Transverse Blood Flow Research Articles

Visualization of two-dimensional transverse blood flow direction using optical coherence tomography angiography.

.Significance: Evaluation of vessel patency and blood flow direction is important in various medical situations, including diagnosis and monitoring of ischemic diseases, and image-guided vascular surgeries. While optical coherence tomography angiography (OCTA) is the most widely used functional extension of optical coherence tomography that visualizes three-dimensional vasculature, inability to provide information of blood flow direction is one of its limitations.Aim: We demonstrate two-dimensional (2D) transverse blood flow direction imaging in en face OCTA.Approach: A series of triangular beam scans for the fast axis was implemented in the horizontal direction for the first volume scan and in the vertical direction for the following volume scan, and the inter A-line OCTA was performed for the blood flow direction imaging while the stepwise pattern was used for each slow axis scan. The decorrelation differences between the forward and the backward inter A-line OCTA were calculated for the horizontal and the vertical fast axis scans, and the ratio of the horizontal and the vertical decorrelation differences was utilized to show the 2D transverse flow direction information.Results: OCTA flow direction imaging was verified using flow phantoms with various flow orientations and speeds, and we identified the flow speed range relative to the scan speed for reliable flow direction measurement. We demonstrated the visualization of 2D transverse blood flow orientations in mouse brain vascular networks in vivo.Conclusions: The proposed OCTA imaging technique that provides information of 2D transverse flow direction can be utilized in various clinical applications and preclinical studies.

Adaptive transverse blood velocity estimation in medical ultrasound: A simulation study

Undoubtedly, highly valuable information about vascular anomalies is attained by the examination of the blood flow profile. The chief drawback of the conventional medical ultrasound in preparation of the blood periodogram is the measurement system shortcoming at the beam to flow angles near 90°. Recently, a method based on transverse oscillation (TO) approach, known as “Fourth-order estimation”, has been developed to directly estimate the transverse power spectral density (PSD) of the fully transverse blood flow. One of the basic requirements to accomplish acceptable PSDs by this technique is the sufficiently large observation window. In this paper, two adaptive approaches for efficient estimation of the velocity spectrum of a fully transverse flow by a limited observation window length are described. The first proposed adaptive approach is based on the minimum variance adaptive spectral estimation in combination with the well-known TO technique (TO-MV). Then, by exploiting the eigenspace separation of the observed data to eliminate the contribution of the undesired components, the second technique (TO-EIBMV) is developed. The approaches are validated using Field II simulations for pulsating flow. The proposed methods are tested and compared to the conventional TO transverse spectral estimator by metrics of relative standard deviation (RSD) and relative bias (RB). One of the main achievements is the decrement of the required data samples for spectrogram estimation, which leads to a better temporal resolution. Moreover, for the analyzed adaptive techniques, the robustness of the estimation results for the beam to flow angles of 60–90° and vessel depths ranging from 20 mm to 60 mm are investigated.

All-optical photoacoustic Doppler transverse blood flow imaging.

The method of measuring blood flow in photoacoustic microscopy usually relies on ultrasonic transducers in contact fashion, which is not favored in many applications, such as wound areas, burns, and anabrosis. Here we present a noncontact photoacoustic velocity measurement method to quantitatively map transverse blood flow based on the photoacoustic Doppler (PAD) bandwidth broadening method with an all-optical photoacoustic microscopy system. It is validated that the PAD bandwidth broadening is proportional to the transverse flow within a certain range. The transverse flow speed ranging from 0 to 5.5mm/s, as well as sectional flow images, was obtained in the blood-mimicking flow phantoms. Furthermore, the blood flow image of the mouse ear demonstrates that the all-optical photoacoustic Doppler method can acquire the information of blood flow in vivo, which could significantly broaden the scope of applications for obtaining the blood flow velocity of the microvasculature in biomedicine.

Wide-field absolute transverse blood flow velocity mapping in vessel centerline.

We propose a wide-field absolute transverse blood flow velocity measurement method in vessel centerline based on absorption intensity fluctuation modulation effect. The difference between the light absorption capacities of red blood cells and background tissue under low-coherence illumination is utilized to realize the instantaneous and average wide-field optical angiography images. The absolute fuzzy connection algorithm is used for vessel centerline extraction from the average wide-field optical angiography. The absolute transverse velocity in the vessel centerline is then measured by a cross-correlation analysis according to instantaneous modulation depth signal. The proposed method promises to contribute to the treatment of diseases, such as those related to anemia or thrombosis.

In vivo photoacoustic imaging of transverse blood flow by using Doppler broadening of bandwidth

A method is proposed to measure transverse blood flow by using photoacoustic Doppler broadening of bandwidth. By measuring bovine blood flowing through a plastic tube, the linear dependence of the broadening on the flow speed was validated. The blood flow of the microvasculature in a mouse ear and a chicken embryo (stage 16) was also studied.

Effects of aggregation of red cells and linear velocity gradients on the correlation-based method for quantitative IVUS blood flow at 20 mhz

Recent computer simulations suggest that the presence of aggregates of red blood cells (RBCs), at random angles and lengths, does not affect the measurements of blood flow transverse to the ultrasound (US) beam direction using a correlation-based method and an intravascular (IV) US array catheter. However, in case of aggregates of RBCs aligned with the flow, measurements of simulated blood velocity are affected. Blood velocity gradients were also shown not to influence the correlation-based method for blood velocity estimation. The objective of this study was to quantify the influence of aggregates of RBCs and blood velocity gradients on the correlation-based method during in vitro experiments. For this purpose, measurements were performed on washed RBCs (no aggregation), normal human blood, and two types of diseased blood in which a lower or a higher level of aggregation was present. The decorrelation pattern of a circular US transducer as a function of transverse blood flow was studied using a Couette system. Changing the shear rate of the Couette system modified the aggregation level of RBCs and the velocity gradient. With the exception of the results at low shear rates and abnormally high aggregation levels, agreements were found between the autoconvolution of the acoustical beam (reference curve) and the radiofrequency (RF) decorrelation patterns. For the high shear rate present in coronary arteries, the correlation-based method for blood flow estimation should not be influenced by these phenomena. (E-mail: a.vandersteen@erasmusmc.nl)

Quantitative IVUS blood flow: validation in vitro, in animals and in patients

In recent years, a new method to measure transverse blood flow based on the decorrelation of the radiofrequency (RF) signals of intravascular ultrasound (IVUS) rotating single-element scanners was introduced. We report here in vitro, animal and patient testing to evaluate the correlation-based method using an IVUS array catheter. A new correlation-based method to dynamically correct the correlation coefficients for noise is implemented. The decorrelation due to noise was estimated from the correlation coefficients from flowing blood obtained at increasing time lags. First, blood flow experiments were carried out with different catheters in a tissue-mimicking flow phantom with an inner diameter ranging from 3.0 to 5.0 mm. A calibrated electromagnetic flow meter (EMF, range: 0 to 250 cc/min) was used as a reference. Good linear relationships were found between the IVUS-derived flow and the calibrated EMF (all R 2> 0.96). The catheter position within the flow phantom and the size of the ring-down were theoretically analyzed. These elements, and noise in the RF signals, have an important influence on the IVUS blood flow measurements reflected by the offset and the slope of the linear relationships. By placing the IVUS catheter outside the flow phantom, parabolic blood flow profiles were also measured. Second, IVUS blood flow measurements were performed in the carotid artery of two Yorkshire pigs, which showed linear relationships (all R 2> 0.85) between the IVUS-derived flow and the calibrated EMF. Experimentally, the offset was lower than 3 mL/min and the slope was close to 1. Third, IVUS blood flow measurements were performed in coronary arteries in patients. Preliminary results for the coronary flow reserve (CFR = high flow/baseline flow) in patients using the decorrelation method of RF signals of an array IVUS scanner were comparable with CFR based on Doppler measurements. (E-mail: a.vandersteen@erasmusmc.nl)

Dynamic noise correction for IVUS quantitative volume blood flow: methods and numerical validation

In recent years, a new method to measure transverse blood flow based on the decorrelation of the radio-frequency (RF) signals, has been developed. Transverse blood flow estimation may be influenced by noise. In this paper, we investigated a new correlation-based method for noise correction. The decorrelation characteristics of transverse blood flow using an intravascular ultrasound (US) or IVUS array catheter were studied by means of computer modeling. Blood was simulated as a collection of randomly located point scatterers; moving this scattering medium transversely across the acoustical beam represented flow. Parabolic blood flow was simulated. Additive noise was added to the RF signals at a given signal-to-noise ratio (SNR). Next, a new method to dynamically estimate and suppress the decorrelation due to noise is presented. The decorrelation due to noise was estimated from the correlation coefficients from flowing blood obtained at increasing time lags. The correlation graphs are corrected for the decorrelation due to noise, leaving the decorrelation due to blood flow. The method shows promise to estimate and correct the correlation coefficients for noise. (E-mail:lupotti@tch.fgg.eur.n)

Decorrelation-based blood flow velocity estimation: effect of spread of flow velocity, linear flow velocity gradients, and parabolic flow.

In recent years, a new method to measure transverse blood flow, based on the decorrelation of the radio frequency (RF) signals has been developed. In this paper, we investigated the influence of nonuniform flow on the velocity estimation. The decorrelation characteristics of transverse blood flow using an intravascular ultrasound (IVUS) array catheter are studied by means of computer modeling. Blood was simulated as a collection of randomly located point scatterers; moving this scattering medium transversally across the acoustical beam represented flow. First-order statistics were evaluated, and the signal-to-noise ratio from the signals were measured. The correlation coefficient method was used to present the results. Three velocity profiles were simulated: random spread of blood-flow velocity, linear blood-flow velocity gradient, and parabolic blood-flow. Radio frequency and envelope signals were used to calculate the decorrelation pattern. The results were compared to the mean decorrelation pattern for plug blood-flow. The RF signals decorrelation patterns were in good agreement with those obtained for plug blood flow. Envelope decorrelation patterns show a close agreement with the one for plug blood flow. For axial blood flow, there is a discrepancy between decorrelation patterns. The results presented here suggest that the decorrelation properties of an IVUS array catheter for measuring quantitative transverse blood flow probably will not be affected by different transverse blood-flow conditions.

IVUS flow measurements: line spread function and decorrelation pattern

A method to measure transverse blood flow, based on correlation between consecutive radio frequency (RF) signals, has been developed. Currently, we are implementing the method for an intravascular (IVUS) array catheter. In this paper, the acoustical beam (line-spread function, LSF) was experimentally measured and compared with the simulated one. Next, the experimental LSF E was convolved with a matrix of white noise to produce RF E signals. Decorrelation pattern from the RF E signals was compared with the correspondent autoconvolution of the LSF E and a good agreement was found. We conclude that the transverse decorrelation pattern of the IVUS array catheter can be assessed from the properties of the acoustical beam.

Decorrelation characteristics of transverse blood flow along an intravascular array catheter: Effects of aggregation of red blood cells

A method to measure transverse blood flow, based on the correlation between consecutive radiofrequency (RF) signals, has been introduced. This method was validated for an intravascular (IVUS) rotating single element catheter. Currently, we are implementing the method for an IVUS array transducer catheter. The decorrelation characteristics during transverse blood flow using the IVUS array catheter were investigated using computer modeling. Before this, blood was simulated as a collection of randomly located point scatterers and, by moving this scattering medium transversely across the acoustical beam, blood flow was simulated. This paper presents a more realistic scattering media by simulating aggregates of red blood cells (RBCs) as strings of point scatterers. Three configurations of aggregates of RBCs were simulated. First, aggregates of RBCs were strings with different lengths and parallel to the catheter axis. Second, the strings were with a fixed length and angles of plus or minus 45° with respect to the catheter axis. Third, the strings were with different lengths and random angles ranging from −45° to + 45°. The decorrelation characteristics for these configurations of aggregates of RBCs were investigated and compared with point scatterers. For the aggregates of RBCs parallel to the catheter axis, the decorrelation rate became slower when the aggregate length was increased. RBC aggregations with fixed and random lengths and angles resulted in a decorrelation rate that approaches the decorrelation pattern from point scatterers. Results suggests that the presence of aggregates of RBCs will probably not affect the measurements of transverse blood flow using a decorrelation-based method and an IVUS array catheter. (E-mail: lupotti@tch.fgg.eur.nl)

Decorrelation characteristics of transverse blood flow along an intravascular array catheter

In recent years, a new method to measure transverse blood flow based on the decorrelation of radio frequency (RF) signals has been introduced. In this paper, we investigated the decorrelation characteristics of transverse blood flow measurement using an intravascular ultrasound (IVUS) array catheter by means of computer modeling. Blood was simulated as a collection of randomly located point scatterers. Moving this scattering medium transversally across the acoustical beam represented flow. First-order statistics were evaluated, and the signal-to-noise ratio from the signals was measured. The correlation coefficient method was used to present the results. The decorrelation patterns for RF and for RF-envelope signals were studied. The decorrelation patterns from the RF signals were in good agreement with those obtained from theoretical beam profiles. This agreement suggests that the decorrelation properties of an IVUS array catheter for measuring quantitative transverse blood flow can be assessed by measuring the ultrasound beam. A line of point scatterers, moved transversally across the acoustical beam (line spread function), can determine this decorrelation behaviour.