Translational Components Research Articles

Interferometric ISAR imaging under squint model using phase cancellation compensation method of combined processing data

This paper proposes a new interferometric inverse synthetic aperture radar (InISAR) image registration method under squint model based on phase cancellation compensation of combined processing data. Firstly, the residue translational motion component (RTMC) in range profile and the mismatching in the ISAR images induced by joint motion compensation with the same parameters to each antenna are analysed. Then, the RTMC compensation is applied to re-align the range profile sequences of the received antenna. The phase cancellation compensation is carried out to eliminate the mismatching in the range and azimuth direction, respectively. Simulation results demonstrate that the mismatching between the ISAR images can be compensated effectively, achieving high quality InISAR 3D images consequently.

Rapamycin, Acarbose and 17\u03b1-estradiol share common mechanisms regulating the MAPK pathways involved in intracellular signaling and inflammation

BackgroundRapamycin (Rapa), acarbose (ACA), and 17α-estradiol (17aE2, males only) have health benefits that increase lifespan of mice. Little is known about how these three agents alter the network of pathways downstream of insulin/IGF1 signals as well as inflammatory/stress responses.ResultsACA, Rapa, and 17aE2 (in males, but not in females) oppose age-related increases in the MEK1- ERK1/2-MNK1/2 cascade, and thus reduce phosphorylation of eIF4E, a key component of cap-dependent translation. In parallel, these treatments (in both sexes) reduce age-related increases in the MEK3-p38MAPK-MK2 pathway, to decrease levels of the acute phase response proteins involved in inflammation.ConclusionEach of three drugs converges on the regulation of both the ERK1/2 signaling pathway and the p38-MAPK pathway. The changes induced by treatments in ERK1/2 signaling are seen in both sexes, but the 17aE2 effects are male-specific, consistent with the effects on lifespan. However, the inhibition of age-dependent p38MAPK pathways and acute phase responses is triggered in both sexes by all three drugs, suggesting new approaches to prevention or reversal of age-related inflammatory changes in a clinical setting independent of lifespan effects.

Rayleigh-Wave Dispersion Analysis and Inversion Based on the Rotation.

Rotational observation is essential for a comprehensive description of the ground motion, and can provide additional wave-field information. With respect to the three typical layered models in shallow engineering geology, under the assumption of linear small deformation, we simulate the 2-dimensional radial, vertical, and rotational components of the wave fields and analyze the different characteristics of Rayleigh wave dispersion recorded for the rotational and translational components. Then, we compare the results of single-component inversion with the results of multi-component joint inversion. It is found that the rotational component has wider spectral bands and more higher modes than the translational components, especially at high frequencies; the rotational component has better anti-interference performance in the noisy data test, and it can improve the inversion accuracy of the shallow shear-wave velocity. The field examples also show the significant advantages of the joint utility of the translational and rotational components, especially when a low-velocity layer exists. Rotational observation shall be beneficial for shallow surface-wave exploration.

TDRD3 is an antiviral restriction factor that promotes IFN signaling with G3BP1.

Stress granules (SGs) are highly dynamic cytoplasmic foci that form in response to activation of the integrated stress response (ISR) that results in eIF2α phosphorylation and global translation shutdown. Stress granules, which are largely nucleated by G3BP1, serve as hubs for mRNA triage, but there is mounting evidence that they also perform cell signaling functions that are vital to cell survival, particularly during viral infection. We previously showed that SG formation leads to NFκB activation and JNK signaling and that this association may be due in part to G3BP1-dependent recruitment of PKR to SGs. Others have reported close associations between G3BP1 and various innate immune PRRs of the type 1 interferon signaling system, including RIG-I. We also reported SG assembly dynamics is dependent on the arginine-methylation status of G3BP1. Another protein that rapidly localizes to SGs, TDRD3, is a methyl reader protein that performs transcriptional activation and adaptor functions within the nucleus, but neither the mechanism nor its function in SGs is clear. Here, we present evidence that TDRD3 localizes to SGs partly based upon methylation potential of G3BP1. We also characterize granules that TDRD3 forms during overexpression and show that these granules can form in the absence of G3BP but also contain translation components found in canonical SGs. We also show for the first time that SGs recruit additional interferon effectors IRF3, IRF7, TBK1, and Sting, and provide evidence that TDRD3 may play a role in recruitment of these factors. We also present evidence that TDRD3 is a novel antiviral protein that is cleaved by enteroviral 2A proteinase. G3BP1 and TDRD3 knockdown in cells results in altered transcriptional regulation of numerous IFN effectors in complex modulatory patterns that are distinctive for G3BP1 and TDRD3. Overall, we describe a novel role of TDRD3 in innate immunity in which G3BP1 and TDRD3 may coordinate to play important roles in regulation of innate antiviral defenses.

Strain Response in the Facet Joint Capsule During Physiological Joint Rotation and Translation Following a Simulated Impact Exposure: An In Vitro Porcine Model.

Low back pain (LBP) is frequently reported following rear impact collisions. Knowledge of how the facet joint capsule (FJC) mechanically behaves before and after rear impact collisions may help explain LBP development despite negative radiographic evidence of gross tissue failure. This study quantified the Green strain tensor in the facet joint capsule during rotation and translation range-of-motion tests completed before and following an in vitro simulation of a rear impact collision. Eight FSUs (4 C3-C4, 4 C5-C6) were tested. Following a preload test, FSUs were flexed and extended at 0.5 deg/s until an ±8 N·m moment was achieved. Anterior and posterior joint translation was then applied at 0.2 mm/s until a target ±400 N shear load was imposed. Markers were drawn on the facet capsule surface and their coordinates were tracked during pre- and postimpact range-of-motion tests. Strain was defined as the change in point configuration relative to the determined neutral joint posture. There were no significant differences (p > 0.05) observed in all calculated FJC strain components in rotation and translation before and after the simulated impact. Our results suggest that LBP development resulting from the initiation of strain-induced mechanoreceptors and nociceptors with the facet joint capsule is unlikely following a severe rear impact collision within the boundaries of physiological joint motion.

Cascade or Direct Speech Translation? A Case Study

Speech translation has been traditionally tackled under a cascade approach, chaining speech recognition and machine translation components to translate from an audio source in a given language into text or speech in a target language. Leveraging on deep learning approaches to natural language processing, recent studies have explored the potential of direct end-to-end neural modelling to perform the speech translation task. Though several benefits may come from end-to-end modelling, such as a reduction in latency and error propagation, the comparative merits of each approach still deserve detailed evaluations and analyses. In this work, we compared state-of-the-art cascade and direct approaches on the under-resourced Basque–Spanish language pair, which features challenging phenomena such as marked differences in morphology and word order. This case study thus complements other studies in the field, which mostly revolve around the English language. We describe and analysed in detail the mintzai-ST corpus, prepared from the sessions of the Basque Parliament, and evaluated the strengths and limitations of cascade and direct speech translation models trained on this corpus, with variants exploiting additional data as well. Our results indicated that, despite significant progress with end-to-end models, which may outperform alternatives in some cases in terms of automated metrics, a cascade approach proved optimal overall in our experiments and manual evaluations.

Improving the Efficiency and Orthogonality of Genetic Code Expansion.

The site-specific incorporation of the noncanonical amino acid (ncAA) into proteins via genetic code expansion (GCE) has enabled the development of new and powerful ways to learn, regulate, and evolve biological functions in vivo. However, cellular biosynthesis of ncAA-containing proteins with high efficiency and fidelity is a formidable challenge. In this review, we summarize up-to-date progress towards improving the efficiency and orthogonality of GCE and enhancing intracellular compatibility of introduced translation machinery in the living cells by creation and optimization of orthogonal translation components, constructing genomically recoded organism (GRO), utilization of unnatural base pairs (UBP) and quadruplet codons (four-base codons), and spatial separation of orthogonal translation.

Analisis Algoritma Shi-Tomasi Dalam Pengujian Citra Senyum Pada Wajah Manusia

Problems in image processing to obtain the best smile are strongly influenced by the quality, background, position, and lighting, so it is very necessary to have an analysis by utilizing existing image processing algorithms to get a system that can make the best smile selection, then the Shi-Tomasi Algorithm is used. the algorithm that is commonly used to detect the corners of the smile region in facial images. The Shi-Tomasi angle calculation processes the image effectively from a target image in the edge detection ballistic test, then a corner point check is carried out on the estimation of translational parameters with a recreation test on the translational component to identify the cause of damage to the image, it is necessary to find the edge points to identify objects with remove noise in the image. The results of the test with the shi-Tomasi algorithm were used to detect a good smile from 20 samples of human facial images with each sample having 5 different smile images, with test data totaling 100 smile images, the success of the Shi-Tomasi Algorithm in detecting a good smile reached an accuracy value of 95% using the Confusion Matrix, Precision, Recall and Accuracy Methods.

Virtual Interdisciplinary Collaboration in Statewide Implementation of the MIND at Home Dementia Care Program

MIND at Home, a well-researched holistic, family-centered dementia care coordination program, provides collaborative support to community-dwelling persons living with dementia (PLWD) and their informal care partners (CP). Through comprehensive home-based assessment of 13 memory-care domains covering PLWD and CPs, individualized care plans are created, implemented, monitored, and revised over the course of the illness. Non-clinical Memory Care Coordinators (MCCs) working with an interdisciplinary team provide education and coaching to PLWD and their identified CP, and serve as a critical liaison and resource and between families, medical professional, and formal and informal community resources. This paper will describe a statewide pilot implementation of the program within a health plan across diverse sites in Texas and will present qualitative and quantitative descriptions of a key component of the program's effective translation to practice, the virtual collaborative case-based learning sessions. Health plan teams completed online interactive training modules and an intensive in-person case-based training with the Johns Hopkins team prior to program launch, and then engaged in weekly, hour-long virtual collaborative sessions that included health plan teams (site-based field teams, health plan clinical supervisory and specialty personnel [RNs, pharmacists, a geriatric psychiatrist, behavioral health specialists] and Johns Hopkins MIND program experts and geriatric psychiatrists. To date, the program has enrolled 350 health plan members, conducted 65 virtual collaborative sessions, and provided 423 CME/CEU units to team members. We will provide an overview of virtual collaborative session structure, participant contributions and discussion topics, case complexity, as well as didactic learning topics covered.

Gut microbiota and its influence on ovarian cancer carcinogenesis, anticancer therapy and surgical treatment: A literature review.

Ovarian cancer (OC) is the most lethal gynecological malignancy and very little is known about the underlying tumorigenesis mechanisms. For other tumors, like colorectal cancer, a relationship between several opportunistic pathogens and cancer development and progression has been proven. Recent researches also underline a possible correlation between gut microbiota dysbiosis and cancer treatment efficacy and adverse effects. Several studies have also demonstrated a link between abdominal surgery and gut microbiota modifications. In this paper, we aim to review the available evidences of this issue in OC to understand if there is a relationship between gut microbiota modifications and efficacy and adverse effects of cancer therapies, either surgical and medical treatments. Well-designed clinical studies, with a robust translational component, are required to better understand the modulation of gut microbiota during OC treatment. The microbiota/microbiome composition analysis, in the near future, could represent a novel instrument to personalize anticancer therapies.

Mitochondrial Plasticity Promotes Resistance to Sorafenib and Vulnerability to STAT3 Inhibition in Human Hepatocellular Carcinoma.

Simple SummaryEnhanced expression of mitochondrial ribosomal proteins and marked reprogramming of the mitochondrial network are associated with sorafenib resistance in human cell lines and hepatocarcinoma patients, providing novel actionable targets for increasing therapeutic efficacy.The multi-kinase inhibitor sorafenib is a primary treatment modality for advanced-stage hepatocellular carcinoma (HCC). However, the therapeutic benefits are short-lived due to innate and acquired resistance. Here, we examined how HCC cells respond to sorafenib and adapt to continuous and prolonged exposure to the drug. Sorafenib-adapted HCC cells show a profound reprogramming of mitochondria function and marked activation of genes required for mitochondrial protein translation and biogenesis. Mitochondrial ribosomal proteins and components of translation and import machinery are increased in sorafenib-resistant cells and sorafenib-refractory HCC patients show similar alterations. Sorafenib-adapted cells also exhibited increased serine 727 phosphorylated (pSer727) STAT3, the prevalent form in mitochondria, suggesting that STAT3 might be an actionable target to counteract resistance. Consistently, a small-molecule STAT3 inhibitor reduces pSer727, reverts mitochondrial alterations, and enhances the response to sorafenib in resistant cells. These results sustain the importance of mitochondria plasticity in response to sorafenib and identify a clinically actionable strategy for improving the treatment efficacy in HCC patients.

The Characteristics of Seismic Rotations in VTI Medium

Under the assumptions of linear elasticity and small deformation in traditional elastodynamics, the anisotropy of the medium has a significant effect on rotations observed during earthquakes. Based on the basic theory of the first-order velocity-stress elastic wave equation, this paper simulates the seismic wave propagation of the translational and rotational motions in two-dimensional isotropic and VTI (transverse isotropic media with a vertical axis of symmetry) media under different source mechanisms with the staggered-grid finite-difference method with respect to nine different seismological models. Through comparing the similarities and differences between the translational and rotational components of the wave fields, this paper focuses on the influence of anisotropic parameters on the amplitude and phase characteristics of the rotations. We verify that the energy of S waves in the rotational components is significantly stronger than that of P waves, and the response of rotations to the anisotropic parameters is more sensitive. There is more abundant information in the high-frequency band of the rotational components. With the increase of Thomsen anisotropic parameters ε and δ, the energy of the rotations increases gradually, which means that the rotational component observation may be helpful to the study of anisotropic parameters.

IDA-based seismic fragility of high-rise frame-core tube structure subjected to multi-dimensional long-period ground motions

Due to resonant effects, a lot of high-rise structures could be severely damaged under long-period seismic excitations. In this study, the seismic fragility method based on incremental dynamic analysis (IDA) is employed to quantitatively evaluate the damage levels of high-rise structures under long-period ground motions. Firstly, the frequency contents of special long-period ground motions are analyzed. Then, the finite element models of high-rise frame-core tube structures are established and verified. Lastly, the IDA curve clusters, quantile curves, probabilistic demand models, exceeding probability curve and seismic fragility matrix of the structure under multi-dimensional long-period ground motions are researched respectively. The analytical results show the amplitude contents of long-period ground motions are mainly distributed in the narrow low-frequencies band. Long-period earthquake is more likely to cause structural collapse than ordinary earthquake under large earthquake intensity, and the seismic fragility analysis based on a certain exceeding probability needs to consider the adverse effect of the increased horizontal translational components. The research conclusions will provide urgently needed theoretical basis for seismic design in areas with hidden long-period earthquake damage, and it also promote the development of seismic design methods and the improvement of seismic structural measures for high-rise structures.

On the Untranslatability of Cultural Aspects of Myth: An Investigation of Tolkien’s The Lord of the Rings

This study aims to explore the strategies which Persian translators of English myth works have adopted in dealing with cultural aspects. In addition, this study scrutinizes the culture-related problems which have led to untranslatability in myth translation. The analyzed parts were extracted from the first book of The Lord of the Rings (2012) by Tolkien and its two Persian translations by Alizadeh (2003) and Amini (2004). This comparative analysis was done based on the characteristics of adequate translation proposed by Newmark (1998) and Baker (1992), and also adequate discoursal translation components proposed by Lotfipour (2015). The most attention is on the translating of proper names, the genre of the work, and the author’s style about the cultural dimension of the work. The results revealed that the cultural knowledge of the translator about myth, origins, and features, affects the adequacy of translation. In addition, the main finding of this study proved that certain factors influence the translator’s performance to produce an adequate myth translation.

Representation of Body Orientation in Vestibular-Defective Patients Before and After Unilateral Vestibular Loss.

Introduction: The unilateral vestibular syndrome results in postural, oculomotor, perceptive, and cognitive symptoms. This study was designed to investigate the role of vestibular signals in body orientation representation, which remains poorly considered in vestibular patients.Methods: The subjective straight ahead (SSA) was investigated using a method disentangling translation and rotation components of error. Participants were required to align a rod with their body midline in the horizontal plane. Patients with right vestibular neurotomy (RVN; n =8) or left vestibular neurotomy (LVN; n = 13) or vestibular schwannoma resection were compared with 12 healthy controls. Patients were tested the day before surgery and during the recovery period, 7 days and 2 months after the surgery.Results: Before and after unilateral vestibular neurotomy, i.e., in the chronic phases, patients showed a rightward translation bias of their SSA, without rotation bias, whatever the side of the vestibular loss. However, the data show that the lower the translation error before neurotomy, the greater its increase 2 months after a total unilateral vestibular loss, therefore leading to a rightward translation of similar amplitude in the two groups of patients. In the early phase after surgery, SSA moved toward the operated side both in translation and in rotation, as typically found for biases occurring after unilateral vestibular loss, such as the subjective visual vertical (SVV) bias.Discussion and Conclusion: This study gives the first description of the immediate consequences and of the recovery time course of body orientation representation after a complete unilateral vestibular loss. The overall evolution differed according to the side of the lesion with more extensive changes over time before and after left vestibular loss. It is noteworthy that representational disturbances of self-orientation were highly unusual in the chronic stage after vestibular loss and similar to those reported after hemispheric lesions causing spatial neglect, while classical ipsilesional biases were reported in the acute stage. This study strongly supports the notion that the vestibular system plays a major role in body representation processes and more broadly in spatial cognition. From a clinical point of view, SSA appeared to be a reliable indicator for the presence of a vestibular disorder.

Multiplex suppression of four quadruplet codons via tRNA directed evolution

Genetic code expansion technologies supplement the natural codon repertoire with assignable variants in vivo, but are often limited by heterologous translational components and low suppression efficiencies. Here, we explore engineered Escherichia coli tRNAs supporting quadruplet codon translation by first developing a library-cross-library selection to nominate quadruplet codon–anticodon pairs. We extend our findings using a phage-assisted continuous evolution strategy for quadruplet-decoding tRNA evolution (qtRNA-PACE) that improved quadruplet codon translation efficiencies up to 80-fold. Evolved qtRNAs appear to maintain codon-anticodon base pairing, are typically aminoacylated by their cognate tRNA synthetases, and enable processive translation of adjacent quadruplet codons. Using these components, we showcase the multiplexed decoding of up to four unique quadruplet codons by their corresponding qtRNAs in a single reporter. Cumulatively, our findings highlight how E. coli tRNAs can be engineered, evolved, and combined to decode quadruplet codons, portending future developments towards an exclusively quadruplet codon translation system.

Fabricating a Low-Cost, Microscopy-Compatible Mechanical Testing Device.

Most commercially-available mechanical testing devices are bulky, expensive, and unable to evaluate changes in sample microstructure under load. This leaves a crucial gap in understanding between material structure and bulk mechanical properties. Our objective was to fabricate a mechanical testing device small enough to fit in most upright or inverted microscopy stages and able to position samples to allow for simultaneous mechanical and microstructural characterization. Parts were 3D printed using the hobbyist-friendly Fused Filament Fabrication technique, then assembled with commercial fasteners and translation components to create a mechanical testing device that utilized the deflection of plastic posts to determine sample reaction forces under applied strain. Video of sample deformation was analyzed using a custom processing script to calculate stress and strain behavior in an automated and high-throughput manner. This device was able to perform mechanical characterization with an accuracy comparable to commercial mechanical testing devices for a wide range of nonlinear and viscoelastic samples under dry and hydrated conditions. Additionally, the device showed compatibility with different upright and inverted microscopes and was able to demonstrate accurate mechanical testing results when used with these instruments. We successfully developed a device capable of accurately testing a majority of soft materials in the field of Biomedical Engineering with the ability to perform additional microstructural characterization using microscopy at a price point of $600.

ITIS and the Global Taxonomic Backbone

The Integrated Taxonomic Information System (ITIS) provides a regularly updated, global database that currently contains over 868,000 scientific names and their hierarchy. The program exists to communicate a comprehensive taxonomy of global species across 7 kingdoms that enables biodiversity information to be discovered, indexed, and connected across all human endeavors. ITIS partners with taxonomists and experts across the world to assemble scientific names and their taxonomic relationships, and then distributes that data through publicly available software. A single taxon may be represented by multiple scientific names, so ITIS makes it a priority to provide synonymy. Linking valid or accepted names with their subjective and objective synonyms is a key component of name translation and increases the precision of searches and organization of information. ITIS and its partner Species2000 create the Catalogue of Life (CoL) checklist that provides quality scientific name data for over 2.2M species. The CoL is the Global Biodiversity Information Facility (GBIF) taxonomic backbone. Providing automated open access to complete, current, literature-referenced, and expert-validated taxonomic information enables biological data management systems, and is elemental to enhancing the utility of the amassed scientific data across the world. Fully leveraging this information for the public good is crucial for empowering the global digital society to confront the most pressing social and environmental challenges.

Non-Canonical Translation Initiation Mechanisms Employed by Eukaryotic Viral mRNAs.

Viruses exploit the translation machinery of an infected cell to synthesize their proteins. Therefore, viral mRNAs have to compete for ribosomes and translation factors with cellular mRNAs. To succeed, eukaryotic viruses adopt multiple strategies. One is to circumvent the need for m7G-cap through alternative instruments for ribosome recruitment. These include internal ribosome entry sites (IRESs), which make translation independent of the free 5′ end, or cap-independent translational enhancers (CITEs), which promote initiation at the uncapped 5′ end, even if located in 3′ untranslated regions (3′ UTRs). Even if a virus uses the canonical cap-dependent ribosome recruitment, it can still perturb conventional ribosomal scanning and start codon selection. The pressure for genome compression often gives rise to internal and overlapping open reading frames. Their translation is initiated through specific mechanisms, such as leaky scanning, 43S sliding, shunting, or coupled termination-reinitiation. Deviations from the canonical initiation reduce the dependence of viral mRNAs on translation initiation factors, thereby providing resistance to antiviral mechanisms and cellular stress responses. Moreover, viruses can gain advantage in a competition for the translational machinery by inactivating individual translational factors and/or replacing them with viral counterparts. Certain viruses even create specialized intracellular “translation factories”, which spatially isolate the sites of their protein synthesis from cellular antiviral systems, and increase availability of translational components. However, these virus-specific mechanisms may become the Achilles’ heel of a viral life cycle. Thus, better understanding of the unconventional mechanisms of viral mRNA translation initiation provides valuable insight for developing new approaches to antiviral therapy.

The effect of rotational component of earthquake excitation on the response of steel structures

AbstractThis work is on the influence of the rotational component of earthquake excitations to the response of steel structures. In most studies, seismic input is being modeled only using the translational component of the ground acceleration, while the rotational one is ignored. This was due to the observation that the rotational component had minimal effect on low‐rise buildings. Hence, the accelerometers used would not measure it, leading to a lack of records. Nowadays, technology provides such instruments and relative records are made available. Indicative of that is that elastic design response spectra for rotational components are introduced to the design codes. In this paper, the results on structural response and internal forces due to the rotational component of a seismic excitation on the steel structures are examined. Dynamic time history analysis and response spectrum analysis of different steel structures are performed (a) considering the rotational component of the excitation and (b) without it. From the numerical results it is shown that the impact of rotational component in structural response and internal forces of the steel structures is significant and should not be ignored during structural design.