the anti-inflammatory and antimicrobial effect of silver nanoparticle (AgNP) gel on oral ulcers compared with the traditional treatment in animal model. In this experimental study, 72 adult male rats were randomly allocated into four groups, after ulcer induction: control (receive no treatment), placebo (placebo gel was applied on ulcers once daily), triamcinolone (ulcers were treated with triamcinolone paste once daily), and AgNPs (ulcers were treated with AgNP gel once daily). The animals were sacrificed at three successive periods of 3, 7, and 14 days after ulcer induction. Histopathological scores of re-epithelialization and degree of inflammation were assessed. The wound size was measured clinically. For parametric data, the analysis of variance, and for ordinal histopathological data, the Kruskal-Wallis test using SPSS was done. A P < 0.05 was considered statically significant. Control and placebo groups had no case of complete healing. Improvement of wound size by triamcinolone paste was not significant in different days but showed significant differences in degree of inflammation (P < 0.001) and re-epithelialization (P = 0.012). Reduction of wound size by AgNPs gel had significant difference at 14th day (P < 0.001); further, by this gel, significant differences in degree of inflammation and re-epithelialization were seen in long-term medication. AgNP gel could be effective in oral ulcer healing clinically and histopathologically. Although this gel showed delayed effect, it had better healing effect according to the cases with complete healing. Hence, the application of AgNP oral gel can be considered as an alternative approach for oral ulcers.

The Role of Rice Hull Liquid Smoke in the Traumatic Ulcer Healing.

Objective The aim of this study was to prove the role of rice hull liquid smoke (RH-LS) on lymphocytes, macrophages, fibroblasts, interleukin 6 (IL-6), and transforming growth factor β (TGF-β) expression during traumatic ulcer healing. Materials and Methods The RH-LS was obtained from the pyrolysis process. Traumatic ulcers were made 10 mm along the labial fornix incisive inferior of Wistar rat using a round stainless-steel blade. In control group, traumatic ulcers were treated using sterile water, and meanwhile in experimental group were treated using RH-LS once a day for 3, 5, and 7 days. After treatment, animal was terminated and their labial fornix incisive inferior tissues were biopsy and stained using hematoxylin and eosin staining to determine lymphocytes, macrophages, and fibroblasts. The IL-6 and TGF-β expressions were analyzed used immunohistochemistry staining. Result The lymphocytes, macrophages, and fibroblasts were higher in the RH-LS group for 3-, 5-, and 7-day treatment ( p < 0.05). The IL-6 expression was higher only in the 5-day treatment, and the TGF-β expression was higher in the 3- and 7-day treatment. Conclusion The RH-LS able to accelerated the traumatic ulcer healing by increasing the number of lymphocytes, macrophages, fibroblasts, IL-6, and TGF-β expression.

Evaluation of the Role of Propolis and a New Herbal Ointment in Promoting Healing of Traumatic Oral Ulcers: An Animal Experimental Study.

Most of the currently used medications for the treatment of oral ulcers focus primarily on providing symptomatic relief and preventing secondary infections. Not much attention has been paid in the past on agents that can promote healing of these lesions. In the current study, we have tested and compared the efficacy of such wound healing promoting agents i.e. Propolis (product obtained from Bees) and a newly developed herbal ointment (containing extracts of Azadirachta indica (Neem), Linum usitatissimum L.(Linseed) oil, and resin of Shorea robusta) in experimental rabbits.Methodology:Traumatic oral ulcers were inflicted in experimental rabbits (New Zealand white) with the help of a punch biopsy instrument. The animals were then divided into three groups each group consisted of 12 animals (n = 12), Group I was the untreated control group and Group II was topically treated with nonalcohol extract of propolis and Group III was treated with newly developed herbal ointment. Histological healing scores of the ulcers were evaluated on day 7 and day 14. Data were statistically analyzed using descriptive and inferential statistic methods.Results:In Group III treated with newly developed herbal ointment, significantly higher healing scores, in comparison to the untreated control group (Group I) and propolis (Group II) was observed on day 7 and 14. Improved healing scores in prapolis-treated group were observed than the untreated control group. However, the difference was not statistically significant on both day 7 and day 14.Conclusion:With the newly developed herbal ointment, superior healing of traumatic oral ulcers was observed.

The role of liquid smoke coconut shell in the proliferation phase of an oral traumatic ulcer

Context: Liquid smoke coconut shell (LS-CS) contains phenolic compounds that are able to promote wound healing by interfering with the inflammation phase of wound healing. The homeostatic mechanism that decreases the inflammation releases a growth factor that enhances proliferation by increasing fibroblast growth factor-2 (FGF-2), vascular endothelial growth factor (VEGF) expression and fibroblast proliferation. Aims: To analyze the role of LS-CS in the proliferation phase of oral traumatic ulcer healing by analyzing fibroblast numbers and FGF-2 and VEGF expressions. Methods: Oral traumatic ulcers were induced in diabetes mellitus Wistar rats via an alloxan injection. A traumatic ulcer as long as 10 mm was made in the inferior fornix incisive labial area. The oral traumatic ulcer was then topically treated with LS-CS using a dose of 20 μL/ 20 g body weight once daily for three, five and seven days. The fibroblast number of the oral traumatic ulcers’ tissue was then analyzed using hematoxylin-eosin, and the FGF-2 and VEGF expression were analyzed via immunohistochemistry staining. The difference in fibroblast numbers and FGF-2 and VEGF expressions were analyzed by one-way ANOVA and post hoc tests with a significance of p&lt;0.05. Results: The LS-CS affected fibroblast numbers (p=0.001) and FGF-2 expression (p=0.000) after topical treatment for seven days. There was a significant difference in the expression of VEGF after topical treatment with LS-CS for five days compared to the control group (p=0.000) and benzydamine hydrochloride (p=0.005). The topical treatment of LS-CS for seven days affected the expression of VEGF compared to the control group (p=0.000) and benzydamine hydrochloride (p=0.019). Conclusions: LS-CS could improve the healing of oral traumatic ulcers by increasing fibroblast numbers and FGF-2 and VEGF expression after seven days of treatment.

Effectiveness of gold sea cucumber (Stichopus hermanii) extracts in accelerating the healing process of oral traumatic ulcer in rats

Introduction: Gold sea cucumber (Stichopus hermanii) is a marine organism that contains omega-3 and glycosaminoglycan for enhancing collagen needed in the healing process. This study was aimed to determine the effectiveness of gold sea cucumber extracts in accelerating the healing process of oral traumatic ulcer in Wistar rats, in terms of the correlation between collagen type I expression, ulcer diameter, and body weight loss. Methods: Twenty Wistar rats (Rattus norvegicus) was divided into four groups and treated with gold sea cucumber extracts with the concentration of 80%, 40%, and 20% in the form of 0.1 ml gel daily. The negative control group received no treatment. Ulceration of the lower lip of labial mucosa was induced by a burnisher. The animals were observed for 7 days, during which they were weighed and the ulcers diameter was measured. The rats were then sacrificed after 7 days of treatment. Histometric analysis of collagen type I expression was also performed. Data obtained were analysed for differences between the group with one-way ANOVA test and the correlation was analysed with the Pearson test. Results: The significant differences (p &lt; 0.05) between treatment groups was found in the ulcer diameters and the collagen type I expression. The most decreased ulcer diameter and the highest collagen type I expression detected in the group of treatment with a concentration of 80% — no significant differences found in the weight loss between-groups (p &gt; 0.05). Strong correlation was also found between the expression of collagen type I and diameter of ulcer (Pearson correlation = 0.599, p = 0.05), however, no correlation found between weight loss with the expression of collagen type I (Pearson correlation = -0.102, p = 0.66), and the ulcer diameter (Pearson correlation = 0.022, p = 0.92). Conclusion: Gold sea cucumber had a potential benefit to be used as a traumatic ulcer healing medicament. Water extract of gold sea cucumber able to accelerate traumatic ulcer healing by enhancing the production of collagen type I and reduce the diameter of ulcer. There is a strong correlation between collagen type 1 expression and diameter of ulcer, and a weak correlation found between body weight loss and collagen type I expression, also with reducing ulcer diameter.Keywords: Gold sea cucumber, Stichopus hermanii, healing process, traumatic ulcer.

The effects of topical application of red pomegranate (Punica granatum Linn) extract gel on the healing process of traumatic ulcers in Wistar rats

Background: Trauma-induced ulcers constitute one of the painful abnormalities affecting the oral cavity about which numerous individuals complain. The prevalence of traumatic ulcers (15-30% of cases) is relatively high compared to other oral lesions. Unfortunately, the use of anti-inflammatory drugs classified as steroids can lead to contraindications and serious side effects. Hence, the use of natural ingredients represents an alternative treatment. One such ingredient is red pomegranate containing bioactive elements acting as anti-inflammatory, antibacterial, antimicrobial, and antioxidant agents which accelerate the healing process in traumatic ulcers. Purpose: The aim of this study was to determine the effects of the topical application of red pomegranate (Punica granatum Linn) extract on the healing process in traumatic ulcers in Wistar rats. Methods: 24 male rats, aged 2-3 months, were divided into six groups, namely; two control groups (PC: triamcinolone acetonide and NC: sterile aquades) and four treatment groups (red pomegranate extract gel at respective concentrations of 12.5%, 25%, 50% and 75%). A traumatic ulcer was subsequently created using a flame-shaped burnisher tip 2mm in diameter and heated for ±15 seconds. The diameter traumatic ulcer of each research subject was measured and observed until it healed within the remedial parameters determined by means of a UNC-15 periodontal probe which had been placed on it. The data obtained was analyzed using Kruskal-Wallis and Mann-Whitney tests. Results: Topically applied red pomegranate extract gel can reduce the width of the ulcerated area, while also accelerating traumatic ulcer healing. There was a significant difference in the healing time between the group using red pomegranate extract gel at a concentration of 75% and the positive control group using 0.1% triamcinolone acetonide with p=0.44 (p&lt;0.05). Conclusion: Red pomegranate extract gel possesses the ability to accelerate the healing process in traumatic ulcers. The most effective concentration of red pomegranate extract gel at accelerating the healing process in traumatic ulcers is 75%.

Potential of Distilled Liquid Smoke Derived from Coconut (Cocos nucifera L) Shell for Traumatic Ulcer Healing in Diabetic Rats.

Objective Distilled liquid smoke (DLS) is a result of coconut processing by-product that not only serves as a natural food preservative but also has a promising therapeutic effect. The healing potential of DLS derived from coconut ( Cocos nucifera L) shell was investigated on a traumatic ulcer with the diabetic rat. Materials and Methods DLS was analyzed the component by gas chromatograph mass spectrometry. Diabetic condition was induced by alloxan in 55 male Wistar rats. Ten mm of traumatic ulcer was made along the labial fornix incisive inferior after the diabetic condition was confirmed. Then DLS coconut shell, benzydamine hydrochloride, and sterile distilled water were applied topically for 3, 5, and 7 days. The potential healing was evaluated based on the expression of nuclear factor kappa beta (NFκB) and tumor necrosis factor alpha (TNF-α) on macrophages using immunohistochemical staining and the amount of collagen using Masson Trichome staining. The difference between each group was analyzed using one-way analysis of variance. The least significant difference test is used to determine the significant difference ( p < 0.05). Results The major compounds found were phenol (36.6%), 2-methoxyphenol (guaiacol) (25.2%), furfural (17.8%), and 4-ethyl-2-methoxyphenol (3.5%) with 28 other minor constituents. The lowest NFκB and TNF-α expression on macrophage was observed by topical application of DLS derived from coconut shell for 3, 5, and 7 days of treatment. The amount of collagen was increased and indicated by the highest result of DLS compared to others. Conclusion The DLS derived from coconut ( Cocos nucifera L) shell was able to improve traumatic ulcer healing in a person with diabetes.

Expression of FGF-2 and Fibronectin in Citrus limon Fruit Peel Malang Essential Oil Gel Treated Traumatic Ulcer in Diabetic Wistar Rats (Rattus novergicus)

Diabetes Mellitus is a metabolic disease that delayed traumatic ulcer healing process. Citrus limon contains fumaric acid, d-limonene and citral that can accelerate the healing of traumatic ulcer. The aims of this study were to investigate the effect of 0.78% Citrus limon essential oil topical application can increase Fibroblast Growth Factor-2 (FGF-2) and Fibronectin expression. This study was true experimental analytic study with random sampling methods. The study was conducted on 30 male Wistar rats (Rattus novergicus) induced with DM by injecting 50 mg/kg of Streptozotocin, intraperitoneally, and a traumatic ulcer on their lower lip mucosa. These were divided into six groups; three each for control and treatment groups. Each control and treatment group consisted of five rats. The control groups treated with CMC 5% gel and treatment groups were administered with Citrus limon peel essential oil gel. The expression of FGF-2 and Fibronectin was observed on Days 5, 7 and 9. Furthermore, mice sacrificed and the lower lip labial mucosa tissue of mice has been taken to make the Histopathology Anatomy preparation by means of immunohistochemical examination with monoclonal antibodies anti-FGF2 and anti-Fibronectin. ANOVA analysis was performed to analyze the difference between group (p<0.05). The increased FGF-2 and Fibronectin expression in the treatment group as compared to that of the control group. There were significant differences (p<0.05) of both FGF2 and Fibronectin expression between the two groups. The citrus limon peel essential oil gel increased the FGF-2 and Fibronectin expression during the healing process of traumatic ulcers on the oral mucosa of diabetes afflicted Wistar rats (Rattus novergicus).

The effect of musa acuminata stem in increasing macrophage and neovascular cells of healing process

Introduction: Musa acuminata stem has both of imunostimulatory and imunosuppressant effects that are influenced by their concentrations. The study aimed to analyze the effect of Mauli banana stem extract (EMBS) to increase the number of macrophage and neovascular cells in traumatic ulcer healing. Methods: Rattus norvegicus of male Wistar strain were used as model of traumatic ulcer. The left buccal mucous was biopsied, then used the histopathology method to find the number of macrophage and neovascular cells. Results: EMBS gel was effected when administered at a concentration of 37.5% because it significantly increased the number of macrophage and neovascular cells in traumatic ulcer healing on the 3rd day to 5th days. Conclusion: It can be concluded that EMBS within concentration of 37.5% can accelerate ulcer healing through the increasing number of macrophage and neovascular cells.

Mauli banana stem extract application increased expression of NF-κB in traumatic ulcer healing

Background: A traumatic ulcer represents one of the most prevalent disorders affecting the oral cavity. Ulceration of the oral cavity potentially results in secondary infection requiring topical medication which involves the use of antiseptics to accelerate wound healing. Previous research has shown that Mauli banana (Musa acuminata) stem extract (MBSE) contains bioactive material from terpenoid saponin present in Ambon bananas. The terpenoid saponin in Ambon banana stems will be captured by a G protein receptor in the macrophages, subsequently producing a protein kinase C that activates nuclear factor kappa beta (NF-κB). This increases both the activity and number of macrophages. Purpose: To analyze the expression of NF-κB (p50) in traumatic ulcers as an effect of MBSE. Methods: A true experimental design with a post-test only control group. It involved 40 male Rattus norvegicus strain rats as traumatic ulcer models divided into four groups: the negative control group administered gel, and the other treatment groups administered 25%, 37.5% and 50% ethanol extracts of MBSE gel respectively. A biopsy was performed on days 3 and 5. The preparation was produced to analyze the expression of NF-κB (p50) by means of immunohistochemistry examination. Results: There was a significant difference (p&lt;0.05) in NF-κB (p50) expression (p=0.005) following MBSE gel administration of 37.5% concentration on day 3 compared to day 5. Conclusion: It can be concluded that MBSE gel topical application can increase expression of NF-κB (p50) in traumatic ulcer healing.

THE EFFECT OF MAULI BANANA (Musa acuminata) STEM EXTRACT GEL APPLICATION WITH 37.5% CONCENTRATION ON FIBROBLAST CELL COUNT (In Vivo Study On Wound Healing Process of Male Wistar Rat (Rattus norvegicus) Buccal Mucosa)

Background: Wound healing is the body process to improve tissue intergrity caused by wound. Mauli banana stem is a potential plant which can be used as a medicine to accelerate wound healing. Mauli banana stem extract has some contents such as tannin and saponin which has immunomodulatory properties. Previous study states that mauli banana stem extract gel with 37,5% concentration can accelerate the healing of traumatic ulcers. Purpose: To analyze the effect of mauli banana stem extract gel application with 37,5% concentration on fibroblast cell count in wound healing process of oral mucosa wistar rat on the 7th day. Material and Methods: This study was a true experimental design with posttest only control group design. It consisted of three treatment groups: a group which given mauli banana (Musa acuminata) stem extract gel with 37,5% concentration, hydroxypropyl methylcellulose gel (negative group) and patent drug gel containing Aloe vera (positive group). Result: The mean value of fibroblast cell count in wound healing process on the 7th day of the treatment group (28,57), negative group (20,14), and positive group (23). One-way Anova’s test had shown a significant difference. Post Hoc LSD test showed a significant difference between mauli banana stem extract gel with 37,5% concentration with hydroxypropyl methylcellulose gel and patent drug gel containing Aloe vera. Conclusion: The application of mauli banana stem extract gel with 37,5% concentration can increase fibroblast cell count on the 7th day in wound healing process of oral mucosa.

Effects of topical application of propolis extract on fibroblast growth factor-2 and fibroblast expression in the traumatic ulcers of diabetic Rattus norvegicus.

Background:A traumatic ulcer caused by diabetes mellitus (DM) is a lesion caused by an increase in advanced glycosylation end products (AGEs), which takes a long time to heal. AGEs cause angiogenesis, vasculogenesis and a decrease in leukocytes. Fibroblast proliferation and the number of glycosaminoglycans decline, thereby inhibiting the formation of granulation tissue, collagen deposition and platelet derivatives growth factor. The application of topical propolis extract gel to ulcers has an anti-inflammatory function, triggers angiogenesis and accelerates wound healing.Aims:This study sought to establish whether the topical application of propolis extract gel can increase the expression of fibroblast growth factor-2 (FGF-2) and fibroblasts in the healing process of traumatic ulceration in diabetic Wistar rats (Rattus norvegicus).Methods:This was a genuinely experimental research design featuring posttest-only control groups. The simple random sampling technique involved 24 male DM Wistar rats with traumatic ulcers on the labial mucosa of the lower lip. The samples were divided into two groups: a control group whose members were administered hydroxypropyl methylcellulose gel 5% and a treatment group to which propolis extract gel was applied. The expression of FGF-2 and fibroblasts was observed on days 3, 5, 7 and 9 by means of histology and immunohistochemistry (hypothalamic-pituitary-adrenal) with Ab-Mo FGF-2.Results:The topical application of propolis extract gel increased the expression of FGF-2 and fibroblasts in the treatment group on days 5 and 7. There was a correlation between the increased expression of FGF-2 and the number of fibroblasts (P < 0.05).Conclusion:The topical application of propolis extract gel increases the expression of FGF-2 and fibroblasts within the traumatic ulcer healing process in diabetic R. norvegicus.

Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation

ObjectiveTo evaluate the influence of overexpression HGF on the healing of traumatic ulcer of oral mucosa of mice. Material and methodsMice were divided into two groups: wild type C57BL6(WT) and HGF high expression transgenic (HGF-Tg) mice. Traumatic ulcer of all mice were made by number 15 scalpel blade. Mice were sacrificed after 5days and the inflammation score and expression of TNFα, IFNγ, c-Met, apoptosis (TUNEL) and 40 serum inflammation cytokines were estimated. ResultsHGF-Tg mice presented a lower inflammation score (p=0.011), Serum TNFα expression in HGF-Tg ulcers is 1.3 times than WT ulcer and the difference is statistical significance (t test, p=0.003). Serum c-Met protein in HGF-Tg mice were significantly higher than WT mice (t test, p=0.004). No statistical difference was observed in the serum IFNγ between WT ulcer and HGF-Tg ulcer (t test, p=0.268). TNFα positive cytoplasm expression cells in connective tissue of HGF-Tg mice is significantly lower than that of WT group (t test, p=0.029). C-Met positive cytoplasm expression cells in both epithelium and connective tissue of HGF-Tg group is significantly higher than that of WT group (t test, p=0.040, p=0.000). Samples in HGF-Tg group showed a lower number of positive cells of epithelium TUNEL staining compared with that in the WT group (t test, p=0.035). ConclusionsHGF exhibited anti-inflammatory potential in oral traumatic ulcer through the reduction of epithelial apoptosis, connective tissue TNFα expression and induction of c-Met expression.

Analysis of traumatic ulcer healing time under the treatment of the Mauli banana (Musa acuminata) 25% stem extract gel

ABSTRACTIntroduction: Traumatic ulcer is one of oral disorders that often occur. The prevalence is quite high, between 3-24% of population. Therapy of traumatic ulcer is symptomatic. People usually use Aloe vera extract gel. Previous research showed that the Aloe vera gel is equivalent with ethanol extract of 25% Mauli banana (Musa acuminata) stem in accelerating wound healing based on the number of macrophages and neovascular. The objective of the study is to find out the time difference of traumatic ulcer healing using 25% Musa acuminata stem extract gel compared to a gel containing Aloe vera extract. Methods: The research was post test design. Subjects were patients of Oral Medicine Installation in Gusti Hasan Aman Dental Hospital Banjarmasin with diagnosis of traumatic ulcer using completely random sampling method. Eight patients as samples were determined by the formula of Lemeshow. Results: there was no significant difference with p=0,724 (p&gt;0,05) between healing time using Aloe vera gel and 25% Musa acuminata stem extract. Conclusion: At the concentration of 25% Musa acuminata stem extract gel concentration has the same healing time with Aloe vera gel, therefore can be recommended as a topical use for traumatic ulcer healing.Keywords: Musa acuminata stem, Healing time, Traumatic ulcer

Effect of G-CSF on oral mucositis and traumatic ulcers produced in the tongue of rats undergoing radiotherapy: clinical and histologic evaluation

To investigate the effect of granulocyte colony-stimulating factor (G-CSF) on oral mucositis (OM) and on the healing of traumatic ulcers produced in the tongue of rats undergoing radiotherapy (RT). Twenty-seven Wistar rats were assigned to four groups: (1) RT+ traumatic ulcer+ filgrastim (G-CSF analog; n=7); (2) RT+traumatic ulcer+ saline (n= 7); (3) no RT+ traumatic ulcer+ filgrastim (n= 7); and (4) no RT+ traumatic ulcer (n= 6). The radiation dose was 30 Gy, and medication was filgrastim (10 μg/kg) for 7 days. Clinically, groups differed in the presence (Fisher's exact test: P= .008) and size of traumatic ulcers after irradiation (Kruskal-Wallis test: P= .032) and in the severity of OM (Fisher's exact test: P= .005 between the irradiated groups). Histologically, there was an increased inflammatory response in the nonirradiated groups (Fisher's exact test: P= .001). Filgrastim reduced manifestations and the severity of trauma-induced ulcers and radiation-induced OM. Significant differences were not observed histologically between the study drug and respective control groups.

TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats.

ABSTRACTDiabetes mellitus (DM) is a disease associated with delayed wound healing of oral ulcers by increased expression of proinflammatory cytokines and cellular apoptosis. Objective to evaluate the influence of Tumor Necrosis Factor alpha (TNF-α) and apoptosis in rats with DM treated with chamomile extract or triamcinolone.Material and Methods Wistar male rats (210.0±4.2 g) were divided into five groups: negative control group (NCG) without diabetes; positive control group (PCG) with DM (alloxan, 45 mg/kg); and groups treated with chamomile extract (normoglycemic= NCG group and diabetic= DCG group) and with triamcinolone (TG). Traumatic ulcers were performed on all animals that received topical triamcinolone, chamomile extract or saline 12/12 hours for ten days.Results On days five and ten the animals were euthanized and the ulcers were analyzed by light microscopy, TUNEL assay, and immunohistochemically (TNF-α). The NCG (p=0.0062), PCG (p=0.0285), NCG (p=0.0041), and DCG (p<0.0001) groups were completely healed on the 10th day, however, there was no healing on the TG (p=0.5127) group. The TNF-α expression showed a significant reduction from the 5th to the 10th day in NCG (p=0.0266) and DCG (p=0.0062). In connective tissue, the TUNEL assay showed a significant reduction in the number of positive cells in NCG (p=0.0273) and CNG (p=0.0469) and in the epithelium only in CDG (p=0.0320).Conclusions Chamomile extract can optimize the healing of traumatic oral ulcers in diabetic rats through the reduction of apoptosis in the epithelium and TNF-α expression.

Experimental Confirmation of the Utility of Nasturtium officinale Used Empirically as Mouth Lesion Repairing Promotor

Nasturtium officinale R.Br. (Brassicaceae), “watercress”, is an herbal plant widely consumed as food by many cultures. Along with this, it is used in folk medicine to help in treating asthma, bronchitis, hypertension, as well as other diseases. Rural Italian communities have used N. officinale as an adjuvant to cure bovine stomatitis without any scientific evidence that supports its use for such end. This study evaluated the efficacy of N. officinale as an adjuvant on the healing of traumatic ulcers on rat tongue dorsum. Method: In four control-experiment group-pairs (eight animals per group) there were punch induced 0.5 mm lesions on tongue dorsum, to mimic traumatic ulcers. Rats from control groups received saline solution onto lesions daily. Animals from experimental groups received oral solution containing 15% N. officinale ethanolic extract onto lesions. Animals were euthanized at 2, 7, 14, and 21 days after lesion induction. Lesions were qualitatively evaluated by hematoxilin-eosine stain, and quantitatively, by picrosirius red stain under polarized light. Results: There were observed higher deposits of total, immature and mature collagen in experimental groups than its respective control groups after 14 and 21 days. Conclusion: Results revealed that the solution containing 15% N. officinale extract promoted wound healing on rats tongue dorsum with higher collagen deposits.

Effectiveness of a novel topical powder on the treatment of traumatic oral ulcers in orthodontic patients: A randomized controlled trial.

To determine if 2-DeNT Oral Topical Powder is an effective treatment for traumatic oral ulcers. Of the 46 patients who were randomly allocated, 20 patients from the experimental group and 17 from the placebo control group completed the study. The patients, operators, and evaluators were all blinded. Patients applied the powder twice a day and completed a diary twice a day for 10 days. The diary was used to monitor the size of the lesions and pain levels (using a 10-cm visual analog scale). By day 5, the ulcers in the experimental group had reduced in size by approximately 70%; and ulcers in the control group had reduced in size by 56%. The experimental-group ulcers were significantly (P < .05) smaller than the control-group ulcers from day 5 through day 9. Ulcers in the experimental group were completely resolved by day 8, whereas control-group ulcers were still present on day 10. Patients experienced a significant amount of stimulated pain until the night of day 2 in the experimental group and until the night of day 5 in the control group, but group differences in pain were not statistically significant. The 2DeNT Oral Topical powder was more effective than the placebo powder at accelerating the healing of oral traumatic ulcers.

Ekstrak daun mangrove (A.marina)mempercepat kesembuhanulkus traumatikus (Extract of mangrove leaf (A.marina) accelerates the healing of traumatic ulcer)

Mangrove leaf (Avicennia marina) has a role in wound healing process, but its effect in wound healing in oral mucosa has not been researched yet. This study aimed to prove the effect of A.marina extract against traumatic ulcer and itseffective concentration in traumatic ulcer healing. The subjects of this research are 25 wistar rats that were randomized into 5 different groups; K0 control, K1 were given hyaluronic acid, P1 were given extract of A.marina10%, P2 were given extract of A.marina 20%, and P3 were given extract of A.marina 40%. After the subjects werewounded using amalgam stopper that has been heated before, they were given topical application once daily untilseven days. The ulcer diameter was measured at the second day and day 8 using caliper digital. The data, analyzedusing Kruskal-Wallis test showed that there’s difference in diameters between two groups (p&lt;0.05). The averagediameter differences of traumatic ulcer among rats are K0 = 0.5700 mm, K1 = 0.8380 mm, P1 = 0.7240 mm, P2 =0.8440 mm, and P3 = 0.9500 mm. It was concluded that A.marina extract of 10%, 20% and 40% concentrations haveeffect in the healing of traumatic ulcer, and 40% is the most effective concentration against traumatic ulcer.