Multiple shoots were produced from nodal explants, of 30-day-old in vitro grown seedlings and from pretreated 3- and 9-month-old greenhouse grown Acacia mearnsii plants, respectively. Explants were sterilized using 0.1% and 0.2% HgCl2 for 15 min for 3- and 9-month-old explants, respectively. Nodal explants were induced to form multiple shoots when placed on MS medium supplemented with 2.0 mg l −1 benzyladenine. Rooting of these shoots was achieved on MS medium supplemented with 1.0 mg l −1 indole-3-butyric acid. Plantlets were acclimatized in transparent plastic containers under greenhouse conditions with a 90% success rate.